Rw5G037060

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002350
Physical Location & Seq
Forward (+)
65288854 .. 65291945
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037060.1

Sequence Viewer

Length: 648 bp
ATGGCTAAACTAAGCATTGATGTTAATAATGTGCATGATTATTTTTCGAACCAAGCCAATCCCAGCATACTGTGCCGCCCAGAAGGCTCTCAGAGAACCCTGTACCCATATGTCACTGGTACTTCTGTAGTTGCTCTCAAGTACAAAGATGGGATTTTGATGGCTTGGAAAAACGGAAAACATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGATTTCCAGGAGAGAAATACCTTACCTTTATGGTATGCTTCTCTTTTAGGATTTTTAACAGCCTATATGACAACATTTGAGCTGGGACTTTTGACCCATGATCATGTTTGCTTGTGGAGTATCGTCCTTGGAATGGTTAGTATGATAGGTGTAAATTTTGAGGACAACCATGTTGCTACCGGATTTGGGAATCACCTTGCAAGGCCTATTCTTCGTGATGAGTGGCGTGAGGACTTGAGTTTCGAAGAAGGCGTGAAGCTGCTGGAGAAGTGCATGCGTGTCCTTCTGTACCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAAGATCACTGGAGAAGGTGTAACAATCTCTCAACCCTACTCTTTGAAGACTTTCTGGGGATTCTCTGCTTTCCAAAATCCAACTGCCGGTGCTGAAGGATCATGGTAA

Protein Analysis

215

Amino Acids

24.09

Weight (kDa)

6.3

Isoelectric Point (pI)

