MD13G1114500.v1.1
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002839
Physical Location & Seq
Reverse (-)
8304762 .. 8307197
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1114500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGAGTATCCTTGGCATAACTTCCTACACGTTACATTTCGTTTCTTCTTTTCTTCTTTTTTGTGTTTTTCTTTTTTGTTCTCATAGTTTGTCCTTGTTATCTCCTGGCAGTGAAGGTGCAGGTGGGGGCCATGCTCCAGATATAATCAAAGTCCGTGGCGTGAAAAATGTATTACCGTCATCAACGAATCCCACGCGGCCTCTCACCTCAAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTAGCTTTTGCTGAATCAAGAATAAGGGCTGAAACAATTGCCGCAGAAGATATTTTGCACGATATGGGGGCAATTAGCATTGTATCTTCTGATTCACAGCTTATGGGTCGCATCGGAGAGGTGATAATCAGAACTTGGCAAACAGCTGACAAGATGAAGTCACAAAGAGGGTCGATAGAACCTAGTGGATCCAACAATGACAACTTCCGGATCAAACGATACATCGCAAACAAAATAGTTCTACAAGGACTCGAACCAGAGCCATGTACAAGAAGATTGAAGGTCTACACCACTAGCGCTGGCGTGGTGGGGAAGCTGGCTGATCTTGTTCTATGGAAGCCATCGTTCTTTGGCGCAAAACCAGAAATGGTGATCAAAGGTGGTGCAATTGCAAGGGCTAATATGGGTGATCCAAATGCAAGCATCCCCGCCCCTGAAACGGTTATTTCAAGGCCTATGTTTGGTGCATTTGGCAAGGCTGGTAGTGCTCATTCCATTGCTTTTGCAGCTGTAGATAACTGCGTTAAAGACTCGTATCGCCTTGACAAGAGAGTGGAAGCCGTGCGTAACGTTAGGAAACTCAGCAAACTCGACATGAAGCTCAATGACGCCCTACCAAATATAGAGGTTGATCCGGAAACATACAAGGTAACAGCAGACGGCGTGGTTCTTACCTGCAGTCCAGCCACCACAGTTCCACTATCTCGGAACTACTTCATGTTGGGCGTGATGGGCGTGAGTCAATCATTTAGTTTAGGAATGTAA

Protein Analysis

348

Amino Acids

37.56

Weight (kDa)

