Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0000287

Physical Location & Seq

Genomic Coordinates Forward (+)

4076512 .. 4080884

Gene Structure 4373 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03520.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGCTTCTACTATTAATTTCAATGTCCACCACAAAGCTACTGAGCATGAATATGGTTCAGCAGCACTGGAGGTTGAAACACAAGTTAATAATATTGATCTAGCAGTACTGCCAAGAGCTGGCGGAGGAGGAGGTGCCGGAATCCAAAACAACAACCATGCAAAGATATTGGAGGAGGGTGTTTTGCTGACATGGGAGGACGTGTGGGTGACAGTAGTTTCCAAGAAAAATAACAGTATTGGTAGCAGCAGCAGATCAATCCTGCAAGGTCTAACCGGTTATGCCAAGCCTGGGGAGCTCTTGGCTATAATGGGCCCTTCTGGCTGTGGCAAGTCCACTCTTCTTGATGCTTTAGGAGGGAGACTGAATTCAAACACAAGGCAAACAGGGAAGATCCTAATCAATGGGCATAAACAATCACTGGCTTATGGAACTTCGGCATATGTCACACAAGATGATACTTTAATGACGACATTAACCGTTAAAGAAGCAGTATATTACTCTGCTCAGCTGCAACTGCCAGACTCCATGACCAAGTCAGAGAAGAAAGAGAGAGCAGAAGTTACAATTAGAGAGATGGGTTTGCAGGACGCCATGAACACAAGGATTGGAGGTTGGGGAGTTAAAGGCATAAGTGGTGGCCAAAAGAGAAGAGTCAGCATTTGCATTGAGCTTCTCACACGGCCTAAACTTCTATTCCTCGACGAACCGACAAGCGGCCTTGACAGTGCAGCTTCTTATTATGTCATGAGCAGAATTGCAAATCTTGATAAAGGTGATGGAACTCCAAGGACTATTGTTACATCCATCCATCAGCCTAGCTCTGAAGTCTTTCAACTTTTCAATAGTCTTTGTCTGCTGTCTTCTGGAAGAACTGTTTATTTTGGTCCTGCTTCTGCAGCAAATCAGTTTTTCACTTTAACTGGTTTCCCATGCCCAACTCTCCAAAATCCATCGGATCACTTCCTTAAGACCATAAACAAAGATTTTGAGCAGGACATTGAGCAAGGCGTAACCACACCAACAGAAATAGCAATTGATACTCTAATAAGATCCTACAAAGAATCTGAAACCTATCAGCAAGTTCACATACAAGTAGCTGAACTATGTAAACAGGAATTCGGTGAAAAATTGGAGAAGAGAAGCCATGCTGGCTTTCTTACACAATGTCTCGTACTTACAAAAAGATCATTCGTGAACATGCATAGAGATCGAGGCTACTATTGGTTGCGCCTGGCTATATACATCTTCATGACTCTAGGCCTCGGTACTGTCTATCATGAGCTTGGACATGGTTATAGATCCATTCAGGCAAGAGGCTCGCTGCTCATGTTCATATCTACTTTTCTAACTTTCATGGCCATTGGTGGATTCCCTTCCTTTGTGGAAGACATGAAGGTATTTGAAAGAGAAAGACTAAACGGGCACTACGGTGTCTGTGCATTTGCTTTTGGCAACACATTTTCTTCTGTTCCTTTCTTGGTAATGATTTCTTTAATTCCCGGGGCAATAGTTTATTACCTTGCTGGACTTCAAACCGGATTCGAACACTTTGTTTTCTTTTCTTGTGTGTTATTTGCTTGCATGATGTTGGTTGAGAGTCTTATGATGATAGTTGCTAGTATTGTGCCCAATTACCTCATGGGGATAATAGCTGGTGCTGGAATTCAAGGGCTTATGATGTTATCTGGTGGATTTTTCCAATTACCAAGTGATATTCCGGGACCGGTGTGGAAGTACCCAATGTACCACATTGCCTTCCACAAGTACGCATACCAGGGATTGTTCAAAAATGAATTCAAAGGAACAATGTTTCCGGCAAGTGAAGCCGGAGGGCCGTTCACCCTTAGCGGCGAGCAAATTTTGAGAGATATATGGCAAGTGGAAATGGGATATTCTAAGTGGGTGAATGTTGCTGTGTTGTTAGGGATGGTAGTTCTATATCGAATTCTGTTCTTGGTTATCATCAAGACTACAGAGAAGGTGAAGACTGTTAAAGCAGCTCTTCTGTCAACGTCTCCTAAGCAAAGCATGCAAGTATTGGCCAATCCCTCCTCTACCCCCGTGCATGGAGAGAACCACTTGCATAATGTCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005319 