Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0002839

Physical Location & Seq

Genomic Coordinates Forward (+)

26426151 .. 26432567

Gene Structure 6417 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g24160.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2517 bp

ATGAAGCTGGTTCCAAGAGAGCTTGACAAATTGGGTCTCCACAATGCTGGGTTTCTAGCCCAGAAGAGACTTGCTCGTGGGTTAAGGCTCAACCACCCTGAAGCTGTTGCACTCATAGCCACTCAGATATTGGAGTTTGTTAGAGATGGTGATAAGTCTGTGGCAGAGTTGATGGACATTGGGAAACAGCTTCTGGGGAGGAAGCAAGTTCTTCCTGGTGTACAATATCTTTTGGATATGGTGCAGGTTGAAGGGACGTTTCGTGATGGGACCAAGTTAGTCACCATTCATGATCCAATTTCTTGTGAAAATGGAGATCTAGAACTAGCTTTAAAGAACTCTTTTCTTCCTGTTCCCTCAGTTGATAAGTTTCCAGAAATGGCGGATGGTAGAATTCCTGGCCAAATGATCTTTGAAGGTGGTAATATTGTAATTAATGAGGGAAGGAAGGCAATAATTCTGAAAATCGTCAACACTGGAGATAGGCCAGTTCAGGTCGGCAGTCACTATCACTTTATTGAGGTCAACCCCCACTTGGTCTTTGATCGAATGAAAGCACATGGTATGCGCCTGAATATACCTGCTGGAACAGCCAAGAGATTTGAGCCGGGGGAAAGTAAACATGTTGTACTTGTAAGCATTGGAGGTAATAGAGTAATCAGAGGAGGGAATGGCATTGTTGATGGTGCAGTACGAAGCTTTGACAGACACCATGTAGTCAAGGGAGCTTTGGAAGCCCGACAATTTGGGAACGAAGAAGAACCAAATGCTAGCGAAGGTGTTGCAGGAAGAGATGCAGCTTTTACCACTGTAATGTCTCGCGAGTCATATGCAAACATGTACGGCCCTACTACTGGTGACAAAATTCGGCTTGGTGACACCAACTTGTTTGCTGAAATTGAAAAGGACTATACTGTTTATGGGGATGAATCTGTATTTGGAGGTGGCAAAGTGATAAGAGATGGAATGGGCCAGTCAACAGGGAATCGAGCAGCTGACTCATTGGATACAGTTATAACTAATGCTGTAATTATTGACTATAGTGGGATCTTTAAGGCAGATATCGGTATCAAAGATGGTCATATAGTTGCCCTTGGGAAATCAGGCAATCCAGATGTTATGCATGGTTTATCTAGCAGTATGATCATTGGGGTTAACACAGAGGTCATTGCGGCAGAAGGACAAATTGTGACTGCAGGAGCTATAGATTGTCATGTGCATTTCATATGCCCGCAGTTGGCATATGAAGCCATATCAAGTGGTATTACAACCGTAGTGGGAGGTGGAACAGGACCTGCAACAGGGACTCGTGCAACAACATGCACACCATCTCCATTGCAAATGAAGTTCATGCTGCAATCAACTGATGAAGTGCCTCTAAATTTTGGCTTCACAGGGAAGGGGAACAGTGCCAAACCTGATGGGCTACATGAGATAATCGAGGCTGGAGCAATGGGTCTGAAGCTACATGAAGACTGGGGCACAACTCCTGCTGCAATAGACAATTGTTTAAGTGTTGGAGACCAATATGACATCCAGGTTAATATCCACACAGACACCTTGAATGAATCTGGATTTGTGGAACACACAATTGCGGCATTTAAAGACAGAACTATCCATACTTACCACAGTGAAGGTGCAGGTGGGGGTCATGCTCCAGATATCATCAAAGTATGTGGTGTGGAAAATGTCCTGCCCTCATCAACGAACCCCACACGACCTTTCACCTGCAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTAGCTTTTGCTGAATCAAGGATAAGAGCTGAAACAATTGCTGCAGAGGATATATTGCATGATATGGGGGCAATTAGCATGGTGGCTTCTGATTCACAGGCCATGGGTCGCATTGGAGAGGTGATAATCAGAACTTGGCAAACTGCTGACAAGATGAAGTCACAAAGAGGTTCGATAGAACCCAGTGGATCCAACAATGACAACCTCCGGATCAAGCGTTACATTGCAAAGTACACCATAAATCCAGCAATAGCAAATGGATTTTCTGAATATGTTGGTTCCGTTCAGGTGGGCAAGTTTGCTGATCTTGTTCTATGGAAGCCATCTTTCTTTGGGGCAAAACCAGAAATGGTGGTTAAAGGTGGTGCAATTGCATGGGCTAATATGGGTGATCCAAATGCAAGCATCCCTACACCTGAACCGGTCCTCTCGAGACCAATGTTTGGTGCATTTGGCAAGGCCAGTAGTGCTCAGTCCATTGCCTTTGTCAGCAAGGCAGCTAAAGAAAATGGGGTTAAAGTCTTGTATGACCTCAACAAGAGAGTAGAAGCTGTGGGAAATGTTAGGAATCTGCGCAAACGCGACATGCAGCTCAATGACGCCCTACCAAAGATTCTGGTTGACCCTGAGACATACACGGTGACAGCAGACGGCGAGGTTCTGACCTGCAGTGCAGCCACCACCGTTCCCTTATCCAGGAATTACTTCCTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006807 GO:0008150 GO:0008152 GO:0009039 GO:0016787 GO:0016810 GO:0016811

