pycom02g10950

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002350
Physical Location & Seq
Reverse (-)
7740685 .. 7742718
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10950.1

Sequence Viewer

Length: 609 bp
ATGGCTGCTGATATGGGAGGTTCTTATGGCTCTACCCTGCGATACAAGAGTGTGGAAAGAATGAAGCCAATTGGAAAGCATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGACTTTCAGGAGCTCTTACGTTATCTTGATGAACTTGTCCTGTATGACAACATGTGGGATGATGGGAACTCTTTGGGACCTAAAGAGGTGCACAACTATTTGACCCGTGTGATGTACAATAGGCGTAACAAGTTCAATCCATTGTGGAACTCTCTTGTCCTCGGTGGAGTGAAAAATGGACAGAAGTACCTTGGCATGGTTAGCATGATAGGTGTAAATTTTGAGGACAATCATGTGGCTACGGGATTTGGAAATCACCTTGCACGGCCTATTCTTCGTGATGAGTGGCATGAGAACTTGACTTTCGAAGAGGCTGTAAAGCTGCTGGAGAAGTGCATGCGTGTCCTTCTCTATCGAGATCGGTCTGCTGTGAACAAGCTTCAGATAGCTAAAATCACAGAAGAAGGCGTAACAATTTCACAGCCCTACTCTTTGAAGACATTCTGGGGTTTCTCTGCGTTCGAGAATCCAACATTGGGCGCTGAAGGATCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

203

Amino Acids

22.97

Weight (kDa)

6.53

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 1 - 169 1.5e-25 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 607
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 2 cut(s) 227, 299
AfiI CCNNNNNNNGG 2 cut(s) 307, 587
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 2 cut(s) 247, 547
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 124, 433, 490, 500
AluI AGCT 4 cut(s) 124, 433, 490, 500
Alw21I GWGCWC 2 cut(s) 126, 204
Alw44I GTGCAC 1 cut(s) 200
AlwI GGATC 1 cut(s) 607
AoxI GGCC 1 cut(s) 377
ApaLI GTGCAC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 5, 433
ApoI RAATTY 1 cut(s) 328
Asp700I GAANNNNTTC 2 cut(s) 79, 551
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 417
AvaII GGWCC 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 204
BaeI ACNNNNGTAYC 4 cut(s) 34, 67, 281, 314
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 554
Bbv12I GWGCWC 2 cut(s) 126, 204
BbvI GCAGC 1 cut(s) 420
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 392
BfoI RGCGCY 1 cut(s) 594
BisI GCNGC 2 cut(s) 6, 434
BlsI GCNGC 2 cut(s) 7, 435
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 1 cut(s) 458
Bpu14I TTCGAA 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 587
BseDI CCNNGG 2 cut(s) 271, 301
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 307, 587
BseSI GKGCMC 1 cut(s) 204
BseXI GCAGC 1 cut(s) 420
BshFI GGCC 1 cut(s) 379
BsiHKAI GWGCWC 2 cut(s) 126, 204
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 307, 587
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 126, 204
Bsp1407I TGTACA 1 cut(s) 225
Bsp143I GATC 2 cut(s) 469, 599
BspANI GGCC 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 1 cut(s) 607
BspT104I TTCGAA 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 225
BssECI CCNNGG 2 cut(s) 271, 301
BssMI GATC 2 cut(s) 469, 599
BssT1I CCWWGG 1 cut(s) 301
Bst6I CTCTTC 2 cut(s) 90, 414
BstAUI TGTACA 1 cut(s) 225
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 449
BstF5I GGATG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 2 cut(s) 472, 602
BstMBI GATC 2 cut(s) 469, 599
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNSI RCATGY 2 cut(s) 166, 451
BstSLI GKGCMC 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 420
BstV2I GAAGAC 1 cut(s) 554
BsuRI GGCC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 449
CfoI GCGC 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 226, 298
CviAII CATG 7 cut(s) 163, 307, 316, 344, 401, 448, 603
CviQI GTAC 2 cut(s) 226, 298
DpnI GATC 2 cut(s) 471, 601
DpnII GATC 2 cut(s) 469, 599
Eam1104I CTCTTC 2 cut(s) 90, 414
EarI CTCTTC 2 cut(s) 90, 414
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
FaqI GGGAC 1 cut(s) 201
FatI CATG 7 cut(s) 162, 306, 315, 343, 400, 447, 602
Fnu4HI GCNGC 2 cut(s) 6, 434
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 6, 434
GlaI GCGC 1 cut(s) 592
GluI GCNGC 2 cut(s) 6, 434
GsuI CTGGAG 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 1 cut(s) 379
HhaI GCGC 1 cut(s) 593
Hin1II CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 577
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 202, 484
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 5 cut(s) 119, 137, 389, 467, 574
Hpy8I GTNNAC 2 cut(s) 202, 484
HpyAV CCTTC 3 cut(s) 467, 509, 590
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 4 cut(s) 95, 202, 374, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
HspAI GCGC 1 cut(s) 591
Kzo9I GATC 2 cut(s) 469, 599
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 5 cut(s) 50, 104, 164, 422, 541
Lsp1109I GCAGC 1 cut(s) 420
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 110, 236, 520
MalI GATC 2 cut(s) 471, 601
MboI GATC 2 cut(s) 469, 599
MboII GAAGA 5 cut(s) 77, 377, 431, 524, 559
MfeI CAATTG 1 cut(s) 69
MhlI GDGCHC 2 cut(s) 126, 204
MluCI AATT 3 cut(s) 69, 328, 525
MmeI TCCRAC 1 cut(s) 605
MnlI CCTC 5 cut(s) 11, 190, 281, 328, 415
MroXI GAANNNNTTC 2 cut(s) 79, 551
MunI CAATTG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 312
NdeII GATC 2 cut(s) 469, 599
NlaIII CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 2 cut(s) 166, 451
NspV TTCGAA 1 cut(s) 417
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 1 cut(s) 162
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 79, 551
PfeI GAWTC 1 cut(s) 577
PkrI GCNGC 2 cut(s) 7, 435
PpuMI RGGWCCY 1 cut(s) 188
PscI ACATGT 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 188
PspPPI RGGWCCY 1 cut(s) 188
RsaI GTAC 2 cut(s) 227, 299
RsaNI GTAC 2 cut(s) 226, 298
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 2 cut(s) 6, 434
Sau3AI GATC 2 cut(s) 469, 599
Sau96I GGNCC 1 cut(s) 188
SduI GDGCHC 2 cut(s) 126, 204
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 1 cut(s) 188
SphI GCATGC 1 cut(s) 451
Sse9I AATT 3 cut(s) 69, 328, 525
SstI GAGCTC 1 cut(s) 126
StyI CCWWGG 1 cut(s) 301
TaiI ACGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 417, 466, 573
TaqII GACCGA 1 cut(s) 462
TasI AATT 3 cut(s) 69, 328, 525
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 1 cut(s) 577
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 5, 433
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 77, 156
VneI GTGCAC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 1 cut(s) 328
XceI RCATGY 2 cut(s) 166, 451
XmnI GAANNNNTTC 2 cut(s) 79, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.