Rorug02G0437300
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000287
Physical Location & Seq
Forward (+)
55864923 .. 55874131
9209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0437300.1

Sequence Viewer

Length: 2757 bp
ATGGTCTCTGGGTCGCGAATCGAAGGTGGTGGGACTCAGATAATCTCCGCCGGAGTGAGGAAAACCATCCAATCCATCAAAGAAATAGTGGGCAATCACTCTGATATTGATATCTATTTGGCTCTCAAAGAGACCGATATGGATCCTAATGAAACTGCCCAGAAATTACTCAACCAAGACCCATTTCATGAGGTGAAGAGAAAAAGAGACAAAAAGAAGGACGTGTCACCTAAGAGTATCGGGCAAAAGGTTCCTACCGAGCCGAGAAGACATTTTGAGAGTGCTGGTCAAGGACCAAGATCAAATACAATTTCGGATCGTGTTGTGGAGCCTAAAAGACATTTTGAGAGTGCAGGTCAAGGACCTAGATCAAATACAATTTCTGATCGTAATGTCGAGCCTAGAAGACATTTTGACAGTGCAGGTCAAGGACCAAAATCTAATTCATTTTCTGATCGAAATGTTGAGCCAAGAAGACATTTTGAGAGTGCAGGTCAAGGACCAAGACATAATTCATTTTCTGATCGTAGTGTTAGAAGAGGAGGTTATGTGCGCAGGGGTTTACCTGGAATCAGCAGAGAGTTTCGTGTTGTGAGAGACAACAGAGCTAACCAAAATATGGATGGAGAAGTTAAGCCTGCTTCACCTCAATGTACTACATCCACCAACGAGCAAGCGATTTCAAATGTTTCTGAGAAGGGCCAAACAGGAAATTCAAGCAGTCAAAAGCCATCTAATAGACAGCATTCCTCACAAGCATTGAATGGGCAAACTGATTCTCGAATTAGAACGAACGTTGCTAATTCAACTGGTACGATTAGAAAGGAGACATTAGTGGAGAAGCGAGTTGCTCTTCCAAATTCAGTTTCACGGGTACAAGCAGGGAAACCAAACAACTCCCAACCAAATTCTGCATCAAGCACTTCTGTTATTGGGGTGTATTCCTCGTCCACAGATCCTGTTCATGTGCCGTCTCCTGATTCTAGACTATCTGCTTCTGTTGGTGCAATTAAGCGCGAAGTTGGGGTGGTTGGTGTTCGGAAACAGTCTTCTGACAACTCCAAGTCAGCTGTACCAAGTAGTTCTTTCTCTAATTCACTTTTGGGAAAAGAGGGCTCTGCAGAATCATTTAGATCTTTCACTGGTATCTCTAAACCTGATCAACTCAGCCAAACTTCTGAATCTACGATGCCTAGCATGCCGGTCAGCAGATCTTTCGTAAGTAATCAGCACAATGTCAGGGCGCATCAACAACCTGTGGGTCATCAGAAAGATGCAGCTTCCCAGCCTAACAAGGAGTGGAAACCTAAATCAAGTCAGAAGCCAAGCCCTAATAATCCTGGAGTCATTGGAACACCAACAAAATCTGCTTCTCCTCTTTCTGATGATTCAAAGGTCTCAGAGTCAGAAGCGGCTAAGTTGCAAGATAAGCTTGCCCGAGTAAATATATATGAGAACTGTAATGTTGTGATAGCGCAAAATATTAGAGTCCCTGAGAGTGACCGTTTTCGGCTAACCTTTGGAAGCTTGGGGACAGAGTTTGATTCAACAGGAAACACAGTTAACGGATTTCAAGCTGGAGCTGCGGAGGAGTTGAACAGGGAACCTCCAGCAAGTTTGTCAACATCAGTTCCCGAGTCGCGTGGTGATGAGGCTTCCGGCACGAAGCCAGTAGATTTATTAGATGACCAAGTTAGAAATTCTGGATCTGATTTTTCGGCACCGTCTGCAGTGCCTGAGCATTTGCCTGAGAAAAAAGAGACCTCAAGTCCTCAGAGTTTGGACAATTATGCAGATATTGGTTTGGTTCGAGACAACAGTCCATCCTTTGCACCTTCAGATTCGCAGCACCAAGATCCACCAGAGTTACAAGGTTTTACGGCATTTGATCCTCAGACTGGTTATGATATACCTTATTACAGACCCAGTATGGATGAAAGTGTTCGTGGACAGGGTCTACCATCTCCTCAGGAGTCTTTGAGTTCACATAATGCCAACAGTATTCCTGCATCAACAGTTGCCATGGTACAACAGCAACCACCGCATGTGGCGCAGATGTACCCACAAGTTCATGTTTCACATTATGCTAATCTTATGCCATATCGTCAGTTTATCTCACCAGTATATGTTCCACCAATGGCTGTGCCCGGCTATTCCAATAATCCTGCCTATCCTCACATGTCTAATGGCAACAGCTACTTGCTGATGCCTGGAGGTGGTTCCCACCTAAATGCAAACAGCCTCAAGTATGGAGTTCAGCAATTTAAGCCCGTCCCTGCTGGAAGTCCCACTGGGTTTGGAAATTTTACTAACCCAGCAGGTTATGCAATGAATGCCCCTGGTGTAGTTGGAGGTGCCACTGGACTCGAAGATTCATCAAGAATGAAGTACAAAGATGGCAATCTTTATGTTCCAAATCCACAGGCGGAGACATCAGAGATTTGGATTCAGAACCCAAGGGAGCATCCAGGAATGCAATCCACTCCATACTACAACATGCCTGGGCAAACACCTCATGCTCCTTATATGCCGTCCCACGCCGGCCACGCTTCCTTTAATGCAGCTGCAGCACAATCTTCGCACATGCAATTCCCAGGAATGTACCATCCTCCTCAGCCAGCTGCAATGGCCAGTCCGCACCATATGGGCCCTGCTATGCCTGGCAATGTCGGAGTGGGGGTGGCCGCAGCTGCTCCTGGGGCACAGGCGTATCAGCAGCCCCAGTTGAATCATATGAACTGGACAACAAACTTCTGA