28.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 114 - 182 3.4e-10 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 76
AclWI GGATC 1 cut(s) 646
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 4 cut(s) 104, 121, 143, 503
AfiI CCNNNNNNNGG 3 cut(s) 346, 399, 626
AgsI TTSAA 1 cut(s) 586
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 4 cut(s) 295, 472, 529, 539
AluI AGCT 4 cut(s) 295, 472, 529, 539
AlwI GGATC 1 cut(s) 646
AoxI GGCC 1 cut(s) 416
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 1 cut(s) 367
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
Asp700I GAANNNNTTC 2 cut(s) 181, 590
AsuHPI GGTGA 1 cut(s) 398
AsuII TTCGAA 2 cut(s) 47, 456
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 1 cut(s) 459
BccI CCATC 2 cut(s) 143, 154
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 313
BfmI CTRYAG 1 cut(s) 126
BglI GCCNNNNNGGC 1 cut(s) 84
BisI GCNGC 2 cut(s) 76, 473
BlsI GCNGC 2 cut(s) 77, 474
Bme1390I CCNGG 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 593
BpmI CTGGAG 2 cut(s) 497, 570
Bpu14I TTCGAA 2 cut(s) 47, 456
BpuEI CTTGAG 2 cut(s) 122, 469
BsaJI CCNNGG 1 cut(s) 340
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 399, 626
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 121, 553
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 346, 399, 626
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 121, 553
BseXI GCAGC 1 cut(s) 459
BseYI CCCAGC 2 cut(s) 62, 295
BshFI GGCC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 393, 627
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 346, 399, 626
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 1 cut(s) 418
Bsp119I TTCGAA 2 cut(s) 47, 456
Bsp143I GATC 3 cut(s) 313, 543, 638
BspACI CCGC 1 cut(s) 76
BspANI GGCC 1 cut(s) 418
BspCNI CTCAG 1 cut(s) 103
BspPI GGATC 1 cut(s) 646
BspT104I TTCGAA 2 cut(s) 47, 456
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 2 cut(s) 121, 553
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 3 cut(s) 313, 543, 638
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 72, 506
Bst6I CTCTTC 1 cut(s) 192
BstAPI GCANNNNNTGC 1 cut(s) 72
BstBI TTCGAA 2 cut(s) 47, 456
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 3 cut(s) 11, 90, 540
BstKTI GATC 3 cut(s) 316, 546, 641
BstMBI GATC 3 cut(s) 313, 543, 638
BstMWI GCNNNNNNNGC 2 cut(s) 72, 84
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 219
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 459
BstV2I GAAGAC 1 cut(s) 593
BsuRI GGCC 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 114, 546
Cac8I GCNNGC 1 cut(s) 488
Cfr10I RCCGGY 1 cut(s) 626
Csp6I GTAC 4 cut(s) 103, 120, 142, 502
CviAII CATG 6 cut(s) 35, 311, 317, 383, 487, 642
CviQI GTAC 4 cut(s) 103, 120, 142, 502
DdeI CTNAG 3 cut(s) 11, 90, 540
DpnI GATC 3 cut(s) 315, 545, 640
DpnII GATC 3 cut(s) 313, 543, 638
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 6 cut(s) 38, 314, 320, 386, 490, 645
FaqI GGGAC 1 cut(s) 312
FatI CATG 6 cut(s) 34, 310, 316, 382, 486, 641
FauNDI CATATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 76, 473
Fsp4HI GCNGC 2 cut(s) 76, 473
GluI GCNGC 2 cut(s) 76, 473
GsaI CCCAGC 2 cut(s) 66, 299
GsuI CTGGAG 2 cut(s) 497, 570
HaeIII GGCC 1 cut(s) 418
HapII CCGG 2 cut(s) 393, 627
Hin1II CATG 6 cut(s) 38, 314, 320, 386, 490, 645
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 2 cut(s) 403, 600
HpaII CCGG 2 cut(s) 393, 627
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 2 cut(s) 93, 534
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 1 cut(s) 523
HpyAV CCTTC 5 cut(s) 77, 455, 506, 548, 629
HpyCH4III ACNGT 2 cut(s) 72, 506
HpyCH4V TGCA 4 cut(s) 34, 197, 413, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 84
HpyF3I CTNAG 3 cut(s) 11, 90, 540
Hsp92II CATG 6 cut(s) 38, 314, 320, 386, 490, 645
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 3 cut(s) 313, 543, 638
Lsp1109I GCAGC 1 cut(s) 459
MaeIII GTNAC 2 cut(s) 112, 559
MalI GATC 3 cut(s) 315, 545, 640
MboI GATC 3 cut(s) 313, 543, 638
MboII GAAGA 4 cut(s) 179, 416, 470, 598
MluCI AATT 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 2 cut(s) 367, 436
MroXI GAANNNNTTC 2 cut(s) 181, 590
MseI TTAA 2 cut(s) 24, 269
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 2 cut(s) 393, 627
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 72, 84
NdeI CATATG 1 cut(s) 109
NdeII GATC 3 cut(s) 313, 543, 638
NlaIII CATG 6 cut(s) 38, 314, 320, 386, 490, 645
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 490
NspV TTCGAA 2 cut(s) 47, 456
PaeI GCATGC 1 cut(s) 490
PceI AGGCCT 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PdmI GAANNNNTTC 2 cut(s) 181, 590
PfeI GAWTC 2 cut(s) 403, 600
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 2 cut(s) 77, 474
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 2 cut(s) 62, 295
PspGI CCWGG 1 cut(s) 219
RsaI GTAC 4 cut(s) 104, 121, 143, 503
RsaNI GTAC 4 cut(s) 103, 120, 142, 502
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 2 cut(s) 24, 269
SatI GCNGC 2 cut(s) 76, 473
Sau3AI GATC 3 cut(s) 313, 543, 638
ScrFI CCNGG 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 126
SfuI TTCGAA 2 cut(s) 47, 456
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 137, 448
SmoI CTYRAG 2 cut(s) 137, 448
SphI GCATGC 1 cut(s) 490
Sse9I AATT 1 cut(s) 367
SseBI AGGCCT 1 cut(s) 418
SsiI CCGC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 418
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 72, 506
TaqI TCGA 2 cut(s) 47, 456
TasI AATT 1 cut(s) 367
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 2 cut(s) 403, 600
Tru1I TTAA 2 cut(s) 24, 269
Tru9I TTAA 2 cut(s) 24, 269
TscAI CASTG 2 cut(s) 121, 553
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 112
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 2 cut(s) 121, 553
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 490
XmnI GAANNNNTTC 2 cut(s) 181, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.