FvH4_7g09770

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002350
Physical Location & Seq
Forward (+)
9347673 .. 9348761
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09770.t1

Sequence Viewer

Length: 759 bp
ATGAACTCAATGGAACCGAACCAGGCGAAACTCAGCCTACTGTACTCCCCAGAAGCCTCTCAGTCTCAGAGAACACTGAATCCTAACGTGACTGGCACGTCTGTTGTGGCTCTCAAATACAAAGATGGAGTTTTGATGGCTGCAGATATGGGGGGTTCCTATGGATCCACCCTCCGATACAAGAGCATCGAACGAATGAAGCCTATCGGAAAGCATTCTCTCATCGGTGCAAGTGGAGAAATAAGTGATTTCCAGGAGGTGCTACGTTATCTTGATGAGCTCACCTTGCAGGACAGCATGTGTGATGATGGAAACTCTTTGGGACCTAAAGAGATCCACAACTACTTGAGCTGTGTGATGTACAACAGGCGTAACAAGTTTAATCCGCTGTGGAACTCGTTTGTCCTTGCTGGAGTGAAGAAGAAACCTGGAGTTGATAAGAGTGAGACATACCTTGGACTGGTGAATATGATAGGTGTGAACTTTGAGGATGATCATGTCGCTACTGGATTCGGAAACCACCTTGCACGGCCTATTCTTCGTGATGAATGGCGCGAGGACTTGAGTTTCGAAGAGGGTGTCAGGCTTCTGGAGAAATGTATGCGTGTGCTTTTGTATCGTGATAGGTCTGCTGTAAACAAGCTTCAGATAGCTAACATCACCGAAAAAGGTGTAACAATCTCTCAGCCTTACTCCTTGAAGACTTTCTGGGGGTTCTCTGCTTTCCAAAATCCAACAGCAGGTGCTCAAGGATCATGA

Protein Analysis

253

Amino Acids

28.12

Weight (kDa)

