FvH4_3g33020

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002350
Physical Location & Seq
Forward (+)
28368651 .. 28372650
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33020.t2

Sequence Viewer

Length: 729 bp
ATGGAGTCGAACCAAGCCAATCCGAGCATACTGTGCAACCCAGAAGGCTCCCAGAGAACCCTGTACCCATATGTCACTGGTACATCTGTAGTGGCTCTCAAGTACAGAGATGGGATTTTGATGGCTGCTGATATGGGAGGCTCATATGGGTCTACCCTGCGGTACAAGAGTGTGGAACGCATGAAGCCTATTGGAAAGCATTCTCTTCTTGGCGCAAGTGGAGAAATAAGTGATTTCCAGGAGTTGTTAAAATATCTTGATGAGCTCGTCCTATATGACAACATGTGGGATGATGGGAACTCTTTGGGACCTAAAGAGATCCACAACTATTTGACTCGTGTGATGTACAATCGGCGTAACAAGTTCAATCCACTGTGGAATTCACTTGTCCTTGGTGGAGTGAAGAACGGAAAGCAATACCTTGGAATGGTTAGTATGATTGGTGTAAATTTTGAGGACGACCATGTTGCTACTGGATTTGGGAATCACCTTGCAAGGCCTATTCTTCGTGATGAGTGGCGTGCGGACTTGAGTTTCGAAGAAGGCGTGAAGCTGCTGGAGAAGTGCATGCGTGTCCTTCTGTACCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAAGATCACTGAAGAGGGTGTAACAATCTCTAAACCCTACGCTTTGAAGACTTTCTGGGGATACTCTGCTTTCCAAAATCCAACTGCTGGTGCTGAAGGATCATGGTAA

Protein Analysis

243

Amino Acids

27.22

Weight (kDa)