KEGG Pathways

Metabolic & Signaling

ko00220 ko00230 ko00791 ko01100 ko01120 map00220 map00230 map00791 map01100 map01120

Pfam Domains

Protein Families

Amidohydro_1 Urease_alpha Urease_beta Urease_gamma

Protein Analysis

839

Amino Acids

90.21

Weight (kDa)

5.8

Isoelectric Point (pI)

29.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Urease_gamma	PF00547	1 - 100	3.9e-42	Urease, gamma subunit
Urease_beta	PF00699	133 - 229	2.1e-41	Urease beta subunit
Urease_alpha	PF00449	272 - 390	4.2e-56	Urease alpha-subunit, N-terminal domain
Amidohydro_1	PF01979	396 - 723	5.4e-76	Amidohydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002839)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67550
fragaria_vesca	FvH4_4g24160 FvH4_4g24160 FvH4_4g24160
malus_domestica	MD13G1114100.v1.1 MD13G1114500.v1.1 MD13G1114900.v1.1 MD13G1115100.v1.1 MD16G1113300.v1.1 MD16G1113700.v1.1
prunus_persica	Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1
pyrus_communis	pycom13g09950 pycom16g09630 pycom16g09640 pycom16g09650
rosa_chinensis	RchiOBHm_Chr1g0326041 RchiOBHm_Chr4g0430931
rosa_laevigata	RLG00000006937
rosa_multiflora	Rmu_sc0006022.1_g000001 Rmu_ssc0000176.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223360 Rroxscaffold_5G00372620
rosa_rugosa	Rorug02G0437300 Rorug04G0247300 Rorug05G0134300
rosa_samantha	Rh2BG408000 Rh4AG303000 Rh4AG303900 Rh4BG311200 Rh4CG327500 Rh4DG307300 Rh7CG419200 Rh7CG419300 Rh7CG439900
rosa_wichuraiana	Rw4G026340 Rw7G035140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1016
AarI	CACCTGC	2 cut(s)	1631, 1736
Acc16I	TGCGCA	1 cut(s)	2375
Acc36I	ACCTGC	6 cut(s)	235, 589, 1303, 1631, 1736, 2474
AccB7I	CCANNNNNTGG	1 cut(s)	2243
AccII	CGCG	2 cut(s)	822, 2382
AccIII	TCCGGA	1 cut(s)	2007
AciI	CCGC	4 cut(s)	383, 1172, 1232, 1595
AclWI	GGATC	6 cut(s)	287, 1055, 1983, 1996, 2018, 2186
AcoI	YGGCCR	1 cut(s)	400
AcsI	RAATTY	3 cut(s)	393, 864, 1381
AcuI	CTGAAG	2 cut(s)	120, 1481
AcyI	GRCGYC	1 cut(s)	2400
AfaI	GTAC	5 cut(s)	222, 630, 693, 842, 2033
AfiI	CCNNNNNNNGG	7 cut(s)	535, 854, 1237, 1301, 1783, 2243, 2496
