Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002839

Physical Location & Seq

Genomic Coordinates Reverse (-)

8331877 .. 8335531

Gene Structure 3655 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1114900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGAGAATCCTTAGGCCGGAAAGTATCACAACCTTAGTGGGAGGTGGAACAGGACCTGCTACTGGGACTCGTGCAACAACTTGTACACCAGCCCCATTACACATGAAGTTAATGCTGCAAGCAACTGACGACCTGCCTCTAAATTTTGGATTTACTGGGAAGGGGAACACTGCCAAACCCGAAGGGATGCATGATATAGTAAGGGCTGGGGCAATGGGACTGAAGCTGCATGAGGACTGGGGAAGCACTCCTGCTGCAATCGACAATTGTTTGGCTGTTGGAGATCAATATGACATCCAGGTTAACATCCATACAGACACCTTGAATGAATCTGGATTTGTAGAGCATACAATTGCTGCATTTAAAGGAAGAACTATCCATACTTACCACAGTGAAGGTGCAGGTGGGGGCCATGCTCCAGATATAATCAAAGTCTGCGGTGTGAAAAATGTCCTGCCATCATCAACGAATCCCACACGGCCTTTCACCTCAAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTAGCTTTTGCTGAATCAAGAATAAGGGCTGAAACAATTGCTGCAGAAGATATTTTGCACGATATGGGGGCCATTAGCATTGTATCTTCTGATTCACAGGCTATGGGTCGCATTGGAGAGGTGATAATCAGAACTTGGCAAACAGCTGACAAGATGAAATCACAAAGAGGGTCGATAGAACCTAGTGGATCCGACAATGACAACTTCCGGATCAAACGATACATCGCAAAGTACACTATAAATCCAGCAATAGTTAATGGATTTTCTCAGTATGTTGGTTCTATTGAGGTGGGGAAGCTGGCTGATCTTGTTCTATGGAAGCCATCGTTCTTTGGCGCAAAACCAGAAATGGTGATCAAGGGTGGTGCAATTGCATGGGCTAATATGGGTGATCCAAATGCAAGCATCCCCACCCCTGAACCGGTTATTTCAAGGCCTATGTTTGGTGCATTTGGCAAGGCTGGTAGTGCTCATTCCATTGCTTTTGCAGCTGTAGATAACTGCGTTAAAGACTCGTATCGCCTTGACAAGAGAGTGGAAGCCGTGCGCAACGTTAGGAAACTCAGCAAACTCGACATGAAGCTCAATGACGCCCTACCAAATATAGAGGTTGATCCGGAAACATACAAGGTAACAGCAGACGGCGTGGTTCTTACCTGCAGTCCAGCCACCACAGTTCCACTATCTCGGAACTACTTCATGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006807 GO:0008150 GO:0008152 GO:0009039 GO:0016787 GO:0016810 GO:0016811

KEGG Pathways

Metabolic & Signaling

ko00220 ko00230 ko00791 ko01100 ko01120 map00220 map00230 map00791 map01100 map01120

Pfam Domains

Protein Families

Amidohydro_1 Urease_alpha Urease_beta Urease_gamma

Protein Analysis

423

Amino Acids

45.57

Weight (kDa)

6.12

Isoelectric Point (pI)

