Rorug05G0328900

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002350
Physical Location & Seq
Reverse (-)
39632982 .. 39637341
4360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0328900.1

Sequence Viewer

Length: 918 bp
ATGCAGCGAGTTACAAGGCCAAGAGGAGGCCTAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAGTTGGATTGCGTCGTAAGGAAGATGTGTATTTTCTTAATATATCGGGGGCAGAAAGTTGGGTCTCTACCAACAGAAAATGGTGGCAGTGCTGCTGGGTTGAAATGTGTCTTAGAAGCTGATGTCCAGGAGATTGTAACCATGGCTCGTCGGACTTATGAAAAGGATGGACCCATACTGGTGGTAGCTTGTGGCAGGGATACTATTTCCATTGTAAGTTCTATTAGACGACTAGCATCTGAAAACATCTTTGTTGTTCAGATACAACATCCCAGGTTGCATCTCAATAGATTTGACTTGGATTTTACCCCGAAACACGATTACTATCCTTTGACTCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCTTCGTGAACCTCCTGATAGGCATGTGGTCCTCACTATTGGAGCTTTGCATCAAATTGAGGCTACTGCCCTTCGTAATGCTGCCAGTGCATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTTGTGGTGAACATTGGTGGTCCTACAATCCACCCTTTTCATTTGACTGGCAATTGTCGTTATGGTGTGGACCTTGCGAAGCAATTGACAACCTATTTGCTTAGTGTTCTTGTAAGCTGTGGAAGCATCAGAATATGTTTCTCTAGTAGAACGCCTGAAAAGGTATCCAATTTTATAACAAAGGAGCTTGGTGGTAATCCAAAAGTTTACACTTGGGATGGTCAAGGGCTAGAGCCAAACCCGCACATGGGCCATCTAGCTTGGGTCGATGCTTTTGTTGTCACCGCAGATTCTGTTAGCATGATAAATGAGGTTTGCAGCACCGGGTATGGTTGA

Protein Analysis

305

Amino Acids

33.99

Weight (kDa)

