AT1G67550
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002839
Physical Location & Seq
Forward (+)
25312660 .. 25317495
4836 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67550.1

Sequence Viewer

Length: 2517 bp
ATGAAGTTGTTGCCGCGAGAGATCGAGAAGTTAGAGCTACATCAAGCTGGTTTCCTTGCACAGAAACGTCTTGCTCGTGGTATTAGACTCAATTACACTGAAGCTGTAGCACTTATTGCTACTCAGATTCTTGAGTTTATTCGTGATGGTGATAAAAGCGTGGCTGAGCTAATGGATATCGGGAGACAACTATTAGGAAGGAGACAGGTTCTTCCTGCTGTTTTACATCTTTTGTATACGGTTCAGGTCGAAGGTACATTTCGTGATGGTACAAAGTTAGTCACGGTTCATGAGCCGATTTCTCTTGAAAACGGCAACCTTGAACTTGCTTTGCATGGATCTTTCCTTCCAGTTCCTTCATTGGATAAATTTCCAGAAGTTCATGAGGGTGTAATTATTCCTGGTGATATGAAATATGGAGATGGAAGTATAATTATAAATCATGGAAGGAAAGCAGTAGTTCTGAAAGTAGTGAACACTGGAGACAGACCAGTGCAGGTTGGAAGTCACTACCATTTTATTGAAGTGAATCCACTATTGGTGTTTGACAGACGTAAAGCTCTTGGTATGCGTTTAAACATACCCGCTGGAACAGCTGTTCGCTTTGAGCCAGGAGAAAGAAAAAGCGTTGTACTTGTGAATATTGGTGGAAATAAAGTGATAAGGGGAGGAAATGGAATTGTTGATGGTCTAGTTGATGATGTCAATTGGACAGTTTTAATGGAAACAATGGAGAGAAGAGGATTTAAGCACCTGGAAGATATTGATGCTAGTGAGGGGATAGCAGGGGAAGATCCTCGATTTACAACTATGATTTCTCGTGAAAAGTATGCAAATATGTATGGTCCTACCACTGGGGACAAGCTTCGCCTAGGTGATACCAACTTATACGCAAGAATTGAAAAAGATTATACTGTTTATGGAGATGAATGTGTATTTGGAGGTGGAAAAGTTCTAAGAGAAGGCATGGGACAAGGCATAGAGCAAGCTGAAGCTCTTTCTTTGGATACTGTCATTACCAATAGTGTGATAATTGATTATTCAGGAATATATAAGGCAGATATTGGCATTAAAAATGGTCATATTGTTGGTATTGGGAAAGCAGGCAATCCAGATACCATGCATGGTGTGCAGAACAATATGCTTATTGGGAATAAGACTGAGGTTATTGCTGGAGAAGGAATGATTGTAACTGCAGGAGCTATTGATTGTCATGTACATTTCATATGTCCTCAATTGGTGTATGAAGCAGTGTCAAGCGGCATTACAACTATGGTTGGGGGAGGGACAGGGCCTGCTTATGGTACACGTGCTACTACGTGTACCCCTTCTCCGTTCGATATGAAGTTAATGCTGCAGTCCACAGATAGCCTGCCACTAAACTTTGGCTTTACAGGAAAGGGAAATACAGCTAAACCGTTAGAGCTTCGTCACATAGTGGAGGCTGGAGCAATGGGATTGAAGCTGCATGAGGACTGGGGAACTACTCCTGCTGCAATAGACAATTGTTTGGCAGTTGCAGAAGAATATGATATCCAGGTGAACATTCATACTGACACCTTAAATGAATCCGGTTTTGTTGAGCACACTATCAATGCATTTCGTGGGAGAACAATACATACATATCACAGTGAAGGTGCTGGTGGTGGACATGCACCAGATATCATTAGAGTTTGTGGAGTGAAAAATGTACTCCCGTCATCAACCAACCCAACACGGCCATATACGAAAAATACTGTAGATGAACATCTTGACATGCTGATGGTTTGCCATCACCTTGACAAGAACATCCCAGAAGATGTAGCTTTTGCTGAATCAAGAATAAGAGCTGAAACAATAGCTGCAGAGGATATATTGCATGATATGGGAGCAATCAGCATTATCTCCTCCGACTCACAAGCAATGGGTCGCATTGGAGAGGTAATAAGCAGAACTTGGCAGACAGCTGACAAGATGAAGGCTCAGAGGGGAGCCATTGATCCTAACATGGCTGATGATGATAACTCAAGGATCAAGAGATACATTGCAAAGTACACTATAAACCCAGCTATAGCCAATGGGTTTGCAGACTTAATTGGCTCGGTTGAGGTGAAGAAGCTGGCTGATCTTGTTATATGGCAGCCGGCTTTCTTCGGAGCAAAACCAGAAATGATAATCAAAGGAGGCAATATAGCATGGGCTAATATGGGCGATGCAAATGCGAGCATACCCACTCCTGAGCCGGTGATATCAAGACCTATGTTTGGAGCATTTGGAAAGGCCGGAAGTGAAAACTCTGTTGCATTTGTCAGCAAGGCTGCCTTGAGAAAGGGGGTAAAAGAACTATATGGACTCAAGAAGAGGGTTGTAGCGGTGTCCAACGTGAGGCAGCTCACAAAACTCGACATGAAGCTGAATGATGCGCTTCCAGAGATCACCGTGGATCCAGAGACATACGTTGTCACAGCAAATGGCGAGGTCCTTACATGTGCGCCAGCCGATTCAGTCCCTCTCTCCCGGAACTATTTCCTCTTTTAA

Protein Analysis

838

Amino Acids

91.02

Weight (kDa)

5.92

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urease_gamma PF00547 1 - 100 8.1e-41 Urease, gamma subunit
Urease_beta PF00699 134 - 230 1e-41 Urease beta subunit
Urease_linker PF18473 232 - 265 2.8e-16 Urease subunit beta-alpha linker domain
Urease_alpha PF00449 271 - 390 9e-56 Urease alpha-subunit, N-terminal domain
Amidohydro_1 PF01979 396 - 722 7.5e-76 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 236
AccII CGCG 1 cut(s) 16
AciI CCGC 4 cut(s) 14, 585, 1260, 2351
AclWI GGATC 6 cut(s) 346, 788, 1973, 2018, 2417, 2430
AcoI YGGCCR 1 cut(s) 1718
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 2 cut(s) 120, 1011
AcvI CACGTG 1 cut(s) 1310
AdeI CACNNNGTG 1 cut(s) 1438
AfaI GTAC 8 cut(s) 256, 271, 633, 1218, 1306, 1324, 1692, 2033
AfiI CCNNNNNNNGG 4 cut(s) 854, 1301, 2243, 2364
AflIII ACRYGT 3 cut(s) 1307, 1319, 2465
AgsI TTSAA 5 cut(s) 308, 323, 524, 902, 1462
AjnI CCWGG 4 cut(s) 400, 610, 753, 1536
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 921, 953
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 1587
Alw26I GTCTC 4 cut(s) 178, 196, 477, 2423
AlwI GGATC 6 cut(s) 346, 788, 1973, 2018, 2417, 2430
AlwNI CAGNNNCTG 1 cut(s) 1295
AoxI GGCC 3 cut(s) 1292, 1718, 2259
ApeKI GCWGC 7 cut(s) 1354, 1465, 1493, 1841, 2119, 2297, 2368
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 2504
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 2 cut(s) 2404, 2473
AspS9I GGNCC 3 cut(s) 845, 1292, 2458
AsuC2I CCSGG 1 cut(s) 2497
AsuHPI GGTGA 8 cut(s) 161, 416, 887, 1552, 1766, 2101, 2236, 2407
AvaII GGWCC 2 cut(s) 845, 2458
AvrII CCTAGG 1 cut(s) 871
BamHI GGATCC 1 cut(s) 2422
BauI CACGAG 2 cut(s) 75, 819
BbrPI CACGTG 1 cut(s) 1310
Bbv12I GWGCWC 1 cut(s) 1587
BbvI GCAGC 7 cut(s) 1341, 1452, 1480, 1828, 2131, 2284, 2380
BccI CCATC 6 cut(s) 140, 260, 416, 680, 1756, 1779