AflIII	ACRYGT	2 cut(s)	622, 837
AgeI	ACCGGT	1 cut(s)	2221
AgsI	TTSAA	4 cut(s)	251, 416, 902, 1564
AjnI	CCWGG	4 cut(s)	214, 397, 1536, 2495
AjuI	GAANNNNNNNTTGG	2 cut(s)	921, 953
Alw21I	GWGCWC	1 cut(s)	2272
Alw26I	GTCTC	6 cut(s)	41, 61, 822, 1515, 2227, 2423
AlwI	GGATC	6 cut(s)	287, 1055, 1983, 1996, 2018, 2186
AlwNI	CAGNNNCTG	2 cut(s)	193, 1295
Ama87I	CYCGRG	1 cut(s)	2230
Aor13HI	TCCGGA	1 cut(s)	2007
AoxI	GGCC	6 cut(s)	400, 485, 844, 970, 1899, 2259
ApeKI	GCWGC	8 cut(s)	797, 992, 1354, 1493, 1841, 2297, 2389, 2474
ApoI	RAATTY	3 cut(s)	393, 864, 1381
AseI	ATTAAT	1 cut(s)	435
AsiGI	ACCGGT	1 cut(s)	2221
Asp700I	GAANNNNTTC	1 cut(s)	2504
AspLEI	GCGC	2 cut(s)	570, 2376
AspS9I	GGNCC	5 cut(s)	270, 845, 970, 1292, 2224
AsuC2I	CCSGG	1 cut(s)	609
AsuHPI	GGTGA	9 cut(s)	161, 274, 869, 887, 1717, 1766, 1933, 2201, 2452
AsuNHI	GCTAGC	1 cut(s)	770
AvaI	CYCGRG	1 cut(s)	2230
AvaII	GGWCC	3 cut(s)	270, 1292, 2224
BaeGI	GKGCMC	1 cut(s)	1484
BalI	TGGCCA	1 cut(s)	402
BamHI	GGATCC	1 cut(s)	1988
BauI	CACGAG	2 cut(s)	75, 1308
BbsI	GAAGAC	1 cut(s)	1479
Bbv12I	GWGCWC	1 cut(s)	2272
BbvI	GCAGC	8 cut(s)	809, 1004, 1341, 1480, 1828, 2309, 2401, 2486
BceAI	ACGGC	2 cut(s)	859, 2467
BcgI	CGANNNNNNTGC	4 cut(s)	764, 798, 812, 846
BciT130I	CCWGG	4 cut(s)	216, 399, 1538, 2497
BciVI	GTATCC	1 cut(s)	1000
BclI	TGATCA	1 cut(s)	1143
BcnI	CCSGG	1 cut(s)	609
BcoDI	GTCTC	6 cut(s)	41, 61, 822, 1515, 2227, 2423
BfaI	CTAG	5 cut(s)	56, 320, 326, 771, 1134
BfmI	CTRYAG	6 cut(s)	1039, 1194, 1203, 1737, 1842, 2467
BfuAI	ACCTGC	6 cut(s)	235, 589, 1303, 1631, 1736, 2474
BfuI	GTATCC	1 cut(s)	1000
BglII	AGATCT	1 cut(s)	316
Bme1390I	CCNGG	5 cut(s)	216, 399, 609, 1538, 2497
Bme18I	GGWCC	3 cut(s)	270, 1292, 2224
BmeT110I	CYCGRG	1 cut(s)	2230
BmgT120I	GGNCC	5 cut(s)	270, 845, 970, 1292, 2224
BmiI	GGNNCC	4 cut(s)	12, 271, 1990, 2080
BmrFI	CCNGG	5 cut(s)	216, 399, 609, 1538, 2497
BmrI	ACTGGG	2 cut(s)	1486, 1977
BmsI	GCATC	3 cut(s)	784, 1756, 2214
BmtI	GCTAGC	1 cut(s)	774
BmuI	ACTGGG	2 cut(s)	1486, 1977
BpiI	GAAGAC	1 cut(s)	1479
BplI	GAGNNNNNCTC	2 cut(s)	58, 90
BpmI	CTGGAG	3 cut(s)	498, 1467, 1641
BpuMI	CCSGG	1 cut(s)	609