GO:0005342 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006970 GO:0007275 GO:0007389 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009611 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009897 GO:0009965 GO:0009986 GO:0010016 GO:0010033 GO:0010051 GO:0010154 GO:0010222 GO:0010588 GO:0010876 GO:0015075 GO:0015245 GO:0015318 GO:0015399 GO:0015405 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0022804 GO:0022857 GO:0031224 GO:0032501 GO:0032502 GO:0033036 GO:0033993 GO:0034220 GO:0042221 GO:0042623 GO:0042626 GO:0042802 GO:0042803 GO:0043492 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061458 GO:0071702 GO:0071944 GO:0080051 GO:0090698 GO:0097305 GO:0098552 GO:0098656 GO:0099402 GO:1901700 GO:1903825 GO:1905039 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

700

Amino Acids

77.06

Weight (kDa)

8.2

Isoelectric Point (pI)

39.13

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000287)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20390 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20400 FvH4_1g20410 FvH4_1g20410 FvH4_6g39250 FvH4_6g39250
malus_domestica	MD00G1036800.v1.1 MD05G1042600.v1.1 MD10G1049900.v1.1
prunus_persica	Prupe.6G191500_v2.0.a1 Prupe.8G064300_v2.0.a1 Prupe.8G064300_v2.0.a1 Prupe.8G064400_v2.0.a1
pyrus_communis	pycom05g03520 pycom10g03500 pycom10g03510 pycom15g28250
rosa_chinensis	RchiOBHm_Chr1g0336561 RchiOBHm_Chr2g0112551 RchiOBHm_Chr2g0112561 RchiOBHm_Chr2g0112631 RchiOBHm_Chr2g0112641 RchiOBHm_Chr2g0153091 RchiOBHm_Chr2g0153111 RchiOBHm_Chr2g0153141 RchiOBHm_Chr2g0153161 RchiOBHm_Chr2g0153171
rosa_laevigata	RLG00000017991 RLG00000017992 RLG00000017995 RLG00000017997 RLG00000020676 RLG00000020677 RLG00000020678 RLG00000020682
rosa_multiflora	Rmu_co8208234.1_g000001 Rmu_co8218328.1_g000001 Rmu_co8425243.1_g000001 Rmu_sc0000218.1_g000037 Rmu_sc0000218.1_g000044 Rmu_sc0000218.1_g000047 Rmu_sc0000218.1_g000052 Rmu_sc0001155.1_g000011 Rmu_sc0001155.1_g000026 Rmu_sc0001155.1_g000029 Rmu_sc0001499.1_g000001 Rmu_sc0001499.1_g000040 Rmu_sc0002401.1_g000032 Rmu_sc0002401.1_g000033 Rmu_sc0004070.1_g000003 Rmu_sc0004070.1_g000004 Rmu_sc0005547.1_g000001 Rmu_sc0006062.1_g000002 Rmu_sc0009780.1_g000005 Rmu_sc0013071.1_g000001 Rmu_sc0013071.1_g000012