Protein Analysis

918

Amino Acids

98.94

Weight (kDa)

9.01

Isoelectric Point (pI)

56.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 15 - 73 5.3e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000287)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20410 FvH4_1g20410 FvH4_6g39250 FvH4_6g39250
malus_domestica MD00G1036800.v1.1 MD05G1042600.v1.1 MD10G1049900.v1.1
prunus_persica Prupe.6G191500_v2.0.a1 Prupe.8G064300_v2.0.a1 Prupe.8G064300_v2.0.a1 Prupe.8G064400_v2.0.a1
pyrus_communis pycom05g03520 pycom10g03500 pycom10g03510 pycom15g28250
rosa_chinensis RchiOBHm_Chr1g0336561 RchiOBHm_Chr2g0112551 RchiOBHm_Chr2g0112561 RchiOBHm_Chr2g0112631 RchiOBHm_Chr2g0112641 RchiOBHm_Chr2g0153091 RchiOBHm_Chr2g0153111 RchiOBHm_Chr2g0153141 RchiOBHm_Chr2g0153161 RchiOBHm_Chr2g0153171
rosa_laevigata RLG00000017991 RLG00000017992 RLG00000017995 RLG00000017997 RLG00000020676 RLG00000020677 RLG00000020678 RLG00000020682
rosa_multiflora Rmu_co8208234.1_g000001 Rmu_co8218328.1_g000001 Rmu_co8425243.1_g000001 Rmu_sc0000218.1_g000037 Rmu_sc0000218.1_g000044 Rmu_sc0000218.1_g000047 Rmu_sc0000218.1_g000052 Rmu_sc0001155.1_g000011 Rmu_sc0001155.1_g000026 Rmu_sc0001155.1_g000029 Rmu_sc0001499.1_g000001 Rmu_sc0001499.1_g000040 Rmu_sc0002401.1_g000032 Rmu_sc0002401.1_g000033 Rmu_sc0004070.1_g000003 Rmu_sc0004070.1_g000004 Rmu_sc0005547.1_g000001 Rmu_sc0006062.1_g000002 Rmu_sc0009780.1_g000005 Rmu_sc0013071.1_g000001 Rmu_sc0013071.1_g000012
rosa_roxburghii Rroxscaffold_2G00095130 Rroxscaffold_2G00095140 Rroxscaffold_2G00095160 Rroxscaffold_2G00095180 Rroxscaffold_2G00095190 Rroxscaffold_2G00095200 Rroxscaffold_2G00131290 Rroxscaffold_2G00131320 Rroxscaffold_2G00131350
rosa_rugosa Rorug02G0181500 Rorug02G0181500 Rorug02G0181600 Rorug02G0181700 Rorug02G0437300 Rorug02G0437400
rosa_samantha Rh1DG154100 Rh2AG235200 Rh2AG235300 Rh2AG235400 Rh2AG235600 Rh2AG235800 Rh2AG499700 Rh2AG499800 Rh2AG500000 Rh2AG500100 Rh2AG500200 Rh2BG248500 Rh2BG248700 Rh2BG248800 Rh2BG511000 Rh2BG511200 Rh2BG511300 Rh2BG511600 Rh2CG239600 Rh2CG239700 Rh2CG239900 Rh2CG240000 Rh2CG485900 Rh2CG486000 Rh2CG486200 Rh2CG486300 Rh2CG486400 Rh2DG242500 Rh2DG242600 Rh2DG242700 Rh2DG242900 Rh2DG243100 Rh2DG522800 Rh2DG523100 Rh2DG523200 Rh2DG523500 Rh2DG523600 Rh5DG200200