8.16

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 38 - 169 4.5e-18 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 110, 962
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 196
AccIII TCCGGA 2 cut(s) 486, 913
AciI CCGC 3 cut(s) 196, 321, 708
AclI AACGTT 1 cut(s) 849
AclWI GGATC 5 cut(s) 462, 475, 497, 683, 905
AcyI GRCGYC 1 cut(s) 888
AfaI GTAC 1 cut(s) 547
AfeI AGCGCT 1 cut(s) 577
AfiI CCNNNNNNNGG 3 cut(s) 262, 718, 740
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 2 cut(s) 559, 729
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 6 cut(s) 284, 379, 425, 595, 788, 880
AluI AGCT 6 cut(s) 284, 379, 425, 595, 788, 880
Alw21I GWGCWC 1 cut(s) 769
AlwI GGATC 5 cut(s) 462, 475, 497, 683, 905
Aor13HI TCCGGA 2 cut(s) 486, 913
Aor51HI AGCGCT 1 cut(s) 577
AoxI GGCC 3 cut(s) 127, 197, 731
ApeKI GCWGC 1 cut(s) 785
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 2 cut(s) 578, 635
AspS9I GGNCC 1 cut(s) 127
AsuHPI GGTGA 5 cut(s) 196, 245, 412, 661, 698
BamHI GGATCC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 1 cut(s) 797
BccI CCATC 4 cut(s) 235, 258, 628, 1003
BceAI ACGGC 2 cut(s) 824, 955
BciT130I CCWGG 1 cut(s) 105
BciVI GTATCC 1 cut(s) 17
BclI TGATCA 1 cut(s) 651
BfaI CTAG 2 cut(s) 462, 573
BfmI CTRYAG 3 cut(s) 216, 789, 955
BfoI RGCGCY 1 cut(s) 579
BfuAI ACCTGC 2 cut(s) 110, 962
BfuI GTATCC 1 cut(s) 17
BisI GCNGC 3 cut(s) 197, 321, 786
BlsI GCNGC 3 cut(s) 198, 322, 787
Bme1390I CCNGG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 128, 469
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 3 cut(s) 235, 399, 711
BpmI CTGGAG 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 888
BsaJI CCNNGG 2 cut(s) 10, 154
BsaWI WCCGGW 2 cut(s) 486, 913
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 718, 740
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 225
BseAI TCCGGA 2 cut(s) 486, 913
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 10, 154
BseGI GGATG 1 cut(s) 702
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 3 cut(s) 262, 718, 740
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 1 cut(s) 874
BseXI GCAGC 1 cut(s) 797
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 3 cut(s) 129, 199, 733
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 2 cut(s) 487, 914
BslI CCNNNNNNNGG 3 cut(s) 262, 718, 740
BsnI GGCC 3 cut(s) 129, 199, 733
Bsp1286I GDGCHC 1 cut(s) 769
Bsp13I TCCGGA 2 cut(s) 486, 913
Bsp1407I TGTACA 1 cut(s) 545
Bsp143I GATC 6 cut(s) 467, 489, 601, 651, 688, 910
BspACI CCGC 3 cut(s) 196, 321, 708
BspANI GGCC 3 cut(s) 129, 199, 733
BspCNI CTCAG 1 cut(s) 873
BspEI TCCGGA 2 cut(s) 486, 913
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 128, 469
BspMAI CTGCAG 1 cut(s) 959
BspMI ACCTGC 2 cut(s) 110, 962
BspPI GGATC 5 cut(s) 462, 475, 497, 683, 905
BsrDI GCAATG 1 cut(s) 774
BsrGI TGTACA 1 cut(s) 545
BssECI CCNNGG 2 cut(s) 10, 154
BssMI GATC 6 cut(s) 467, 489, 601, 651, 688, 910
BssNI GRCGYC 1 cut(s) 888
BssT1I CCWWGG 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 3 cut(s) 177, 721, 973
BstACI GRCGYC 1 cut(s) 888
BstAUI TGTACA 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 580, 597, 700
BstDEI CTNAG 1 cut(s) 860
BstDSI CCRYGG 1 cut(s) 154
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 1 cut(s) 702
BstFNI CGCG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 2 cut(s) 578, 635
BstKTI GATC 6 cut(s) 470, 492, 604, 654, 691, 913
BstMBI GATC 6 cut(s) 467, 489, 601, 651, 688, 910
BstMWI GCNNNNNNNGC 3 cut(s) 764, 785, 1011
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 3 cut(s) 216, 789, 955
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 797
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 3 cut(s) 129, 199, 733
BtgI CCRYGG 1 cut(s) 154
BtgZI GCGATG 1 cut(s) 487
BtsCI GGATG 1 cut(s) 702
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 115
BveI ACCTGC 2 cut(s) 110, 962
Cac8I GCNNGC 3 cut(s) 580, 597, 700
CfoI GCGC 2 cut(s) 578, 635
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 896
Csp6I GTAC 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 4 cut(s) 131, 543, 874, 997
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 1 cut(s) 860
