Rh1BG167500

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002350
Physical Location & Seq
Forward (+)
26854499 .. 26855336
838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG167500.1

Sequence Viewer

Length: 744 bp
ATGAACTCAATGGACTCGAACCAGGCGAAATCCAGCCTACTGTGTTCCCCAGAAGCCTCTCAGTCTCAGAGAACACCGAACCCTTACGTGACTGGCACGTCTGTTGTGGCTCTCAAATACAAAGATGGAGTTTTGATGGCTGCTGATATGGGAGGTTCCTATGGATCGACCCTCCGATACAAGAGCATCGAACGAATGAAGCCTGTTGGAAAGCATTCTCTTCTCGGCGCAAGTGGAGAAATCAGTGATTTCCAGGAGCTGCTACGTTATCTTGACGAGCTCACCCAACAGGACAGCATGTGTGACGATGGAAACTCTTTGGGACCTAAAGAGATCCACAGCTACTTGACCCGTGTGATGTACAACAGGCGTAACAAGTTTGATCCGCTGTGGAACTCGCTTGTGCTTGGTGGAGTGAAGAACGGTAAGACATACCTCGGAATCGTTACTATGATAGGTTTGAACTTTGAGGACAATCATGTCGCTACCGGATTTGGAAACCACTTTGCAAGGCCTATTCTTCGTGATGAATGGCGCAAGGACTTGAGTTTCGAAGAAGGTGTTAGGCTTCTGGAGAAATGCATGCGTGTCCTTTTGTATCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAACATCACTAAAGAAGGTGTAACAATCTCTCAGCCCTACTCCTTGAAGACTTCCTGGAATTTCTCTGCTTTCCAAAATCCAACAGCAGGTGCTCAAGGATCATGGTAA

Protein Analysis

247

Amino Acids

27.59

Weight (kDa)

