AT1G56450

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002350
Physical Location & Seq
Forward (+)
21141633 .. 21144458
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56450.1

Sequence Viewer

Length: 741 bp
ATGACGACTTTTTCTGTTCCGATTGATAACGGAGATTCTATGAAGATCGCAGAAGAAGAATCACAAAGAACACTGTACCCATATGTTACTGGAACTTCAATTGTTGCAATCAAGTACAAAGATGGGGTTTTGATGGCTTCTGATATGGGAGGTTCATATGGATCAACCTTAAGATACAAGAACATCGAGAGAGTGAAGGCTATTGGTAAGCATTCTCTTCTTGGTGCCAGCGGAGAAATCAGCGACTTTCAGGAGATTCTCCGTTATCTTGATGAGCTCACCCTGAATGATAACATGTGGGATGATGGTAACTCTTTGGGACCAAAAGAGATTCACAACTATCTGACCCGTGTGATGTACAACCGTCGCAACAAGTTTAACCCTCTGTGGAACACTCTCGTCCTTGGAGGCGTCAAAAATGGGAAAAGTTACCTTGGAATGGTGTCAATGATTGGTGTTAGTTTTGAGGACGATCATGTTGCCACTGGATTTGGAAACCACCTTGCTAGGCCAATTCTTCGTGATGAGTGGCATGCAGACCTGAGTTTTGAAGATGGTGTCAAGCTACTTGAGAAATGTATGCGTGTGCTTCTTTACCGTGACAGATCTGCTATTAACAAGCTTCAGATAGCGAAGATCACGGAAGAGGGCGTGACGGTCTCACAGCCATACTCATTGAAGACATACTGGGAATTCTCGTCATTCCACAACCCAACTGCTGGTGCTGCAGGCTCCTGGTAA

Protein Analysis

246

Amino Acids

27.65

Weight (kDa)