30.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_1	PF01979	8 - 309	1e-67	Amidohydrolase family
Amidohydro_3	PF07969	89 - 311	3.2e-06	Amidohydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002839)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67550
fragaria_vesca	FvH4_4g24160 FvH4_4g24160 FvH4_4g24160
malus_domestica	MD13G1114100.v1.1 MD13G1114500.v1.1 MD13G1114900.v1.1 MD13G1115100.v1.1 MD16G1113300.v1.1 MD16G1113700.v1.1
prunus_persica	Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1 Prupe.1G232300_v2.0.a1
pyrus_communis	pycom13g09950 pycom16g09630 pycom16g09640 pycom16g09650
rosa_chinensis	RchiOBHm_Chr1g0326041 RchiOBHm_Chr4g0430931
rosa_laevigata	RLG00000006937
rosa_multiflora	Rmu_sc0006022.1_g000001 Rmu_ssc0000176.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223360 Rroxscaffold_5G00372620
rosa_rugosa	Rorug02G0437300 Rorug04G0247300 Rorug05G0134300
rosa_samantha	Rh2BG408000 Rh4AG303000 Rh4AG303900 Rh4BG311200 Rh4CG327500 Rh4DG307300 Rh7CG419200 Rh7CG419300 Rh7CG439900
rosa_wichuraiana	Rw4G026340 Rw7G035140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	392
Acc16I	TGCGCA	1 cut(s)	1109
Acc36I	ACCTGC	4 cut(s)	64, 141, 392, 1226
AccIII	TCCGGA	2 cut(s)	768, 1177
AciI	CCGC	1 cut(s)	438
AclI	AACGTT	1 cut(s)	1113
AclWI	GGATC	5 cut(s)	744, 757, 779, 947, 1169
AcsI	RAATTY	1 cut(s)	142
AcuI	CTGAAG	1 cut(s)	242
AcyI	GRCGYC	1 cut(s)	1152
AfaI	GTAC	2 cut(s)	85, 794
AfiI	CCNNNNNNNGG	5 cut(s)	16, 62, 544, 982, 1004
AgeI	ACCGGT	1 cut(s)	982
AgsI	TTSAA	2 cut(s)	325, 993
AjnI	CCWGG	1 cut(s)	297
AluBI	AGCT	6 cut(s)	226, 566, 707, 859, 1052, 1144
AluI	AGCT	6 cut(s)	226, 566, 707, 859, 1052, 1144
Alw21I	GWGCWC	1 cut(s)	1033
AlwI	GGATC	5 cut(s)	744, 757, 779, 947, 1169
AlwNI	CAGNNNCTG	1 cut(s)	56
Aor13HI	TCCGGA	2 cut(s)	768, 1177
AoxI	GGCC	5 cut(s)	14, 409, 479, 630, 995
ApeKI	GCWGC	6 cut(s)	115, 226, 254, 356, 602, 1049
ApoI	RAATTY	1 cut(s)	142
AsiGI	ACCGGT	1 cut(s)	982
Asp700I	GAANNNNTTC	1 cut(s)	1256
AspLEI	GCGC	2 cut(s)	899, 1110
AspS9I	GGNCC	3 cut(s)	53, 409, 630
AsuHPI	GGTGA	5 cut(s)	478, 527, 694, 925, 962
AvaII	GGWCC	1 cut(s)	53
AxyI	CCTNAGG	1 cut(s)	11
BamHI	GGATCC	1 cut(s)	749
BauI	CACGAG	1 cut(s)	69
