Rh4AG303000
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002839
Physical Location & Seq
Forward (+)
61556433 .. 61562353
5921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG303000.1

Sequence Viewer

Length: 2526 bp
ATGAAGCTGGTTCCAAGAGAGCTTGACAAATTGGGCCTCCACAATGCTGGGTTTCTAGCCCAGAAGAGACTTGCTCGTGGGTTAAGGCTCAATCACCCTGAAGCTGTAGCACTCATAGCCACTCAGATATTGGAGTTTGTTAGAGATGGTGACAAATCTGTGGCAGAGTTGATGGATATTGGGAAACAGCTTCTAGGGAGGAAGCAAGTTCTTCCGGGTGTTCAATATCTATTGGATATGGTGCAGGTTGAGGGGACGTTTCGTGATGGGACCAAGTTAATCACCATTCATGATCCAATTTCTTGTGATAATGGAGATCTGGAACTAGCTTTAAAGAACTCTTTTCTTCCTGTTCCCTCAATTGATAAGTTTTCGGAAATGGTGGATGGTGGAGTTCCTGGTGAAATAATCTTCAAAGGTGGTAATATTGTAATTAATGAAGGAAGGAAGGGAATAATTCTGAAAGTTGTCAATACTGGAGACAGGCCAGTTCAGGTTGGCAGTCACTATCACTTTATTGAGGTCAATCCCCACTTGGTCTTTGATCGAATGAAATCATATGGTATGCGCCTGAATATACCTGCTGGAACAGCCAAGAGATTTGAGCCGGGGGAATGTAAACATGTCGTACTTGTAAGCATTGGAGGTAATAGAGTAATCAGAGGAGGAAATGACATTGTTGATGGTGCAGTACGAAGCTTTGACAGATACCAAGTAGTCAAGGGAGCTTTGGAAGCCAGACGATTTGGGAATGAAGAAGAACCAAATGCTAGGGAAGGTGTTGCAGGAATAGATGCAGCTTTTACCACTGTAATTTCTCATGAGTCATATGCAAACATGTATGGCCCTACTACCGGTGATAAAGTTCGGCTCGGTGATACCAACTTGTTTGCTGAAATTGAAAAAGATTATACTGTTCATGGGGATGAATCTGTATTTGGAGGTGGCAAAGTTATAAGAGACGGAATGGGCCAGTCATCAAGGAGTGAACTAGCTGACTCATTGGACACAGTTATAACTAATGCTGTAATCATTGATTATAGTGGGATCTTCAAGGCAGATATTGGTATTAAAGATCATCATATAGTTGCCCTTGGGAAATCAGGCAATCCAGATGTTATGGATGGTATGGATGGTGCATCTAGCAATATGATCATTGGGGTTAACACAGAGGTCATTGCAGCGGAAGGACAAATTGTGACTGCTGGAGCTATAGACTGTCATGTGCATTTCATATGCCCTCAGTTGGCATATGAAGCAATATCAAGTGGTATCACAACCCTAGTGGGAGGTGGAACAGGACCTGCTACAGGGACTCGTGCAACAACATGCACACCATCTCCATTGCAAATGAAGTTAATGCTGCAATCAACTGATGAAGTGCCTCTAAATTTTGGCTTTACAGGGAAGGGGAACAGTGCCAAACCTGATGGGCTACGCAAGATAATCAAGGCTGGAGCAATGGGTCTGAAGCTGCATGAAGACTGGGGCACAACTCCTGCTGCAATAGACAATTGTTTGAGTGTTGGAGACCAATATGACGTCCAGGTTAATATCCACACGGACACCTTGAATGAATCTGGATTTGTGGAACACACAATCGCTGCATTTAAAGACAGAACTATCCATACTTACCACAGTGAAGGTGCAGGTGGGGGTCATGCTCCAGATATTATCAAAGTATGTGGTGTGGAAAATGTCCTGCCCTCATCAACGAATCCCACACGGCCTTTCACCTCCAATACTATAGATGAGCATCTTGATATGCTGATGGTTTGCCATCACCTTGACAAGGACAATCCAGAAGATGTAGCTTTTGCTGAATCAAGGATAAGAGCTGAAACAATTGCCGCAGAAGATATATTGCATGATATGGGGGCAATTAGCATGGTAGCTTCTGATTCACAGGCTATGGGTCGCATTGGAGAGGTGATAACCAGAACTTGGCAAACTGCTGACAAGATGAAATCACAAAGAGGGTCAATAGAACCTAGTGGATCCAACAATGACAATCTCCGGATCAAGAGATACATTGCAAAGTACACCATAAATCCAGCAATAGCAAATGGATTTTCTAAATATGTTGGTTCCGTTCAGGCGGGCAAGTTCGCTGATCTTGTTCTATGGAAGCCATCTTTCTTTGGGGCAAAACCAGAAATGGTGATCAAAGGTGGTGCAATTGCATGGGCTAATATGGGTGATCCAAACGCAAGCATCCCGACACCTGAACCGGTCATTTCAAGACCTATGTTTGGTGCATTTGGCAAGGCTAGTAGTGCTCATTCCATTGCCTTTGTCAGCAAGGCAGCTAAAGAAAATGGGGTTAAAGCCTTGTATGACCTGAACAAGAGAGTGGAAGCTGTGGGCAATGTTAGGAAGCTCCGCAAACGCGACATGGAGCTCAATGACGCCCTGCCAAATATTAAAGTTGACCCAGAGACATACACGGTGACAGCAGACGGCGAGGTTCTGACCTGCAGTGCAGCCACCACCGTTCCCCTATCCCGGAATTACTTCCTGTTTTAG