rosa_roxburghii	Rroxscaffold_2G00095130 Rroxscaffold_2G00095140 Rroxscaffold_2G00095160 Rroxscaffold_2G00095180 Rroxscaffold_2G00095190 Rroxscaffold_2G00095200 Rroxscaffold_2G00131290 Rroxscaffold_2G00131320 Rroxscaffold_2G00131350
rosa_rugosa	Rorug02G0181500 Rorug02G0181500 Rorug02G0181600 Rorug02G0181700 Rorug02G0437300 Rorug02G0437400
rosa_samantha	Rh1DG154100 Rh2AG235200 Rh2AG235300 Rh2AG235400 Rh2AG235600 Rh2AG235800 Rh2AG499700 Rh2AG499800 Rh2AG500000 Rh2AG500100 Rh2AG500200 Rh2BG248500 Rh2BG248700 Rh2BG248800 Rh2BG511000 Rh2BG511200 Rh2BG511300 Rh2BG511600 Rh2CG239600 Rh2CG239700 Rh2CG239900 Rh2CG240000 Rh2CG485900 Rh2CG486000 Rh2CG486200 Rh2CG486300 Rh2CG486400 Rh2DG242500 Rh2DG242600 Rh2DG242700 Rh2DG242900 Rh2DG243100 Rh2DG522800 Rh2DG523100 Rh2DG523200 Rh2DG523500 Rh2DG523600 Rh5DG200200
rosa_wichuraiana	Rw2G018200 Rw2G018210 Rw2G018230 Rw2G018240 Rw2G041010 Rw2G041020 Rw2G041030 Rw2G041050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	134
AccB7I	CCANNNNNTGG	1 cut(s)	119
AciI	CCGC	3 cut(s)	123, 717, 1851
AclWI	GGATC	4 cut(s)	388, 966, 1047, 1296
AcoI	YGGCCR	3 cut(s)	640, 1359, 2043
AcsI	RAATTY	6 cut(s)	367, 1118, 1665, 1796, 1860, 1947
AcuI	CTGAAG	1 cut(s)	846
AcyI	GRCGYC	1 cut(s)	591
AfaI	GTAC	6 cut(s)	108, 1176, 1270, 1739, 1748, 1769
AfiI	CCNNNNNNNGG	4 cut(s)	119, 291, 716, 2069
AflII	CTTAAG	1 cut(s)	968
AflIII	ACRYGT	1 cut(s)	201
AgeI	ACCGGT	2 cut(s)	275, 1726
AjiI	CACGTC	1 cut(s)	202
AjnI	CCWGG	3 cut(s)	289, 1233, 1776
AleI	CACNNNNGTG	1 cut(s)	1431
AloI	GAACNNNNNNTCC	2 cut(s)	1798, 1830
Alw21I	GWGCWC	1 cut(s)	300
Alw26I	GTCTC	3 cut(s)	355, 1175, 2022
AlwI	GGATC	4 cut(s)	388, 966, 1047, 1296
Ama87I	CYCGRG	1 cut(s)	1502
AoxI	GGCC	8 cut(s)	313, 640, 683, 718, 1261, 1359, 1835, 2043
ApaI	GGGCCC	1 cut(s)	317
ApeKI	GCWGC	8 cut(s)	62, 246, 249, 511, 731, 899, 1324, 2000
ApoI	RAATTY	6 cut(s)	367, 1118, 1665, 1796, 1860, 1947
AseI	ATTAAT	1 cut(s)	15
AsiGI	ACCGGT	2 cut(s)	275, 1726
Asp700I	GAANNNNTTC	1 cut(s)	831
AspLEI	GCGC	1 cut(s)	1233
AspS9I	GGNCC	5 cut(s)	313, 314, 887, 1724, 1835
AsuC2I	CCSGG	3 cut(s)	1503, 1504, 1722
AsuHPI	GGTGA	6 cut(s)	220, 788, 1136, 1834, 1918, 1996
AsuII	TTCGAA	1 cut(s)	1545
AvaI	CYCGRG	1 cut(s)	1502