BsaBI	GATNNNNATC	1 cut(s)	1746
BsaHI	GRCGYC	1 cut(s)	2400
BsaI	GGTCTC	3 cut(s)	41, 1515, 2227
BsaJI	CCNNGG	3 cut(s)	608, 1093, 1902
BsaWI	WCCGGW	2 cut(s)	2007, 2221
BsaXI	ACNNNNNCTCC	2 cut(s)	1315, 1345
Bsc4I	CCNNNNNNNGG	7 cut(s)	535, 854, 1237, 1301, 1783, 2243, 2496
Bse118I	RCCGGY	1 cut(s)	2221
Bse1I	ACTGG	7 cut(s)	481, 488, 859, 973, 1481, 1983, 2262
Bse3DI	GCAATG	5 cut(s)	1167, 1334, 1458, 2022, 2277
Bse8I	GATNNNNATC	1 cut(s)	1746
BseAI	TCCGGA	1 cut(s)	2007
BseBI	CCWGG	4 cut(s)	216, 399, 1538, 2497
BseDI	CCNNGG	3 cut(s)	608, 1093, 1902
BseGI	GGATG	4 cut(s)	391, 931, 1533, 2205
BseJI	GATNNNNATC	1 cut(s)	1746
BseLI	CCNNNNNNNGG	7 cut(s)	535, 854, 1237, 1301, 1783, 2243, 2496
BseMI	GCAATG	5 cut(s)	1167, 1334, 1458, 2022, 2277
BseMII	CTCAG	4 cut(s)	137, 372, 2285, 2418
BseNI	ACTGG	7 cut(s)	481, 488, 859, 973, 1481, 1983, 2262
BseRI	GAGGAG	1 cut(s)	678
BseSI	GKGCMC	1 cut(s)	1484
BseXI	GCAGC	8 cut(s)	809, 1004, 1341, 1480, 1828, 2309, 2401, 2486
BseYI	CCCAGC	1 cut(s)	47
BsgI	GTGCAG	4 cut(s)	263, 708, 1659, 2493
Bsh1236I	CGCG	2 cut(s)	822, 2382
BshFI	GGCC	6 cut(s)	402, 487, 846, 972, 1901, 2261
BshTI	ACCGGT	1 cut(s)	2221
BsiHKAI	GWGCWC	1 cut(s)	2272
BsiHKCI	CYCGRG	1 cut(s)	2230
BsiSI	CCGG	3 cut(s)	608, 2008, 2222
BslFI	GGGAC	3 cut(s)	268, 283, 1318
BslI	CCNNNNNNNGG	7 cut(s)	535, 854, 1237, 1301, 1783, 2243, 2496
BsmAI	GTCTC	6 cut(s)	41, 61, 822, 1515, 2227, 2423
BsmFI	GGGAC	3 cut(s)	268, 283, 1318
BsnI	GGCC	6 cut(s)	402, 487, 846, 972, 1901, 2261
Bso31I	GGTCTC	3 cut(s)	41, 1515, 2227
BsoBI	CYCGRG	1 cut(s)	2230
Bsp1286I	GDGCHC	2 cut(s)	1484, 2272
Bsp13I	TCCGGA	1 cut(s)	2007
Bsp1407I	TGTACA	1 cut(s)	220
Bsp19I	CCATGG	1 cut(s)	1902
Bsp68I	TCGCGA	1 cut(s)	822
BspACI	CCGC	4 cut(s)	383, 1172, 1232, 1595
BspANI	GGCC	6 cut(s)	402, 487, 846, 972, 1901, 2261
BspCNI	CTCAG	4 cut(s)	136, 371, 2284, 2419
BspEI	TCCGGA	1 cut(s)	2007
BspFNI	CGCG	2 cut(s)	822, 2382
BspHI	TCATGA	1 cut(s)	289
BspLI	GGNNCC	4 cut(s)	12, 271, 1990, 2080
BspMAI	CTGCAG	3 cut(s)	1198, 1846, 2471
BspMI	ACCTGC	6 cut(s)	235, 589, 1303, 1631, 1736, 2474
BspOI	GCTAGC	1 cut(s)	774
