RchiOBHm_Chr4g0430931
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002839
Physical Location & Seq
Forward (+)
55290468 .. 55297177
6710 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39964

Sequence Viewer

Length: 2493 bp
ATGAAGCTGGTTCCAAGAGAGCTTGACAAATTGGGCCTCCACAATGCTGGGTTTCTAGCCCAGAAGAGACTTGCTCGTGGGTTAAGGCTCAATCACCCTGAAGCTGTTGCACTCATAGCCACTCAGATATTGGAGTTTGTTAGAGATGGTGACAAATCTGTGGCAGAGTTGATGGATATTGGGAAACAGCTTCTAGGGAGGAAGCAAGTTCTTCCGGGTGTTCAATATCTATTGGATATGGTGCAGGTCGAGGGGACGTTTCGTGATGGGACCAAGTTAATCACCATTCATGATCCAATTTCTTGTGATAATGGAGATCTGGAACTAGCTTTAAAGAACTCTTTTCTTCCTGTTCCCTCAATTGATAAGTTTCCAGAAATGGTGGACGGTAGAGTTCCTGGTGAAATAATCGTCAAAGGTGGTAATATTGTAATTAATGAAGGAAGGAAGGCAATAATTCTGAAAGTTGTCAATACTGGAGACAGGCCAGTTCAGGTTGGCAGTCACTATCACTTTATTGAGGTCAATCCCCACCTGGTCTTTGATCGAATGAAATCATATGGTATGCGCCTGAATATACCTGCTGGAACAGCCAAGAGATTTGAGCCGGGGGAATGTAAACATGTCGTACTTGTAAGCATTGGAGTCAGAGGAGGAAATGACATTGTTGATGGTGCAGTACGAAGCCAAGCAGTCAAGGGAGCTTTGGAAGCCAGACAATTTGGGAATGAAGAAGAACCAAATGCTAGGGAAGGTGTTGCAGGAATAGATGCAGCTTTTACCACTGTAATTTCTCGTGAGTCATATGCAAACATGTATGGCCCTACTACTGGTGATAAAGTTCGGCTCGGTGATACCAACTTGTTTGCTGAAATTGAAAAAGATTATACTTTTTGTGGGGATGAATCTGTATTTGGAGGTGGCAAAGTTATAAGAGATGGAATGGGCCAGTCATCAGGGAATGAACCAGCTGACTCATTGGACACAGTTATAACTAATGCTGTAATCATTGATTATAGTGGGATCTTCAAGGCAGATATTGGTATTAAAGATGGTCATATAGTTGCCCTTGGGAAATCAGGCAATCCAGATGTTATGGATTGTGAACCTAGCAATATGATCATTGGGGTTAACACAGAGGTCATTGCAGCAGAAGGACAAATTGTGACTGCAGGAGCTATAGACTGTCATGTGCATTTCATATGCCCTCAGTTGGCATATGAAGCCATATCAAGTGGTATCACAACCCTAGTGGGAGGTGGAACAGGACCTGCTACGGGGACTCGTGCAACAACATGCACACCATCTCCATTGCAAATGAAGTTAATGCTGCAATCAACTGATGAAGTGCCTCTAAATTTTGGCTTTACAGGGAAGGGGAACAGTGCCGAACCTGATGGGCTATGCAAGATAATCAAGGCTGGAGCAATGGGTCTGAAGCTGCATGAAGACTGGGGCACAACTCCTGCTGCAATAGACAATTGTTTGAGTGTTGGAGACCAATATGACGTCCAGGTTAATATCCACACGGACACCTTGAATGAATCTGGATTTGTGGAACACACAATCGCTGCATTTAAAGACAGAACTATCCATACTTACCACAGTGAAGGTGCAGGTGGGGGTCATGCTCCAGATATTATCAAAGTATGTGGTGTGGAAAATGTCCTGCCCTCATCAACGAATCCCACACGGCCTTTCACCTCCAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTAGCTTTTGCTGAATCAAGGATAAGAGCTGAAACAATTGCCGCAGAAGATATATTGCATGATATGGGGGCAATTAGCATGGTAGCTTCTGATTCACAGGCTATGGGTCGCATTGGAGAGGTGATAACCAGAACTTGGCAAACTGCTGACAAGATGAAATCACAAAGAGGGTCAATAGAACCTAGTGGATCCAACAATGACAATTTCCGGATCAAGAGATACATTGCAAAGTACACCATAAATCCAGCAATAGCAAATGGATTTTCTAAATATGTTGGTTCAGTTCAGTTGGGCAAGTTCGCTGATCTTGTTCTATGGAAGCCATCTTTCTTTGGGGCAAAACCAGAAATGGTGGTCAAAGGTGGTGCAATTGCATGGGCTAATATGGGTGATCCAAACGCAAGCATCCCGACACCTGAACCGGTCATTTCAAGACCTATGTTTGGTGCATTTGGCAAGGCTAGTAGTGCTCATTCCATTGCCTTTGTCAGCAAGGAAGCTAAAAGAATTGGGGTTAAAGCCTTGTATGACCTGAACAAGAGAGTGGAAGCTGTGGGCAATGTTAGGAAGCTCCGCAAACGCGACATGGAGCTCAATGACGCCCTGCCAAATATTAAAGTTGACCCAGAGACATACACGGTGACAGCAGACGGCGAGGTTCTGACCTGCAGTGCAGCCACCACCGTTCCCCTATCCCGGAATTACTTCCTGTTTTAG

