Rh5DG421000

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002350
Physical Location & Seq
Forward (+)
67045678 .. 67049686
4009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG421000.1

Sequence Viewer

Length: 609 bp
ATGGCTGCTGATATGGGAGGTTCATATGGATCTACCCTGCGGTACAAGAGTGTGGAACGAATGAAGCCTATTGGAAAACATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGATTTCCAGGAGTTGTTAAAATATCTTGATGAGCTTGTCCTATATGACAACATGTGGGATGATGGGAACTCTTTGGGACCTAAAGAGATCCACAACTATTTGACCCGTGTGATGTACAATCGCCGTAACAAGTTCAACCCACTGTGGAATTCACTTGTCCTTGGTGGAGTGAAAAACGGAGAGAAATACCTTGGAATGGTTAGTATGATAGGTGTAAATTTTGAGGACAACCATGTTGCTACCGGATTTGGGAATCACCTTGCAAGGCCTATTCTTCGTGATGAGTGGCGTGAGGACTTGAGTTTCGAAGAAGGCGTGAAGCTGCTGGAGAAGTGCATGCGTGTCCTTCTGTACCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAAGATCACTGGAGAAGGTGTAACAATCTCTCAACCCTACTCTTTGAAGACTTTCTGGGGATTCTCTGCTTTCCAAAATCCAACTGCCGGTGCTGAAGGATCATGGTAA

Protein Analysis

202

Amino Acids

22.84

Weight (kDa)

6.92

Isoelectric Point (pI)