BceAI ACGGC 2 cut(s) 328, 1733
BcgI CGANNNNNNTGC 2 cut(s) 2180, 2214
BciT130I CCWGG 4 cut(s) 402, 612, 755, 1538
BciVI GTATCC 1 cut(s) 1000
BcnI CCSGG 1 cut(s) 2497
BcoDI GTCTC 4 cut(s) 178, 196, 477, 2423
BfaI CTAG 3 cut(s) 692, 771, 872
BfmI CTRYAG 6 cut(s) 105, 1194, 1355, 1737, 1842, 2049
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 1 cut(s) 1000
BisI GCNGC 9 cut(s) 14, 1261, 1355, 1466, 1494, 1842, 2120, 2298, 2369
BlnI CCTAGG 1 cut(s) 871
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 9 cut(s) 15, 1262, 1356, 1467, 1495, 1843, 2121, 2299, 2370
Bme1390I CCNGG 5 cut(s) 402, 612, 755, 1538, 2497
Bme18I GGWCC 2 cut(s) 845, 2458
BmgT120I GGNCC 3 cut(s) 845, 1292, 2458
BmiI GGNNCC 2 cut(s) 1972, 2424
BmrFI CCNGG 5 cut(s) 402, 612, 755, 1538, 2497
BmrI ACTGGG 2 cut(s) 864, 1486
BmsI GCATC 3 cut(s) 757, 2182, 2389
BmuI ACTGGG 2 cut(s) 864, 1486
BpmI CTGGAG 3 cut(s) 501, 1194, 1467
Bpu10I CCTNAGC 1 cut(s) 2217
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 4 cut(s) 152, 1990, 2318, 2323
BpuMI CCSGG 1 cut(s) 2497
BsaAI YACGTR 2 cut(s) 1310, 1320
BsaBI GATNNNNATC 1 cut(s) 1746
BsaJI CCNNGG 2 cut(s) 871, 2418
BsaWI WCCGGW 1 cut(s) 1571
BsaXI ACNNNNNCTCC 2 cut(s) 1315, 1345
Bsc4I CCNNNNNNNGG 4 cut(s) 854, 1301, 2243, 2364
Bse118I RCCGGY 2 cut(s) 2122, 2221
Bse1I ACTGG 5 cut(s) 350, 484, 491, 859, 1481
Bse3DI GCAATG 3 cut(s) 1458, 1908, 2022
Bse8I GATNNNNATC 1 cut(s) 1746
BseBI CCWGG 4 cut(s) 402, 612, 755, 1538
BseDI CCNNGG 2 cut(s) 871, 2418
BseGI GGATG 1 cut(s) 1788
BseJI GATNNNNATC 1 cut(s) 1746
BseLI CCNNNNNNNGG 4 cut(s) 854, 1301, 2243, 2364
BseMI GCAATG 3 cut(s) 1458, 1908, 2022
BseMII CTCAG 5 cut(s) 137, 156, 1152, 1976, 2208
BseNI ACTGG 5 cut(s) 350, 484, 491, 859, 1481
BseRI GAGGAG 1 cut(s) 1876
BseXI GCAGC 7 cut(s) 1341, 1452, 1480, 1828, 2131, 2284, 2380
BseYI CCCAGC 1 cut(s) 2044
BsgI GTGCAG 2 cut(s) 515, 1151
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 3 cut(s) 1294, 1720, 2261
BsiHKAI GWGCWC 1 cut(s) 1587
BsiSI CCGG 5 cut(s) 1572, 2123, 2222, 2262, 2497
BslFI GGGAC 4 cut(s) 872, 984, 1300, 2471
BslI CCNNNNNNNGG 4 cut(s) 854, 1301, 2243, 2364
BsmAI GTCTC 4 cut(s) 178, 196, 477, 2423
BsmFI GGGAC 4 cut(s) 872, 984, 1300, 2471
BsnI GGCC 3 cut(s) 1294, 1720, 2261
Bsp1286I GDGCHC 1 cut(s) 1587
Bsp1407I TGTACA 1 cut(s) 1216