Protein Analysis

830

Amino Acids

89.32

Weight (kDa)

5.67

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urease_gamma PF00547 1 - 100 8.1e-42 Urease, gamma subunit
Urease_beta PF00699 133 - 225 1.2e-36 Urease beta subunit
Urease_alpha PF00449 264 - 382 1.4e-54 Urease alpha-subunit, N-terminal domain
Amidohydro_1 PF01979 388 - 714 2.5e-76 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 932, 992
AarI CACCTGC 1 cut(s) 1607
AatII GACGTC 1 cut(s) 1512
Acc36I ACCTGC 5 cut(s) 235, 589, 1279, 1607, 2450
AccB7I CCANNNNNTGG 1 cut(s) 1911
AccII CGCG 1 cut(s) 2358
AccIII TCCGGA 1 cut(s) 1983
AciI CCGC 2 cut(s) 1818, 2350
AclWI GGATC 6 cut(s) 287, 1031, 1959, 1972, 1994, 2162
AcsI RAATTY 1 cut(s) 1357
AcuI CTGAAG 2 cut(s) 120, 1457
AcyI GRCGYC 2 cut(s) 1509, 2376
AfaI GTAC 3 cut(s) 630, 681, 2009
AfiI CCNNNNNNNGG 7 cut(s) 830, 1213, 1277, 1759, 1911, 2219, 2472
AflIII ACRYGT 2 cut(s) 622, 813
AgeI ACCGGT 1 cut(s) 2197
AgsI TTSAA 5 cut(s) 224, 878, 1030, 1540, 2208
AjnI CCWGG 3 cut(s) 397, 534, 1512
AjuI GAANNNNNNNTTGG 2 cut(s) 897, 929
Alw21I GWGCWC 2 cut(s) 2248, 2370
Alw26I GTCTC 4 cut(s) 61, 474, 1491, 2399
AlwI GGATC 6 cut(s) 287, 1031, 1959, 1972, 1994, 2162
AlwNI CAGNNNCTG 1 cut(s) 1271
Aor13HI TCCGGA 1 cut(s) 1983
AoxI GGCC 5 cut(s) 34, 485, 820, 946, 1694
ApeKI GCWGC 7 cut(s) 773, 1148, 1330, 1441, 1469, 1571, 2450
ApoI RAATTY 1 cut(s) 1357
AseI ATTAAT 1 cut(s) 435
AsiGI ACCGGT 1 cut(s) 2197
Asp700I GAANNNNTTC 1 cut(s) 2480
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 5 cut(s) 34, 270, 821, 946, 1268
AsuC2I CCSGG 3 cut(s) 216, 609, 2473
AvaII GGWCC 2 cut(s) 270, 1268
BaeGI GKGCMC 1 cut(s) 1460
BamHI GGATCC 1 cut(s) 1964
BanII GRGCYC 1 cut(s) 2370
BauI CACGAG 3 cut(s) 75, 795, 1284
BbsI GAAGAC 1 cut(s) 1455
Bbv12I GWGCWC 2 cut(s) 2248, 2370
BbvI GCAGC 7 cut(s) 785, 1160, 1317, 1428, 1456, 1558, 2462
BceAI ACGGC 2 cut(s) 1709, 2443
BciT130I CCWGG 3 cut(s) 399, 536, 1514
BclI TGATCA 1 cut(s) 1119
BcnI CCSGG 3 cut(s) 216, 609, 2473
BcoDI GTCTC 4 cut(s) 61, 474, 1491, 2399
BfaI CTAG 8 cut(s) 56, 194, 326, 747, 1110, 1250, 1959, 2238
BfmI CTRYAG 4 cut(s) 1170, 1179, 1713, 2443
BfuAI ACCTGC 5 cut(s) 235, 589, 1279, 1607, 2450
BglII AGATCT 1 cut(s) 316
BisI GCNGC 8 cut(s) 774, 1149, 1331, 1442, 1470, 1572, 1818, 2451
BlsI GCNGC 8 cut(s) 775, 1150, 1332, 1443, 1471, 1573, 1819, 2452
Bme1390I CCNGG 6 cut(s) 216, 399, 536, 609, 1514, 2473
Bme18I GGWCC 2 cut(s) 270, 1268
BmgT120I GGNCC 5 cut(s) 34, 270, 821, 946, 1268
BmiI GGNNCC 3 cut(s) 12, 271, 1966
