Rw1G016880

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002350
Physical Location & Seq
Forward (+)
37484616 .. 37485350
735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016880.1

Sequence Viewer

Length: 735 bp
ATGGACTCGAACCAGGCGAAACCCAGCCTACTGTATTCCCCAGAAGCCTCTCAGTCTCAGAGAACACCGAACCCTTACGTGACTGGCACGTCAGTTGTGGCTCTCAAATACAAAGATGGAGTTTTGATGGCTGCTGATATGGGAGGTTCTTATGGATCGACCCTCCGATACAAAAGCATCGAAAGAATGAAGCCTATTGGAAAGCATTCTCTTCTCGGCGCAAGTGGAGAAATCAGTGATTTCCAGGAGCTGCTACGTTATCTTGACGAGCTCACCCAACAGGACAGCATGTGTGATGATGGAACCTCTTTGGGTCCTAAAGAGATCCACAGCTACTTGACCCGTGTGATGTATGACAGGCGTAACAAGTTTGATCCGCTGTGGAACTCGATTGTGCTTGGTGGAGTGAAGAACGGTAAGACATACCTCGGAATGGTTACTATGATAGGTTTGAACTTTGAGGACAATCATGTCGCTACCGGATTTGGAAACCACCTTGCAAGGCCTATTCTTCGTGATGAATGGCGCAAGGACTTGAGTTTCGAAGAAGGTGTTAGGCTTCTTGAGAAATGCATGCGTGTCCTTTTGTATCGTGATAGGTCTGCTGTCAACAAGCTTCAGATAGCTAACATCACTAAAGAAGGTGTAACAATCTCTCAGCCCTACTCCTTGAAGACTTCCTGGAATTTCTCTGCTTTCCAAAATCCAACAGCAGGTGCTCAAGGATCATGGTAA

Protein Analysis

244

Amino Acids

27.32

Weight (kDa)