Bbv12I	GWGCWC	1 cut(s)	1033
BbvI	GCAGC	6 cut(s)	102, 213, 241, 343, 589, 1061
BccI	CCATC	4 cut(s)	466, 517, 540, 892
BceAI	ACGGC	3 cut(s)	494, 1088, 1219
BciT130I	CCWGG	1 cut(s)	299
BclI	TGATCA	1 cut(s)	915
BfaI	CTAG	1 cut(s)	744
BfmI	CTRYAG	4 cut(s)	498, 603, 1053, 1219
BfuAI	ACCTGC	4 cut(s)	64, 141, 392, 1226
BisI	GCNGC	6 cut(s)	116, 227, 255, 357, 603, 1050
BlsI	GCNGC	6 cut(s)	117, 228, 256, 358, 604, 1051
Bme1390I	CCNGG	1 cut(s)	299
Bme18I	GGWCC	1 cut(s)	53
BmgT120I	GGNCC	3 cut(s)	53, 409, 630
BmiI	GGNNCC	3 cut(s)	410, 631, 751
BmrFI	CCNGG	1 cut(s)	299
BmrI	ACTGGG	3 cut(s)	72, 165, 247
BmsI	GCATC	3 cut(s)	177, 517, 975
BmuI	ACTGGG	3 cut(s)	72, 165, 247
BpmI	CTGGAG	1 cut(s)	402
BsaBI	GATNNNNATC	1 cut(s)	507
BsaHI	GRCGYC	1 cut(s)	1152
BsaWI	WCCGGW	3 cut(s)	768, 982, 1177
Bsc4I	CCNNNNNNNGG	5 cut(s)	16, 62, 544, 982, 1004
Bse118I	RCCGGY	1 cut(s)	982
Bse1I	ACTGG	3 cut(s)	67, 160, 242
Bse21I	CCTNAGG	1 cut(s)	11
Bse3DI	GCAATG	2 cut(s)	219, 1038
Bse8I	GATNNNNATC	1 cut(s)	507
BseAI	TCCGGA	2 cut(s)	768, 1177
BseBI	CCWGG	1 cut(s)	299
BseGI	GGATG	4 cut(s)	192, 294, 306, 966
BseJI	GATNNNNATC	1 cut(s)	507
BseLI	CCNNNNNNNGG	5 cut(s)	16, 62, 544, 982, 1004
BseMI	GCAATG	2 cut(s)	219, 1038
BseMII	CTCAG	2 cut(s)	842, 1138
BseNI	ACTGG	3 cut(s)	67, 160, 242
BseXI	GCAGC	6 cut(s)	102, 213, 241, 343, 589, 1061
BseYI	CCCAGC	1 cut(s)	206
BsgI	GTGCAG	1 cut(s)	420
BshFI	GGCC	5 cut(s)	16, 411, 481, 632, 997
BshTI	ACCGGT	1 cut(s)	982
BsiHKAI	GWGCWC	1 cut(s)	1033
BsiSI	CCGG	4 cut(s)	17, 769, 983, 1178
BslFI	GGGAC	2 cut(s)	79, 231
BslI	CCNNNNNNNGG	5 cut(s)	16, 62, 544, 982, 1004
BsmFI	GGGAC	2 cut(s)	79, 231
BsnI	GGCC	5 cut(s)	16, 411, 481, 632, 997
Bsp1286I	GDGCHC	1 cut(s)	1033
Bsp13I	TCCGGA	2 cut(s)	768, 1177
Bsp1407I	TGTACA	1 cut(s)	83
Bsp143I	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
BspACI	CCGC	1 cut(s)	438
BspANI	GGCC	5 cut(s)	16, 411, 481, 632, 997
BspCNI	CTCAG	2 cut(s)	841, 1137
BspEI	TCCGGA	2 cut(s)	768, 1177
BspLI	GGNNCC	3 cut(s)	410, 631, 751
BspMAI	CTGCAG	2 cut(s)	607, 1223
BspMI	ACCTGC	4 cut(s)	64, 141, 392, 1226
BspPI	GGATC	5 cut(s)	744, 757, 779, 947, 1169
BsrDI	GCAATG	2 cut(s)	219, 1038
BsrFI	RCCGGY	1 cut(s)	982
BsrGI	TGTACA	1 cut(s)	83
