Rroxscaffold_5G00372620
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002839
Physical Location & Seq
Forward (+)
54018683 .. 54025404
6722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372620.1

Sequence Viewer

Length: 2532 bp
ATGAAGCTGGTTCCAAGAGAGCTTGACAAATTGGACCTCCACAATGCTGGGTTTCTAGCCCAGAAGAGACTTGCTCGTGGGTTAAGGCTCAATCACCCTGAAGCTGTAGCACTCATAGCCACTCAGATATTGGAGTTTGTTAGAGATGGTGACAAATCTGTGGCAGAGTTGATGGATATTGGGAAACAGCTTCTAGGGAGGAAGCAAGTTCTTCCGGGTGTTCAATATCTATTGGATATGGTGCAGGTTGAGGGGACGTTTCGTGATGGGACCAAGTTAATCACCATTCATGATCCAATTTCTTGTGATAATGGAGATCTGGAACTAGCTTTAAAGAACTCTTTTCTTCCTGTTATGAAACGTACTTTTTCTGCAGTTCCCTCAATTGATAAGTTTCCGGAAATGGTGGATGGTGGAGTTCCTGGTGAAATAATCTTCGAAGGTGGTAATATTGTAATTAATGAAGGAAGGAAGGCAATAATTCTGAAAATTGTCAATACTGGAGACAGGCCAGTTCAGGTTGGCAGTCACTATCACTTTATTGAGGTCAATCCCCACTTGGTCTTTGATCGAATGAAATCATATGGTATGCGCCTGAATATACCTGCTGGAACAGCCAAGAGATTTGAGCCGGGGGAATGTAAACATGTCGTACTTGTAAGCATCGGAGGTAATAGAGTAATCAGAGGAGGAAATGACATTGTTGATGGTGCAGTACGAAGCTTTGACAGATACCAAGTAGTCAAGGGAGCTTTGGAAGCCAGACGATTTGGGAATGAAGAAGAACCAAATGCTAGGGAAGGTGTTGCAGGAATAGATGCAGCTTTTACCACTGTAATTTCTCGTGAGTCATATGCAAACATGTATGGCCCTACTACCGGTGATAAAGTTCGGCTCGGTGATACCAACTTGTTTGCTGAAATTGAAAAAGATTATACTGTTTATGGGGATGAATCTGTATTTGGAGGTGGCAAAGTTATAAGAGACGGAATGGGCCAGTCATCAGGGAATGAACCAGCTGACTCATTGGACACAGTTATAACTAATGCTGTAATCATTGATTATAGTGGGATCTTCAAGGCAGATATTGGTATTAAAGATGGTCATATAGTTGCCCTTGGGAAATCAGGCAATCCAGATGTTATGGATGGTGTATCTTGCAATATGATCATTGGGGTTAACACGGAGGTCATTGCAGCGGAAGGACAAATTGTGACTGCAGGAGCTATAGACTGTCATGTGCATTTCATATGCCCTCAGTTGGCATATGAAGCGATATCAAGTGGTATCACAACCCTAGTGGGAGGTGGAACAGGACCTGCTACGGGAACTCGTGCAACAACATGCACACCATCTCCATTGCAAATGAAGTTAATGCTGCAATCAACTGATGAAGTGCCTCTAAATTTTGGCTTTACAGGAAAGGGGAACAGTGCCAAACCTGATGGCCTACATGAGATAATCAAGGCTGGAGCAATGGGTCTGAAGCTGCATGAAGACTGGGGCACAACTCCTGCTGCAATAGACAATTGTTTGAGTGTTGGAGACCAATATGACGTCCAGGTTAATATCCACACGGACACCTTGAATGAATCTGGATTTGTGGAACACACAATCGCTGCATTTAAAGACAGAACTATCCATACTTACCACAGTGAAGGTGCAGGTGGGGGTCATGCTCCAGATATTATCAAAGTATGTGGTGTGGAAAATGTCCTGCCCTCATCAACGAATCCCACACGGCCTTTCACCTCCAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTAGCTTTTGCTGAATCAAGGATAAGAGCTGAAACAATTGCCGCAGAAGATATATTGCATGATATGGGGGCGATTAGCATGGTAGCTTCTGATTCACAGGCTATGGGTCGCATTGGAGAGGTGATAAGCAGAACTTGGCAAACTGCTGACAAGATGAAATCACAAAGAGGGTCAATAGAACCTAGTGGATCCAACAATGACAATCTCCGGATCAAGAGATACATTGCAAAGTACACCATAAATCCAGCAATAGCAAATGGATTTTCTACATATGTTGGTTCCGTTCAGGCGGGCAAGTTCGCTGATCTTGTTCTATGGAAGCCATCTTTCTTTGGGGCTAAACCAGAAATGGTGATCAAAGGTGGTGCAATTGCATGGGCTAATATGGGTGATCCAAACGCAAGCATCCCGACACCTGAACCGGTCATTTCAAGACCTATGTTTGGTGCATTTGGCAAGGCTAGTAGTGCTCACTCCATTGCCTTTGAAGCTAAAAAAAATGGGGTTAAAGCCTTGTATGACCTGAACAAGAGAGTGGAAGCTGTGGGCAATGTTAGGAATCTCCGCAAACGCGACATGGAGCTCAATGACGCCCTGCCAAATATTAAAGTTGACCCAGAGACATACACGGTGACAGCAGACGGCGAGGATCTGACCTGCAGTGCAGCCACCACCGTTCCCCTATCCCGGAATTACTTCCTGTTTTAG

