MD03G1112600.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002350
Physical Location & Seq
Reverse (-)
9894685 .. 9898486
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1112600.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGAGTCGAATCAATCCAAACCCGGCCTACTGTGCAATCCTGAAGTCTCTCAGAGAACCCTGTATCCATATGTGACTGGTACATCTGTTGTGGCTCTCAAGTACAAAGATGGAATTTTGATGGCTGCTGATATGGGAGGTTCTTATGGGTCTACCCTGCGGTACAAAAGTGTGGAGCGAATGAAGCCCGTTGGAAAGCATTCCCTTCTTGGCGCAAGTGGAGAAATAAGTGACTTTCAGGAGCTATTACGTTATCTTGACGAGCTCATCCTATACGACAACATGTGGGATGATGGGAACTCTTTGGGACCAAAAGAGGTGCACAACTATCTGACCCGTGTGATGTACAATAGGCGTAACAAGTTTGATCCACTGTGGAACTCTCTTGTCCTTGGTGGAGTAAAAAAGGGACAGAAGTACCTTGGCATGGTTAGCATGATAGGCGTGAATTTTGAGGATAATCATGTAGCTACAGGATTTGGAAATCACCTAGCACGGCCTATTCTTCGTGACGAGTGGCATGAGAACTTGACTTTTGAAGAAGGAGTAAAGTTGCTGGAGAAGTGCATGCGTGTCCTTCTCTATCGTGATCGATCTGCTGTCAACAAGCTTCAGATATCTAAAATCACTGAAGAAGGTGTGACTATCTCTCAGCCTTACTCTTTGAAGACTTTCTGGGGCTTCTCCGCTTTCGAAAATCCAACATTGGGCGCTGAAGGATCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

243

Amino Acids

27.37

Weight (kDa)