28.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 1 - 169 4.4e-25 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 40
AclWI GGATC 3 cut(s) 37, 193, 607
AcsI RAATTY 2 cut(s) 259, 328
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 3 cut(s) 44, 227, 464
AfiI CCNNNNNNNGG 3 cut(s) 307, 360, 587
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 2 cut(s) 247, 547
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 4 cut(s) 145, 433, 490, 500
AluI AGCT 4 cut(s) 145, 433, 490, 500
AlwI GGATC 3 cut(s) 37, 193, 607
AoxI GGCC 1 cut(s) 377
ApeKI GCWGC 2 cut(s) 5, 433
ApoI RAATTY 2 cut(s) 259, 328
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
Asp700I GAANNNNTTC 2 cut(s) 79, 551
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 417
AvaII GGWCC 1 cut(s) 188
BaeI ACNNNNGTAYC 2 cut(s) 34, 67
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 1 cut(s) 420
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 1 cut(s) 119
BisI GCNGC 2 cut(s) 6, 434
BlsI GCNGC 2 cut(s) 7, 435
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 2 cut(s) 458, 531
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 430
BsaJI CCNNGG 2 cut(s) 271, 301
BsaWI WCCGGW 1 cut(s) 353
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 360, 587
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 271, 301
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 3 cut(s) 307, 360, 587
BseNI ACTGG 1 cut(s) 514
BseXI GCAGC 1 cut(s) 420
BshFI GGCC 1 cut(s) 379
BsiSI CCGG 2 cut(s) 354, 588
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 3 cut(s) 307, 360, 587
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 417
Bsp1407I TGTACA 1 cut(s) 225
Bsp143I GATC 4 cut(s) 29, 198, 504, 599
BspACI CCGC 1 cut(s) 40
BspANI GGCC 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 3 cut(s) 37, 193, 607
BspT104I TTCGAA 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 587
BsrGI TGTACA 1 cut(s) 225
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 587
BssECI CCNNGG 2 cut(s) 271, 301
BssMI GATC 4 cut(s) 29, 198, 504, 599
BssT1I CCWWGG 2 cut(s) 271, 301
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 2 cut(s) 255, 467
Bst6I CTCTTC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 225
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 4 cut(s) 32, 201, 507, 602
BstMBI GATC 4 cut(s) 29, 198, 504, 599
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 166, 451
BstSCI CCNGG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 420
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 2 cut(s) 29, 198
BstYI RGATCY 2 cut(s) 29, 198
BsuRI GGCC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 251, 507
Cac8I GCNNGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 587
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 43, 226, 463
CviAII CATG 4 cut(s) 163, 344, 448, 603
CviJI RGCY 7 cut(s) 5, 67, 145, 379, 433, 490, 500
CviKI_1 RGCY 7 cut(s) 5, 67, 145, 379, 433, 490, 500
CviQI GTAC 3 cut(s) 43, 226, 463
DdeI CTNAG 1 cut(s) 501
DpnI GATC 4 cut(s) 31, 200, 506, 601
DpnII GATC 4 cut(s) 29, 198, 504, 599
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 271, 301
Eco147I AGGCCT 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 476
EcoO109I RGGNCCY 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 271, 301
ErhI CCWWGG 2 cut(s) 271, 301
FaeI CATG 4 cut(s) 166, 347, 451, 606
FaqI GGGAC 1 cut(s) 201
FatI CATG 4 cut(s) 162, 343, 447, 602
FauNDI CATATG 1 cut(s) 25
Fnu4HI GCNGC 2 cut(s) 6, 434
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 6, 434
GluI GCNGC 2 cut(s) 6, 434
GsuI CTGGAG 2 cut(s) 458, 531
HaeIII GGCC 1 cut(s) 379
HapII CCGG 2 cut(s) 354, 588
Hin1II CATG 4 cut(s) 166, 347, 451, 606
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 364, 561
HpaII CCGG 2 cut(s) 354, 588
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 137, 389
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 4 cut(s) 416, 467, 509, 590
HpyCH4III ACNGT 2 cut(s) 255, 467
HpyCH4V TGCA 3 cut(s) 95, 374, 447
HpyF3I CTNAG 1 cut(s) 501
Hsp92II CATG 4 cut(s) 166, 347, 451, 606
Kzo9I GATC 4 cut(s) 29, 198, 504, 599
LpnPI CCDG 8 cut(s) 50, 104, 131, 367, 422, 495, 541, 601
Lsp1109I GCAGC 1 cut(s) 420
MaeIII GTNAC 2 cut(s) 236, 520
MalI GATC 4 cut(s) 31, 200, 506, 601
MboI GATC 4 cut(s) 29, 198, 504, 599
MboII GAAGA 4 cut(s) 77, 377, 431, 559
MflI RGATCY 2 cut(s) 29, 198
MluCI AATT 2 cut(s) 259, 328
MmeI TCCRAC 1 cut(s) 605
MnlI CCTC 3 cut(s) 11, 328, 397
MroXI GAANNNNTTC 2 cut(s) 79, 551
MseI TTAA 1 cut(s) 128
MspI CCGG 2 cut(s) 354, 588
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
NdeI CATATG 1 cut(s) 25
NdeII GATC 4 cut(s) 29, 198, 504, 599
NlaIII CATG 4 cut(s) 166, 347, 451, 606
NlaIV GGNNCC 1 cut(s) 189
NspI RCATGY 2 cut(s) 166, 451
NspV TTCGAA 1 cut(s) 417
PaeI GCATGC 1 cut(s) 451
PceI AGGCCT 1 cut(s) 379
PciI ACATGT 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PdmI GAANNNNTTC 2 cut(s) 79, 551
PfeI GAWTC 2 cut(s) 364, 561
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 2 cut(s) 7, 435
PpuMI RGGWCCY 1 cut(s) 188
PscI ACATGT 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 188
PspPPI RGGWCCY 1 cut(s) 188
PsuI RGATCY 2 cut(s) 29, 198
RsaI GTAC 3 cut(s) 44, 227, 464
RsaNI GTAC 3 cut(s) 43, 226, 463
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 6, 434
Sau3AI GATC 4 cut(s) 29, 198, 504, 599
Sau96I GGNCC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
SphI GCATGC 1 cut(s) 451
Sse9I AATT 2 cut(s) 259, 328
SseBI AGGCCT 1 cut(s) 379
SsiI CCGC 1 cut(s) 40
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 271, 301
TaaI ACNGT 2 cut(s) 255, 467
TaqI TCGA 1 cut(s) 417
TasI AATT 2 cut(s) 259, 328
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 2 cut(s) 364, 561
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 2 cut(s) 258, 514
TseI GCWGC 2 cut(s) 5, 433
TspDTI ATGAA 2 cut(s) 12, 77
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 2 cut(s) 258, 514
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 2 cut(s) 259, 328
XceI RCATGY 2 cut(s) 166, 451
XmnI GAANNNNTTC 2 cut(s) 79, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.