7.72

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 26 - 209 3.4e-32 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 152
AciI CCGC 2 cut(s) 160, 524
AclWI GGATC 2 cut(s) 313, 727
AcsI RAATTY 2 cut(s) 379, 448
AcuI CTGAAG 2 cut(s) 596, 651
AfaI GTAC 6 cut(s) 65, 82, 104, 164, 347, 584
AfiI CCNNNNNNNGG 2 cut(s) 427, 707
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 367, 667
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 4 cut(s) 265, 553, 610, 620
AluI AGCT 4 cut(s) 265, 553, 610, 620
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 2 cut(s) 313, 727
AoxI GGCC 1 cut(s) 497
ApeKI GCWGC 2 cut(s) 125, 553
ApoI RAATTY 2 cut(s) 379, 448
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp700I GAANNNNTTC 2 cut(s) 199, 671
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 308
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 537
AvaII GGWCC 1 cut(s) 308
BaeI ACNNNNGTAYC 4 cut(s) 72, 105, 154, 187
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 112, 540
BccI CCATC 3 cut(s) 104, 115, 287
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 87
BfuI GTATCC 1 cut(s) 674
BisI GCNGC 2 cut(s) 126, 554
BlsI GCNGC 2 cut(s) 127, 555
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 49, 309
BmrFI CCNGG 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 83, 550
BsaJI CCNNGG 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 707
Bse1I ACTGG 2 cut(s) 82, 478
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 391, 421
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 2 cut(s) 427, 707
BseNI ACTGG 2 cut(s) 82, 478
BseXI GCAGC 2 cut(s) 112, 540
BshFI GGCC 1 cut(s) 499
BsiHKAI GWGCWC 1 cut(s) 267
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 427, 707
BsmFI GGGAC 1 cut(s) 321
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 499
Bsp119I TTCGAA 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 3 cut(s) 318, 624, 719
BspACI CCGC 2 cut(s) 160, 524
BspANI GGCC 1 cut(s) 499
BspLI GGNNCC 2 cut(s) 49, 309
BspPI GGATC 2 cut(s) 313, 727
BspT104I TTCGAA 1 cut(s) 537
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 2 cut(s) 82, 478
BssECI CCNNGG 2 cut(s) 391, 421
BssMI GATC 3 cut(s) 318, 624, 719
BssSI CACGAG 1 cut(s) 336
BssT1I CCWWGG 2 cut(s) 391, 421
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 3 cut(s) 33, 375, 587
Bst6I CTCTTC 2 cut(s) 210, 627
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 522, 569
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 1 cut(s) 295
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 3 cut(s) 321, 627, 722
BstMBI GATC 3 cut(s) 318, 624, 719
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 286, 571
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 112, 540
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 1 cut(s) 499
BtsCI GGATG 1 cut(s) 295
BtsIMutI CAGTG 3 cut(s) 75, 371, 627
Cac8I GCNNGC 2 cut(s) 522, 569
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 308
Csp6I GTAC 6 cut(s) 64, 81, 103, 163, 346, 583
CviAII CATG 5 cut(s) 181, 283, 464, 568, 723
CviQI GTAC 6 cut(s) 64, 81, 103, 163, 346, 583
DdeI CTNAG 1 cut(s) 621
DpnI GATC 3 cut(s) 320, 626, 721
DpnII GATC 3 cut(s) 318, 624, 719
Eam1104I CTCTTC 2 cut(s) 210, 627
EarI CTCTTC 2 cut(s) 210, 627
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 391, 421
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 596, 651
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 308
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 391, 421
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 391, 421
FaeI CATG 5 cut(s) 184, 286, 467, 571, 726
FaqI GGGAC 1 cut(s) 321
FatI CATG 5 cut(s) 180, 282, 463, 567, 722
FauNDI CATATG 2 cut(s) 70, 145
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 2 cut(s) 126, 554
FokI GGATG 1 cut(s) 302
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 2 cut(s) 126, 554
GlaI GCGC 1 cut(s) 214
GluI GCNGC 2 cut(s) 126, 554
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 1 cut(s) 499
HhaI GCGC 1 cut(s) 215
Hin1II CATG 5 cut(s) 184, 286, 467, 571, 726
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 3 cut(s) 5, 334, 484
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 2 cut(s) 153, 604
Hpy188I TCNGA 2 cut(s) 24, 615
Hpy188III TCNNGA 2 cut(s) 257, 509
Hpy8I GTNNAC 2 cut(s) 153, 604
HpyAV CCTTC 4 cut(s) 38, 536, 587, 710
HpyCH4III ACNGT 3 cut(s) 33, 375, 587
HpyCH4V TGCA 3 cut(s) 36, 494, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 621
Hsp92II CATG 5 cut(s) 184, 286, 467, 571, 726
HspAI GCGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 318, 624, 719
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 2 cut(s) 112, 540
MaeIII GTNAC 3 cut(s) 73, 356, 640
MalI GATC 3 cut(s) 320, 626, 721
MboI GATC 3 cut(s) 318, 624, 719
MboII GAAGA 6 cut(s) 197, 415, 497, 551, 644, 679
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 2 cut(s) 379, 448
MlyI GAGTC 2 cut(s) 14, 328
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 3 cut(s) 131, 448, 628
MroXI GAANNNNTTC 2 cut(s) 199, 671
MseI TTAA 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeI CATATG 2 cut(s) 70, 145
NdeII GATC 3 cut(s) 318, 624, 719
NlaIII CATG 5 cut(s) 184, 286, 467, 571, 726
NlaIV GGNNCC 2 cut(s) 49, 309
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 2 cut(s) 286, 571
NspV TTCGAA 1 cut(s) 537
PaeI GCATGC 1 cut(s) 571
PceI AGGCCT 1 cut(s) 499
PciI ACATGT 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 2 cut(s) 199, 671
PfeI GAWTC 1 cut(s) 484
PflMI CCANNNNNTGG 1 cut(s) 707
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 2 cut(s) 127, 555
PleI GAGTC 2 cut(s) 13, 328
PpsI GAGTC 2 cut(s) 13, 328
PpuMI RGGWCCY 1 cut(s) 308
PscI ACATGT 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 49, 309
PspPI GGNCC 1 cut(s) 308
PspPPI RGGWCCY 1 cut(s) 308
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 6 cut(s) 65, 82, 104, 164, 347, 584
RsaNI GTAC 6 cut(s) 64, 81, 103, 163, 346, 583
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 2 cut(s) 126, 554
Sau3AI GATC 3 cut(s) 318, 624, 719
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 2 cut(s) 14, 328
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 267
SetI ASST 8 cut(s) 267, 313, 423, 492, 555, 596, 612, 622
SfcI CTRYAG 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 537
SinI GGWCC 1 cut(s) 308
SmlI CTYRAG 2 cut(s) 98, 529
SmoI CTYRAG 2 cut(s) 98, 529
SphI GCATGC 1 cut(s) 571
Sse9I AATT 2 cut(s) 379, 448
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 2 cut(s) 160, 524
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 2 cut(s) 391, 421
TaaI ACNGT 3 cut(s) 33, 375, 587
TaqI TCGA 2 cut(s) 8, 537
TasI AATT 2 cut(s) 379, 448
TatI WGTACW 2 cut(s) 102, 345
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 3 cut(s) 82, 378, 634
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 125, 553
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 3 cut(s) 82, 378, 634
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 2 cut(s) 379, 448
XceI RCATGY 2 cut(s) 286, 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 199, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.