BmrFI CCNGG 6 cut(s) 216, 399, 536, 609, 1514, 2473
BmrI ACTGGG 1 cut(s) 1462
BmsI GCATC 3 cut(s) 760, 1732, 2190
BmuI ACTGGG 1 cut(s) 1462
BpiI GAAGAC 1 cut(s) 1455
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 3 cut(s) 498, 1443, 1617
BpuMI CCSGG 3 cut(s) 216, 609, 2473
BsaBI GATNNNNATC 1 cut(s) 1722
BsaHI GRCGYC 2 cut(s) 1509, 2376
BsaI GGTCTC 1 cut(s) 1491
BsaJI CCNNGG 2 cut(s) 608, 1069
BsaWI WCCGGW 2 cut(s) 1983, 2197
BsaXI ACNNNNNCTCC 2 cut(s) 1291, 1321
Bsc4I CCNNNNNNNGG 7 cut(s) 830, 1213, 1277, 1759, 1911, 2219, 2472
Bse118I RCCGGY 1 cut(s) 2197
Bse1I ACTGG 5 cut(s) 481, 488, 835, 949, 1457
Bse3DI GCAATG 6 cut(s) 1143, 1310, 1434, 1998, 2253, 2341
Bse8I GATNNNNATC 1 cut(s) 1722
BseAI TCCGGA 1 cut(s) 1983
BseBI CCWGG 3 cut(s) 399, 536, 1514
BseDI CCNNGG 2 cut(s) 608, 1069
BseGI GGATG 2 cut(s) 907, 2181
BseJI GATNNNNATC 1 cut(s) 1722
BseLI CCNNNNNNNGG 7 cut(s) 830, 1213, 1277, 1759, 1911, 2219, 2472
BseMI GCAATG 6 cut(s) 1143, 1310, 1434, 1998, 2253, 2341
BseMII CTCAG 2 cut(s) 137, 1223
BseNI ACTGG 5 cut(s) 481, 488, 835, 949, 1457
BseRI GAGGAG 1 cut(s) 666
BseSI GKGCMC 1 cut(s) 1460
BseXI GCAGC 7 cut(s) 785, 1160, 1317, 1428, 1456, 1558, 2462
BseYI CCCAGC 1 cut(s) 47
BsgI GTGCAG 4 cut(s) 263, 696, 1635, 2469
Bsh1236I CGCG 1 cut(s) 2358
BshFI GGCC 5 cut(s) 36, 487, 822, 948, 1696
BshTI ACCGGT 1 cut(s) 2197
BsiHKAI GWGCWC 2 cut(s) 2248, 2370
BsiSI CCGG 5 cut(s) 215, 608, 1984, 2198, 2473
BslFI GGGAC 3 cut(s) 268, 283, 1294
BslI CCNNNNNNNGG 7 cut(s) 830, 1213, 1277, 1759, 1911, 2219, 2472
BsmAI GTCTC 4 cut(s) 61, 474, 1491, 2399
BsmFI GGGAC 3 cut(s) 268, 283, 1294
BsnI GGCC 5 cut(s) 36, 487, 822, 948, 1696
Bso31I GGTCTC 1 cut(s) 1491
Bsp1286I GDGCHC 3 cut(s) 1460, 2248, 2370
Bsp13I TCCGGA 1 cut(s) 1983
Bsp143I GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
BspACI CCGC 2 cut(s) 1818, 2350
BspANI GGCC 5 cut(s) 36, 487, 822, 948, 1696
BspCNI CTCAG 2 cut(s) 136, 1222
BspEI TCCGGA 1 cut(s) 1983
BspFNI CGCG 1 cut(s) 2358
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 12, 271, 1966
BspMAI CTGCAG 2 cut(s) 1174, 2447
BspMI ACCTGC 5 cut(s) 235, 589, 1279, 1607, 2450
BspPI GGATC 6 cut(s) 287, 1031, 1959, 1972, 1994, 2162
BspTNI GGTCTC 1 cut(s) 1491
BsrDI GCAATG 6 cut(s) 1143, 1310, 1434, 1998, 2253, 2341
BsrFI RCCGGY 1 cut(s) 2197
BsrI ACTGG 5 cut(s) 481, 488, 835, 949, 1457
BssAI RCCGGY 1 cut(s) 2197
BssECI CCNNGG 2 cut(s) 608, 1069
BssMI GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
BssNI GRCGYC 2 cut(s) 1509, 2376
BssSI CACGAG 3 cut(s) 75, 795, 1284
BssT1I CCWWGG 1 cut(s) 1069
Bst2BI CACGAG 3 cut(s) 75, 795, 1284