AvaII	GGWCC	2 cut(s)	887, 1724
BaeGI	GKGCMC	3 cut(s)	317, 1428, 1632
BalI	TGGCCA	3 cut(s)	642, 1361, 2045
BanI	GGYRCC	1 cut(s)	134
BanII	GRGCYC	2 cut(s)	300, 317
BbsI	GAAGAC	3 cut(s)	855, 1395, 1994
Bbv12I	GWGCWC	1 cut(s)	300
BbvI	GCAGC	8 cut(s)	74, 258, 261, 498, 743, 911, 1311, 2012
BccI	CCATC	6 cut(s)	571, 773, 815, 819, 961, 1924
BceAI	ACGGC	2 cut(s)	698, 1822
BcgI	CGANNNNNNTGC	4 cut(s)	1193, 1227, 1302, 1336
BciT130I	CCWGG	3 cut(s)	291, 1235, 1778
BcnI	CCSGG	3 cut(s)	1503, 1504, 1722
BcoDI	GTCTC	3 cut(s)	355, 1175, 2022
BfaI	CTAG	4 cut(s)	101, 819, 1259, 1620
BfmI	CTRYAG	2 cut(s)	897, 1974
BfrI	CTTAAG	1 cut(s)	968
BglI	GCCNNNNNGGC	2 cut(s)	321, 1152
BlpI	GCTNAGC	1 cut(s)	507
BmcAI	AGTACT	1 cut(s)	108
Bme1390I	CCNGG	6 cut(s)	291, 1235, 1503, 1504, 1722, 1778
Bme18I	GGWCC	2 cut(s)	887, 1724
BmeT110I	CYCGRG	1 cut(s)	1502
BmgBI	CACGTC	1 cut(s)	202
BmgT120I	GGNCC	5 cut(s)	313, 314, 887, 1724, 1835
BmiI	GGNNCC	3 cut(s)	136, 315, 1725
BmrFI	CCNGG	6 cut(s)	291, 1235, 1503, 1504, 1722, 1778
BmsI	GCATC	1 cut(s)	337
BpiI	GAAGAC	3 cut(s)	855, 1395, 1994
BpmI	CTGGAG	1 cut(s)	89
Bpu10I	CCTNAGC	2 cut(s)	1847, 2022
Bpu1102I	GCTNAGC	1 cut(s)	507
Bpu14I	TTCGAA	1 cut(s)	1545
BpuMI	CCSGG	3 cut(s)	1503, 1504, 1722
BsaBI	GATNNNNATC	1 cut(s)	398
BsaHI	GRCGYC	1 cut(s)	591
BsaJI	CCNNGG	6 cut(s)	290, 788, 1264, 1502, 1503, 1777
BsaWI	WCCGGW	3 cut(s)	275, 1538, 1726
BsaXI	ACNNNNNCTCC	2 cut(s)	188, 218
Bsc4I	CCNNNNNNNGG	4 cut(s)	119, 291, 716, 2069
Bse118I	RCCGGY	2 cut(s)	275, 1726
Bse1I	ACTGG	3 cut(s)	72, 426, 928
Bse3DI	GCAATG	1 cut(s)	1752
Bse8I	GATNNNNATC	1 cut(s)	398
BseBI	CCWGG	3 cut(s)	291, 1235, 1778
BseDI	CCNNGG	6 cut(s)	290, 788, 1264, 1502, 1503, 1777
BseGI	GGATG	3 cut(s)	803, 807, 1935
BseJI	GATNNNNATC	1 cut(s)	398
BseLI	CCNNNNNNNGG	4 cut(s)	119, 291, 716, 2069
BseMI	GCAATG	1 cut(s)	1752
BseMII	CTCAG	2 cut(s)	33, 521
BseNI	ACTGG	3 cut(s)	72, 426, 928
BseRI	GAGGAG	4 cut(s)	141, 144, 188, 2044
BseSI	GKGCMC	3 cut(s)	317, 1428, 1632
BseXI	GCAGC	8 cut(s)	74, 258, 261, 498, 743, 911, 1311, 2012
BsgI	GTGCAG	1 cut(s)	750
BshFI	GGCC	8 cut(s)	315, 642, 685, 720, 1263, 1361, 1837, 2045
BshNI	GGYRCC	1 cut(s)	134
BshTI	ACCGGT	2 cut(s)	275, 1726
BsiHKAI	GWGCWC	1 cut(s)	300
BsiHKCI	CYCGRG	1 cut(s)	1502
BsiSI	CCGG	8 cut(s)	138, 276, 1503, 1539, 1721, 1727, 1817, 1830
