RLG00000035357

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002350
Physical Location & Seq
Forward (+)
65780281 .. 65785100
4820 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035357

Sequence Viewer

Length: 858 bp
ATGAAGTCAAAACCCACCGTCGTCCCCGGAAGAGCGATTAAAGAGAAGAGAACATCTTACAGTTCCGATCATCGTGAACTATTTTCTGTCCCTCGGCTGCAAATCTCGTATCACACAAAAATTAACTTAATGGAGTTGAACCAAGCCAATCCCAGCATACTGTGCAGCCCAGAAGGCTCTCAGAGAACCCTGTACCCATATGTCACTGGTACTTCTGTAGTGGCTCTCAAGTACAAAGATGGGATTTTGATGGCTGCTGATATGGGAGGTTCATATGGATCTACCCTGCGGTACAAGAGTGTGGAACGAATGAAGCCTATTGGAAAGCATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGATTTCCAGGAGTTGTTAAAATATCTTGATGAGCTTGTCCTATATGACAACATGTGGGATGATGGGAACTCTTTGGGACCTAAAGAGATCCACAACTATTTGACCCGTGTGATGTACAATCGCCGTAACAAGTTCAACCCACTGTGGAATTCACTTGTCCTTGGTGGAGTGAAAAACGGAGAGAAATACCTTGGAATGGTTAGTATGATAGGTGTAAATTTCGAGGACAACCATGTTGCTACCGGATTTGGGAATCACCTTGCAAGGCCTATTCTTCGTGATGAGTGGCGTGAGGACTTGAGTTTCGAAGAAGGCGTGAAGCTGCTGGAGAAGTGCATGCGTGTTCTTCTGTACCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAAGATCACTGGAGAAGGTGTAACAATCTCTCAACCCTACTCTTTGAAGACTTTCTGGGGATTCTCTGCTTTCCAAAATCCAACTGCCGGTGCTGAAGGATCATGGTAA

Protein Analysis

286

Amino Acids

32.16

Weight (kDa)