Bst2UI CCWGG 3 cut(s) 399, 536, 1514
Bst4CI ACNGT 8 cut(s) 389, 787, 988, 1187, 1385, 1607, 2416, 2461
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 2 cut(s) 1509, 2376
BstC8I GCNNGC 1 cut(s) 2179
BstDEI CTNAG 2 cut(s) 123, 1209
BstENI CCTNNNNNAGG 1 cut(s) 1757
BstF5I GGATG 2 cut(s) 907, 2181
BstFNI CGCG 1 cut(s) 2358
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 9 cut(s) 295, 319, 547, 1026, 1122, 1967, 1989, 2083, 2170
BstMAI GTCTC 4 cut(s) 61, 474, 1491, 2399
BstMBI GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
BstMWI GCNNNNNNNGC 5 cut(s) 116, 590, 710, 1223, 2243
BstNI CCWGG 3 cut(s) 399, 536, 1514
BstNSI RCATGY 3 cut(s) 626, 817, 1299
BstSCI CCNGG 6 cut(s) 214, 397, 534, 607, 1512, 2471
BstSFI CTRYAG 4 cut(s) 1170, 1179, 1713, 2443
BstSLI GKGCMC 1 cut(s) 1460
BstUI CGCG 1 cut(s) 2358
BstV1I GCAGC 7 cut(s) 785, 1160, 1317, 1428, 1456, 1558, 2462
BstV2I GAAGAC 1 cut(s) 1455
BstX2I RGATCY 3 cut(s) 316, 1023, 1964
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 3 cut(s) 316, 1023, 1964
BsuRI GGCC 5 cut(s) 36, 487, 822, 948, 1696
BtsCI GGATG 2 cut(s) 907, 2181
BtsI GCAGTG 1 cut(s) 2452
BtsIMutI CAGTG 4 cut(s) 783, 1390, 1612, 2452
BveI ACCTGC 5 cut(s) 235, 589, 1279, 1607, 2450
Cac8I GCNNGC 1 cut(s) 2179
CaiI CAGNNNCTG 1 cut(s) 1271
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 1 cut(s) 2197
Cfr13I GGNCC 5 cut(s) 34, 270, 821, 946, 1268
CseI GACGC 1 cut(s) 2384
CsiI ACCWGGT 1 cut(s) 534
Csp6I GTAC 3 cut(s) 629, 680, 2008
CspAI ACCGGT 1 cut(s) 2197
CspCI CAANNNNNGTGG 4 cut(s) 1233, 1268, 1633, 1668
CviQI GTAC 3 cut(s) 629, 680, 2008
DdeI CTNAG 2 cut(s) 123, 1209
DpnI GATC 9 cut(s) 294, 318, 546, 1025, 1121, 1966, 1988, 2082, 2169
DpnII GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
DraI TTTAAA 2 cut(s) 333, 1579
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 2368
Eco130I CCWWGG 1 cut(s) 1069
Eco24I GRGCYC 1 cut(s) 2370
Eco31I GGTCTC 1 cut(s) 1491
Eco47I GGWCC 2 cut(s) 270, 1268
Eco53kI GAGCTC 1 cut(s) 2368
Eco57I CTGAAG 2 cut(s) 120, 1457
EcoICRI GAGCTC 1 cut(s) 2368
EcoNI CCTNNNNNAGG 1 cut(s) 1757
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRII CCWGG 3 cut(s) 397, 534, 1512
EcoT14I CCWWGG 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 2370
ErhI CCWWGG 1 cut(s) 1069
FaqI GGGAC 3 cut(s) 268, 283, 1294
FauNDI CATATG 4 cut(s) 559, 805, 1202, 1219
FbaI TGATCA 1 cut(s) 1119
Fnu4HI GCNGC 8 cut(s) 774, 1149, 1331, 1442, 1470, 1572, 1818, 2451
FokI GGATG 2 cut(s) 914, 2168
FriOI GRGCYC 1 cut(s) 2370
Fsp4HI GCNGC 8 cut(s) 774, 1149, 1331, 1442, 1470, 1572, 1818, 2451
FspBI CTAG 8 cut(s) 56, 194, 326, 747, 1110, 1250, 1959, 2238