rosa_wichuraiana Rw2G018200 Rw2G018210 Rw2G018230 Rw2G018240 Rw2G041010 Rw2G041020 Rw2G041030 Rw2G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 554
Acc36I ACCTGC 4 cut(s) 344, 413, 482, 2309
AccB1I GGYRCC 2 cut(s) 1720, 2354
AccB7I CCANNNNNTGG 1 cut(s) 619
AccI GTMKAC 1 cut(s) 1957
AccII CGCG 3 cut(s) 16, 1017, 1642
AciI CCGC 7 cut(s) 48, 1412, 1586, 2042, 2426, 2636, 2685
AclI AACGTT 1 cut(s) 795
AclWI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
AcoI YGGCCR 3 cut(s) 2542, 2628, 2682
AcsI RAATTY 5 cut(s) 712, 859, 907, 1699, 2302
AcuI CTGAAG 1 cut(s) 1821
AfaI GTAC 8 cut(s) 655, 814, 876, 1074, 2028, 2060, 2390, 2603
AfiI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
AflIII ACRYGT 2 cut(s) 222, 2178
AgsI TTSAA 9 cut(s) 684, 717, 763, 807, 1392, 1548, 1574, 1597, 2728
AhdI GACNNNNNGTC 1 cut(s) 1767
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 9 cut(s) 565, 1339, 2209, 2338, 2467, 2500, 2593, 2659, 2695
AjuI GAANNNNNNNTTGG 8 cut(s) 168, 200, 427, 459, 496, 528, 850, 882
AlwI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
AlwNI CAGNNNCTG 2 cut(s) 959, 1736
Ama87I CYCGRG 2 cut(s) 1437, 1634
AoxI GGCC 5 cut(s) 700, 2542, 2628, 2647, 2682
ApaI GGGCCC 1 cut(s) 2651
ApoI RAATTY 5 cut(s) 712, 859, 907, 1699, 2302
Asp700I GAANNNNTTC 1 cut(s) 1941
AspLEI GCGC 5 cut(s) 555, 1017, 1246, 1477, 2053
AspS9I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
AsuC2I CCSGG 1 cut(s) 2148
AsuHPI GGTGA 5 cut(s) 205, 219, 636, 1658, 2109
AvaI CYCGRG 2 cut(s) 1437, 1634
AvaII GGWCC 4 cut(s) 293, 362, 431, 500
AxyI CCTNAGG 1 cut(s) 1968
BaeGI GKGCMC 3 cut(s) 2148, 2651, 2704
BaeI ACNNNNGTAYC 2 cut(s) 2693, 2726
BalI TGGCCA 1 cut(s) 2630
BamHI GGATCC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 1720, 2354
BanII GRGCYC 2 cut(s) 1118, 2651
BbsI GAAGAC 4 cut(s) 274, 412, 481, 1041
BbvCI CCTCAGC 1 cut(s) 2613
BccI CCATC 8 cut(s) 74, 83, 617, 739, 1831, 1969, 2390, 2613
BceAI ACGGC 3 cut(s) 955, 1896, 2515
BcgI CGANNNNNNTGC 4 cut(s) 1198, 1232, 2559, 2593
BciT130I CCWGG 9 cut(s) 567, 1341, 2211, 2340, 2469, 2502, 2595, 2661, 2697
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 2148
BfaI CTAG 4 cut(s) 366, 402, 984, 1194
BfmI CTRYAG 3 cut(s) 1119, 1728, 2565
BfuAI ACCTGC 4 cut(s) 344, 413, 482, 2309
BglII AGATCT 2 cut(s) 1133, 1211
Bme18I GGWCC 4 cut(s) 293, 362, 431, 500
BmeRI GACNNNNNGTC 1 cut(s) 1767
BmeT110I CYCGRG 2 cut(s) 1437, 1634
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
BmiI GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
BmrI ACTGGG 3 cut(s) 1920, 2301, 2716