Protein Analysis

843

Amino Acids

90.87

Weight (kDa)

5.56

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urease_gamma PF00547 1 - 100 1e-41 Urease, gamma subunit
Urease_beta PF00699 141 - 237 4.8e-41 Urease beta subunit
Urease_alpha PF00449 280 - 398 9.7e-57 Urease alpha-subunit, N-terminal domain
Amidohydro_1 PF01979 404 - 730 1e-75 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 980, 1040
AarI CACCTGC 1 cut(s) 1655
AatII GACGTC 1 cut(s) 1560
Acc36I ACCTGC 5 cut(s) 235, 613, 1327, 1655, 2489
AccII CGCG 1 cut(s) 2397
AccIII TCCGGA 2 cut(s) 397, 2031
AciI CCGC 4 cut(s) 1199, 1866, 2114, 2389
AclWI GGATC 7 cut(s) 287, 1079, 2007, 2020, 2042, 2210, 2481
AcsI RAATTY 1 cut(s) 1405
AcuI CTGAAG 2 cut(s) 120, 1505
AcyI GRCGYC 2 cut(s) 1557, 2415
AfaI GTAC 4 cut(s) 364, 654, 717, 2057
AfiI CCNNNNNNNGG 6 cut(s) 878, 1261, 1325, 1807, 2267, 2511
AflIII ACRYGT 2 cut(s) 646, 861
AgeI ACCGGT 2 cut(s) 878, 2245
AgsI TTSAA 6 cut(s) 224, 926, 1078, 1588, 2256, 2312
AjnI CCWGG 2 cut(s) 421, 1560
AjuI GAANNNNNNNTTGG 2 cut(s) 945, 977
Alw21I GWGCWC 2 cut(s) 2296, 2409
Alw26I GTCTC 5 cut(s) 61, 498, 978, 1539, 2438
AlwI GGATC 7 cut(s) 287, 1079, 2007, 2020, 2042, 2210, 2481
AlwNI CAGNNNCTG 1 cut(s) 1319
Aor13HI TCCGGA 2 cut(s) 397, 2031
AoxI GGCC 5 cut(s) 509, 868, 994, 1447, 1742
ApeKI GCWGC 7 cut(s) 821, 1196, 1378, 1489, 1517, 1619, 2489
ApoI RAATTY 1 cut(s) 1405
AseI ATTAAT 1 cut(s) 459
AsiGI ACCGGT 2 cut(s) 878, 2245
Asp700I GAANNNNTTC 1 cut(s) 2519
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 5 cut(s) 34, 270, 869, 994, 1316
AsuC2I CCSGG 3 cut(s) 216, 633, 2512
AsuII TTCGAA 1 cut(s) 438
AvaII GGWCC 3 cut(s) 34, 270, 1316
BaeGI GKGCMC 1 cut(s) 1508
BamHI GGATCC 1 cut(s) 2012
BanII GRGCYC 1 cut(s) 2409
BauI CACGAG 3 cut(s) 75, 843, 1332
BbsI GAAGAC 1 cut(s) 1503
Bbv12I GWGCWC 2 cut(s) 2296, 2409
BbvI GCAGC 7 cut(s) 833, 1208, 1365, 1476, 1504, 1606, 2501
BceAI ACGGC 2 cut(s) 1757, 2482
BciT130I CCWGG 2 cut(s) 423, 1562
BclI TGATCA 2 cut(s) 1167, 2178
BcnI CCSGG 3 cut(s) 216, 633, 2512
BcoDI GTCTC 5 cut(s) 61, 498, 978, 1539, 2438
BfaI CTAG 7 cut(s) 56, 194, 326, 795, 1298, 2007, 2286
BfmI CTRYAG 6 cut(s) 105, 372, 1218, 1227, 1761, 2482
BfuAI ACCTGC 5 cut(s) 235, 613, 1327, 1655, 2489
BglII AGATCT 1 cut(s) 316
BisI GCNGC 8 cut(s) 822, 1197, 1379, 1490, 1518, 1620, 1866, 2490
BlsI GCNGC 8 cut(s) 823, 1198, 1380, 1491, 1519, 1621, 1867, 2491