BsrI	ACTGG	3 cut(s)	67, 160, 242
BssAI	RCCGGY	1 cut(s)	982
BssMI	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
BssNI	GRCGYC	1 cut(s)	1152
BssSI	CACGAG	1 cut(s)	69
Bst2BI	CACGAG	1 cut(s)	69
Bst2UI	CCWGG	1 cut(s)	299
Bst4CI	ACNGT	2 cut(s)	392, 1237
BstACI	GRCGYC	1 cut(s)	1152
BstAUI	TGTACA	1 cut(s)	83
BstC8I	GCNNGC	3 cut(s)	120, 861, 964
BstDEI	CTNAG	4 cut(s)	11, 34, 828, 1124
BstENI	CCTNNNNNAGG	1 cut(s)	542
BstF5I	GGATG	4 cut(s)	192, 294, 306, 966
BstHHI	GCGC	2 cut(s)	899, 1110
BstKTI	GATC	7 cut(s)	286, 752, 774, 868, 918, 955, 1177
BstMBI	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
BstMWI	GCNNNNNNNGC	2 cut(s)	1028, 1049
BstNI	CCWGG	1 cut(s)	299
BstSCI	CCNGG	1 cut(s)	297
BstSFI	CTRYAG	4 cut(s)	498, 603, 1053, 1219
BstV1I	GCAGC	6 cut(s)	102, 213, 241, 343, 589, 1061
BstX2I	RGATCY	1 cut(s)	749
BstYI	RGATCY	1 cut(s)	749
Bsu36I	CCTNAGG	1 cut(s)	11
BsuRI	GGCC	5 cut(s)	16, 411, 481, 632, 997
BtgZI	GCGATG	1 cut(s)	769
BtsCI	GGATG	4 cut(s)	192, 294, 306, 966
BtsI	GCAGTG	1 cut(s)	168
BtsIMutI	CAGTG	2 cut(s)	168, 397
BveI	ACCTGC	4 cut(s)	64, 141, 392, 1226
Cac8I	GCNNGC	3 cut(s)	120, 861, 964
CaiI	CAGNNNCTG	1 cut(s)	56
CfoI	GCGC	2 cut(s)	899, 1110
Cfr10I	RCCGGY	1 cut(s)	982
Cfr13I	GGNCC	3 cut(s)	53, 409, 630
CseI	GACGC	1 cut(s)	1160
Csp6I	GTAC	2 cut(s)	84, 793
CspAI	ACCGGT	1 cut(s)	982
CspCI	CAANNNNNGTGG	2 cut(s)	18, 53
CviAII	CATG	7 cut(s)	103, 191, 230, 413, 936, 1138, 1261
CviQI	GTAC	2 cut(s)	84, 793
DdeI	CTNAG	4 cut(s)	11, 34, 828, 1124
DpnI	GATC	7 cut(s)	285, 751, 773, 867, 917, 954, 1176
DpnII	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
DraI	TTTAAA	1 cut(s)	364
Eco147I	AGGCCT	1 cut(s)	997
Eco47I	GGWCC	1 cut(s)	53
Eco57I	CTGAAG	1 cut(s)	242
Eco81I	CCTNAGG	1 cut(s)	11
EcoNI	CCTNNNNNAGG	1 cut(s)	542
EcoO109I	RGGNCCY	1 cut(s)	53
EcoRII	CCWGG	1 cut(s)	297
EcoT22I	ATGCAT	1 cut(s)	192
FaeI	CATG	7 cut(s)	106, 194, 233, 416, 939, 1141, 1264
FaqI	GGGAC	2 cut(s)	79, 231
FatI	CATG	7 cut(s)	102, 190, 229, 412, 935, 1137, 1260
FbaI	TGATCA	1 cut(s)	915
Fnu4HI	GCNGC	6 cut(s)	116, 227, 255, 357, 603, 1050
FokI	GGATG	4 cut(s)	199, 281, 293, 953
Fsp4HI	GCNGC	6 cut(s)	116, 227, 255, 357, 603, 1050
FspBI	CTAG	1 cut(s)	744
FspI	TGCGCA	1 cut(s)	1109