Bsp143I GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 4 cut(s) 14, 585, 1260, 2351
BspANI GGCC 3 cut(s) 1294, 1720, 2261
BspCNI CTCAG 5 cut(s) 136, 157, 1153, 1975, 2209
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 2 cut(s) 289, 382
BspLI GGNNCC 2 cut(s) 1972, 2424
BspMAI CTGCAG 3 cut(s) 1198, 1359, 1846
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 6 cut(s) 346, 788, 1973, 2018, 2417, 2430
BsrDI GCAATG 3 cut(s) 1458, 1908, 2022
BsrFI RCCGGY 2 cut(s) 2122, 2221
BsrGI TGTACA 1 cut(s) 1216
BsrI ACTGG 5 cut(s) 350, 484, 491, 859, 1481
BssAI RCCGGY 2 cut(s) 2122, 2221
BssECI CCNNGG 2 cut(s) 871, 2418
BssMI GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
BssNAI GTATAC 1 cut(s) 237
BssSI CACGAG 2 cut(s) 75, 819
BssT1I CCWWGG 1 cut(s) 871
Bst1107I GTATAC 1 cut(s) 237
Bst2BI CACGAG 2 cut(s) 75, 819
Bst2UI CCWGG 4 cut(s) 402, 612, 755, 1538
Bst4CI ACNGT 9 cut(s) 241, 286, 715, 916, 1012, 1419, 1631, 1738, 2419
Bst6I CTCTTC 2 cut(s) 733, 2333
BstAPI GCANNNNNTGC 2 cut(s) 116, 1129
BstAUI TGTACA 1 cut(s) 1216
BstBAI YACGTR 2 cut(s) 1310, 1320
BstC8I GCNNGC 8 cut(s) 987, 1105, 1296, 1373, 2100, 2124, 2203, 2475
BstDEI CTNAG 6 cut(s) 123, 165, 956, 1161, 1962, 2217
BstDSI CCRYGG 1 cut(s) 2418
BstF5I GGATG 1 cut(s) 1788
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 2 cut(s) 2404, 2473
BstKTI GATC 8 cut(s) 24, 341, 796, 1981, 2013, 2107, 2415, 2425
BstMAI GTCTC 4 cut(s) 178, 196, 477, 2423
BstMBI GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
BstMWI GCNNNNNNNGC 3 cut(s) 116, 593, 1129
BstNI CCWGG 4 cut(s) 402, 612, 755, 1538
BstNSI RCATGY 3 cut(s) 1655, 1759, 2469
BstSCI CCNGG 5 cut(s) 400, 610, 753, 1536, 2495
BstSFI CTRYAG 6 cut(s) 105, 1194, 1355, 1737, 1842, 2049
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 7 cut(s) 1341, 1452, 1480, 1828, 2131, 2284, 2380
BstX2I RGATCY 3 cut(s) 338, 793, 2422
BstYI RGATCY 3 cut(s) 338, 793, 2422
BstZ17I GTATAC 1 cut(s) 237
BsuI GTATCC 1 cut(s) 1000
BsuRI GGCC 3 cut(s) 1294, 1720, 2261
BtgI CCRYGG 1 cut(s) 2418
BtgZI GCGATG 1 cut(s) 2205
BtsCI GGATG 1 cut(s) 1788
BtsI GCAGTG 1 cut(s) 1257
BtsIMutI CAGTG 6 cut(s) 96, 477, 498, 852, 1257, 1636
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 8 cut(s) 987, 1105, 1296, 1373, 2100, 2124, 2203, 2475
CaiI CAGNNNCTG 1 cut(s) 1295
CciI TCATGA 2 cut(s) 289, 382