BspPI	GGATC	6 cut(s)	287, 1055, 1983, 1996, 2018, 2186
BspTNI	GGTCTC	3 cut(s)	41, 1515, 2227
BsrDI	GCAATG	5 cut(s)	1167, 1334, 1458, 2022, 2277
BsrFI	RCCGGY	1 cut(s)	2221
BsrGI	TGTACA	1 cut(s)	220
BsrI	ACTGG	7 cut(s)	481, 488, 859, 973, 1481, 1983, 2262
BssAI	RCCGGY	1 cut(s)	2221
BssECI	CCNNGG	3 cut(s)	608, 1093, 1902
BssNI	GRCGYC	1 cut(s)	2400
BssSI	CACGAG	2 cut(s)	75, 1308
BssT1I	CCWWGG	2 cut(s)	1093, 1902
Bst2BI	CACGAG	2 cut(s)	75, 1308
Bst2UI	CCWGG	4 cut(s)	216, 399, 1538, 2497
Bst4CI	ACNGT	8 cut(s)	811, 916, 1012, 1273, 1409, 1631, 2440, 2485
Bst6I	CTCTTC	2 cut(s)	59, 784
BstACI	GRCGYC	1 cut(s)	2400
BstAUI	TGTACA	1 cut(s)	220
BstC8I	GCNNGC	3 cut(s)	772, 1232, 2203
BstDEI	CTNAG	4 cut(s)	123, 358, 2271, 2427
BstDSI	CCRYGG	1 cut(s)	1902
BstENI	CCTNNNNNAGG	3 cut(s)	1299, 1781, 2494
BstF5I	GGATG	4 cut(s)	391, 931, 1533, 2205
BstFNI	CGCG	2 cut(s)	822, 2382
BstHHI	GCGC	2 cut(s)	570, 2376
BstMAI	GTCTC	6 cut(s)	41, 61, 822, 1515, 2227, 2423
BstMWI	GCNNNNNNNGC	5 cut(s)	116, 590, 734, 1247, 2267
BstNI	CCWGG	4 cut(s)	216, 399, 1538, 2497
BstNSI	RCATGY	4 cut(s)	626, 841, 1323, 2389
BstSCI	CCNGG	5 cut(s)	214, 397, 607, 1536, 2495
BstSFI	CTRYAG	6 cut(s)	1039, 1194, 1203, 1737, 1842, 2467
BstSLI	GKGCMC	1 cut(s)	1484
BstUI	CGCG	2 cut(s)	822, 2382
BstV1I	GCAGC	8 cut(s)	809, 1004, 1341, 1480, 1828, 2309, 2401, 2486
BstV2I	GAAGAC	1 cut(s)	1479
BstX2I	RGATCY	3 cut(s)	316, 1047, 1988
BstXI	CCANNNNNNTGG	1 cut(s)	47
BstYI	RGATCY	3 cut(s)	316, 1047, 1988
BsuI	GTATCC	1 cut(s)	1000
BsuRI	GGCC	6 cut(s)	402, 487, 846, 972, 1901, 2261
BtgI	CCRYGG	1 cut(s)	1902
BtsCI	GGATG	4 cut(s)	391, 931, 1533, 2205
BtsI	GCAGTG	1 cut(s)	2476
BtsIMutI	CAGTG	6 cut(s)	474, 807, 1414, 1636, 1990, 2476
BtuMI	TCGCGA	1 cut(s)	822
BveI	ACCTGC	6 cut(s)	235, 589, 1303, 1631, 1736, 2474
Cac8I	GCNNGC	3 cut(s)	772, 1232, 2203
CaiI	CAGNNNCTG	2 cut(s)	193, 1295
CciI	TCATGA	1 cut(s)	289
CfoI	GCGC	2 cut(s)	570, 2376
Cfr10I	RCCGGY	1 cut(s)	2221
Cfr13I	GGNCC	5 cut(s)	270, 845, 970, 1292, 2224
CseI	GACGC	1 cut(s)	2408
Csp6I	GTAC	5 cut(s)	221, 629, 692, 841, 2032
CspAI	ACCGGT	1 cut(s)	2221
CspCI	CAANNNNNGTGG	4 cut(s)	1257, 1292, 1657, 1692