7.68

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 31 - 218 3.4e-29 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 731
AasI GACNNNNNNGTC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 731
AccII CGCG 1 cut(s) 555
AciI CCGC 1 cut(s) 386
AclWI GGATC 3 cut(s) 159, 172, 328
AcuI CTGAAG 1 cut(s) 629
AfaI GTAC 2 cut(s) 44, 362
AfiI CCNNNNNNNGG 2 cut(s) 460, 740
AgsI TTSAA 1 cut(s) 700
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 3 cut(s) 21, 252, 427
AloI GAACNNNNNNTCC 2 cut(s) 64, 96
AluBI AGCT 4 cut(s) 280, 351, 643, 653
AluI AGCT 4 cut(s) 280, 351, 643, 653
Alw21I GWGCWC 2 cut(s) 282, 748
Alw26I GTCTC 2 cut(s) 69, 440
AlwI GGATC 3 cut(s) 159, 172, 328
AoxI GGCC 1 cut(s) 530
ApeKI GCWGC 1 cut(s) 140
ArsI GACNNNNNNTTYG 4 cut(s) 551, 564, 583, 596
Asp700I GAANNNNTTC 2 cut(s) 214, 704
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 3 cut(s) 274, 475, 652
AsuII TTCGAA 1 cut(s) 570
AvaII GGWCC 1 cut(s) 323
BamHI GGATCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbsI GAAGAC 1 cut(s) 707
Bbv12I GWGCWC 2 cut(s) 282, 748
BbvI GCAGC 1 cut(s) 127
BccI CCATC 3 cut(s) 119, 130, 302
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 3 cut(s) 23, 254, 429
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 69, 440
BfmI CTRYAG 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 731
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 23, 254, 429
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 4 cut(s) 15, 157, 166, 324
BmrFI CCNGG 3 cut(s) 23, 254, 429
BmsI GCATC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 3 cut(s) 432, 450, 611
Bpu14I TTCGAA 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 367, 583, 732
BsaJI CCNNGG 1 cut(s) 454
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 740
Bse1I ACTGG 3 cut(s) 97, 465, 511
BseBI CCWGG 3 cut(s) 23, 254, 429
BseDI CCNNGG 1 cut(s) 454
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 2 cut(s) 460, 740
BseMII CTCAG 4 cut(s) 46, 74, 80, 698
BseNI ACTGG 3 cut(s) 97, 465, 511
BseXI GCAGC 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 555
BshFI GGCC 1 cut(s) 532
BsiHKAI GWGCWC 2 cut(s) 282, 748
BslFI GGGAC 1 cut(s) 336
BslI CCNNNNNNNGG 2 cut(s) 460, 740
BsmAI GTCTC 2 cut(s) 69, 440
BsmFI GGGAC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 532
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 2 cut(s) 282, 748
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 4 cut(s) 164, 333, 493, 752
BspACI CCGC 1 cut(s) 386
BspANI GGCC 1 cut(s) 532
BspCNI CTCAG 4 cut(s) 45, 73, 79, 697
BspFNI CGCG 1 cut(s) 555
BspHI TCATGA 1 cut(s) 755
BspLI GGNNCC 4 cut(s) 15, 157, 166, 324
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 731
BspPI GGATC 3 cut(s) 159, 172, 328
BspT104I TTCGAA 1 cut(s) 570
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 3 cut(s) 97, 465, 511
BssECI CCNNGG 1 cut(s) 454
BssMI GATC 4 cut(s) 164, 333, 493, 752
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 3 cut(s) 23, 254, 429
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 567
BstAUI TGTACA 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 570
BstDEI CTNAG 4 cut(s) 32, 60, 66, 684
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 1 cut(s) 555
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 4 cut(s) 167, 336, 496, 755
BstMAI GTCTC 2 cut(s) 69, 440
BstMBI GATC 4 cut(s) 164, 333, 493, 752
BstMWI GCNNNNNNNGC 1 cut(s) 286
BstNI CCWGG 3 cut(s) 23, 254, 429
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 3 cut(s) 21, 252, 427
BstSFI CTRYAG 1 cut(s) 141
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 2 cut(s) 164, 333
BstYI RGATCY 2 cut(s) 164, 333
BsuRI GGCC 1 cut(s) 532
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 74
BveI ACCTGC 1 cut(s) 731
CciI TCATGA 1 cut(s) 755
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 43, 361
CviAII CATG 3 cut(s) 298, 497, 756
CviQI GTAC 2 cut(s) 43, 361
DdeI CTNAG 4 cut(s) 32, 60, 66, 684
DpnI GATC 4 cut(s) 166, 335, 495, 754
DpnII GATC 4 cut(s) 164, 333, 493, 752
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 629
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 21, 252, 427
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 3 cut(s) 301, 500, 759
FaiI YATR 8 cut(s) 149, 162, 299, 451, 470, 498, 602, 757
FaqI GGGAC 1 cut(s) 336
FatI CATG 3 cut(s) 297, 496, 755
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 554
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 3 cut(s) 432, 450, 611
HaeIII GGCC 1 cut(s) 532
HhaI GCGC 1 cut(s) 555
Hin1II CATG 3 cut(s) 301, 500, 759
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 2 cut(s) 79, 510
HphI GGTGA 3 cut(s) 274, 475, 652
Hpy166II GTNNAC 2 cut(s) 481, 637
Hpy188I TCNGA 5 cut(s) 69, 176, 209, 515, 648
Hpy188III TCNNGA 5 cut(s) 272, 542, 590, 620, 756
Hpy8I GTNNAC 2 cut(s) 481, 637
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 3 cut(s) 87, 98, 265
HpyCH4V TGCA 4 cut(s) 143, 230, 289, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 286
HpyF3I CTNAG 4 cut(s) 32, 60, 66, 684
HpySE526I ACGT 3 cut(s) 87, 98, 265
Hsp92II CATG 3 cut(s) 301, 500, 759
HspAI GCGC 1 cut(s) 553
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 4 cut(s) 164, 333, 493, 752
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 195
MaeII ACGT 3 cut(s) 87, 98, 265
MaeIII GTNAC 3 cut(s) 88, 371, 673
MalI GATC 4 cut(s) 166, 335, 495, 754
MboI GATC 4 cut(s) 164, 333, 493, 752
MboII GAAGA 5 cut(s) 430, 433, 530, 584, 712
MflI RGATCY 2 cut(s) 164, 333
MhlI GDGCHC 2 cut(s) 282, 748
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 6 cut(s) 67, 182, 250, 481, 550, 568
MroXI GAANNNNTTC 2 cut(s) 214, 704
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 388
MspR9I CCNGG 3 cut(s) 23, 254, 429
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 3 cut(s) 23, 254, 429
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 1 cut(s) 286
NdeII GATC 4 cut(s) 164, 333, 493, 752
NlaIII CATG 3 cut(s) 301, 500, 759
NlaIV GGNNCC 4 cut(s) 15, 157, 166, 324
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 1 cut(s) 301
NspV TTCGAA 1 cut(s) 570
PagI TCATGA 1 cut(s) 755
PaqCI CACCTGC 1 cut(s) 731
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 214, 704
PfeI GAWTC 2 cut(s) 79, 510
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 21, 252, 427
PspGI CCWGG 3 cut(s) 21, 252, 427
PspN4I GGNNCC 4 cut(s) 15, 157, 166, 324
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 164, 333
RsaI GTAC 2 cut(s) 44, 362
RsaNI GTAC 2 cut(s) 43, 361
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 164, 333, 493, 752
Sau96I GGNCC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 23, 254, 429
SduI GDGCHC 2 cut(s) 282, 748
SfaNI GCATC 1 cut(s) 195
SfcI CTRYAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 570
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 346, 562, 747
SmoI CTYRAG 3 cut(s) 346, 562, 747
SsiI CCGC 1 cut(s) 386
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 21, 252, 427
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 3 cut(s) 90, 101, 268
TaqI TCGA 2 cut(s) 189, 570
TatI WGTACW 2 cut(s) 42, 360
TfiI GAWTC 2 cut(s) 79, 510
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 3 cut(s) 17, 212, 561
TspRI CASTG 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 323
XceI RCATGY 1 cut(s) 301
XmnI GAANNNNTTC 2 cut(s) 214, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.