CfoI GCGC 2 cut(s) 2404, 2473
Cfr10I RCCGGY 2 cut(s) 2122, 2221
Cfr13I GGNCC 3 cut(s) 845, 1292, 2458
Csp6I GTAC 8 cut(s) 255, 270, 632, 1217, 1305, 1323, 1691, 2032
CviQI GTAC 8 cut(s) 255, 270, 632, 1217, 1305, 1323, 1691, 2032
DdeI CTNAG 6 cut(s) 123, 165, 956, 1161, 1962, 2217
DpnI GATC 8 cut(s) 23, 340, 795, 1980, 2012, 2106, 2414, 2424
DpnII GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
DraI TTTAAA 1 cut(s) 576
DraIII CACNNNGTG 1 cut(s) 1438
EaeI YGGCCR 1 cut(s) 1718
Eam1104I CTCTTC 2 cut(s) 733, 2333
EarI CTCTTC 2 cut(s) 733, 2333
Eco130I CCWWGG 1 cut(s) 871
Eco32I GATATC 4 cut(s) 178, 1534, 1663, 2229
Eco47I GGWCC 2 cut(s) 845, 2458
Eco57I CTGAAG 2 cut(s) 120, 1011
Eco72I CACGTG 1 cut(s) 1310
EcoO109I RGGNCCY 2 cut(s) 1292, 2458
EcoRII CCWGG 4 cut(s) 400, 610, 753, 1536
EcoRV GATATC 4 cut(s) 178, 1534, 1663, 2229
EcoT14I CCWWGG 1 cut(s) 871
EcoT22I ATGCAT 2 cut(s) 1125, 1600
ErhI CCWWGG 1 cut(s) 871
FalI AAGNNNNNCTT 4 cut(s) 1918, 1950, 2285, 2317
FaqI GGGAC 4 cut(s) 872, 984, 1300, 2471
FauI CCCGC 1 cut(s) 592
FauNDI CATATG 1 cut(s) 1226
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 9 cut(s) 14, 1261, 1355, 1466, 1494, 1842, 2120, 2298, 2369
FokI GGATG 1 cut(s) 1775
Fsp4HI GCNGC 9 cut(s) 14, 1261, 1355, 1466, 1494, 1842, 2120, 2298, 2369
FspBI CTAG 3 cut(s) 692, 771, 872
GlaI GCGC 2 cut(s) 2403, 2472
GluI GCNGC 9 cut(s) 14, 1261, 1355, 1466, 1494, 1842, 2120, 2298, 2369
GsaI CCCAGC 1 cut(s) 2048
GsuI CTGGAG 3 cut(s) 501, 1194, 1467
HaeIII GGCC 3 cut(s) 1294, 1720, 2261
HapII CCGG 5 cut(s) 1572, 2123, 2222, 2262, 2497
HhaI GCGC 2 cut(s) 2404, 2473
Hin6I GCGC 2 cut(s) 2402, 2471
HinP1I GCGC 2 cut(s) 2402, 2471
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 8 cut(s) 87, 127, 529, 1568, 1814, 1892, 2331, 2480
HpaII CCGG 5 cut(s) 1572, 2123, 2222, 2262, 2497
HphI GGTGA 8 cut(s) 161, 416, 887, 1552, 1766, 2101, 2236, 2407
Hpy166II GTNNAC 8 cut(s) 237, 475, 1307, 1323, 1362, 1543, 1649, 2034
Hpy188I TCNGA 5 cut(s) 126, 465, 1891, 1965, 2135
Hpy8I GTNNAC 8 cut(s) 237, 475, 1307, 1323, 1362, 1543, 1649, 2034
HpyCH4III ACNGT 9 cut(s) 241, 286, 715, 916, 1012, 1419, 1631, 1738, 2419
HpyCH4IV ACGT 6 cut(s) 67, 553, 1309, 1319, 2361, 2436
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 593, 1129
HpyF3I CTNAG 6 cut(s) 123, 165, 956, 1161, 1962, 2217