CviQI	GTAC	5 cut(s)	221, 629, 692, 841, 2032
DdeI	CTNAG	4 cut(s)	123, 358, 2271, 2427
DraI	TTTAAA	2 cut(s)	333, 1603
EaeI	YGGCCR	1 cut(s)	400
Eam1104I	CTCTTC	2 cut(s)	59, 784
EarI	CTCTTC	2 cut(s)	59, 784
EciI	GGCGGA	1 cut(s)	398
Eco130I	CCWWGG	2 cut(s)	1093, 1902
Eco31I	GGTCTC	3 cut(s)	41, 1515, 2227
Eco32I	GATATC	2 cut(s)	1063, 1663
Eco47I	GGWCC	3 cut(s)	270, 1292, 2224
Eco57I	CTGAAG	2 cut(s)	120, 1481
Eco88I	CYCGRG	1 cut(s)	2230
EcoNI	CCTNNNNNAGG	3 cut(s)	1299, 1781, 2494
EcoO109I	RGGNCCY	1 cut(s)	1292
EcoRI	GAATTC	1 cut(s)	393
EcoRII	CCWGG	4 cut(s)	214, 397, 1536, 2495
EcoRV	GATATC	2 cut(s)	1063, 1663
EcoT14I	CCWWGG	2 cut(s)	1093, 1902
EcoT22I	ATGCAT	1 cut(s)	1125
ErhI	CCWWGG	2 cut(s)	1093, 1902
FaqI	GGGAC	3 cut(s)	268, 283, 1318
FauI	CCCGC	1 cut(s)	1239
FauNDI	CATATG	3 cut(s)	829, 1226, 1243
FbaI	TGATCA	1 cut(s)	1143
FokI	GGATG	4 cut(s)	398, 938, 1520, 2192
FspBI	CTAG	5 cut(s)	56, 320, 326, 771, 1134
FspI	TGCGCA	1 cut(s)	2375
GlaI	GCGC	2 cut(s)	569, 2375
GsaI	CCCAGC	1 cut(s)	51
GsuI	CTGGAG	3 cut(s)	498, 1467, 1641
HaeIII	GGCC	6 cut(s)	402, 487, 846, 972, 1901, 2261
HapII	CCGG	3 cut(s)	608, 2008, 2222
HgaI	GACGC	1 cut(s)	2408
HhaI	GCGC	2 cut(s)	570, 2376
Hin1I	GRCGYC	1 cut(s)	2400
Hin6I	GCGC	2 cut(s)	568, 2374
HinP1I	GCGC	2 cut(s)	568, 2374
HincII	GTYRAC	5 cut(s)	472, 526, 978, 1156, 2422
HindII	GTYRAC	5 cut(s)	472, 526, 978, 1156, 2422
HindIII	AAGCTT	1 cut(s)	697
HpaI	GTTAAC	1 cut(s)	1156
HpaII	CCGG	3 cut(s)	608, 2008, 2222
HphI	GGTGA	9 cut(s)	161, 274, 869, 887, 1717, 1766, 1933, 2201, 2452
Hpy166II	GTNNAC	8 cut(s)	221, 472, 526, 620, 978, 1156, 2034, 2422
Hpy188I	TCNGA	8 cut(s)	126, 462, 662, 1461, 1891, 1931, 2068, 2463
Hpy8I	GTNNAC	8 cut(s)	221, 472, 526, 620, 978, 1156, 2034, 2422
HpyAV	CCTTC	8 cut(s)	245, 410, 438, 442, 770, 1172, 1393, 1628
HpyCH4III	ACNGT	8 cut(s)	811, 916, 1012, 1273, 1409, 1631, 2440, 2485
HpyCH4IV	ACGT	1 cut(s)	257
HpyF10VI	GCNNNNNNNGC	5 cut(s)	116, 590, 734, 1247, 2267
HpyF3I	CTNAG	4 cut(s)	123, 358, 2271, 2427
HpySE526I	ACGT	1 cut(s)	257
Hsp92I	GRCGYC	1 cut(s)	2400
HspAI	GCGC	2 cut(s)	568, 2374