GlaI GCGC 1 cut(s) 569
GluI GCNGC 8 cut(s) 774, 1149, 1331, 1442, 1470, 1572, 1818, 2451
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 3 cut(s) 498, 1443, 1617
HaeIII GGCC 5 cut(s) 36, 487, 822, 948, 1696
HapII CCGG 5 cut(s) 215, 608, 1984, 2198, 2473
HgaI GACGC 1 cut(s) 2384
HhaI GCGC 1 cut(s) 570
Hin1I GRCGYC 2 cut(s) 1509, 2376
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HincII GTYRAC 2 cut(s) 1132, 2398
HindII GTYRAC 2 cut(s) 1132, 2398
HinfI GANTC 9 cut(s) 645, 800, 905, 974, 1282, 1544, 1684, 1790, 1868
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 5 cut(s) 215, 608, 1984, 2198, 2473
Hpy166II GTNNAC 6 cut(s) 385, 620, 1106, 1132, 2010, 2398
Hpy188I TCNGA 6 cut(s) 126, 462, 650, 1437, 1867, 2439
Hpy8I GTNNAC 6 cut(s) 385, 620, 1106, 1132, 2010, 2398
HpyAV CCTTC 7 cut(s) 434, 438, 442, 746, 1148, 1369, 1604
HpyCH4III ACNGT 8 cut(s) 389, 787, 988, 1187, 1385, 1607, 2416, 2461
HpyCH4IV ACGT 2 cut(s) 257, 1509
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 590, 710, 1223, 2243
HpyF3I CTNAG 2 cut(s) 123, 1209
HpySE526I ACGT 2 cut(s) 257, 1509
Hsp92I GRCGYC 2 cut(s) 1509, 2376
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 1 cut(s) 1983
Ksp22I TGATCA 1 cut(s) 1119
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
LmnI GCTCC 6 cut(s) 701, 1175, 1424, 1636, 2352, 2365
Lsp1109I GCAGC 7 cut(s) 785, 1160, 1317, 1428, 1456, 1558, 2462
LweI GCATC 3 cut(s) 760, 1732, 2190
MabI ACCWGGT 1 cut(s) 534
MaeI CTAG 8 cut(s) 56, 194, 326, 747, 1110, 1250, 1959, 2238
MaeII ACGT 2 cut(s) 257, 1509
MaeIII GTNAC 4 cut(s) 149, 503, 1165, 2416
MalI GATC 9 cut(s) 294, 318, 546, 1025, 1121, 1966, 1988, 2082, 2169
MboI GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
MboII GAAGA 9 cut(s) 76, 203, 338, 743, 746, 1018, 1460, 1784, 1835
MfeI CAATTG 4 cut(s) 360, 1480, 1812, 2145
MflI RGATCY 3 cut(s) 316, 1023, 1964
MhlI GDGCHC 3 cut(s) 1460, 2248, 2370
MlyI GAGTC 4 cut(s) 654, 809, 968, 1276
MmeI TCCRAC 2 cut(s) 1474, 1992
MroI TCCGGA 1 cut(s) 1983
MroXI GAANNNNTTC 1 cut(s) 2480
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 5 cut(s) 215, 608, 1984, 2198, 2473
MspR9I CCNGG 6 cut(s) 216, 399, 536, 609, 1514, 2473
MunI CAATTG 4 cut(s) 360, 1480, 1812, 2145
MvaI CCWGG 3 cut(s) 399, 536, 1514
MvnI CGCG 1 cut(s) 2358
MwoI GCNNNNNNNGC 5 cut(s) 116, 590, 710, 1223, 2243
NciI CCSGG 3 cut(s) 216, 609, 2473
NdeI CATATG 4 cut(s) 559, 805, 1202, 1219
NdeII GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
NlaIV GGNNCC 3 cut(s) 12, 271, 1966
NmuCI GTSAC 4 cut(s) 149, 503, 1165, 2416
NspI RCATGY 3 cut(s) 626, 817, 1299
