MD15G1251900.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002350
Physical Location & Seq
Reverse (-)
21235546 .. 21238374
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1251900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGAGTTTGGTGGAGTCGAACCAATCCAAACCCGGCCTACTGTGCAATCCTGAAGGCTCGCAGAGAACCCTGTACCCATATGTAACAGGTACATCTGTTGTGGCTCTCAAGTATAAAGATGGAATTTTGATGGCTGCTGATATGGGAGGTTCATATGGGTCTACCCTGCGATACAAGAGTGTGGAGCGAATGAAGCCAATTGGAAAGCATTCTGTTCTTGGTGCAAGTGGAGAAATAAGTGACTTTCAGGAGTTATTACATTATCTGGATGAGCTCGTCCTGTACGACAACATGTGGAATGATGGGAACTCTTTGGGACCTAAAGAGGTGCACAACTATTTGACCCGTGTGATGTACAATAGGCGTAACAAGTTCAATCCGTTGTGGAACTCTCTTGTCCTTGGTGGAGTGAAAAATGGACAGAAGTATCTTGGCATGGTTAGCATGATAGGTGTCAATTTTGAGGATGATCATGTAGCTACGGGATTTGGAAATCACCTTGCACGGCCTATTCTTCGCGATGAGTGGCATGAGAACTTGACTTTCGAAGAGGGTGTAAAGCTGCTGGAGAAATGCATGCGTGTCCTTCTCTATCGAGATCGGTCTGCTGTGAACAAGCTTCAGATAGCTAAAATCACTGAAGAAGGTGCAACGGTCTCTCAGCCCTACTCTTTGAAGACGTTCTGGGGTTTCTCTGCTTTCGAGAATCCAACATTGGGTGCTGAGGGGTCATGGTAG

Protein Analysis

246

Amino Acids

27.53

Weight (kDa)