Bme1390I CCNGG 5 cut(s) 216, 423, 633, 1562, 2512
Bme18I GGWCC 3 cut(s) 34, 270, 1316
BmgT120I GGNCC 5 cut(s) 34, 270, 869, 994, 1316
BmiI GGNNCC 4 cut(s) 12, 271, 2014, 2104
BmrFI CCNGG 5 cut(s) 216, 423, 633, 1562, 2512
BmrI ACTGGG 1 cut(s) 1510
BmsI GCATC 4 cut(s) 672, 808, 1780, 2238
BmuI ACTGGG 1 cut(s) 1510
BpiI GAAGAC 1 cut(s) 1503
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 3 cut(s) 522, 1491, 1665
Bpu14I TTCGAA 1 cut(s) 438
BpuMI CCSGG 3 cut(s) 216, 633, 2512
BsaBI GATNNNNATC 1 cut(s) 1770
BsaHI GRCGYC 2 cut(s) 1557, 2415
BsaI GGTCTC 1 cut(s) 1539
BsaJI CCNNGG 2 cut(s) 632, 1117
BsaWI WCCGGW 4 cut(s) 397, 878, 2031, 2245
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bsc4I CCNNNNNNNGG 6 cut(s) 878, 1261, 1325, 1807, 2267, 2511
Bse118I RCCGGY 2 cut(s) 878, 2245
Bse1I ACTGG 4 cut(s) 505, 512, 997, 1505
Bse3DI GCAATG 6 cut(s) 1191, 1358, 1482, 2046, 2301, 2380
Bse8I GATNNNNATC 1 cut(s) 1770
BseAI TCCGGA 2 cut(s) 397, 2031
BseBI CCWGG 2 cut(s) 423, 1562
BseDI CCNNGG 2 cut(s) 632, 1117
BseGI GGATG 4 cut(s) 415, 955, 1153, 2229
BseJI GATNNNNATC 1 cut(s) 1770
BseLI CCNNNNNNNGG 6 cut(s) 878, 1261, 1325, 1807, 2267, 2511
BseMI GCAATG 6 cut(s) 1191, 1358, 1482, 2046, 2301, 2380
BseMII CTCAG 2 cut(s) 137, 1271
BseNI ACTGG 4 cut(s) 505, 512, 997, 1505
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 1 cut(s) 1508
BseXI GCAGC 7 cut(s) 833, 1208, 1365, 1476, 1504, 1606, 2501
BseYI CCCAGC 1 cut(s) 47
BsgI GTGCAG 4 cut(s) 263, 732, 1683, 2508
Bsh1236I CGCG 1 cut(s) 2397
BshFI GGCC 5 cut(s) 511, 870, 996, 1449, 1744
BshTI ACCGGT 2 cut(s) 878, 2245
BsiHKAI GWGCWC 2 cut(s) 2296, 2409
BsiSI CCGG 7 cut(s) 215, 398, 632, 879, 2032, 2246, 2512
BslFI GGGAC 2 cut(s) 268, 283
BslI CCNNNNNNNGG 6 cut(s) 878, 1261, 1325, 1807, 2267, 2511
BsmAI GTCTC 5 cut(s) 61, 498, 978, 1539, 2438
BsmBI CGTCTC 1 cut(s) 978
BsmFI GGGAC 2 cut(s) 268, 283
BsnI GGCC 5 cut(s) 511, 870, 996, 1449, 1744
Bso31I GGTCTC 1 cut(s) 1539
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 3 cut(s) 1508, 2296, 2409
Bsp13I TCCGGA 2 cut(s) 397, 2031
BspACI CCGC 4 cut(s) 1199, 1866, 2114, 2389
BspANI GGCC 5 cut(s) 511, 870, 996, 1449, 1744
BspCNI CTCAG 2 cut(s) 136, 1270
BspEI TCCGGA 2 cut(s) 397, 2031
BspFNI CGCG 1 cut(s) 2397
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 4 cut(s) 12, 271, 2014, 2104
BspMAI CTGCAG 3 cut(s) 376, 1222, 2486
BspMI ACCTGC 5 cut(s) 235, 613, 1327, 1655, 2489
BspPI GGATC 7 cut(s) 287, 1079, 2007, 2020, 2042, 2210, 2481
BspT104I TTCGAA 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 1539
BsrDI GCAATG 6 cut(s) 1191, 1358, 1482, 2046, 2301, 2380