GlaI	GCGC	2 cut(s)	898, 1109
GluI	GCNGC	6 cut(s)	116, 227, 255, 357, 603, 1050
GsaI	CCCAGC	1 cut(s)	210
GsuI	CTGGAG	1 cut(s)	402
HaeIII	GGCC	5 cut(s)	16, 411, 481, 632, 997
HapII	CCGG	4 cut(s)	17, 769, 983, 1178
HgaI	GACGC	1 cut(s)	1160
HhaI	GCGC	2 cut(s)	899, 1110
Hin1I	GRCGYC	1 cut(s)	1152
Hin1II	CATG	7 cut(s)	106, 194, 233, 416, 939, 1141, 1264
Hin6I	GCGC	2 cut(s)	897, 1108
HinP1I	GCGC	2 cut(s)	897, 1108
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HinfI	GANTC	7 cut(s)	6, 67, 329, 469, 575, 653, 1073
HpaI	GTTAAC	1 cut(s)	304
HpaII	CCGG	4 cut(s)	17, 769, 983, 1178
HphI	GGTGA	5 cut(s)	478, 527, 694, 925, 962
Hpy166II	GTNNAC	3 cut(s)	86, 304, 795
Hpy188I	TCNGA	4 cut(s)	652, 692, 754, 1251
Hpy188III	TCNNGA	7 cut(s)	333, 419, 512, 554, 579, 769, 1178
Hpy8I	GTNNAC	3 cut(s)	86, 304, 795
HpyAV	CCTTC	3 cut(s)	154, 176, 389
HpyCH4III	ACNGT	2 cut(s)	392, 1237
HpyCH4IV	ACGT	1 cut(s)	1113
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1028, 1049
HpyF3I	CTNAG	4 cut(s)	11, 34, 828, 1124
HpySE526I	ACGT	1 cut(s)	1113
Hsp92I	GRCGYC	1 cut(s)	1152
Hsp92II	CATG	7 cut(s)	106, 194, 233, 416, 939, 1141, 1264
HspAI	GCGC	2 cut(s)	897, 1108
Kpn2I	TCCGGA	2 cut(s)	768, 1177
Ksp22I	TGATCA	1 cut(s)	915
KspAI	GTTAAC	1 cut(s)	304
Kzo9I	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
LmnI	GCTCC	1 cut(s)	421
Lsp1109I	GCAGC	6 cut(s)	102, 213, 241, 343, 589, 1061
LweI	GCATC	3 cut(s)	177, 517, 975
MaeI	CTAG	1 cut(s)	744
MaeII	ACGT	1 cut(s)	1113
MaeIII	GTNAC	1 cut(s)	1192
MalI	GATC	7 cut(s)	285, 751, 773, 867, 917, 954, 1176
MboI	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
MboII	GAAGA	4 cut(s)	381, 569, 620, 639
MfeI	CAATTG	4 cut(s)	265, 351, 597, 930
MflI	RGATCY	1 cut(s)	749
MhlI	GDGCHC	1 cut(s)	1033
MluCI	AATT	5 cut(s)	142, 265, 351, 597, 930
MlyI	GAGTC	2 cut(s)	61, 1067
MmeI	TCCRAC	2 cut(s)	259, 777
MnlI	CCTC	8 cut(s)	35, 147, 226, 499, 673, 722, 841, 1162
Mph1103I	ATGCAT	1 cut(s)	192
MroI	TCCGGA	2 cut(s)	768, 1177
MroXI	GAANNNNTTC	1 cut(s)	1256
MseI	TTAA	5 cut(s)	110, 303, 363, 816, 1068
MspA1I	CMGCKG	2 cut(s)	707, 1052
MspI	CCGG	4 cut(s)	17, 769, 983, 1178
MspR9I	CCNGG	1 cut(s)	299
MunI	CAATTG	4 cut(s)	265, 351, 597, 930