BslFI	GGGAC	1 cut(s)	1737
BslI	CCNNNNNNNGG	4 cut(s)	119, 291, 716, 2069
BsmAI	GTCTC	3 cut(s)	355, 1175, 2022
BsmBI	CGTCTC	1 cut(s)	2022
BsmFI	GGGAC	1 cut(s)	1737
BsnI	GGCC	8 cut(s)	315, 642, 685, 720, 1263, 1361, 1837, 2045
BsoBI	CYCGRG	1 cut(s)	1502
Bsp119I	TTCGAA	1 cut(s)	1545
Bsp120I	GGGCCC	1 cut(s)	313
Bsp1286I	GDGCHC	4 cut(s)	300, 317, 1428, 1632
Bsp143I	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
Bsp1720I	GCTNAGC	1 cut(s)	507
BspACI	CCGC	3 cut(s)	123, 717, 1851
BspANI	GGCC	8 cut(s)	315, 642, 685, 720, 1263, 1361, 1837, 2045
BspCNI	CTCAG	2 cut(s)	34, 520
BspHI	TCATGA	3 cut(s)	747, 1251, 1279
BspLI	GGNNCC	3 cut(s)	136, 315, 1725
BspMAI	CTGCAG	1 cut(s)	901
BspPI	GGATC	4 cut(s)	388, 966, 1047, 1296
BspQI	GCTCTTC	1 cut(s)	2010
BspT104I	TTCGAA	1 cut(s)	1545
BspT107I	GGYRCC	1 cut(s)	134
BspTI	CTTAAG	1 cut(s)	968
BsrDI	GCAATG	1 cut(s)	1752
BsrFI	RCCGGY	2 cut(s)	275, 1726
BsrI	ACTGG	3 cut(s)	72, 426, 928
BssAI	RCCGGY	2 cut(s)	275, 1726
BssECI	CCNNGG	6 cut(s)	290, 788, 1264, 1502, 1503, 1777
BssMI	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
BssNI	GRCGYC	1 cut(s)	591
BssT1I	CCWWGG	1 cut(s)	788
Bst2UI	CCWGG	3 cut(s)	291, 1235, 1778
Bst4CI	ACNGT	8 cut(s)	214, 236, 481, 728, 877, 1273, 1433, 1993
Bst6I	CTCTTC	4 cut(s)	345, 646, 1133, 2010
BstACI	GRCGYC	1 cut(s)	591
BstAFI	CTTAAG	1 cut(s)	968
BstAPI	GCANNNNNTGC	1 cut(s)	2032
BstBI	TTCGAA	1 cut(s)	1545
BstC8I	GCNNGC	6 cut(s)	121, 1153, 1322, 1582, 1856, 2033
BstDEI	CTNAG	5 cut(s)	42, 507, 1847, 1899, 2022
BstF5I	GGATG	3 cut(s)	803, 807, 1935
BstHHI	GCGC	1 cut(s)	1233
BstKTI	GATC	8 cut(s)	100, 257, 396, 961, 1055, 1190, 1213, 1304
BstMAI	GTCTC	3 cut(s)	355, 1175, 2022
BstMBI	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
BstMWI	GCNNNNNNNGC	7 cut(s)	295, 321, 517, 899, 1152, 1826, 2032
BstNI	CCWGG	3 cut(s)	291, 1235, 1778
BstNSI	RCATGY	2 cut(s)	1204, 2035
BstSCI	CCNGG	6 cut(s)	289, 1233, 1501, 1502, 1720, 1776
BstSFI	CTRYAG	2 cut(s)	897, 1974
BstSLI	GKGCMC	3 cut(s)	317, 1428, 1632
BstV1I	GCAGC	8 cut(s)	74, 258, 261, 498, 743, 911, 1311, 2012
BstV2I	GAAGAC	3 cut(s)	855, 1395, 1994
BstX2I	RGATCY	3 cut(s)	393, 1052, 1301
BstYI	RGATCY	3 cut(s)	393, 1052, 1301
BsuRI	GGCC	8 cut(s)	315, 642, 685, 720, 1263, 1361, 1837, 2045
BtrI	CACGTC	1 cut(s)	202
BtsCI	GGATG	3 cut(s)	803, 807, 1935