Kpn2I	TCCGGA	1 cut(s)	2007
Ksp22I	TGATCA	1 cut(s)	1143
KspAI	GTTAAC	1 cut(s)	1156
LmnI	GCTCC	4 cut(s)	725, 1199, 1448, 1660
Lsp1109I	GCAGC	8 cut(s)	809, 1004, 1341, 1480, 1828, 2309, 2401, 2486
LweI	GCATC	3 cut(s)	784, 1756, 2214
MaeI	CTAG	5 cut(s)	56, 320, 326, 771, 1134
MaeII	ACGT	1 cut(s)	257
MaeIII	GTNAC	8 cut(s)	280, 503, 857, 875, 1189, 1959, 2018, 2440
MboII	GAAGA	8 cut(s)	76, 203, 338, 767, 770, 801, 1484, 1808
MfeI	CAATTG	4 cut(s)	1504, 1590, 1836, 2169
MflI	RGATCY	3 cut(s)	316, 1047, 1988
MhlI	GDGCHC	2 cut(s)	1484, 2272
MlsI	TGGCCA	1 cut(s)	402
MluNI	TGGCCA	1 cut(s)	402
MlyI	GAGTC	3 cut(s)	833, 992, 1300
MmeI	TCCRAC	2 cut(s)	1498, 2016
Mox20I	TGGCCA	1 cut(s)	402
Mph1103I	ATGCAT	1 cut(s)	1125
MroI	TCCGGA	1 cut(s)	2007
MroXI	GAANNNNTTC	1 cut(s)	2504
MscI	TGGCCA	1 cut(s)	402
MslI	CAYNNNNRTG	1 cut(s)	812
Msp20I	TGGCCA	1 cut(s)	402
MspA1I	CMGCKG	1 cut(s)	995
MspI	CCGG	3 cut(s)	608, 2008, 2222
MspR9I	CCNGG	5 cut(s)	216, 399, 609, 1538, 2497
MunI	CAATTG	4 cut(s)	1504, 1590, 1836, 2169
MvaI	CCWGG	4 cut(s)	216, 399, 1538, 2497
MvnI	CGCG	2 cut(s)	822, 2382
MwoI	GCNNNNNNNGC	5 cut(s)	116, 590, 734, 1247, 2267
NciI	CCSGG	1 cut(s)	609
NcoI	CCATGG	1 cut(s)	1902
NdeI	CATATG	3 cut(s)	829, 1226, 1243
NheI	GCTAGC	1 cut(s)	770
NlaIV	GGNNCC	4 cut(s)	12, 271, 1990, 2080
NmuCI	GTSAC	7 cut(s)	280, 503, 857, 875, 1189, 1959, 2440
NruI	TCGCGA	1 cut(s)	822
NsbI	TGCGCA	1 cut(s)	2375
NsiI	ATGCAT	1 cut(s)	1125
NspI	RCATGY	4 cut(s)	626, 841, 1323, 2389
PaeR7I	CTCGAG	1 cut(s)	2230
PagI	TCATGA	1 cut(s)	289
PaqCI	CACCTGC	2 cut(s)	1631, 1736
PciI	ACATGT	2 cut(s)	622, 837
PdmI	GAANNNNTTC	1 cut(s)	2504
PfeI	GAWTC	7 cut(s)	929, 985, 1568, 1814, 1892, 2368, 2413
PflMI	CCANNNNNTGG	1 cut(s)	2243
PfoI	TCCNGGA	1 cut(s)	2495
PinAI	ACCGGT	1 cut(s)	2221
PleI	GAGTC	3 cut(s)	832, 992, 1300
PpsI	GAGTC	3 cut(s)	832, 992, 1300
PpuMI	RGGWCCY	1 cut(s)	1292
PscI	ACATGT	2 cut(s)	622, 837
PshBI	ATTAAT	1 cut(s)	435
PsiI	TTATAA	1 cut(s)	1016
Psp5II	RGGWCCY	1 cut(s)	1292
Psp6I	CCWGG	4 cut(s)	214, 397, 1536, 2495
PspFI	CCCAGC	1 cut(s)	47
PspGI	CCWGG	4 cut(s)	214, 397, 1536, 2495
PspN4I	GGNNCC	4 cut(s)	12, 271, 1990, 2080
