MD11G1131600.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002350
Physical Location & Seq
Reverse (-)
11904842 .. 11908799
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1131600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGTGGTATTTTGGGCTGTACCCATATGTGACTGGTACATCTGTTGTGGCTCTCAAGTACAAAGATGGAATCTTGATGGCTGCTGATATGGGAGGTTCTTATGGGTCTACCCTGCGATATAAGAGTGTGGAGCGAATGAAGCCCATTGGAAAGCATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGACTTTCAGGAGCTATTACGTTATCTTGATGAACTCATCCTATACGACAACATGTGGGATGATGGGAACTCTTTGGGACCGAAAGAGGTACACAACTATCTGACTCGTGTGATGTACAATAGGCGTAACAAGTTCAATCCACTGTGGAACTCTCTTGTCCTTGGTGGAGTAAAAAAGGGACAGAAGTATCTTGGGATGGTTAGCATGATAGGCGTGAATTTCGAGGACAATCATGTAGCAACTGGGTTTGGAAATCACCTTGCACGGCCTATTCTTCGTGATGAGTGGCATGAGAACTTGACTTTCGAAGAAGGTGTAAAGCTGCTGGAGAAATGCATGCGTGTCCTTCTCTATCGTGATCGGTCTGCTGTGAACAAGCTTCAGATATCTAAGATCACTGAAGAAGGCGTGACTATCTCGCAGCCCTACTCTTTGAAGACTTTCTGGGGTTTCTCCGCTTTCGAAAACCCAACATTGGGAGCTGAAGGATCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

228

Amino Acids

25.87

Weight (kDa)