8.73

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 28 - 209 4.6e-33 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 704
AasI GACNNNNNNGTC 2 cut(s) 88, 470
Acc36I ACCTGC 1 cut(s) 704
AciI CCGC 1 cut(s) 377
AclWI GGATC 4 cut(s) 163, 319, 368, 733
AcsI RAATTY 1 cut(s) 685
AcuI CTGAAG 1 cut(s) 602
AfiI CCNNNNNNNGG 2 cut(s) 433, 713
AgsI TTSAA 2 cut(s) 454, 673
AjiI CACGTC 1 cut(s) 90
AjnI CCWGG 3 cut(s) 12, 243, 680
AluBI AGCT 5 cut(s) 250, 271, 333, 616, 626
AluI AGCT 5 cut(s) 250, 271, 333, 616, 626
Alw21I GWGCWC 2 cut(s) 273, 721
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 4 cut(s) 163, 319, 368, 733
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 131, 250
ApoI RAATTY 1 cut(s) 685
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 1 cut(s) 205
AspLEI GCGC 2 cut(s) 221, 528
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 265
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 2 cut(s) 273, 721
BbvI GCAGC 2 cut(s) 118, 237
BccI CCATC 3 cut(s) 110, 121, 293
BciT130I CCWGG 3 cut(s) 14, 245, 682
BcoDI GTCTC 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 704
BisI GCNGC 2 cut(s) 132, 251
BlsI GCNGC 2 cut(s) 133, 252
Bme1390I CCNGG 3 cut(s) 14, 245, 682
Bme18I GGWCC 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 2 cut(s) 304, 315
BmrFI CCNGG 3 cut(s) 14, 245, 682
BmsI GCATC 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 543
BpuEI CTTGAG 3 cut(s) 556, 584, 705
BsaAI YACGTR 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 713
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 3 cut(s) 14, 245, 682
BseDI CCNNGG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 433, 713
BseMII CTCAG 3 cut(s) 65, 71, 671
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 2 cut(s) 118, 237
BseYI CCCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 505
BsiHKAI GWGCWC 2 cut(s) 273, 721
BsiSI CCGG 1 cut(s) 480
BslI CCNNNNNNNGG 2 cut(s) 433, 713
BsmAI GTCTC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 1 cut(s) 505
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 2 cut(s) 273, 721
Bsp143I GATC 4 cut(s) 155, 324, 373, 725
BspACI CCGC 1 cut(s) 377
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 3 cut(s) 64, 70, 670
BspLI GGNNCC 2 cut(s) 304, 315
BspMI ACCTGC 1 cut(s) 704
BspPI GGATC 4 cut(s) 163, 319, 368, 733
BspT104I TTCGAA 1 cut(s) 543
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 4 cut(s) 155, 324, 373, 725
Bst2UI CCWGG 3 cut(s) 14, 245, 682
Bst4CI ACNGT 2 cut(s) 33, 416
Bst6I CTCTTC 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 79
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 3 cut(s) 51, 57, 657
BstHHI GCGC 2 cut(s) 221, 528
BstKTI GATC 4 cut(s) 158, 327, 376, 728
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 4 cut(s) 155, 324, 373, 725
BstNI CCWGG 3 cut(s) 14, 245, 682
BstNSI RCATGY 2 cut(s) 292, 577
BstSCI CCNGG 3 cut(s) 12, 243, 680
BstV1I GCAGC 2 cut(s) 118, 237
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 1 cut(s) 505
BtrI CACGTC 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 241
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 1 cut(s) 575
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 2 cut(s) 221, 528
Cfr13I GGNCC 1 cut(s) 314
CviAII CATG 4 cut(s) 289, 470, 574, 729
DdeI CTNAG 3 cut(s) 51, 57, 657
DpnI GATC 4 cut(s) 157, 326, 375, 727
DpnII GATC 4 cut(s) 155, 324, 373, 725
DrdI GACNNNNNNGTC 2 cut(s) 88, 470
DseDI GACNNNNNNGTC 2 cut(s) 88, 470
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 505
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 314
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 12, 243, 680
EcoT22I ATGCAT 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 273
FaeI CATG 4 cut(s) 292, 473, 577, 732
FaiI YATR 9 cut(s) 140, 153, 290, 354, 424, 443, 471, 575, 730
FatI CATG 4 cut(s) 288, 469, 573, 728
Fnu4HI GCNGC 2 cut(s) 132, 251
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 2 cut(s) 132, 251
GlaI GCGC 2 cut(s) 220, 527
GluI GCNGC 2 cut(s) 132, 251
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 505
HapII CCGG 1 cut(s) 480
HhaI GCGC 2 cut(s) 221, 528
Hin1II CATG 4 cut(s) 292, 473, 577, 732
Hin6I GCGC 2 cut(s) 219, 526
HinP1I GCGC 2 cut(s) 219, 526
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 480
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 1 cut(s) 610
Hpy188I TCNGA 4 cut(s) 60, 167, 431, 621
Hpy188III TCNNGA 4 cut(s) 263, 515, 563, 593
Hpy8I GTNNAC 1 cut(s) 610
HpyAV CCTTC 2 cut(s) 542, 635
HpyCH4III ACNGT 2 cut(s) 33, 416
HpyCH4IV ACGT 3 cut(s) 78, 89, 256
HpyCH4V TGCA 2 cut(s) 500, 573
HpyF3I CTNAG 3 cut(s) 51, 57, 657
HpySE526I ACGT 3 cut(s) 78, 89, 256
Hsp92II CATG 4 cut(s) 292, 473, 577, 732
HspAI GCGC 2 cut(s) 219, 526
Kzo9I GATC 4 cut(s) 155, 324, 373, 725
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 118, 237
LweI GCATC 1 cut(s) 186
MaeII ACGT 3 cut(s) 78, 89, 256
MaeIII GTNAC 4 cut(s) 79, 362, 436, 646
MalI GATC 4 cut(s) 157, 326, 375, 727
MboI GATC 4 cut(s) 155, 324, 373, 725
MboII GAAGA 5 cut(s) 203, 421, 503, 557, 685
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 2 cut(s) 273, 721
MluCI AATT 1 cut(s) 685
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 6 cut(s) 58, 137, 173, 316, 437, 454
Mph1103I ATGCAT 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 205
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 3 cut(s) 14, 245, 682
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 3 cut(s) 14, 245, 682
NdeII GATC 4 cut(s) 155, 324, 373, 725
NlaIII CATG 4 cut(s) 292, 473, 577, 732
NlaIV GGNNCC 2 cut(s) 304, 315
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 575
NspI RCATGY 2 cut(s) 292, 577
NspV TTCGAA 1 cut(s) 543
PaeI GCATGC 1 cut(s) 577
PaqCI CACCTGC 1 cut(s) 704
PceI AGGCCT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 205
PfoI TCCNGGA 2 cut(s) 243, 680
PkrI GCNGC 2 cut(s) 133, 252
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 3 cut(s) 12, 243, 680
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 3 cut(s) 12, 243, 680
PspN4I GGNNCC 2 cut(s) 304, 315
PspPI GGNCC 1 cut(s) 314
PspPPI RGGWCCY 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 324
SacI GAGCTC 1 cut(s) 273
SatI GCNGC 2 cut(s) 132, 251
Sau3AI GATC 4 cut(s) 155, 324, 373, 725
Sau96I GGNCC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 14, 245, 682
SduI GDGCHC 2 cut(s) 273, 721
SfaNI GCATC 1 cut(s) 186
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 314
SmlI CTYRAG 3 cut(s) 535, 563, 720
SmoI CTYRAG 3 cut(s) 535, 563, 720
SphI GCATGC 1 cut(s) 577
Sse9I AATT 1 cut(s) 685
SseBI AGGCCT 1 cut(s) 505
SsiI CCGC 1 cut(s) 377
SstI GAGCTC 1 cut(s) 273
StuI AGGCCT 1 cut(s) 505
StyD4I CCNGG 3 cut(s) 12, 243, 680
TaaI ACNGT 2 cut(s) 33, 416
TaiI ACGT 3 cut(s) 81, 92, 259
TaqI TCGA 5 cut(s) 8, 158, 180, 389, 543
TasI AATT 1 cut(s) 685
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 2 cut(s) 131, 250
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 203, 534
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 1 cut(s) 685
XceI RCATGY 2 cut(s) 292, 577
XmnI GAANNNNTTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.