BsrFI RCCGGY 2 cut(s) 878, 2245
BsrI ACTGG 4 cut(s) 505, 512, 997, 1505
BssAI RCCGGY 2 cut(s) 878, 2245
BssECI CCNNGG 2 cut(s) 632, 1117
BssNI GRCGYC 2 cut(s) 1557, 2415
BssSI CACGAG 3 cut(s) 75, 843, 1332
BssT1I CCWWGG 1 cut(s) 1117
Bst2BI CACGAG 3 cut(s) 75, 843, 1332
Bst2UI CCWGG 2 cut(s) 423, 1562
Bst4CI ACNGT 8 cut(s) 835, 940, 1036, 1235, 1433, 1655, 2455, 2500
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 2 cut(s) 1557, 2415
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 2116, 2227
BstDEI CTNAG 2 cut(s) 123, 1257
BstENI CCTNNNNNAGG 1 cut(s) 1805
BstF5I GGATG 4 cut(s) 415, 955, 1153, 2229
BstFNI CGCG 1 cut(s) 2397
BstHHI GCGC 1 cut(s) 594
BstMAI GTCTC 5 cut(s) 61, 498, 978, 1539, 2438
BstMWI GCNNNNNNNGC 6 cut(s) 116, 614, 758, 1271, 2291, 2312
BstNI CCWGG 2 cut(s) 423, 1562
BstNSI RCATGY 3 cut(s) 650, 865, 1347
BstSCI CCNGG 5 cut(s) 214, 421, 631, 1560, 2510
BstSFI CTRYAG 6 cut(s) 105, 372, 1218, 1227, 1761, 2482
BstSLI GKGCMC 1 cut(s) 1508
BstUI CGCG 1 cut(s) 2397
BstV1I GCAGC 7 cut(s) 833, 1208, 1365, 1476, 1504, 1606, 2501
BstV2I GAAGAC 1 cut(s) 1503
BstX2I RGATCY 4 cut(s) 316, 1071, 2012, 2473
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 4 cut(s) 316, 1071, 2012, 2473
BsuRI GGCC 5 cut(s) 511, 870, 996, 1449, 1744
BtsCI GGATG 4 cut(s) 415, 955, 1153, 2229
BtsI GCAGTG 1 cut(s) 2491
BtsIMutI CAGTG 4 cut(s) 831, 1438, 1660, 2491
BveI ACCTGC 5 cut(s) 235, 613, 1327, 1655, 2489
Cac8I GCNNGC 2 cut(s) 2116, 2227
CaiI CAGNNNCTG 1 cut(s) 1319
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 594
Cfr10I RCCGGY 2 cut(s) 878, 2245
Cfr13I GGNCC 5 cut(s) 34, 270, 869, 994, 1316
CseI GACGC 1 cut(s) 2423
Csp6I GTAC 4 cut(s) 363, 653, 716, 2056
CspAI ACCGGT 2 cut(s) 878, 2245
CspCI CAANNNNNGTGG 4 cut(s) 1281, 1316, 1681, 1716
CviQI GTAC 4 cut(s) 363, 653, 716, 2056
DdeI CTNAG 2 cut(s) 123, 1257
DraI TTTAAA 2 cut(s) 333, 1627
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 2407
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 1 cut(s) 2409
Eco31I GGTCTC 1 cut(s) 1539
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 3 cut(s) 34, 270, 1316
Eco53kI GAGCTC 1 cut(s) 2407
Eco57I CTGAAG 2 cut(s) 120, 1505
EcoICRI GAGCTC 1 cut(s) 2407
EcoNI CCTNNNNNAGG 1 cut(s) 1805
EcoO109I RGGNCCY 1 cut(s) 1316
EcoRII CCWGG 2 cut(s) 421, 1560
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 1 cut(s) 1117
EcoT38I GRGCYC 1 cut(s) 2409
ErhI CCWWGG 1 cut(s) 1117
Esp3I CGTCTC 1 cut(s) 978
FalI AAGNNNNNCTT 2 cut(s) 1942, 1974
FaqI GGGAC 2 cut(s) 268, 283
FauI CCCGC 1 cut(s) 2107
FauNDI CATATG 5 cut(s) 583, 853, 1250, 1267, 2095