BmsI GCATC 7 cut(s) 923, 1179, 1255, 1264, 2018, 2196, 2473
BmuI ACTGGG 3 cut(s) 1920, 2301, 2716
BoxI GACNNNNGTC 1 cut(s) 1818
BpiI GAAGAC 4 cut(s) 274, 412, 481, 1041
BpmI CTGGAG 4 cut(s) 1362, 1593, 1599, 2232
Bpu10I CCTNAGC 2 cut(s) 1737, 2613
BpuEI CTTGAG 2 cut(s) 1750, 2228
BpuMI CCSGG 1 cut(s) 2148
BsaBI GATNNNNATC 2 cut(s) 141, 2400
BsaI GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsaJI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 2664, 2694
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
Bse118I RCCGGY 2 cut(s) 1201, 2540
Bse21I CCTNAGG 1 cut(s) 1968
Bse3DI GCAATG 3 cut(s) 2334, 2631, 2671
Bse8I GATNNNNATC 2 cut(s) 141, 2400
BseBI CCWGG 9 cut(s) 567, 1341, 2211, 2340, 2469, 2502, 2595, 2661, 2697
BseDI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BseGI GGATG 7 cut(s) 66, 628, 659, 1823, 1939, 2464, 2605
BseJI GATNNNNATC 2 cut(s) 141, 2400
BseLI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
BseMI GCAATG 3 cut(s) 2334, 2631, 2671
BseRI GAGGAG 5 cut(s) 555, 1365, 1604, 1956, 2601
BseSI GKGCMC 3 cut(s) 2148, 2651, 2704
BseYI CCCAGC 2 cut(s) 1284, 2314
BsgI GTGCAG 3 cut(s) 372, 441, 510
Bsh1236I CGCG 3 cut(s) 16, 1017, 1642
BshFI GGCC 5 cut(s) 702, 2544, 2630, 2649, 2684
BshNI GGYRCC 2 cut(s) 1720, 2354
BsiHKCI CYCGRG 2 cut(s) 1437, 1634
BsiSI CCGG 5 cut(s) 51, 1202, 1659, 2148, 2541
BslFI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
BslI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
BsmBI CGTCTC 1 cut(s) 978
BsmFI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
BsmI GAATGC 3 cut(s) 745, 2338, 2478
BsnI GGCC 5 cut(s) 702, 2544, 2630, 2649, 2684
Bso31I GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsoBI CYCGRG 2 cut(s) 1437, 1634
Bsp120I GGGCCC 1 cut(s) 2647
Bsp1286I GDGCHC 4 cut(s) 1118, 2148, 2651, 2704
Bsp19I CCATGG 1 cut(s) 2022
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 7 cut(s) 48, 1412, 1586, 2042, 2426, 2636, 2685
BspANI GGCC 5 cut(s) 702, 2544, 2630, 2649, 2684
BspFNI CGCG 3 cut(s) 16, 1017, 1642
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
BspMAI CTGCAG 3 cut(s) 1123, 1732, 2569
BspMI ACCTGC 4 cut(s) 344, 413, 482, 2309
BspPI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 1720, 2354
BspTNI GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsrDI GCAATG 3 cut(s) 2334, 2631, 2671
BsrFI RCCGGY 2 cut(s) 1201, 2540
BssAI RCCGGY 2 cut(s) 1201, 2540
BssECI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BssT1I CCWWGG 2 cut(s) 2022, 2456
Bst2UI CCWGG 9 cut(s) 567, 1341, 2211, 2340, 2469, 2502, 2595, 2661, 2697
Bst4CI ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
Bst6I CTCTTC 3 cut(s) 191, 532, 858