7.78

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 11 - 192 1.1e-32 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 107
AciI CCGC 1 cut(s) 642
AclWI GGATC 1 cut(s) 682
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 2 cut(s) 551, 606
AfaI GTAC 5 cut(s) 20, 37, 59, 276, 302
AfiI CCNNNNNNNGG 2 cut(s) 661, 662
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 2 cut(s) 322, 622
AluBI AGCT 4 cut(s) 199, 508, 565, 668
AluI AGCT 4 cut(s) 199, 508, 565, 668
AlwI GGATC 1 cut(s) 682
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 3 cut(s) 80, 508, 607
ApoI RAATTY 1 cut(s) 403
Asp700I GAANNNNTTC 2 cut(s) 154, 626
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 434
AsuII TTCGAA 2 cut(s) 492, 648
AvaII GGWCC 1 cut(s) 263
BaeI ACNNNNGTAYC 4 cut(s) 27, 60, 356, 389
BauI CACGAG 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 629
BbvI GCAGC 3 cut(s) 67, 495, 619
BccI CCATC 4 cut(s) 59, 70, 242, 376
BceAI ACGGC 1 cut(s) 467
BisI GCNGC 3 cut(s) 81, 509, 608
BlsI GCNGC 3 cut(s) 82, 510, 609
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 264
BmrI ACTGGG 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 629
BpmI CTGGAG 1 cut(s) 533
Bpu14I TTCGAA 2 cut(s) 492, 648
BpuEI CTTGAG 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 346
Bsc4I CCNNNNNNNGG 2 cut(s) 661, 662
Bse1I ACTGG 2 cut(s) 37, 433
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 3 cut(s) 222, 250, 387
BseLI CCNNNNNNNGG 2 cut(s) 661, 662
BseNI ACTGG 2 cut(s) 37, 433
BseXI GCAGC 3 cut(s) 67, 495, 619
BshFI GGCC 1 cut(s) 454
BslFI GGGAC 2 cut(s) 276, 378
BslI CCNNNNNNNGG 2 cut(s) 661, 662
BsmFI GGGAC 2 cut(s) 276, 378
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 1 cut(s) 454
Bsp119I TTCGAA 2 cut(s) 492, 648
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 3 cut(s) 544, 579, 674
BspACI CCGC 1 cut(s) 642
BspANI GGCC 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 1 cut(s) 682
BspT104I TTCGAA 2 cut(s) 492, 648
BsrGI TGTACA 1 cut(s) 300
BsrI ACTGG 2 cut(s) 37, 433
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 3 cut(s) 544, 579, 674
BssSI CACGAG 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 330
Bst6I CTCTTC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 300
BstBI TTCGAA 2 cut(s) 492, 648
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 1 cut(s) 576
BstF5I GGATG 3 cut(s) 222, 250, 387
BstKTI GATC 3 cut(s) 547, 582, 677
BstMBI GATC 3 cut(s) 544, 579, 674
BstMWI GCNNNNNNNGC 2 cut(s) 139, 396
BstNSI RCATGY 2 cut(s) 241, 526
BstV1I GCAGC 3 cut(s) 67, 495, 619
BstV2I GAAGAC 1 cut(s) 629
BsuRI GGCC 1 cut(s) 454
BtsCI GGATG 3 cut(s) 222, 250, 387
BtsIMutI CAGTG 2 cut(s) 326, 582
Cac8I GCNNGC 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 5 cut(s) 19, 36, 58, 275, 301
CviAII CATG 6 cut(s) 238, 391, 419, 476, 523, 678
CviQI GTAC 5 cut(s) 19, 36, 58, 275, 301
DdeI CTNAG 1 cut(s) 576
DpnI GATC 3 cut(s) 546, 581, 676
DpnII GATC 3 cut(s) 544, 579, 674
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 346
Eco32I GATATC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 2 cut(s) 551, 606
EcoRV GATATC 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 6 cut(s) 241, 394, 422, 479, 526, 681
FaqI GGGAC 2 cut(s) 276, 378
FatI CATG 6 cut(s) 237, 390, 418, 475, 522, 677
FauNDI CATATG 1 cut(s) 25
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 3 cut(s) 81, 509, 608
FokI GGATG 3 cut(s) 209, 257, 394
Fsp4HI GCNGC 3 cut(s) 81, 509, 608
GluI GCNGC 3 cut(s) 81, 509, 608
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 1 cut(s) 454
Hin1II CATG 6 cut(s) 241, 394, 422, 479, 526, 681
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 2 cut(s) 69, 289
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 3 cut(s) 108, 277, 559
Hpy188I TCNGA 2 cut(s) 288, 570
Hpy188III TCNNGA 5 cut(s) 73, 194, 212, 464, 542
Hpy8I GTNNAC 3 cut(s) 108, 277, 559
HpyAV CCTTC 4 cut(s) 491, 542, 584, 665
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 170, 449, 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 396
HpyF3I CTNAG 1 cut(s) 576
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 6 cut(s) 241, 394, 422, 479, 526, 681
Kzo9I GATC 3 cut(s) 544, 579, 674
LmnI GCTCC 3 cut(s) 130, 196, 665
LpnPI CCDG 6 cut(s) 18, 125, 179, 414, 497, 616
Lsp1109I GCAGC 3 cut(s) 67, 495, 619
MaeII ACGT 1 cut(s) 205
MaeIII GTNAC 4 cut(s) 28, 185, 311, 595
MalI GATC 3 cut(s) 546, 581, 676
MboI GATC 3 cut(s) 544, 579, 674
MboII GAAGA 5 cut(s) 152, 452, 506, 599, 634
MluCI AATT 1 cut(s) 403
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 3 cut(s) 86, 265, 403
Mph1103I ATGCAT 1 cut(s) 524
MroXI GAANNNNTTC 2 cut(s) 154, 626
Mva1269I GAATGC 1 cut(s) 154
MwoI GCNNNNNNNGC 2 cut(s) 139, 396
NdeI CATATG 1 cut(s) 25
NdeII GATC 3 cut(s) 544, 579, 674
NlaIII CATG 6 cut(s) 241, 394, 422, 479, 526, 681
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 3 cut(s) 28, 185, 595
NsiI ATGCAT 1 cut(s) 524
NspI RCATGY 2 cut(s) 241, 526
NspV TTCGAA 2 cut(s) 492, 648
PaeI GCATGC 1 cut(s) 526
PciI ACATGT 1 cut(s) 237
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 154, 626
PfeI GAWTC 1 cut(s) 69
PkrI GCNGC 3 cut(s) 82, 510, 609
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PscI ACATGT 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 263
RsaI GTAC 5 cut(s) 20, 37, 59, 276, 302
RsaNI GTAC 5 cut(s) 19, 36, 58, 275, 301
SatI GCNGC 3 cut(s) 81, 509, 608
Sau3AI GATC 3 cut(s) 544, 579, 674
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 283
SetI ASST 9 cut(s) 97, 201, 208, 276, 447, 502, 510, 567, 670
SfuI TTCGAA 2 cut(s) 492, 648
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SphI GCATGC 1 cut(s) 526
Sse9I AATT 1 cut(s) 403
SsiI CCGC 1 cut(s) 642
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 330
TaiI ACGT 1 cut(s) 208
TaqI TCGA 3 cut(s) 408, 492, 648
TaqII GACCGA 2 cut(s) 280, 537
TasI AATT 1 cut(s) 403
TatI WGTACW 2 cut(s) 57, 300
TfiI GAWTC 1 cut(s) 69
TscAI CASTG 2 cut(s) 333, 589
TseFI GTSAC 3 cut(s) 28, 185, 595
TseI GCWGC 3 cut(s) 80, 508, 607
Tsp45I GTSAC 3 cut(s) 28, 185, 595
TspDTI ATGAA 2 cut(s) 152, 231
TspRI CASTG 2 cut(s) 333, 589
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 403
XceI RCATGY 2 cut(s) 241, 526
XmiI GTMKAC 1 cut(s) 107
XmnI GAANNNNTTC 2 cut(s) 154, 626
Zsp2I ATGCAT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.