FbaI TGATCA 2 cut(s) 1167, 2178
Fnu4HI GCNGC 8 cut(s) 822, 1197, 1379, 1490, 1518, 1620, 1866, 2490
FokI GGATG 4 cut(s) 422, 962, 1160, 2216
FriOI GRGCYC 1 cut(s) 2409
Fsp4HI GCNGC 8 cut(s) 822, 1197, 1379, 1490, 1518, 1620, 1866, 2490
FspBI CTAG 7 cut(s) 56, 194, 326, 795, 1298, 2007, 2286
GlaI GCGC 1 cut(s) 593
GluI GCNGC 8 cut(s) 822, 1197, 1379, 1490, 1518, 1620, 1866, 2490
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 3 cut(s) 522, 1491, 1665
HaeIII GGCC 5 cut(s) 511, 870, 996, 1449, 1744
HapII CCGG 7 cut(s) 215, 398, 632, 879, 2032, 2246, 2512
HgaI GACGC 1 cut(s) 2423
HhaI GCGC 1 cut(s) 594
Hin1I GRCGYC 2 cut(s) 1557, 2415
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HincII GTYRAC 2 cut(s) 1180, 2437
HindII GTYRAC 2 cut(s) 1180, 2437
HindIII AAGCTT 1 cut(s) 721
HinfI GANTC 8 cut(s) 848, 953, 1022, 1592, 1732, 1838, 1916, 2383
HpaI GTTAAC 1 cut(s) 1180
HpaII CCGG 7 cut(s) 215, 398, 632, 879, 2032, 2246, 2512
Hpy166II GTNNAC 4 cut(s) 644, 1180, 2058, 2437
Hpy188I TCNGA 7 cut(s) 126, 486, 668, 686, 1485, 1915, 2478
Hpy8I GTNNAC 4 cut(s) 644, 1180, 2058, 2437
HpyAV CCTTC 7 cut(s) 434, 458, 462, 466, 794, 1196, 1652
HpyCH4III ACNGT 8 cut(s) 835, 940, 1036, 1235, 1433, 1655, 2455, 2500
HpyCH4IV ACGT 3 cut(s) 257, 361, 1557
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 614, 758, 1271, 2291, 2312
HpyF3I CTNAG 2 cut(s) 123, 1257
HpySE526I ACGT 3 cut(s) 257, 361, 1557
Hsp92I GRCGYC 2 cut(s) 1557, 2415
HspAI GCGC 1 cut(s) 592
Kpn2I TCCGGA 2 cut(s) 397, 2031
Ksp22I TGATCA 2 cut(s) 1167, 2178
KspAI GTTAAC 1 cut(s) 1180
LmnI GCTCC 5 cut(s) 749, 1223, 1472, 1684, 2404
Lsp1109I GCAGC 7 cut(s) 833, 1208, 1365, 1476, 1504, 1606, 2501
LweI GCATC 4 cut(s) 672, 808, 1780, 2238
MaeI CTAG 7 cut(s) 56, 194, 326, 795, 1298, 2007, 2286
MaeII ACGT 3 cut(s) 257, 361, 1557
MaeIII GTNAC 4 cut(s) 149, 527, 1213, 2455
MfeI CAATTG 4 cut(s) 384, 1528, 1860, 2193
MflI RGATCY 4 cut(s) 316, 1071, 2012, 2473
MhlI GDGCHC 3 cut(s) 1508, 2296, 2409
MlyI GAGTC 2 cut(s) 857, 1016
MmeI TCCRAC 2 cut(s) 1522, 2040
MroI TCCGGA 2 cut(s) 397, 2031
MroXI GAANNNNTTC 1 cut(s) 2519
MspA1I CMGCKG 2 cut(s) 1019, 1199
MspI CCGG 7 cut(s) 215, 398, 632, 879, 2032, 2246, 2512
MspR9I CCNGG 5 cut(s) 216, 423, 633, 1562, 2512
MunI CAATTG 4 cut(s) 384, 1528, 1860, 2193
MvaI CCWGG 2 cut(s) 423, 1562
MvnI CGCG 1 cut(s) 2397
MwoI GCNNNNNNNGC 6 cut(s) 116, 614, 758, 1271, 2291, 2312
NciI CCSGG 3 cut(s) 216, 633, 2512
NdeI CATATG 5 cut(s) 583, 853, 1250, 1267, 2095
NlaIV GGNNCC 4 cut(s) 12, 271, 2014, 2104
NmuCI GTSAC 4 cut(s) 149, 527, 1213, 2455
NspI RCATGY 3 cut(s) 650, 865, 1347