MvaI	CCWGG	1 cut(s)	299
MwoI	GCNNNNNNNGC	2 cut(s)	1028, 1049
NdeII	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
NlaIII	CATG	7 cut(s)	106, 194, 233, 416, 939, 1141, 1264
NlaIV	GGNNCC	3 cut(s)	410, 631, 751
NsbI	TGCGCA	1 cut(s)	1109
NsiI	ATGCAT	1 cut(s)	192
PaqCI	CACCTGC	1 cut(s)	392
PceI	AGGCCT	1 cut(s)	997
PdmI	GAANNNNTTC	1 cut(s)	1256
PfeI	GAWTC	5 cut(s)	6, 329, 469, 575, 653
PinAI	ACCGGT	1 cut(s)	982
PkrI	GCNGC	6 cut(s)	117, 228, 256, 358, 604, 1051
PleI	GAGTC	2 cut(s)	61, 1067
PpsI	GAGTC	2 cut(s)	61, 1067
PpuMI	RGGWCCY	1 cut(s)	53
Psp1406I	AACGTT	1 cut(s)	1113
Psp5II	RGGWCCY	1 cut(s)	53
Psp6I	CCWGG	1 cut(s)	297
PspFI	CCCAGC	1 cut(s)	206
PspGI	CCWGG	1 cut(s)	297
PspN4I	GGNNCC	3 cut(s)	410, 631, 751
PspPI	GGNCC	3 cut(s)	53, 409, 630
PspPPI	RGGWCCY	1 cut(s)	53
PsrI	GAACNNNNNNTAC	2 cut(s)	364, 396
PstI	CTGCAG	2 cut(s)	607, 1223
PstNI	CAGNNNCTG	1 cut(s)	56
PsuI	RGATCY	1 cut(s)	749
PvuII	CAGCTG	2 cut(s)	707, 1052
RsaI	GTAC	2 cut(s)	85, 794
RsaNI	GTAC	2 cut(s)	84, 793
SaqAI	TTAA	5 cut(s)	110, 303, 363, 816, 1068
SatI	GCNGC	6 cut(s)	116, 227, 255, 357, 603, 1050
Sau3AI	GATC	7 cut(s)	283, 749, 771, 865, 915, 952, 1174
Sau96I	GGNCC	3 cut(s)	53, 409, 630
SchI	GAGTC	2 cut(s)	61, 1067
ScrFI	CCNGG	1 cut(s)	299
SduI	GDGCHC	1 cut(s)	1033
SfaNI	GCATC	3 cut(s)	177, 517, 975
SfcI	CTRYAG	4 cut(s)	498, 603, 1053, 1219
SinI	GGWCC	1 cut(s)	53
Sse9I	AATT	5 cut(s)	142, 265, 351, 597, 930
SseBI	AGGCCT	1 cut(s)	997
SsiI	CCGC	1 cut(s)	438
SspMI	CTAG	1 cut(s)	744
StuI	AGGCCT	1 cut(s)	997
StyD4I	CCNGG	1 cut(s)	297
TaaI	ACNGT	2 cut(s)	392, 1237
TaiI	ACGT	1 cut(s)	1116
TaqI	TCGA	3 cut(s)	261, 734, 1134
TasI	AATT	5 cut(s)	142, 265, 351, 597, 930
TatI	WGTACW	2 cut(s)	83, 792
TfiI	GAWTC	5 cut(s)	6, 329, 469, 575, 653
Tru1I	TTAA	5 cut(s)	110, 303, 363, 816, 1068
Tru9I	TTAA	5 cut(s)	110, 303, 363, 816, 1068
TscAI	CASTG	2 cut(s)	175, 397
TseI	GCWGC	6 cut(s)	115, 226, 254, 356, 602, 1049
TspDTI	ATGAA	5 cut(s)	119, 342, 731, 1154, 1249
TspRI	CASTG	2 cut(s)	175, 397
VpaK11BI	GGWCC	1 cut(s)	53
XagI	CCTNNNNNAGG	1 cut(s)	542
XapI	RAATTY	1 cut(s)	142
XmnI	GAANNNNTTC	1 cut(s)	1256
XspI	CTAG	1 cut(s)	744
Zsp2I	ATGCAT	1 cut(s)	192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.