BstAPI GCANNNNNTGC 3 cut(s) 1727, 2324, 2333
BstC8I GCNNGC 6 cut(s) 639, 675, 1199, 1434, 2542, 2619
BstDSI CCRYGG 1 cut(s) 2022
BstF5I GGATG 7 cut(s) 66, 628, 659, 1823, 1939, 2464, 2605
BstFNI CGCG 3 cut(s) 16, 1017, 1642
BstHHI GCGC 5 cut(s) 555, 1017, 1246, 1477, 2053
BstNI CCWGG 9 cut(s) 567, 1341, 2211, 2340, 2469, 2502, 2595, 2661, 2697
BstNSI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
BstPAI GACNNNNGTC 1 cut(s) 1818
BstSFI CTRYAG 3 cut(s) 1119, 1728, 2565
BstSLI GKGCMC 3 cut(s) 2148, 2651, 2704
BstUI CGCG 3 cut(s) 16, 1017, 1642
BstV2I GAAGAC 4 cut(s) 274, 412, 481, 1041
BstX2I RGATCY 6 cut(s) 142, 955, 1133, 1211, 1706, 1855
BstYI RGATCY 6 cut(s) 142, 955, 1133, 1211, 1706, 1855
Bsu36I CCTNAGG 1 cut(s) 1968
BsuRI GGCC 5 cut(s) 702, 2544, 2630, 2649, 2684
BtgI CCRYGG 1 cut(s) 2022
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 7 cut(s) 66, 628, 659, 1823, 1939, 2464, 2605
BtsI GCAGTG 1 cut(s) 1737
BtsIMutI CAGTG 5 cut(s) 424, 1140, 1737, 2289, 2358
BtuMI TCGCGA 1 cut(s) 16
BveI ACCTGC 4 cut(s) 344, 413, 482, 2309
Cac8I GCNNGC 6 cut(s) 639, 675, 1199, 1434, 2542, 2619
CaiI CAGNNNCTG 2 cut(s) 959, 1736
CciI TCATGA 1 cut(s) 187
CfoI GCGC 5 cut(s) 555, 1017, 1246, 1477, 2053
Cfr10I RCCGGY 2 cut(s) 1201, 2540
Cfr13I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
Csp6I GTAC 8 cut(s) 654, 813, 875, 1073, 2027, 2059, 2389, 2602
CspCI CAANNNNNGTGG 2 cut(s) 2278, 2313
CviQI GTAC 8 cut(s) 654, 813, 875, 1073, 2027, 2059, 2389, 2602
DriI GACNNNNNGTC 1 cut(s) 1767
EaeI YGGCCR 3 cut(s) 2542, 2628, 2682
Eam1104I CTCTTC 3 cut(s) 191, 532, 858
Eam1105I GACNNNNNGTC 1 cut(s) 1767
EarI CTCTTC 3 cut(s) 191, 532, 858
EciI GGCGGA 2 cut(s) 37, 2441
Eco130I CCWWGG 2 cut(s) 2022, 2456
Eco24I GRGCYC 2 cut(s) 1118, 2651
Eco31I GGTCTC 4 cut(s) 10, 125, 1402, 1754
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 4 cut(s) 293, 362, 431, 500
Eco57I CTGAAG 1 cut(s) 1821
Eco81I CCTNAGG 1 cut(s) 1968
Eco88I CYCGRG 2 cut(s) 1437, 1634
EcoO109I RGGNCCY 2 cut(s) 362, 2648
EcoRII CCWGG 9 cut(s) 565, 1339, 2209, 2338, 2467, 2500, 2593, 2659, 2695
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 2022, 2456
EcoT38I GRGCYC 2 cut(s) 1118, 2651
ErhI CCWWGG 2 cut(s) 2022, 2456
Esp3I CGTCTC 1 cut(s) 978
FalI AAGNNNNNCTT 4 cut(s) 1069, 1101, 1416, 1448
FaqI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
FauNDI CATATG 2 cut(s) 2643, 2733
FbaI TGATCA 1 cut(s) 1159
FblI GTMKAC 1 cut(s) 1957
FokI GGATG 7 cut(s) 53, 635, 646, 1810, 1946, 2451, 2592
FriOI GRGCYC 2 cut(s) 1118, 2651
FspBI CTAG 4 cut(s) 366, 402, 984, 1194
FspI TGCGCA 1 cut(s) 554