BtsIMutI	CAGTG	3 cut(s)	65, 419, 733
Cac8I	GCNNGC	6 cut(s)	121, 1153, 1322, 1582, 1856, 2033
CciI	TCATGA	3 cut(s)	747, 1251, 1279
CfoI	GCGC	1 cut(s)	1233
Cfr10I	RCCGGY	2 cut(s)	275, 1726
Cfr13I	GGNCC	5 cut(s)	313, 314, 887, 1724, 1835
Cfr9I	CCCGGG	1 cut(s)	1502
CseI	GACGC	1 cut(s)	599
Csp6I	GTAC	6 cut(s)	107, 1175, 1269, 1738, 1747, 1768
CspAI	ACCGGT	2 cut(s)	275, 1726
CspCI	CAANNNNNGTGG	2 cut(s)	325, 360
CviQI	GTAC	6 cut(s)	107, 1175, 1269, 1738, 1747, 1768
DdeI	CTNAG	5 cut(s)	42, 507, 1847, 1899, 2022
DpnI	GATC	8 cut(s)	99, 256, 395, 960, 1054, 1189, 1212, 1303
DpnII	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
EaeI	YGGCCR	3 cut(s)	640, 1359, 2043
Eam1104I	CTCTTC	4 cut(s)	345, 646, 1133, 2010
EarI	CTCTTC	4 cut(s)	345, 646, 1133, 2010
EciI	GGCGGA	1 cut(s)	138
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	788
Eco147I	AGGCCT	1 cut(s)	1263
Eco24I	GRGCYC	2 cut(s)	300, 317
Eco47I	GGWCC	2 cut(s)	887, 1724
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	1 cut(s)	846
Eco88I	CYCGRG	1 cut(s)	1502
EcoICRI	GAGCTC	1 cut(s)	298
EcoO109I	RGGNCCY	1 cut(s)	314
EcoRI	GAATTC	5 cut(s)	367, 1118, 1665, 1796, 1947
EcoRII	CCWGG	3 cut(s)	289, 1233, 1776
EcoT14I	CCWWGG	1 cut(s)	788
EcoT22I	ATGCAT	1 cut(s)	1206
EcoT38I	GRGCYC	2 cut(s)	300, 317
ErhI	CCWWGG	1 cut(s)	788
Esp3I	CGTCTC	1 cut(s)	2022
FalI	AAGNNNNNCTT	2 cut(s)	1989, 2021
FaqI	GGGAC	1 cut(s)	1737
FauNDI	CATATG	2 cut(s)	442, 2096
FokI	GGATG	3 cut(s)	790, 794, 1942
FriOI	GRGCYC	2 cut(s)	300, 317
FspBI	CTAG	4 cut(s)	101, 819, 1259, 1620
GlaI	GCGC	1 cut(s)	1232
GsuI	CTGGAG	1 cut(s)	89
HaeIII	GGCC	8 cut(s)	315, 642, 685, 720, 1263, 1361, 1837, 2045
HapII	CCGG	8 cut(s)	138, 276, 1503, 1539, 1721, 1727, 1817, 1830
HgaI	GACGC	1 cut(s)	599
HhaI	GCGC	1 cut(s)	1233
Hin1I	GRCGYC	1 cut(s)	591
Hin6I	GCGC	1 cut(s)	1231
HinP1I	GCGC	1 cut(s)	1231
HincII	GTYRAC	1 cut(s)	2013
HindII	GTYRAC	1 cut(s)	2013
HinfI	GANTC	8 cut(s)	141, 524, 654, 1064, 1255, 1371, 1542, 1600
HpaII	CCGG	8 cut(s)	138, 276, 1503, 1539, 1721, 1727, 1817, 1830
HphI	GGTGA	6 cut(s)	220, 788, 1136, 1834, 1918, 1996
Hpy166II	GTNNAC	7 cut(s)	28, 336, 1087, 1112, 1198, 1842, 2013
Hpy188I	TCNGA	4 cut(s)	541, 826, 958, 1069
Hpy188III	TCNNGA	8 cut(s)	344, 748, 767, 867, 1195, 1252, 1280, 1969
Hpy8I	GTNNAC	7 cut(s)	28, 336, 1087, 1112, 1198, 1842, 2013