6.6

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 29 - 212 1.4e-32 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AccII CGCG 1 cut(s) 519
AcsI RAATTY 1 cut(s) 123
AcuI CTGAAG 3 cut(s) 72, 605, 660
AfaI GTAC 4 cut(s) 74, 91, 284, 356
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 2 cut(s) 376, 676
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 5 cut(s) 274, 479, 562, 619, 629
AluI AGCT 5 cut(s) 274, 479, 562, 619, 629
Alw21I GWGCWC 2 cut(s) 276, 333
Alw26I GTCTC 1 cut(s) 661
Alw44I GTGCAC 1 cut(s) 329
AoxI GGCC 2 cut(s) 34, 506
ApaLI GTGCAC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 134, 562
ApoI RAATTY 1 cut(s) 123
Asp700I GAANNNNTTC 2 cut(s) 208, 680
AspS9I GGNCC 1 cut(s) 317
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 488
AsuII TTCGAA 1 cut(s) 546
AvaII GGWCC 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 333
BaeI ACNNNNGTAYC 6 cut(s) 81, 114, 163, 196, 410, 443
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 276, 333
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 121, 549
BccI CCATC 3 cut(s) 113, 124, 296
BceAI ACGGC 1 cut(s) 521
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 661
BisI GCNGC 2 cut(s) 135, 563
BlsI GCNGC 2 cut(s) 136, 564
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 683
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 723
Bpu14I TTCGAA 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 1 cut(s) 400
Bsc4I CCNNNNNNNGG 1 cut(s) 716
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 2 cut(s) 274, 472
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 2 cut(s) 674, 714
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 2 cut(s) 121, 549
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 2 cut(s) 36, 508
BsiHKAI GWGCWC 2 cut(s) 276, 333
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 2 cut(s) 36, 508
Bso31I GGTCTC 1 cut(s) 661
Bsp119I TTCGAA 1 cut(s) 546
Bsp1286I GDGCHC 2 cut(s) 276, 333
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 2 cut(s) 469, 598
Bsp68I TCGCGA 1 cut(s) 519
BspANI GGCC 2 cut(s) 36, 508
BspCNI CTCAG 2 cut(s) 673, 715
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 661
BsrGI TGTACA 1 cut(s) 354
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 2 cut(s) 469, 598
BssT1I CCWWGG 1 cut(s) 400
Bst4CI ACNGT 2 cut(s) 42, 655
Bst6I CTCTTC 1 cut(s) 543
BstAUI TGTACA 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 546
BstC8I GCNNGC 2 cut(s) 59, 578
BstDEI CTNAG 2 cut(s) 660, 723
BstF5I GGATG 2 cut(s) 274, 472
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 2 cut(s) 472, 601
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 2 cut(s) 469, 598
BstMWI GCNNNNNNNGC 3 cut(s) 42, 193, 441
BstNSI RCATGY 2 cut(s) 295, 580
BstSCI CCNGG 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 121, 549
BstV2I GAAGAC 1 cut(s) 683
BsuRI GGCC 2 cut(s) 36, 508
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 274, 472
BtsIMutI CAGTG 1 cut(s) 636
BtuMI TCGCGA 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 59, 578
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 4 cut(s) 73, 90, 283, 355
CviAII CATG 7 cut(s) 292, 436, 445, 473, 530, 577, 732
CviQI GTAC 4 cut(s) 73, 90, 283, 355
DdeI CTNAG 2 cut(s) 660, 723
DpnI GATC 2 cut(s) 471, 600
DpnII GATC 2 cut(s) 469, 598
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 3 cut(s) 72, 605, 660
EcoICRI GAGCTC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 7 cut(s) 295, 439, 448, 476, 533, 580, 735
FaqI GGGAC 1 cut(s) 330
FatI CATG 7 cut(s) 291, 435, 444, 472, 529, 576, 731
FauNDI CATATG 2 cut(s) 79, 154
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 135, 563
FokI GGATG 2 cut(s) 281, 479
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 135, 563
GluI GCNGC 2 cut(s) 135, 563
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 2 cut(s) 36, 508
HapII CCGG 1 cut(s) 33
Hin1II CATG 7 cut(s) 295, 439, 448, 476, 533, 580, 735
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 2 cut(s) 14, 706
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 3 cut(s) 162, 331, 613
Hpy188I TCNGA 1 cut(s) 624
Hpy188III TCNNGA 6 cut(s) 50, 248, 266, 518, 596, 703
Hpy8I GTNNAC 3 cut(s) 162, 331, 613
HpyAV CCTTC 3 cut(s) 47, 596, 638
HpyCH4III ACNGT 2 cut(s) 42, 655
HpyCH4IV ACGT 1 cut(s) 680
HpyCH4V TGCA 6 cut(s) 45, 224, 331, 503, 576, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 193, 441
HpyF3I CTNAG 2 cut(s) 660, 723
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 7 cut(s) 295, 439, 448, 476, 533, 580, 735
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 2 cut(s) 469, 598
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 121, 549
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 3 cut(s) 82, 239, 365
MalI GATC 2 cut(s) 471, 600
MboI GATC 2 cut(s) 469, 598
MboII GAAGA 4 cut(s) 506, 560, 653, 688
MfeI CAATTG 1 cut(s) 198
MhlI GDGCHC 2 cut(s) 276, 333
MluCI AATT 3 cut(s) 123, 198, 457
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 5 cut(s) 140, 319, 457, 544, 718
Mph1103I ATGCAT 1 cut(s) 578
MroXI GAANNNNTTC 2 cut(s) 208, 680
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 33
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 208
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 3 cut(s) 42, 193, 441
NciI CCSGG 1 cut(s) 33
NdeI CATATG 2 cut(s) 79, 154
NdeII GATC 2 cut(s) 469, 598
NlaIII CATG 7 cut(s) 295, 439, 448, 476, 533, 580, 735
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 239
NruI TCGCGA 1 cut(s) 519
NsiI ATGCAT 1 cut(s) 578
NspI RCATGY 2 cut(s) 295, 580
NspV TTCGAA 1 cut(s) 546
PaeI GCATGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 208
PdmI GAANNNNTTC 2 cut(s) 208, 680
PfeI GAWTC 1 cut(s) 706
PkrI GCNGC 2 cut(s) 136, 564
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PpuMI RGGWCCY 1 cut(s) 317
PscI ACATGT 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 317
PspPPI RGGWCCY 1 cut(s) 317
RruI TCGCGA 1 cut(s) 519
RsaI GTAC 4 cut(s) 74, 91, 284, 356
RsaNI GTAC 4 cut(s) 73, 90, 283, 355
SacI GAGCTC 1 cut(s) 276
SatI GCNGC 2 cut(s) 135, 563
Sau3AI GATC 2 cut(s) 469, 598
Sau96I GGNCC 1 cut(s) 317
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 2 cut(s) 276, 333
SfuI TTCGAA 1 cut(s) 546
SinI GGWCC 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SphI GCATGC 1 cut(s) 580
Sse9I AATT 3 cut(s) 123, 198, 457
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 2 cut(s) 42, 655
TaiI ACGT 1 cut(s) 683
TaqI TCGA 4 cut(s) 17, 546, 595, 702
TaqII GACCGA 1 cut(s) 591
TasI AATT 3 cut(s) 123, 198, 457
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 1 cut(s) 706
TscAI CASTG 1 cut(s) 643
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 2 cut(s) 134, 562
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 141, 206
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 643
VneI GTGCAC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 1 cut(s) 123
XceI RCATGY 2 cut(s) 295, 580
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 2 cut(s) 208, 680
Zsp2I ATGCAT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.