GlaI GCGC 5 cut(s) 554, 1016, 1245, 1476, 2052
GsaI CCCAGC 2 cut(s) 1288, 2318
GsuI CTGGAG 4 cut(s) 1362, 1593, 1599, 2232
HaeIII GGCC 5 cut(s) 702, 2544, 2630, 2649, 2684
HapII CCGG 5 cut(s) 51, 1202, 1659, 2148, 2541
HhaI GCGC 5 cut(s) 555, 1017, 1246, 1477, 2053
Hin6I GCGC 5 cut(s) 553, 1015, 1244, 1475, 2051
HinP1I GCGC 5 cut(s) 553, 1015, 1244, 1475, 2051
HincII GTYRAC 2 cut(s) 1564, 1623
HindII GTYRAC 2 cut(s) 1564, 1623
HindIII AAGCTT 2 cut(s) 1430, 1525
HpaI GTTAAC 1 cut(s) 1564
HpaII CCGG 5 cut(s) 51, 1202, 1659, 2148, 2541
HphI GGTGA 5 cut(s) 205, 219, 636, 1658, 2109
Hpy166II GTNNAC 7 cut(s) 563, 951, 1564, 1623, 1949, 1958, 1985
Hpy8I GTNNAC 7 cut(s) 563, 951, 1564, 1623, 1949, 1958, 1985
HpyAV CCTTC 4 cut(s) 17, 211, 691, 1845
HpyCH4III ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
HpyCH4IV ACGT 2 cut(s) 222, 795
HpySE526I ACGT 2 cut(s) 222, 795
HspAI GCGC 5 cut(s) 553, 1015, 1244, 1475, 2051
KroI GCCGGC 1 cut(s) 2540
KroNI GCCGGC 1 cut(s) 2542
Ksp22I TGATCA 1 cut(s) 1159
KspAI GTTAAC 1 cut(s) 1564
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 5 cut(s) 328, 1580, 2461, 2524, 2698
LweI GCATC 7 cut(s) 923, 1179, 1255, 1264, 2018, 2196, 2473
MaeI CTAG 4 cut(s) 366, 402, 984, 1194
MaeII ACGT 2 cut(s) 222, 795
MaeIII GTNAC 3 cut(s) 225, 1499, 1866
MboII GAAGA 9 cut(s) 208, 279, 417, 486, 549, 845, 1041, 2381, 2568
MflI RGATCY 6 cut(s) 142, 955, 1133, 1211, 1706, 1855
MhlI GDGCHC 4 cut(s) 1118, 2148, 2651, 2704
MlsI TGGCCA 1 cut(s) 2630
MluNI TGGCCA 1 cut(s) 2630
MlyI GAGTC 7 cut(s) 28, 1353, 1412, 1497, 1646, 1982, 2358
MmeI TCCRAC 2 cut(s) 2329, 2650
Mox20I TGGCCA 1 cut(s) 2630
MroNI GCCGGC 1 cut(s) 2540
MroXI GAANNNNTTC 1 cut(s) 1941
MscI TGGCCA 1 cut(s) 2630
MseI TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
MslI CAYNNNNRTG 2 cut(s) 649, 2229
Msp20I TGGCCA 1 cut(s) 2630
MspA1I CMGCKG 4 cut(s) 1070, 2564, 2621, 2690
MspI CCGG 5 cut(s) 51, 1202, 1659, 2148, 2541
Mva1269I GAATGC 3 cut(s) 745, 2338, 2478
MvaI CCWGG 9 cut(s) 567, 1341, 2211, 2340, 2469, 2502, 2595, 2661, 2697
MvnI CGCG 3 cut(s) 16, 1017, 1642
NaeI GCCGGC 1 cut(s) 2542
NciI CCSGG 1 cut(s) 2148
NcoI CCATGG 1 cut(s) 2022
NdeI CATATG 2 cut(s) 2643, 2733
NgoMIV GCCGGC 1 cut(s) 2540
NlaIV GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 2 cut(s) 225, 1499
NruI TCGCGA 1 cut(s) 16
NsbI TGCGCA 1 cut(s) 554
NspI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
PaeI GCATGC 1 cut(s) 1201
PagI TCATGA 1 cut(s) 187
PciI ACATGT 1 cut(s) 2178
PciSI GCTCTTC 1 cut(s) 858
PctI GAATGC 3 cut(s) 745, 2338, 2478
PdiI GCCGGC 1 cut(s) 2542
PdmI GAANNNNTTC 1 cut(s) 1941
PflFI GACNNNGTC 1 cut(s) 1953