NspV TTCGAA 1 cut(s) 438
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 1655
PciI ACATGT 2 cut(s) 646, 861
PdmI GAANNNNTTC 1 cut(s) 2519
PfeI GAWTC 6 cut(s) 953, 1592, 1732, 1838, 1916, 2383
PfoI TCCNGGA 1 cut(s) 2510
PinAI ACCGGT 2 cut(s) 878, 2245
PkrI GCNGC 8 cut(s) 823, 1198, 1380, 1491, 1519, 1621, 1867, 2491
PleI GAGTC 2 cut(s) 856, 1016
PpsI GAGTC 2 cut(s) 856, 1016
PpuMI RGGWCCY 1 cut(s) 1316
PscI ACATGT 2 cut(s) 646, 861
PshBI ATTAAT 1 cut(s) 459
PsiI TTATAA 2 cut(s) 980, 1040
Psp124BI GAGCTC 1 cut(s) 2409
Psp5II RGGWCCY 1 cut(s) 1316
Psp6I CCWGG 2 cut(s) 421, 1560
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 421, 1560
PspN4I GGNNCC 4 cut(s) 12, 271, 2014, 2104
PspPI GGNCC 5 cut(s) 34, 270, 869, 994, 1316
PspPPI RGGWCCY 1 cut(s) 1316
PsrI GAACNNNNNNTAC 2 cut(s) 1627, 1659
PstI CTGCAG 3 cut(s) 376, 1222, 2486
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 4 cut(s) 316, 1071, 2012, 2473
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 4 cut(s) 364, 654, 717, 2057
RsaNI GTAC 4 cut(s) 363, 653, 716, 2056
SacI GAGCTC 1 cut(s) 2409
SatI GCNGC 8 cut(s) 822, 1197, 1379, 1490, 1518, 1620, 1866, 2490
Sau96I GGNCC 5 cut(s) 34, 270, 869, 994, 1316
SchI GAGTC 2 cut(s) 857, 1016
ScrFI CCNGG 5 cut(s) 216, 423, 633, 1562, 2512
SduI GDGCHC 3 cut(s) 1508, 2296, 2409
SfaNI GCATC 4 cut(s) 672, 808, 1780, 2238
SfcI CTRYAG 6 cut(s) 105, 372, 1218, 1227, 1761, 2482
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 3 cut(s) 34, 270, 1316
SsiI CCGC 4 cut(s) 1199, 1866, 2114, 2389
SspI AATATT 2 cut(s) 451, 2428
SspMI CTAG 7 cut(s) 56, 194, 326, 795, 1298, 2007, 2286
SstI GAGCTC 1 cut(s) 2409
StyD4I CCNGG 5 cut(s) 214, 421, 631, 1560, 2510
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 8 cut(s) 835, 940, 1036, 1235, 1433, 1655, 2455, 2500
TaiI ACGT 3 cut(s) 260, 364, 1560
TaqI TCGA 2 cut(s) 438, 571
TatI WGTACW 1 cut(s) 2055
TauI GCSGC 1 cut(s) 1868
TfiI GAWTC 6 cut(s) 953, 1592, 1732, 1838, 1916, 2383
TscAI CASTG 4 cut(s) 838, 1438, 1660, 2491
TseFI GTSAC 4 cut(s) 149, 527, 1213, 2455
TseI GCWGC 7 cut(s) 821, 1196, 1378, 1489, 1517, 1619, 2489
Tsp45I GTSAC 4 cut(s) 149, 527, 1213, 2455
TspGWI ACGGA 4 cut(s) 1002, 1199, 1592, 2095
TspRI CASTG 4 cut(s) 838, 1438, 1660, 2491
VpaK11BI GGWCC 3 cut(s) 34, 270, 1316
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 1805
XapI RAATTY 1 cut(s) 1405
XceI RCATGY 3 cut(s) 650, 865, 1347
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 2519
XspI CTAG 7 cut(s) 56, 194, 326, 795, 1298, 2007, 2286
ZraI GACGTC 1 cut(s) 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.