Hpy99I	CGWCG	1 cut(s)	707
HpyAV	CCTTC	5 cut(s)	327, 1386, 1390, 1768, 1975
HpyCH4III	ACNGT	8 cut(s)	214, 236, 481, 728, 877, 1273, 1433, 1993
HpyCH4IV	ACGT	2 cut(s)	201, 2015
HpyF10VI	GCNNNNNNNGC	7 cut(s)	295, 321, 517, 899, 1152, 1826, 2032
HpyF3I	CTNAG	5 cut(s)	42, 507, 1847, 1899, 2022
HpySE526I	ACGT	2 cut(s)	201, 2015
Hsp92I	GRCGYC	1 cut(s)	591
HspAI	GCGC	1 cut(s)	1231
Kzo9I	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
LguI	GCTCTTC	1 cut(s)	2010
LmnI	GCTCC	1 cut(s)	295
Lsp1109I	GCAGC	8 cut(s)	74, 258, 261, 498, 743, 911, 1311, 2012
LweI	GCATC	1 cut(s)	337
MaeI	CTAG	4 cut(s)	101, 819, 1259, 1620
MaeII	ACGT	2 cut(s)	201, 2015
MaeIII	GTNAC	5 cut(s)	208, 445, 562, 799, 1012
MalI	GATC	8 cut(s)	99, 256, 395, 960, 1054, 1189, 1212, 1303
MboI	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
MfeI	CAATTG	1 cut(s)	1035
MflI	RGATCY	3 cut(s)	393, 1052, 1301
MhlI	GDGCHC	4 cut(s)	300, 317, 1428, 1632
MlsI	TGGCCA	3 cut(s)	642, 1361, 2045
MluNI	TGGCCA	3 cut(s)	642, 1361, 2045
MlyI	GAGTC	4 cut(s)	518, 663, 1249, 1609
Mox20I	TGGCCA	3 cut(s)	642, 1361, 2045
Mph1103I	ATGCAT	1 cut(s)	1206
MroXI	GAANNNNTTC	1 cut(s)	831
MscI	TGGCCA	3 cut(s)	642, 1361, 2045
MslI	CAYNNNNRTG	3 cut(s)	51, 1250, 1431
Msp20I	TGGCCA	3 cut(s)	642, 1361, 2045
MspA1I	CMGCKG	1 cut(s)	511
MspCI	CTTAAG	1 cut(s)	968
MspI	CCGG	8 cut(s)	138, 276, 1503, 1539, 1721, 1727, 1817, 1830
MspR9I	CCNGG	6 cut(s)	291, 1235, 1503, 1504, 1722, 1778
MunI	CAATTG	1 cut(s)	1035
MvaI	CCWGG	3 cut(s)	291, 1235, 1778
MwoI	GCNNNNNNNGC	7 cut(s)	295, 321, 517, 899, 1152, 1826, 2032
NciI	CCSGG	3 cut(s)	1503, 1504, 1722
NdeI	CATATG	2 cut(s)	442, 2096
NdeII	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
NlaIV	GGNNCC	3 cut(s)	136, 315, 1725
NmuCI	GTSAC	2 cut(s)	208, 445
NsiI	ATGCAT	1 cut(s)	1206
NspI	RCATGY	2 cut(s)	1204, 2035
NspV	TTCGAA	1 cut(s)	1545
OliI	CACNNNNGTG	1 cut(s)	1431
PaeI	GCATGC	1 cut(s)	2035
PagI	TCATGA	3 cut(s)	747, 1251, 1279
PceI	AGGCCT	1 cut(s)	1263
PciSI	GCTCTTC	1 cut(s)	2010
PdmI	GAANNNNTTC	1 cut(s)	831
PfeI	GAWTC	4 cut(s)	141, 1064, 1371, 1542
PflFI	GACNNNGTC	1 cut(s)	535
PflMI	CCANNNNNTGG	1 cut(s)	119
PfoI	TCCNGGA	1 cut(s)	1720
PinAI	ACCGGT	2 cut(s)	275, 1726
PleI	GAGTC	4 cut(s)	518, 662, 1249, 1608
PpsI	GAGTC	4 cut(s)	518, 662, 1249, 1608
PshBI	ATTAAT	1 cut(s)	15
Psp124BI	GAGCTC	1 cut(s)	300