6.97

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 26 - 207 3.6e-32 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 152
AciI CCGC 2 cut(s) 160, 687
AclWI GGATC 2 cut(s) 362, 727
AcsI RAATTY 2 cut(s) 114, 448
AcuI CTGAAG 3 cut(s) 63, 596, 651
AfaI GTAC 5 cut(s) 82, 104, 164, 347, 419
AfiI CCNNNNNNNGG 2 cut(s) 427, 707
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 539, 667
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 4 cut(s) 244, 265, 470, 610
AluI AGCT 4 cut(s) 244, 265, 470, 610
Alw21I GWGCWC 2 cut(s) 267, 324
Alw26I GTCTC 1 cut(s) 52
Alw44I GTGCAC 1 cut(s) 320
AlwI GGATC 2 cut(s) 362, 727
AoxI GGCC 2 cut(s) 25, 497
ApaLI GTGCAC 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 2 cut(s) 114, 448
Asp700I GAANNNNTTC 2 cut(s) 199, 671
AspLEI GCGC 2 cut(s) 215, 713
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 693
AvaII GGWCC 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 324
BaeI ACNNNNGTAYC 6 cut(s) 72, 105, 154, 187, 401, 434
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 2 cut(s) 267, 324
BbvI GCAGC 1 cut(s) 112
BccI CCATC 3 cut(s) 104, 115, 287
BceAI ACGGC 1 cut(s) 512
BciVI GTATCC 1 cut(s) 75
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 714
BfuI GTATCC 1 cut(s) 75
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 707
Bse1I ACTGG 1 cut(s) 82
BseCI ATCGAT 1 cut(s) 592
BseDI CCNNGG 2 cut(s) 391, 421
BseGI GGATG 2 cut(s) 267, 295
BseLI CCNNNNNNNGG 2 cut(s) 427, 707
BseMII CTCAG 2 cut(s) 65, 665
BseNI ACTGG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 1 cut(s) 112
BshFI GGCC 2 cut(s) 27, 499
BshVI ATCGAT 1 cut(s) 592
BsiHKAI GWGCWC 2 cut(s) 267, 324
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 321, 423
BslI CCNNNNNNNGG 2 cut(s) 427, 707
BsmAI GTCTC 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 321, 423
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 2 cut(s) 27, 499
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 2 cut(s) 267, 324
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 4 cut(s) 367, 589, 593, 719
BspACI CCGC 2 cut(s) 160, 687
BspANI GGCC 2 cut(s) 27, 499
BspCNI CTCAG 2 cut(s) 64, 664
BspDI ATCGAT 1 cut(s) 592
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 2 cut(s) 362, 727
BspT104I TTCGAA 1 cut(s) 693
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 391, 421
BssMI GATC 4 cut(s) 367, 589, 593, 719
BssT1I CCWWGG 2 cut(s) 391, 421
Bst4CI ACNGT 2 cut(s) 33, 375
BstAUI TGTACA 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 569
BstDEI CTNAG 2 cut(s) 51, 651
BstF5I GGATG 2 cut(s) 267, 295
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 2 cut(s) 215, 713
BstKTI GATC 4 cut(s) 370, 592, 596, 722
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 4 cut(s) 367, 589, 593, 719
BstMWI GCNNNNNNNGC 4 cut(s) 33, 184, 432, 441
BstNSI RCATGY 2 cut(s) 286, 571
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 471
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 674
Bsu15I ATCGAT 1 cut(s) 592
BsuI GTATCC 1 cut(s) 75
BsuRI GGCC 2 cut(s) 27, 499
BsuTUI ATCGAT 1 cut(s) 592
BtsCI GGATG 2 cut(s) 267, 295
BtsIMutI CAGTG 2 cut(s) 371, 627
Cac8I GCNNGC 1 cut(s) 569
CfoI GCGC 2 cut(s) 215, 713
Cfr13I GGNCC 1 cut(s) 308
ClaI ATCGAT 1 cut(s) 592
Csp6I GTAC 5 cut(s) 81, 103, 163, 346, 418
CviAII CATG 7 cut(s) 283, 427, 436, 464, 521, 568, 723
CviQI GTAC 5 cut(s) 81, 103, 163, 346, 418
DdeI CTNAG 2 cut(s) 51, 651
DpnI GATC 4 cut(s) 369, 591, 595, 721
DpnII GATC 4 cut(s) 367, 589, 593, 719
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 391, 421
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 3 cut(s) 63, 596, 651
EcoICRI GAGCTC 1 cut(s) 265
EcoRV GATATC 1 cut(s) 618
EcoT14I CCWWGG 2 cut(s) 391, 421
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 391, 421
FaeI CATG 7 cut(s) 286, 430, 439, 467, 524, 571, 726
FaqI GGGAC 2 cut(s) 321, 423
FatI CATG 7 cut(s) 282, 426, 435, 463, 520, 567, 722
FauNDI CATATG 1 cut(s) 70
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 2 cut(s) 254, 302
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 491
GlaI GCGC 2 cut(s) 214, 712
GluI GCNGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 2 cut(s) 27, 499
HapII CCGG 1 cut(s) 24
HhaI GCGC 2 cut(s) 215, 713
Hin1II CATG 7 cut(s) 286, 430, 439, 467, 524, 571, 726
Hin6I GCGC 2 cut(s) 213, 711
HinP1I GCGC 2 cut(s) 213, 711
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 2 cut(s) 5, 10
HpaII CCGG 1 cut(s) 24
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 3 cut(s) 153, 322, 604
Hpy188I TCNGA 3 cut(s) 54, 333, 615
Hpy188III TCNNGA 5 cut(s) 41, 239, 257, 509, 587
Hpy8I GTNNAC 3 cut(s) 153, 322, 604
HpyAV CCTTC 5 cut(s) 215, 536, 587, 629, 710
HpyCH4III ACNGT 2 cut(s) 33, 375
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 36, 322, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 184, 432, 441
HpyF3I CTNAG 2 cut(s) 51, 651
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 7 cut(s) 286, 430, 439, 467, 524, 571, 726
HspAI GCGC 2 cut(s) 213, 711
Kzo9I GATC 4 cut(s) 367, 589, 593, 719
LmnI GCTCC 2 cut(s) 175, 241
LpnPI CCDG 9 cut(s) 37, 54, 63, 74, 170, 224, 459, 542, 661
Lsp1109I GCAGC 1 cut(s) 112
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 5 cut(s) 73, 230, 356, 509, 640
MalI GATC 4 cut(s) 369, 591, 595, 721
MboI GATC 4 cut(s) 367, 589, 593, 719
MboII GAAGA 4 cut(s) 497, 551, 644, 679
MhlI GDGCHC 2 cut(s) 267, 324
MluCI AATT 2 cut(s) 114, 448
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 172, 725
MnlI CCTC 3 cut(s) 131, 310, 448
MroXI GAANNNNTTC 2 cut(s) 199, 671
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 199
MwoI GCNNNNNNNGC 4 cut(s) 33, 184, 432, 441
NciI CCSGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 70
NdeII GATC 4 cut(s) 367, 589, 593, 719
NlaIII CATG 7 cut(s) 286, 430, 439, 467, 524, 571, 726
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 4 cut(s) 73, 230, 509, 640
NspI RCATGY 2 cut(s) 286, 571
NspV TTCGAA 1 cut(s) 693
PaeI GCATGC 1 cut(s) 571
PciI ACATGT 1 cut(s) 282
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 2 cut(s) 199, 671
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 1 cut(s) 308
RsaI GTAC 5 cut(s) 82, 104, 164, 347, 419
RsaNI GTAC 5 cut(s) 81, 103, 163, 346, 418
SacI GAGCTC 1 cut(s) 267
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 4 cut(s) 367, 589, 593, 719
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 2 cut(s) 267, 324
SfcI CTRYAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SphI GCATGC 1 cut(s) 571
Sse9I AATT 2 cut(s) 114, 448
SsiI CCGC 2 cut(s) 160, 687
SspMI CTAG 1 cut(s) 491
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 2 cut(s) 391, 421
TaaI ACNGT 2 cut(s) 33, 375
TaiI ACGT 1 cut(s) 253
TaqI TCGA 3 cut(s) 8, 592, 693
TasI AATT 2 cut(s) 114, 448
TatI WGTACW 2 cut(s) 102, 345
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 2 cut(s) 378, 634
TseFI GTSAC 4 cut(s) 73, 230, 509, 640
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 4 cut(s) 73, 230, 509, 640
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 2 cut(s) 378, 634
VneI GTGCAC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 2 cut(s) 114, 448
XceI RCATGY 2 cut(s) 286, 571
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 199, 671
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.