8.89

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 37 - 303 1.1e-69 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
Acc36I ACCTGC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 546
AciI CCGC 2 cut(s) 824, 867
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 1 cut(s) 432
AfiI CCNNNNNNNGG 4 cut(s) 26, 546, 829, 830
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 198, 344
AluBI AGCT 7 cut(s) 191, 260, 500, 566, 699, 769, 842
AluI AGCT 7 cut(s) 191, 260, 500, 566, 699, 769, 842
Alw26I GTCTC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 875
AoxI GGCC 3 cut(s) 17, 28, 832
ApeKI GCWGC 5 cut(s) 4, 164, 536, 563, 900
ApoI RAATTY 1 cut(s) 557
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AspS9I GGNCC 5 cut(s) 242, 484, 602, 652, 832
AsuC2I CCSGG 2 cut(s) 56, 907
AsuHPI GGTGA 2 cut(s) 601, 856
AvaII GGWCC 4 cut(s) 242, 484, 602, 652
BbvI GCAGC 5 cut(s) 16, 151, 523, 575, 912
BccI CCATC 3 cut(s) 233, 794, 843
BciT130I CCWGG 2 cut(s) 200, 346
BciVI GTATCC 2 cut(s) 265, 757
BcnI CCSGG 2 cut(s) 56, 907
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 4 cut(s) 305, 726, 812, 839
BfuAI ACCTGC 1 cut(s) 415
BfuI GTATCC 2 cut(s) 265, 757
BisI GCNGC 5 cut(s) 5, 165, 537, 564, 901
BlsI GCNGC 5 cut(s) 6, 166, 538, 565, 902
Bme1390I CCNGG 4 cut(s) 56, 200, 346, 907
Bme18I GGWCC 4 cut(s) 242, 484, 602, 652
BmgT120I GGNCC 5 cut(s) 242, 484, 602, 652, 832
BmiI GGNNCC 3 cut(s) 53, 244, 429
BmrFI CCNGG 4 cut(s) 56, 200, 346, 907
BmsI GCATC 5 cut(s) 317, 361, 514, 717, 841
BpuMI CCSGG 2 cut(s) 56, 907
BsaAI YACGTR 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 213, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 546, 829, 830
Bse1I ACTGG 4 cut(s) 53, 255, 540, 634
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 200, 346
BseDI CCNNGG 2 cut(s) 213, 344
BseGI GGATG 3 cut(s) 244, 340, 805
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 26, 546, 829, 830
BseNI ACTGG 4 cut(s) 53, 255, 540, 634
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 5 cut(s) 16, 151, 523, 575, 912
BseYI CCCAGC 1 cut(s) 167
BshFI GGCC 3 cut(s) 19, 30, 834
BsiSI CCGG 2 cut(s) 56, 906
BslI CCNNNNNNNGG 4 cut(s) 26, 546, 829, 830
BsmAI GTCTC 1 cut(s) 141
BsnI GGCC 3 cut(s) 19, 30, 834
Bso31I GGTCTC 1 cut(s) 141
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 2 cut(s) 824, 867
BspANI GGCC 3 cut(s) 19, 30, 834
BspLI GGNNCC 3 cut(s) 53, 244, 429
BspMI ACCTGC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 141
BsrI ACTGG 4 cut(s) 53, 255, 540, 634
BssECI CCNNGG 2 cut(s) 213, 344
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 200, 346
Bst6I CTCTTC 1 cut(s) 462
BstBAI YACGTR 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 184, 683
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 3 cut(s) 244, 340, 805
BstMAI GTCTC 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 49, 542, 705, 823
BstNI CCWGG 2 cut(s) 200, 346
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 4 cut(s) 54, 198, 344, 905
BstV1I GCAGC 5 cut(s) 16, 151, 523, 575, 912
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuI GTATCC 2 cut(s) 265, 757
BsuRI GGCC 3 cut(s) 19, 30, 834
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 3 cut(s) 244, 340, 805
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 3 cut(s) 46, 166, 547
BveI ACCTGC 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 875
Cfr13I GGNCC 5 cut(s) 242, 484, 602, 652, 832
CseI GACGC 1 cut(s) 73
CviAII CATG 5 cut(s) 214, 479, 546, 829, 883
DdeI CTNAG 2 cut(s) 184, 683
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 4 cut(s) 242, 484, 602, 652
Eco57I CTGAAG 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 198, 344
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 5 cut(s) 217, 482, 549, 832, 886
FatI CATG 5 cut(s) 213, 478, 545, 828, 882
FauI CCCGC 1 cut(s) 831
Fnu4HI GCNGC 5 cut(s) 5, 165, 537, 564, 901
FokI GGATG 3 cut(s) 251, 327, 812
Fsp4HI GCNGC 5 cut(s) 5, 165, 537, 564, 901
FspBI CTAG 4 cut(s) 305, 726, 812, 839
GluI GCNGC 5 cut(s) 5, 165, 537, 564, 901
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 3 cut(s) 19, 30, 834
HapII CCGG 2 cut(s) 56, 906
HgaI GACGC 1 cut(s) 73
Hin1II CATG 5 cut(s) 217, 482, 549, 832, 886
HinfI GANTC 2 cut(s) 406, 872
HpaII CCGG 2 cut(s) 56, 906
HphI GGTGA 2 cut(s) 601, 856
Hpy166II GTNNAC 5 cut(s) 67, 464, 592, 652, 790
Hpy188I TCNGA 4 cut(s) 225, 313, 333, 713
Hpy188III TCNNGA 3 cut(s) 410, 461, 470
Hpy8I GTNNAC 5 cut(s) 67, 464, 592, 652, 790
Hpy99I CGWCG 2 cut(s) 89, 225
HpyAV CCTTC 1 cut(s) 536
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 6 cut(s) 4, 352, 505, 545, 563, 900
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 542, 705, 823
HpyF3I CTNAG 2 cut(s) 184, 683
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 5 cut(s) 217, 482, 549, 832, 886
LmnI GCTCC 3 cut(s) 57, 497, 766
Lsp1109I GCAGC 5 cut(s) 16, 151, 523, 575, 912
LweI GCATC 5 cut(s) 317, 361, 514, 717, 841
MaeI CTAG 4 cut(s) 305, 726, 812, 839
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 3 cut(s) 10, 208, 862
MboII GAAGA 2 cut(s) 106, 449
MfeI CAATTG 2 cut(s) 634, 665
MluCI AATT 6 cut(s) 434, 510, 557, 634, 665, 751
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 57, 203
MnlI CCTC 7 cut(s) 17, 20, 441, 477, 497, 508, 886
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 251
MspI CCGG 2 cut(s) 56, 906
MspR9I CCNGG 4 cut(s) 56, 200, 346, 907
MunI CAATTG 2 cut(s) 634, 665
MvaI CCWGG 2 cut(s) 200, 346
MwoI GCNNNNNNNGC 4 cut(s) 49, 542, 705, 823
NciI CCSGG 2 cut(s) 56, 907
NcoI CCATGG 1 cut(s) 213
NlaIII CATG 5 cut(s) 217, 482, 549, 832, 886
NlaIV GGNNCC 3 cut(s) 53, 244, 429
NmuCI GTSAC 1 cut(s) 862
NspI RCATGY 1 cut(s) 482
PceI AGGCCT 1 cut(s) 30
PfeI GAWTC 1 cut(s) 872
PflMI CCANNNNNTGG 1 cut(s) 546
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 5 cut(s) 6, 166, 538, 565, 902
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 443
PsiI TTATAA 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 198, 344
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 2 cut(s) 198, 344
PspN4I GGNNCC 3 cut(s) 53, 244, 429
PspPI GGNCC 5 cut(s) 242, 484, 602, 652, 832
PstNI CAGNNNCTG 1 cut(s) 875
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 5 cut(s) 5, 165, 537, 564, 901
Sau96I GGNCC 5 cut(s) 242, 484, 602, 652, 832
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 4 cut(s) 56, 200, 346, 907
SfaNI GCATC 5 cut(s) 317, 361, 514, 717, 841
SinI GGWCC 4 cut(s) 242, 484, 602, 652
SmiMI CAYNNNNRTG 1 cut(s) 251
Sse9I AATT 6 cut(s) 434, 510, 557, 634, 665, 751
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 2 cut(s) 824, 867
SspMI CTAG 4 cut(s) 305, 726, 812, 839
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 4 cut(s) 54, 198, 344, 905
StyI CCWWGG 1 cut(s) 213
TaiI ACGT 1 cut(s) 445
TaqI TCGA 1 cut(s) 849
TasI AATT 6 cut(s) 434, 510, 557, 634, 665, 751
TfiI GAWTC 1 cut(s) 872
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 3 cut(s) 53, 166, 547
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 5 cut(s) 4, 164, 536, 563, 900
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 3 cut(s) 246, 570, 611
TspRI CASTG 3 cut(s) 53, 166, 547
Van91I CCANNNNNTGG 1 cut(s) 546
VpaK11BI GGWCC 4 cut(s) 242, 484, 602, 652
XapI RAATTY 1 cut(s) 557
XceI RCATGY 1 cut(s) 482
XspI CTAG 4 cut(s) 305, 726, 812, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.