Psp6I	CCWGG	3 cut(s)	289, 1233, 1776
PspGI	CCWGG	3 cut(s)	289, 1233, 1776
PspN4I	GGNNCC	3 cut(s)	136, 315, 1725
PspOMI	GGGCCC	1 cut(s)	313
PspPI	GGNCC	5 cut(s)	313, 314, 887, 1724, 1835
PstI	CTGCAG	1 cut(s)	901
PsuI	RGATCY	3 cut(s)	393, 1052, 1301
PsyI	GACNNNGTC	1 cut(s)	535
PvuII	CAGCTG	1 cut(s)	511
RsaI	GTAC	6 cut(s)	108, 1176, 1270, 1739, 1748, 1769
RsaNI	GTAC	6 cut(s)	107, 1175, 1269, 1738, 1747, 1768
RseI	CAYNNNNRTG	3 cut(s)	51, 1250, 1431
SacI	GAGCTC	1 cut(s)	300
SapI	GCTCTTC	1 cut(s)	2010
Sau3AI	GATC	8 cut(s)	97, 254, 393, 958, 1052, 1187, 1210, 1301
Sau96I	GGNCC	5 cut(s)	313, 314, 887, 1724, 1835
ScaI	AGTACT	1 cut(s)	108
SchI	GAGTC	4 cut(s)	518, 663, 1249, 1609
ScrFI	CCNGG	6 cut(s)	291, 1235, 1503, 1504, 1722, 1778
SduI	GDGCHC	4 cut(s)	300, 317, 1428, 1632
SfaNI	GCATC	1 cut(s)	337
SfcI	CTRYAG	2 cut(s)	897, 1974
SfuI	TTCGAA	1 cut(s)	1545
SinI	GGWCC	2 cut(s)	887, 1724
SmaI	CCCGGG	1 cut(s)	1504
SmiMI	CAYNNNNRTG	3 cut(s)	51, 1250, 1431
SmlI	CTYRAG	1 cut(s)	968
SmoI	CTYRAG	1 cut(s)	968
SphI	GCATGC	1 cut(s)	2035
SseBI	AGGCCT	1 cut(s)	1263
SsiI	CCGC	3 cut(s)	123, 717, 1851
SspI	AATATT	1 cut(s)	94
SspMI	CTAG	4 cut(s)	101, 819, 1259, 1620
SstI	GAGCTC	1 cut(s)	300
StuI	AGGCCT	1 cut(s)	1263
StyD4I	CCNGG	6 cut(s)	289, 1233, 1501, 1502, 1720, 1776
StyI	CCWWGG	1 cut(s)	788
TaaI	ACNGT	8 cut(s)	214, 236, 481, 728, 877, 1273, 1433, 1993
TaiI	ACGT	2 cut(s)	204, 2018
TaqI	TCGA	4 cut(s)	702, 1213, 1545, 1945
TatI	WGTACW	1 cut(s)	106
TauI	GCSGC	2 cut(s)	720, 1854
TfiI	GAWTC	4 cut(s)	141, 1064, 1371, 1542
TscAI	CASTG	3 cut(s)	72, 426, 733
TseFI	GTSAC	2 cut(s)	208, 445
TseI	GCWGC	8 cut(s)	62, 246, 249, 511, 731, 899, 1324, 2000
Tsp45I	GTSAC	2 cut(s)	208, 445
TspDTI	ATGAA	7 cut(s)	63, 611, 1240, 1324, 1345, 1409, 1809
TspMI	CCCGGG	1 cut(s)	1502
TspRI	CASTG	3 cut(s)	72, 426, 733
Tth111I	GACNNNGTC	1 cut(s)	535
Van91I	CCANNNNNTGG	1 cut(s)	119
Vha464I	CTTAAG	1 cut(s)	968
VpaK11BI	GGWCC	2 cut(s)	887, 1724
VspI	ATTAAT	1 cut(s)	15
XapI	RAATTY	6 cut(s)	367, 1118, 1665, 1796, 1860, 1947
XceI	RCATGY	2 cut(s)	1204, 2035
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1639
XmaI	CCCGGG	1 cut(s)	1502
XmnI	GAANNNNTTC	1 cut(s)	831
XspI	CTAG	4 cut(s)	101, 819, 1259, 1620
ZrmI	AGTACT	1 cut(s)	108
Zsp2I	ATGCAT	1 cut(s)	1206

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.