PflMI CCANNNNNTGG 1 cut(s) 619
PfoI TCCNGGA 2 cut(s) 1339, 2467
PleI GAGTC 7 cut(s) 28, 1352, 1411, 1496, 1645, 1981, 2358
PpsI GAGTC 7 cut(s) 28, 1352, 1411, 1496, 1645, 1981, 2358
PpuMI RGGWCCY 1 cut(s) 362
PscI ACATGT 1 cut(s) 2178
PshAI GACNNNNGTC 1 cut(s) 1818
Psp1406I AACGTT 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 9 cut(s) 565, 1339, 2209, 2338, 2467, 2500, 2593, 2659, 2695
PspFI CCCAGC 2 cut(s) 1284, 2314
PspGI CCWGG 9 cut(s) 565, 1339, 2209, 2338, 2467, 2500, 2593, 2659, 2695
PspN4I GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
PspOMI GGGCCC 1 cut(s) 2647
PspPI GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
PspPPI RGGWCCY 1 cut(s) 362
PstI CTGCAG 3 cut(s) 1123, 1732, 2569
PstNI CAGNNNCTG 2 cut(s) 959, 1736
PsuI RGATCY 6 cut(s) 142, 955, 1133, 1211, 1706, 1855
PsyI GACNNNGTC 1 cut(s) 1953
PvuII CAGCTG 4 cut(s) 1070, 2564, 2621, 2690
RruI TCGCGA 1 cut(s) 16
RsaI GTAC 8 cut(s) 655, 814, 876, 1074, 2028, 2060, 2390, 2603
RsaNI GTAC 8 cut(s) 654, 813, 875, 1073, 2027, 2059, 2389, 2602
RseI CAYNNNNRTG 2 cut(s) 649, 2229
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
Sau96I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
SchI GAGTC 7 cut(s) 28, 1353, 1412, 1497, 1646, 1982, 2358
SduI GDGCHC 4 cut(s) 1118, 2148, 2651, 2704
SfaNI GCATC 7 cut(s) 923, 1179, 1255, 1264, 2018, 2196, 2473
SfcI CTRYAG 3 cut(s) 1119, 1728, 2565
SinI GGWCC 4 cut(s) 293, 362, 431, 500
SmiMI CAYNNNNRTG 2 cut(s) 649, 2229
SmlI CTYRAG 2 cut(s) 1765, 2243
SmoI CTYRAG 2 cut(s) 1765, 2243
SphI GCATGC 1 cut(s) 1201
SsiI CCGC 7 cut(s) 48, 1412, 1586, 2042, 2426, 2636, 2685
SspI AATATT 1 cut(s) 1483
SspMI CTAG 4 cut(s) 366, 402, 984, 1194
StyI CCWWGG 2 cut(s) 2022, 2456
TaaI ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
TaiI ACGT 2 cut(s) 225, 798
TaqI TCGA 6 cut(s) 21, 396, 457, 781, 1810, 2367
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 2 cut(s) 653, 2388
TauI GCSGC 2 cut(s) 1415, 2687
Tru1I TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
Tru9I TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
TscAI CASTG 5 cut(s) 424, 1147, 1737, 2296, 2365
TseFI GTSAC 2 cut(s) 225, 1499
Tsp45I GTSAC 2 cut(s) 225, 1499
TspGWI ACGGA 1 cut(s) 1581
TspRI CASTG 5 cut(s) 424, 1147, 1737, 2296, 2365
Tth111I GACNNNGTC 1 cut(s) 1953
Van91I CCANNNNNTGG 1 cut(s) 619
VpaK11BI GGWCC 4 cut(s) 293, 362, 431, 500
XapI RAATTY 5 cut(s) 712, 859, 907, 1699, 2302
XbaI TCTAGA 1 cut(s) 983
XceI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 1957
XmnI GAANNNNTTC 1 cut(s) 1941
XspI CTAG 4 cut(s) 366, 402, 984, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.