HpySE526I ACGT 6 cut(s) 67, 553, 1309, 1319, 2361, 2436
HspAI GCGC 2 cut(s) 2402, 2471
KroI GCCGGC 1 cut(s) 2122
KroNI GCCGGC 1 cut(s) 2124
Kzo9I GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
LmnI GCTCC 6 cut(s) 1199, 1448, 1868, 1970, 2135, 2246
Lsp1109I GCAGC 7 cut(s) 1341, 1452, 1480, 1828, 2131, 2284, 2380
LweI GCATC 3 cut(s) 757, 2182, 2389
MaeI CTAG 3 cut(s) 692, 771, 872
MaeII ACGT 6 cut(s) 67, 553, 1309, 1319, 2361, 2436
MaeIII GTNAC 5 cut(s) 280, 506, 1189, 1430, 2440
MalI GATC 8 cut(s) 23, 340, 795, 1980, 2012, 2106, 2414, 2424
MboI GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
MboII GAAGA 9 cut(s) 203, 750, 770, 803, 1535, 1808, 2104, 2122, 2350
MfeI CAATTG 3 cut(s) 706, 1235, 1504
MflI RGATCY 3 cut(s) 338, 793, 2422
MhlI GDGCHC 1 cut(s) 1587
MlyI GAGTC 3 cut(s) 81, 1886, 2325
MmeI TCCRAC 3 cut(s) 481, 1914, 2382
Mph1103I ATGCAT 2 cut(s) 1125, 1600
MroNI GCCGGC 1 cut(s) 2122
MroXI GAANNNNTTC 1 cut(s) 2504
MseI TTAA 8 cut(s) 575, 719, 747, 1071, 1349, 1562, 2072, 2515
MslI CAYNNNNRTG 2 cut(s) 387, 1760
MspA1I CMGCKG 3 cut(s) 587, 596, 1946
MspI CCGG 5 cut(s) 1572, 2123, 2222, 2262, 2497
MspR9I CCNGG 5 cut(s) 402, 612, 755, 1538, 2497
MssI GTTTAAAC 1 cut(s) 576
MunI CAATTG 3 cut(s) 706, 1235, 1504
MvaI CCWGG 4 cut(s) 402, 612, 755, 1538
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 3 cut(s) 116, 593, 1129
NaeI GCCGGC 1 cut(s) 2124
NciI CCSGG 1 cut(s) 2497
NdeI CATATG 1 cut(s) 1226
NdeII GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
NgoMIV GCCGGC 1 cut(s) 2122
NlaIV GGNNCC 2 cut(s) 1972, 2424
NmuCI GTSAC 4 cut(s) 280, 506, 1430, 2440
NsiI ATGCAT 2 cut(s) 1125, 1600
NspI RCATGY 3 cut(s) 1655, 1759, 2469
PagI TCATGA 2 cut(s) 289, 382
PciI ACATGT 1 cut(s) 2465
PcsI WCGNNNNNNNCGW 1 cut(s) 73
PdiI GCCGGC 1 cut(s) 2124
PdmI GAANNNNTTC 1 cut(s) 2504
PfeI GAWTC 5 cut(s) 127, 529, 1568, 1814, 2480
PfoI TCCNGGA 1 cut(s) 2495
PkrI GCNGC 9 cut(s) 15, 1262, 1356, 1467, 1495, 1843, 2121, 2299, 2370
PleI GAGTC 3 cut(s) 81, 1886, 2325
PmaCI CACGTG 1 cut(s) 1310
PmeI GTTTAAAC 1 cut(s) 576
PmlI CACGTG 1 cut(s) 1310
PpsI GAGTC 3 cut(s) 81, 1886, 2325
Ppu21I YACGTR 2 cut(s) 1310, 1320
PpuMI RGGWCCY 1 cut(s) 2458
PscI ACATGT 1 cut(s) 2465
PsiI TTATAA 1 cut(s) 437
Psp5II RGGWCCY 1 cut(s) 2458
Psp6I CCWGG 4 cut(s) 400, 610, 753, 1536