6.09

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 30 - 213 4.9e-35 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 719
AciI CCGC 1 cut(s) 231
AclWI GGATC 1 cut(s) 169
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 3 cut(s) 77, 116, 359
AfiI CCNNNNNNNGG 2 cut(s) 439, 719
AflII CTTAAG 1 cut(s) 169
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 3 cut(s) 99, 551, 679
AjnI CCWGG 1 cut(s) 734
AluBI AGCT 3 cut(s) 277, 565, 622
AluI AGCT 3 cut(s) 277, 565, 622
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 664
AlwI GGATC 1 cut(s) 169
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 725
ApoI RAATTY 1 cut(s) 692
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 271
AvaII GGWCC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 712
BccI CCATC 4 cut(s) 116, 127, 299, 548
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 736
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 726
BfrI CTTAAG 1 cut(s) 169
BglII AGATCT 1 cut(s) 605
BisI GCNGC 1 cut(s) 726
BlsI GCNGC 1 cut(s) 727
Bme1390I CCNGG 1 cut(s) 736
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 3 cut(s) 226, 321, 733
BmrFI CCNGG 1 cut(s) 736
BmrI ACTGGG 1 cut(s) 697
BmuI ACTGGG 1 cut(s) 697
BpiI GAAGAC 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 664
BsaJI CCNNGG 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 719
Bse1I ACTGG 3 cut(s) 94, 490, 692
BseBI CCWGG 1 cut(s) 736
BseDI CCNNGG 2 cut(s) 403, 433
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 439, 719
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 3 cut(s) 94, 490, 692
BseXI GCAGC 1 cut(s) 712
BshFI GGCC 1 cut(s) 511
BshNI GGYRCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 279
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 2 cut(s) 439, 719
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 333
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 1 cut(s) 511
Bso31I GGTCTC 1 cut(s) 664
Bsp1286I GDGCHC 1 cut(s) 279
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 5 cut(s) 45, 161, 472, 605, 636
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 1 cut(s) 534
BspLI GGNNCC 3 cut(s) 226, 321, 733
BspMAI CTGCAG 1 cut(s) 730
BspPI GGATC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 664
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 3 cut(s) 94, 490, 692
BssECI CCNNGG 2 cut(s) 403, 433
BssMI GATC 5 cut(s) 45, 161, 472, 605, 636
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 2 cut(s) 403, 433
Bst2UI CCWGG 1 cut(s) 736
Bst4CI ACNGT 4 cut(s) 75, 365, 599, 658
Bst6I CTCTTC 2 cut(s) 222, 639
BstACI GRCGYC 1 cut(s) 411
BstAFI CTTAAG 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 3 cut(s) 229, 534, 730
BstDEI CTNAG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 5 cut(s) 48, 164, 475, 608, 639
BstMAI GTCTC 1 cut(s) 664
BstMBI GATC 5 cut(s) 45, 161, 472, 605, 636
BstMWI GCNNNNNNNGC 1 cut(s) 725
BstNI CCWGG 1 cut(s) 736
BstNSI RCATGY 2 cut(s) 298, 536
BstSCI CCNGG 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 1 cut(s) 712
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 71, 483
Cac8I GCNNGC 3 cut(s) 229, 534, 730
Cfr13I GGNCC 1 cut(s) 320
CseI GACGC 1 cut(s) 400
Csp6I GTAC 3 cut(s) 76, 115, 358
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 3 cut(s) 295, 476, 533
CviJI RGCY 8 cut(s) 137, 200, 277, 511, 565, 622, 667, 732
CviKI_1 RGCY 8 cut(s) 137, 200, 277, 511, 565, 622, 667, 732
CviQI GTAC 3 cut(s) 76, 115, 358
DdeI CTNAG 1 cut(s) 542
DpnI GATC 5 cut(s) 47, 163, 474, 607, 638
DpnII GATC 5 cut(s) 45, 161, 472, 605, 636
Eam1104I CTCTTC 2 cut(s) 222, 639
EarI CTCTTC 2 cut(s) 222, 639
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 403, 433
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 734
EcoT14I CCWWGG 2 cut(s) 403, 433
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 2 cut(s) 403, 433
FaeI CATG 3 cut(s) 298, 479, 536
FaqI GGGAC 1 cut(s) 333
FatI CATG 3 cut(s) 294, 475, 532
FauNDI CATATG 2 cut(s) 82, 157
Fnu4HI GCNGC 1 cut(s) 726
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 726
FspBI CTAG 1 cut(s) 507
GluI GCNGC 1 cut(s) 726
HaeIII GGCC 1 cut(s) 511
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 3 cut(s) 298, 479, 536
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 4 cut(s) 35, 59, 256, 331
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 4 cut(s) 21, 142, 345, 627
Hpy188III TCNNGA 4 cut(s) 187, 251, 269, 521
Hpy99I CGWCG 1 cut(s) 369
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 4 cut(s) 75, 365, 599, 658
HpyCH4V TGCA 3 cut(s) 107, 536, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 725
HpyF3I CTNAG 1 cut(s) 542
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 3 cut(s) 298, 479, 536
Kzo9I GATC 5 cut(s) 45, 161, 472, 605, 636
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 1 cut(s) 712
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 5 cut(s) 85, 308, 428, 599, 652
MalI GATC 5 cut(s) 47, 163, 474, 607, 638
MboI GATC 5 cut(s) 45, 161, 472, 605, 636
MboII GAAGA 9 cut(s) 55, 65, 68, 209, 509, 563, 646, 656, 691
MfeI CAATTG 1 cut(s) 99
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 3 cut(s) 99, 513, 692
MnlI CCTC 5 cut(s) 143, 393, 401, 460, 640
MseI TTAA 3 cut(s) 170, 378, 615
MspA1I CMGCKG 1 cut(s) 231
MspCI CTTAAG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 736
MunI CAATTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 736
MwoI GCNNNNNNNGC 1 cut(s) 725
NdeI CATATG 2 cut(s) 82, 157
NdeII GATC 5 cut(s) 45, 161, 472, 605, 636
NlaIII CATG 3 cut(s) 298, 479, 536
NlaIV GGNNCC 3 cut(s) 226, 321, 733
NmuCI GTSAC 2 cut(s) 599, 652
NspI RCATGY 2 cut(s) 298, 536
PaeI GCATGC 1 cut(s) 536
PciI ACATGT 1 cut(s) 294
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 35, 59, 256, 331
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 1 cut(s) 727
PscI ACATGT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 734
PspGI CCWGG 1 cut(s) 734
PspN4I GGNNCC 3 cut(s) 226, 321, 733
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 1 cut(s) 605
RsaI GTAC 3 cut(s) 77, 116, 359
RsaNI GTAC 3 cut(s) 76, 115, 358
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 3 cut(s) 170, 378, 615
SatI GCNGC 1 cut(s) 726
Sau3AI GATC 5 cut(s) 45, 161, 472, 605, 636
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 1 cut(s) 736
SduI GDGCHC 1 cut(s) 279
SetI ASST 8 cut(s) 154, 170, 279, 435, 504, 543, 567, 624
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 169, 569
SmoI CTYRAG 2 cut(s) 169, 569
SphI GCATGC 1 cut(s) 536
Sse9I AATT 3 cut(s) 99, 513, 692
SsiI CCGC 1 cut(s) 231
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 734
StyI CCWWGG 2 cut(s) 403, 433
TaaI ACNGT 4 cut(s) 75, 365, 599, 658
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 99, 513, 692
TatI WGTACW 2 cut(s) 114, 357
TfiI GAWTC 4 cut(s) 35, 59, 256, 331
Tru1I TTAA 3 cut(s) 170, 378, 615
Tru9I TTAA 3 cut(s) 170, 378, 615
TscAI CASTG 2 cut(s) 78, 490
TseFI GTSAC 2 cut(s) 599, 652
TseI GCWGC 1 cut(s) 725
Tsp45I GTSAC 2 cut(s) 599, 652
TspDTI ATGAA 2 cut(s) 56, 144
TspGWI ACGGA 3 cut(s) 45, 251, 656
TspRI CASTG 2 cut(s) 78, 490
Van91I CCANNNNNTGG 1 cut(s) 719
Vha464I CTTAAG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 320
XapI RAATTY 1 cut(s) 692
XceI RCATGY 2 cut(s) 298, 536
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.