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 1607
PciI ACATGT 2 cut(s) 622, 813
PdmI GAANNNNTTC 1 cut(s) 2480
PfeI GAWTC 5 cut(s) 905, 1544, 1684, 1790, 1868
PflMI CCANNNNNTGG 1 cut(s) 1911
PfoI TCCNGGA 1 cut(s) 2471
PinAI ACCGGT 1 cut(s) 2197
PkrI GCNGC 8 cut(s) 775, 1150, 1332, 1443, 1471, 1573, 1819, 2452
PleI GAGTC 4 cut(s) 653, 808, 968, 1276
PpsI GAGTC 4 cut(s) 653, 808, 968, 1276
PpuMI RGGWCCY 1 cut(s) 1268
PscI ACATGT 2 cut(s) 622, 813
PshBI ATTAAT 1 cut(s) 435
PsiI TTATAA 2 cut(s) 932, 992
Psp124BI GAGCTC 1 cut(s) 2370
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 3 cut(s) 397, 534, 1512
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 3 cut(s) 397, 534, 1512
PspN4I GGNNCC 3 cut(s) 12, 271, 1966
PspPI GGNCC 5 cut(s) 34, 270, 821, 946, 1268
PspPPI RGGWCCY 1 cut(s) 1268
PsrI GAACNNNNNNTAC 2 cut(s) 1579, 1611
PstI CTGCAG 2 cut(s) 1174, 2447
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 3 cut(s) 316, 1023, 1964
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 3 cut(s) 630, 681, 2009
RsaNI GTAC 3 cut(s) 629, 680, 2008
SacI GAGCTC 1 cut(s) 2370
SatI GCNGC 8 cut(s) 774, 1149, 1331, 1442, 1470, 1572, 1818, 2451
Sau3AI GATC 9 cut(s) 292, 316, 544, 1023, 1119, 1964, 1986, 2080, 2167
Sau96I GGNCC 5 cut(s) 34, 270, 821, 946, 1268
SchI GAGTC 4 cut(s) 654, 809, 968, 1276
ScrFI CCNGG 6 cut(s) 216, 399, 536, 609, 1514, 2473
SduI GDGCHC 3 cut(s) 1460, 2248, 2370
SexAI ACCWGGT 1 cut(s) 534
SfaNI GCATC 3 cut(s) 760, 1732, 2190
SfcI CTRYAG 4 cut(s) 1170, 1179, 1713, 2443
SinI GGWCC 2 cut(s) 270, 1268
SsiI CCGC 2 cut(s) 1818, 2350
SspI AATATT 2 cut(s) 427, 2389
SspMI CTAG 8 cut(s) 56, 194, 326, 747, 1110, 1250, 1959, 2238
SstI GAGCTC 1 cut(s) 2370
StyD4I CCNGG 6 cut(s) 214, 397, 534, 607, 1512, 2471
StyI CCWWGG 1 cut(s) 1069
TaaI ACNGT 8 cut(s) 389, 787, 988, 1187, 1385, 1607, 2416, 2461
TaiI ACGT 2 cut(s) 260, 1512
TaqI TCGA 2 cut(s) 249, 547
TatI WGTACW 1 cut(s) 2007
TauI GCSGC 1 cut(s) 1820
TfiI GAWTC 5 cut(s) 905, 1544, 1684, 1790, 1868
TscAI CASTG 4 cut(s) 790, 1390, 1612, 2452
TseFI GTSAC 4 cut(s) 149, 503, 1165, 2416
TseI GCWGC 7 cut(s) 773, 1148, 1330, 1441, 1469, 1571, 2450
Tsp45I GTSAC 4 cut(s) 149, 503, 1165, 2416
TspGWI ACGGA 1 cut(s) 1544
TspRI CASTG 4 cut(s) 790, 1390, 1612, 2452
Van91I CCANNNNNTGG 1 cut(s) 1911
VpaK11BI GGWCC 2 cut(s) 270, 1268
VspI ATTAAT 1 cut(s) 435
XagI CCTNNNNNAGG 1 cut(s) 1757
XapI RAATTY 1 cut(s) 1357
XceI RCATGY 3 cut(s) 626, 817, 1299
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 2480
XspI CTAG 8 cut(s) 56, 194, 326, 747, 1110, 1250, 1959, 2238
ZraI GACGTC 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.