PspCI CACGTG 1 cut(s) 1310
PspFI CCCAGC 1 cut(s) 2044
PspGI CCWGG 4 cut(s) 400, 610, 753, 1536
PspN4I GGNNCC 2 cut(s) 1972, 2424
PspPI GGNCC 3 cut(s) 845, 1292, 2458
PspPPI RGGWCCY 1 cut(s) 2458
PsrI GAACNNNNNNTAC 2 cut(s) 1603, 1635
PstI CTGCAG 3 cut(s) 1198, 1359, 1846
PstNI CAGNNNCTG 1 cut(s) 1295
PsuI RGATCY 3 cut(s) 338, 793, 2422
PvuII CAGCTG 2 cut(s) 596, 1946
RsaI GTAC 8 cut(s) 256, 271, 633, 1218, 1306, 1324, 1692, 2033
RsaNI GTAC 8 cut(s) 255, 270, 632, 1217, 1305, 1323, 1691, 2032
RseI CAYNNNNRTG 2 cut(s) 387, 1760
SaqAI TTAA 8 cut(s) 575, 719, 747, 1071, 1349, 1562, 2072, 2515
SatI GCNGC 9 cut(s) 14, 1261, 1355, 1466, 1494, 1842, 2120, 2298, 2369
Sau3AI GATC 8 cut(s) 21, 338, 793, 1978, 2010, 2104, 2412, 2422
Sau96I GGNCC 3 cut(s) 845, 1292, 2458
SchI GAGTC 3 cut(s) 81, 1886, 2325
ScrFI CCNGG 5 cut(s) 402, 612, 755, 1538, 2497
SduI GDGCHC 1 cut(s) 1587
SfaNI GCATC 3 cut(s) 757, 2182, 2389
SfcI CTRYAG 6 cut(s) 105, 1194, 1355, 1737, 1842, 2049
SinI GGWCC 2 cut(s) 845, 2458
SmiMI CAYNNNNRTG 2 cut(s) 387, 1760
SmlI CTYRAG 4 cut(s) 131, 2005, 2302, 2333
SmoI CTYRAG 4 cut(s) 131, 2005, 2302, 2333
SsiI CCGC 4 cut(s) 14, 585, 1260, 2351
SspI AATATT 1 cut(s) 643
SspMI CTAG 3 cut(s) 692, 771, 872
StyD4I CCNGG 5 cut(s) 400, 610, 753, 1536, 2495
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 9 cut(s) 241, 286, 715, 916, 1012, 1419, 1631, 1738, 2419
TaiI ACGT 6 cut(s) 70, 556, 1312, 1322, 2364, 2439
TaqI TCGA 5 cut(s) 24, 249, 799, 1338, 2382
TatI WGTACW 4 cut(s) 631, 1216, 1690, 2031
TauI GCSGC 2 cut(s) 16, 1263
TfiI GAWTC 5 cut(s) 127, 529, 1568, 1814, 2480
Tru1I TTAA 8 cut(s) 575, 719, 747, 1071, 1349, 1562, 2072, 2515
Tru9I TTAA 8 cut(s) 575, 719, 747, 1071, 1349, 1562, 2072, 2515
TscAI CASTG 6 cut(s) 103, 484, 498, 859, 1257, 1636
TseFI GTSAC 4 cut(s) 280, 506, 1430, 2440
TseI GCWGC 7 cut(s) 1354, 1465, 1493, 1841, 2119, 2297, 2368
Tsp45I GTSAC 4 cut(s) 280, 506, 1430, 2440
TspGWI ACGGA 1 cut(s) 1323
TspRI CASTG 6 cut(s) 103, 484, 498, 859, 1257, 1636
VpaK11BI GGWCC 2 cut(s) 845, 2458
XapI RAATTY 1 cut(s) 368
XceI RCATGY 3 cut(s) 1655, 1759, 2469
XmaJI CCTAGG 1 cut(s) 871
XmiI GTMKAC 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 2504
XspI CTAG 3 cut(s) 692, 771, 872
Zsp2I ATGCAT 2 cut(s) 1125, 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.