9.11

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 69 - 252 1.1e-32 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 289
AclWI GGATC 3 cut(s) 286, 442, 856
AcsI RAATTY 2 cut(s) 508, 577
AcuI CTGAAG 1 cut(s) 725
AfaI GTAC 6 cut(s) 194, 211, 233, 293, 476, 713
AfiI CCNNNNNNNGG 3 cut(s) 556, 609, 836
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 3 cut(s) 139, 496, 796
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 4 cut(s) 394, 682, 739, 749
AluI AGCT 4 cut(s) 394, 682, 739, 749
AlwI GGATC 3 cut(s) 286, 442, 856
AoxI GGCC 1 cut(s) 626
ApeKI GCWGC 4 cut(s) 97, 165, 254, 682
ApoI RAATTY 2 cut(s) 508, 577
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
Asp700I GAANNNNTTC 2 cut(s) 328, 800
AspS9I GGNCC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 608
AsuII TTCGAA 1 cut(s) 666
AvaII GGWCC 1 cut(s) 437
BaeI ACNNNNGTAYC 4 cut(s) 201, 234, 283, 316
BbsI GAAGAC 1 cut(s) 803
BbvI GCAGC 4 cut(s) 84, 177, 241, 669
BccI CCATC 3 cut(s) 233, 244, 416
BceAI ACGGC 1 cut(s) 468
BciT130I CCWGG 1 cut(s) 368
BcnI CCSGG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 216
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 4 cut(s) 98, 166, 255, 683
BlsI GCNGC 4 cut(s) 99, 167, 256, 684
Bme1390I CCNGG 2 cut(s) 27, 368
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 27, 368
BpiI GAAGAC 1 cut(s) 803
BpmI CTGGAG 2 cut(s) 707, 780
Bpu14I TTCGAA 1 cut(s) 666
BpuEI CTTGAG 2 cut(s) 212, 679
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 25, 92, 520, 550
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 3 cut(s) 556, 609, 836
Bse118I RCCGGY 1 cut(s) 836
Bse1I ACTGG 2 cut(s) 211, 763
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 4 cut(s) 25, 92, 520, 550
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 3 cut(s) 556, 609, 836
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 211, 763
BseXI GCAGC 4 cut(s) 84, 177, 241, 669
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 184
BshFI GGCC 1 cut(s) 628
BsiSI CCGG 3 cut(s) 27, 603, 837
BslFI GGGAC 3 cut(s) 8, 74, 450
BslI CCNNNNNNNGG 3 cut(s) 556, 609, 836
BsmFI GGGAC 3 cut(s) 8, 74, 450
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 628
Bsp119I TTCGAA 1 cut(s) 666
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 5 cut(s) 67, 278, 447, 753, 848
BspACI CCGC 1 cut(s) 289
BspANI GGCC 1 cut(s) 628
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 438
BspPI GGATC 3 cut(s) 286, 442, 856
BspQI GCTCTTC 1 cut(s) 25
BspT104I TTCGAA 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 836
BsrGI TGTACA 1 cut(s) 474
BsrI ACTGG 2 cut(s) 211, 763
BssAI RCCGGY 1 cut(s) 836
BssECI CCNNGG 4 cut(s) 25, 92, 520, 550
BssMI GATC 5 cut(s) 67, 278, 447, 753, 848
BssT1I CCWWGG 2 cut(s) 520, 550
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 5 cut(s) 19, 62, 162, 504, 716
Bst6I CTCTTC 3 cut(s) 25, 41, 339
BstAPI GCANNNNNTGC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 666
BstC8I GCNNGC 1 cut(s) 698
BstDEI CTNAG 2 cut(s) 180, 750
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 5 cut(s) 70, 281, 450, 756, 851
BstMBI GATC 5 cut(s) 67, 278, 447, 753, 848
BstMWI GCNNNNNNNGC 2 cut(s) 162, 174
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 2 cut(s) 415, 700
BstSCI CCNGG 2 cut(s) 25, 366
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 4 cut(s) 84, 177, 241, 669
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 2 cut(s) 278, 447
BstYI RGATCY 2 cut(s) 278, 447
BsuRI GGCC 1 cut(s) 628
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 3 cut(s) 204, 500, 756
Cac8I GCNNGC 1 cut(s) 698
Cfr10I RCCGGY 1 cut(s) 836
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 6 cut(s) 193, 210, 232, 292, 475, 712
CviAII CATG 4 cut(s) 412, 593, 697, 852
CviQI GTAC 6 cut(s) 193, 210, 232, 292, 475, 712
DdeI CTNAG 2 cut(s) 180, 750
DpnI GATC 5 cut(s) 69, 280, 449, 755, 850
DpnII GATC 5 cut(s) 67, 278, 447, 753, 848