DpnI GATC 6 cut(s) 469, 491, 603, 653, 690, 912
DpnII GATC 6 cut(s) 467, 489, 601, 651, 688, 910
Eco130I CCWWGG 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 733
Eco47III AGCGCT 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 4 cut(s) 134, 546, 877, 1000
FatI CATG 4 cut(s) 130, 542, 873, 996
FauI CCCGC 1 cut(s) 715
FbaI TGATCA 1 cut(s) 651
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 3 cut(s) 197, 321, 786
FokI GGATG 1 cut(s) 689
Fsp4HI GCNGC 3 cut(s) 197, 321, 786
FspBI CTAG 2 cut(s) 462, 573
GlaI GCGC 2 cut(s) 577, 634
GluI GCNGC 3 cut(s) 197, 321, 786
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 3 cut(s) 129, 199, 733
HapII CCGG 2 cut(s) 487, 914
HgaI GACGC 1 cut(s) 896
HhaI GCGC 2 cut(s) 578, 635
Hin1I GRCGYC 1 cut(s) 888
Hin1II CATG 4 cut(s) 134, 546, 877, 1000
Hin6I GCGC 2 cut(s) 576, 633
HinP1I GCGC 2 cut(s) 576, 633
HinfI GANTC 6 cut(s) 187, 293, 371, 528, 809, 1018
HpaII CCGG 2 cut(s) 487, 914
HphI GGTGA 5 cut(s) 196, 245, 412, 661, 698
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 4 cut(s) 370, 395, 410, 987
Hpy188III TCNNGA 6 cut(s) 137, 230, 272, 297, 487, 914
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 2 cut(s) 107, 553
HpyCH4III ACNGT 3 cut(s) 177, 721, 973
HpyCH4IV ACGT 2 cut(s) 29, 849
HpyCH4V TGCA 8 cut(s) 119, 337, 665, 671, 698, 746, 785, 957
HpyF10VI GCNNNNNNNGC 3 cut(s) 764, 785, 1011
HpyF3I CTNAG 1 cut(s) 860
HpySE526I ACGT 2 cut(s) 29, 849
Hsp92I GRCGYC 1 cut(s) 888
Hsp92II CATG 4 cut(s) 134, 546, 877, 1000
HspAI GCGC 2 cut(s) 576, 633
Kpn2I TCCGGA 2 cut(s) 486, 913
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 6 cut(s) 467, 489, 601, 651, 688, 910
LmnI GCTCC 1 cut(s) 139
Lsp1109I GCAGC 1 cut(s) 797
LweI GCATC 3 cut(s) 235, 399, 711
MaeI CTAG 2 cut(s) 462, 573
MaeII ACGT 2 cut(s) 29, 849
MaeIII GTNAC 4 cut(s) 30, 438, 845, 928
MalI GATC 6 cut(s) 469, 491, 603, 653, 690, 912
MboI GATC 6 cut(s) 467, 489, 601, 651, 688, 910
MboII GAAGA 6 cut(s) 36, 44, 287, 338, 357, 564
MfeI CAATTG 2 cut(s) 315, 666
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 769
MluCI AATT 3 cut(s) 315, 351, 666
MlyI GAGTC 3 cut(s) 522, 803, 1027
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 5 cut(s) 210, 217, 391, 440, 898
MroI TCCGGA 2 cut(s) 486, 913
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 425, 788
MspI CCGG 2 cut(s) 487, 914
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 2 cut(s) 315, 666
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 764, 785, 1011
NdeII GATC 6 cut(s) 467, 489, 601, 651, 688, 910
NlaIII CATG 4 cut(s) 134, 546, 877, 1000
NlaIV GGNNCC 2 cut(s) 128, 469
NmuCI GTSAC 1 cut(s) 438
PaqCI CACCTGC 1 cut(s) 110
PceI AGGCCT 1 cut(s) 733
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 187, 293, 371
PkrI GCNGC 3 cut(s) 198, 322, 787
PleI GAGTC 3 cut(s) 522, 803, 1026
PpsI GAGTC 3 cut(s) 522, 803, 1026
Psp1406I AACGTT 1 cut(s) 849
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 128, 469
PspPI GGNCC 1 cut(s) 127
PstI CTGCAG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 467
PvuII CAGCTG 2 cut(s) 425, 788
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SaqAI TTAA 1 cut(s) 804
SatI GCNGC 3 cut(s) 197, 321, 786
Sau3AI GATC 6 cut(s) 467, 489, 601, 651, 688, 910
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 3 cut(s) 522, 803, 1027
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 3 cut(s) 235, 399, 711
SfcI CTRYAG 3 cut(s) 216, 789, 955
Sse9I AATT 3 cut(s) 315, 351, 666
SseBI AGGCCT 1 cut(s) 733
SsiI CCGC 3 cut(s) 196, 321, 708
SspMI CTAG 2 cut(s) 462, 573
StuI AGGCCT 1 cut(s) 733
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 3 cut(s) 177, 721, 973
TaiI ACGT 2 cut(s) 32, 852
TaqI TCGA 3 cut(s) 452, 531, 870
TasI AATT 3 cut(s) 315, 351, 666
TatI WGTACW 1 cut(s) 545
TauI GCSGC 2 cut(s) 199, 323
TfiI GAWTC 3 cut(s) 187, 293, 371
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 438
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 1 cut(s) 438
TspDTI ATGAA 3 cut(s) 449, 890, 985
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 115
XagI CCTNNNNNAGG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 2 cut(s) 462, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.