8.87

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 31 - 212 4.2e-33 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 713
AasI GACNNNNNNGTC 2 cut(s) 97, 479
Acc36I ACCTGC 1 cut(s) 713
AciI CCGC 1 cut(s) 386
AclWI GGATC 4 cut(s) 172, 328, 377, 742
AcsI RAATTY 1 cut(s) 694
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 2 cut(s) 463, 682
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 3 cut(s) 21, 252, 689
AluBI AGCT 5 cut(s) 259, 280, 342, 625, 635
AluI AGCT 5 cut(s) 259, 280, 342, 625, 635
Alw21I GWGCWC 2 cut(s) 282, 730
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 4 cut(s) 172, 328, 377, 742
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 1 cut(s) 512
ApeKI GCWGC 2 cut(s) 140, 259
ApoI RAATTY 1 cut(s) 694
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
Asp700I GAANNNNTTC 1 cut(s) 214
AspLEI GCGC 2 cut(s) 230, 537
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 552
AvaII GGWCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 689
Bbv12I GWGCWC 2 cut(s) 282, 730
BbvI GCAGC 2 cut(s) 127, 246
BccI CCATC 3 cut(s) 119, 130, 302
BciT130I CCWGG 3 cut(s) 23, 254, 691
BcoDI GTCTC 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 713
BisI GCNGC 2 cut(s) 141, 260
BlsI GCNGC 2 cut(s) 142, 261
Bme1390I CCNGG 3 cut(s) 23, 254, 691
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 157, 324
BmrFI CCNGG 3 cut(s) 23, 254, 691
BmsI GCATC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 689
BpmI CTGGAG 1 cut(s) 593
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 565, 714
BsaAI YACGTR 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 488
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 3 cut(s) 23, 254, 691
BseDI CCNNGG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 3 cut(s) 74, 80, 680
BseNI ACTGG 1 cut(s) 97
BseXI GCAGC 2 cut(s) 127, 246
BshFI GGCC 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 282, 730
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 514
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 282, 730
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 4 cut(s) 164, 333, 382, 734
BspACI CCGC 1 cut(s) 386
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 3 cut(s) 73, 79, 679
BspLI GGNNCC 2 cut(s) 157, 324
BspMI ACCTGC 1 cut(s) 713
BspPI GGATC 4 cut(s) 172, 328, 377, 742
BspT104I TTCGAA 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 4 cut(s) 164, 333, 382, 734
Bst2UI CCWGG 3 cut(s) 23, 254, 691
Bst4CI ACNGT 2 cut(s) 42, 425
Bst6I CTCTTC 1 cut(s) 225
BstAUI TGTACA 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 1 cut(s) 584
BstDEI CTNAG 3 cut(s) 60, 66, 666
BstHHI GCGC 2 cut(s) 230, 537
BstKTI GATC 4 cut(s) 167, 336, 385, 737
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 4 cut(s) 164, 333, 382, 734
BstNI CCWGG 3 cut(s) 23, 254, 691
BstNSI RCATGY 2 cut(s) 301, 586
BstSCI CCNGG 3 cut(s) 21, 252, 689
BstV1I GCAGC 2 cut(s) 127, 246
BstV2I GAAGAC 1 cut(s) 689
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 514
BtrI CACGTC 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 250
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 1 cut(s) 584
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 2 cut(s) 230, 537
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 361
CviAII CATG 4 cut(s) 298, 479, 583, 738
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 3 cut(s) 60, 66, 666
DpnI GATC 4 cut(s) 166, 335, 384, 736
DpnII GATC 4 cut(s) 164, 333, 382, 734
DrdI GACNNNNNNGTC 2 cut(s) 97, 479
DseDI GACNNNNNNGTC 2 cut(s) 97, 479
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 611
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 21, 252, 689
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 282
FaeI CATG 4 cut(s) 301, 482, 586, 741
FaiI YATR 8 cut(s) 149, 162, 299, 433, 452, 480, 584, 739
FaqI GGGAC 1 cut(s) 336
FatI CATG 4 cut(s) 297, 478, 582, 737
Fnu4HI GCNGC 2 cut(s) 141, 260
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 141, 260
GlaI GCGC 2 cut(s) 229, 536
GluI GCNGC 2 cut(s) 141, 260
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 1 cut(s) 514
HapII CCGG 1 cut(s) 489
HhaI GCGC 2 cut(s) 230, 537
Hin1II CATG 4 cut(s) 301, 482, 586, 741
Hin6I GCGC 2 cut(s) 228, 535
HinP1I GCGC 2 cut(s) 228, 535
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 2 cut(s) 14, 441
HpaII CCGG 1 cut(s) 489
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 619
Hpy188I TCNGA 4 cut(s) 69, 176, 440, 630
Hpy188III TCNNGA 4 cut(s) 272, 524, 572, 602
Hpy8I GTNNAC 1 cut(s) 619
HpyAV CCTTC 2 cut(s) 551, 644
HpyCH4III ACNGT 2 cut(s) 42, 425
HpyCH4IV ACGT 3 cut(s) 87, 98, 265
HpyCH4V TGCA 2 cut(s) 509, 582
HpyF3I CTNAG 3 cut(s) 60, 66, 666
HpySE526I ACGT 3 cut(s) 87, 98, 265
Hsp92II CATG 4 cut(s) 301, 482, 586, 741
HspAI GCGC 2 cut(s) 228, 535
Kzo9I GATC 4 cut(s) 164, 333, 382, 734
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 127, 246
LweI GCATC 1 cut(s) 195
MaeII ACGT 3 cut(s) 87, 98, 265
MaeIII GTNAC 5 cut(s) 88, 302, 371, 445, 655
MalI GATC 4 cut(s) 166, 335, 384, 736
MboI GATC 4 cut(s) 164, 333, 382, 734
MboII GAAGA 5 cut(s) 212, 430, 512, 566, 694
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 282, 730
MluCI AATT 1 cut(s) 694
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 187, 740
MnlI CCTC 5 cut(s) 67, 146, 182, 446, 463
Mph1103I ATGCAT 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 3 cut(s) 23, 254, 691
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 3 cut(s) 23, 254, 691
NdeII GATC 4 cut(s) 164, 333, 382, 734
NlaIII CATG 4 cut(s) 301, 482, 586, 741
NlaIV GGNNCC 2 cut(s) 157, 324
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 88, 302
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 2 cut(s) 301, 586
NspV TTCGAA 1 cut(s) 552
PaeI GCATGC 1 cut(s) 586
PaqCI CACCTGC 1 cut(s) 713
PceI AGGCCT 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 441
PfoI TCCNGGA 2 cut(s) 252, 689
PkrI GCNGC 2 cut(s) 142, 261
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 21, 252, 689
PspGI CCWGG 3 cut(s) 21, 252, 689
PspN4I GGNNCC 2 cut(s) 157, 324
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SacI GAGCTC 1 cut(s) 282
SatI GCNGC 2 cut(s) 141, 260
Sau3AI GATC 4 cut(s) 164, 333, 382, 734
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 3 cut(s) 23, 254, 691
SduI GDGCHC 2 cut(s) 282, 730
SfaNI GCATC 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 552
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 544, 729
SmoI CTYRAG 2 cut(s) 544, 729
SphI GCATGC 1 cut(s) 586
Sse9I AATT 1 cut(s) 694
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 1 cut(s) 386
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 514
StyD4I CCNGG 3 cut(s) 21, 252, 689
TaaI ACNGT 2 cut(s) 42, 425
TaiI ACGT 3 cut(s) 90, 101, 268
TaqI TCGA 4 cut(s) 17, 167, 189, 552
TasI AATT 1 cut(s) 694
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 1 cut(s) 441
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 88, 302
TseI GCWGC 2 cut(s) 140, 259
Tsp45I GTSAC 2 cut(s) 88, 302
TspDTI ATGAA 3 cut(s) 17, 212, 543
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 694
XceI RCATGY 2 cut(s) 301, 586
XmnI GAANNNNTTC 1 cut(s) 214
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.