Eam1104I CTCTTC 3 cut(s) 25, 41, 339
EarI CTCTTC 3 cut(s) 25, 41, 339
Eco130I CCWWGG 2 cut(s) 520, 550
Eco147I AGGCCT 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 2 cut(s) 520, 550
ErhI CCWWGG 2 cut(s) 520, 550
FaeI CATG 4 cut(s) 415, 596, 700, 855
FaqI GGGAC 3 cut(s) 8, 74, 450
FatI CATG 4 cut(s) 411, 592, 696, 851
FauNDI CATATG 2 cut(s) 199, 274
Fnu4HI GCNGC 4 cut(s) 98, 166, 255, 683
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 4 cut(s) 98, 166, 255, 683
GluI GCNGC 4 cut(s) 98, 166, 255, 683
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 707, 780
HaeIII GGCC 1 cut(s) 628
HapII CCGG 3 cut(s) 27, 603, 837
Hin1II CATG 4 cut(s) 415, 596, 700, 855
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 2 cut(s) 613, 810
HpaII CCGG 3 cut(s) 27, 603, 837
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 2 cut(s) 77, 733
Hpy188I TCNGA 3 cut(s) 67, 183, 744
Hpy188III TCNNGA 3 cut(s) 74, 386, 638
Hpy8I GTNNAC 2 cut(s) 77, 733
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 167, 665, 758, 839
HpyCH4III ACNGT 5 cut(s) 19, 62, 162, 504, 716
HpyCH4V TGCA 5 cut(s) 100, 165, 344, 623, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 174
HpyF3I CTNAG 2 cut(s) 180, 750
Hsp92II CATG 4 cut(s) 415, 596, 700, 855
Kzo9I GATC 5 cut(s) 67, 278, 447, 753, 848
LguI GCTCTTC 1 cut(s) 25
Lsp1109I GCAGC 4 cut(s) 84, 177, 241, 669
MaeIII GTNAC 3 cut(s) 202, 485, 769
MalI GATC 5 cut(s) 69, 280, 449, 755, 850
MboI GATC 5 cut(s) 67, 278, 447, 753, 848
MboII GAAGA 7 cut(s) 42, 58, 326, 626, 680, 698, 808
MflI RGATCY 2 cut(s) 278, 447
MluCI AATT 3 cut(s) 120, 508, 577
MmeI TCCRAC 1 cut(s) 854
MnlI CCTC 4 cut(s) 102, 260, 577, 646
MroXI GAANNNNTTC 2 cut(s) 328, 800
MseI TTAA 4 cut(s) 39, 123, 128, 377
MspI CCGG 3 cut(s) 27, 603, 837
MspR9I CCNGG 2 cut(s) 27, 368
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 162, 174
NciI CCSGG 1 cut(s) 27
NdeI CATATG 2 cut(s) 199, 274
NdeII GATC 5 cut(s) 67, 278, 447, 753, 848
NlaIII CATG 4 cut(s) 415, 596, 700, 855
NlaIV GGNNCC 1 cut(s) 438
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 2 cut(s) 415, 700
NspV TTCGAA 1 cut(s) 666
PaeI GCATGC 1 cut(s) 700
PceI AGGCCT 1 cut(s) 628
PciI ACATGT 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 1 cut(s) 328
PdmI GAANNNNTTC 2 cut(s) 328, 800
PfeI GAWTC 2 cut(s) 613, 810
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 4 cut(s) 99, 167, 256, 684
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 1 cut(s) 437
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 2 cut(s) 278, 447
RsaI GTAC 6 cut(s) 194, 211, 233, 293, 476, 713
RsaNI GTAC 6 cut(s) 193, 210, 232, 292, 475, 712
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 4 cut(s) 39, 123, 128, 377
SatI GCNGC 4 cut(s) 98, 166, 255, 683
Sau3AI GATC 5 cut(s) 67, 278, 447, 753, 848
Sau96I GGNCC 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 27, 368
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 666
SinI GGWCC 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 227, 658
SmoI CTYRAG 2 cut(s) 227, 658
SphI GCATGC 1 cut(s) 700
Sse9I AATT 3 cut(s) 120, 508, 577
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 1 cut(s) 289
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 25, 366
StyI CCWWGG 2 cut(s) 520, 550
TaaI ACNGT 5 cut(s) 19, 62, 162, 504, 716
TaqI TCGA 2 cut(s) 582, 666
TasI AATT 3 cut(s) 120, 508, 577
TatI WGTACW 2 cut(s) 231, 474
TfiI GAWTC 2 cut(s) 613, 810
Tru1I TTAA 4 cut(s) 39, 123, 128, 377
Tru9I TTAA 4 cut(s) 39, 123, 128, 377
TscAI CASTG 3 cut(s) 211, 507, 763
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 4 cut(s) 97, 165, 254, 682
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 3 cut(s) 17, 261, 326
TspGWI ACGGA 1 cut(s) 552
TspRI CASTG 3 cut(s) 211, 507, 763
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 2 cut(s) 508, 577
XceI RCATGY 2 cut(s) 415, 700
XmnI GAANNNNTTC 2 cut(s) 328, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.