PspPI	GGNCC	5 cut(s)	270, 845, 970, 1292, 2224
PspPPI	RGGWCCY	1 cut(s)	1292
PsrI	GAACNNNNNNTAC	2 cut(s)	1603, 1635
PstI	CTGCAG	3 cut(s)	1198, 1846, 2471
PstNI	CAGNNNCTG	2 cut(s)	193, 1295
PsuI	RGATCY	3 cut(s)	316, 1047, 1988
PvuII	CAGCTG	1 cut(s)	995
RruI	TCGCGA	1 cut(s)	822
RsaI	GTAC	5 cut(s)	222, 630, 693, 842, 2033
RsaNI	GTAC	5 cut(s)	221, 629, 692, 841, 2032
RseI	CAYNNNNRTG	1 cut(s)	812
Sau96I	GGNCC	5 cut(s)	270, 845, 970, 1292, 2224
SchI	GAGTC	3 cut(s)	833, 992, 1300
ScrFI	CCNGG	5 cut(s)	216, 399, 609, 1538, 2497
SduI	GDGCHC	2 cut(s)	1484, 2272
SfaNI	GCATC	3 cut(s)	784, 1756, 2214
SfcI	CTRYAG	6 cut(s)	1039, 1194, 1203, 1737, 1842, 2467
Sfr274I	CTCGAG	1 cut(s)	2230
SinI	GGWCC	3 cut(s)	270, 1292, 2224
SlaI	CTCGAG	1 cut(s)	2230
SmiMI	CAYNNNNRTG	1 cut(s)	812
SmlI	CTYRAG	1 cut(s)	2230
SmoI	CTYRAG	1 cut(s)	2230
SsiI	CCGC	4 cut(s)	383, 1172, 1232, 1595
SspI	AATATT	1 cut(s)	427
SspMI	CTAG	5 cut(s)	56, 320, 326, 771, 1134
StyD4I	CCNGG	5 cut(s)	214, 397, 607, 1536, 2495
StyI	CCWWGG	2 cut(s)	1093, 1902
TaaI	ACNGT	8 cut(s)	811, 916, 1012, 1273, 1409, 1631, 2440, 2485
TaiI	ACGT	1 cut(s)	260
TaqI	TCGA	5 cut(s)	547, 988, 1440, 1973, 2231
TatI	WGTACW	3 cut(s)	220, 628, 2031
TauI	GCSGC	2 cut(s)	1175, 1598
TfiI	GAWTC	7 cut(s)	929, 985, 1568, 1814, 1892, 2368, 2413
TscAI	CASTG	6 cut(s)	481, 814, 1414, 1636, 1990, 2476
TseFI	GTSAC	7 cut(s)	280, 503, 857, 875, 1189, 1959, 2440
TseI	GCWGC	8 cut(s)	797, 992, 1354, 1493, 1841, 2297, 2389, 2474
Tsp45I	GTSAC	7 cut(s)	280, 503, 857, 875, 1189, 1959, 2440
TspGWI	ACGGA	1 cut(s)	2071
TspRI	CASTG	6 cut(s)	481, 814, 1414, 1636, 1990, 2476
Van91I	CCANNNNNTGG	1 cut(s)	2243
VpaK11BI	GGWCC	3 cut(s)	270, 1292, 2224
VspI	ATTAAT	1 cut(s)	435
XagI	CCTNNNNNAGG	3 cut(s)	1299, 1781, 2494
XapI	RAATTY	3 cut(s)	393, 864, 1381
XbaI	TCTAGA	1 cut(s)	319
XceI	RCATGY	4 cut(s)	626, 841, 1323, 2389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	127
XhoI	CTCGAG	1 cut(s)	2230
XmnI	GAANNNNTTC	1 cut(s)	2504
XspI	CTAG	5 cut(s)	56, 320, 326, 771, 1134
Zsp2I	ATGCAT	1 cut(s)	1125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.