Protein Analysis

841

Amino Acids

90.55

Weight (kDa)

5.92

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urease_gamma PF00547 1 - 100 8.2e-42 Urease, gamma subunit
Urease_beta PF00699 133 - 229 5.8e-41 Urease beta subunit
Urease_alpha PF00449 272 - 393 9.1e-53 Urease alpha-subunit, N-terminal domain
Amidohydro_1 PF01979 399 - 725 1.8e-76 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 956, 1016
AarI CACCTGC 1 cut(s) 1640
AatII GACGTC 1 cut(s) 1545
Acc36I ACCTGC 5 cut(s) 235, 589, 1312, 1640, 2483
AccB7I CCANNNNNTGG 1 cut(s) 1944
AccII CGCG 1 cut(s) 2391
AccIII TCCGGA 1 cut(s) 2016
AciI CCGC 4 cut(s) 1184, 1851, 2099, 2383
AclWI GGATC 6 cut(s) 287, 1055, 1992, 2005, 2027, 2195
AcsI RAATTY 1 cut(s) 1390
AcuI CTGAAG 2 cut(s) 120, 1490
AcyI GRCGYC 2 cut(s) 1542, 2409
AfaI GTAC 3 cut(s) 630, 693, 2042
AfiI CCNNNNNNNGG 7 cut(s) 854, 1246, 1310, 1792, 1944, 2252, 2505
AflIII ACRYGT 2 cut(s) 622, 837
AgeI ACCGGT 2 cut(s) 854, 2230
AgsI TTSAA 6 cut(s) 224, 415, 902, 1054, 1573, 2241
AjnI CCWGG 2 cut(s) 397, 1545
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
Alw21I GWGCWC 2 cut(s) 2281, 2403
Alw26I GTCTC 5 cut(s) 61, 474, 954, 1524, 2432
AlwI GGATC 6 cut(s) 287, 1055, 1992, 2005, 2027, 2195
AlwNI CAGNNNCTG 1 cut(s) 1304
Aor13HI TCCGGA 1 cut(s) 2016
AoxI GGCC 5 cut(s) 34, 485, 844, 970, 1727
ApeKI GCWGC 8 cut(s) 797, 1181, 1363, 1474, 1502, 1604, 2306, 2483
ApoI RAATTY 1 cut(s) 1390
AseI ATTAAT 1 cut(s) 435
AsiGI ACCGGT 2 cut(s) 854, 2230
Asp700I GAANNNNTTC 1 cut(s) 2513
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 5 cut(s) 34, 270, 845, 970, 1301
AsuC2I CCSGG 3 cut(s) 216, 609, 2506
AvaII GGWCC 2 cut(s) 270, 1301
BaeGI GKGCMC 1 cut(s) 1493
BamHI GGATCC 1 cut(s) 1997
BanII GRGCYC 1 cut(s) 2403
BauI CACGAG 2 cut(s) 75, 1317
BbsI GAAGAC 1 cut(s) 1488
Bbv12I GWGCWC 2 cut(s) 2281, 2403
BbvI GCAGC 8 cut(s) 809, 1193, 1350, 1461, 1489, 1591, 2318, 2495
BceAI ACGGC 2 cut(s) 1742, 2476
BciT130I CCWGG 2 cut(s) 399, 1547
BclI TGATCA 2 cut(s) 1152, 2163
BcnI CCSGG 3 cut(s) 216, 609, 2506
BcoDI GTCTC 5 cut(s) 61, 474, 954, 1524, 2432
BfaI CTAG 9 cut(s) 56, 194, 326, 771, 992, 1143, 1283, 1992, 2271
BfmI CTRYAG 5 cut(s) 105, 1212, 1308, 1746, 2476
BfuAI ACCTGC 5 cut(s) 235, 589, 1312, 1640, 2483
BglII AGATCT 1 cut(s) 316
BisI GCNGC 9 cut(s) 798, 1182, 1364, 1475, 1503, 1605, 1851, 2307, 2484
BlsI GCNGC 9 cut(s) 799, 1183, 1365, 1476, 1504, 1606, 1852, 2308, 2485
Bme1390I CCNGG 5 cut(s) 216, 399, 609, 1547, 2506
Bme18I GGWCC 2 cut(s) 270, 1301
BmgT120I GGNCC 5 cut(s) 34, 270, 845, 970, 1301
BmiI GGNNCC 4 cut(s) 12, 271, 1999, 2089
BmrFI CCNGG 5 cut(s) 216, 399, 609, 1547, 2506
BmrI ACTGGG 1 cut(s) 1495
BmsI GCATC 4 cut(s) 784, 1148, 1765, 2223
BmuI ACTGGG 1 cut(s) 1495
BpiI GAAGAC 1 cut(s) 1488
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 4 cut(s) 498, 1227, 1476, 1650
BpuMI CCSGG 3 cut(s) 216, 609, 2506
BsaBI GATNNNNATC 1 cut(s) 1755
BsaHI GRCGYC 2 cut(s) 1542, 2409
BsaI GGTCTC 1 cut(s) 1524
BsaJI CCNNGG 2 cut(s) 608, 1093
BsaWI WCCGGW 3 cut(s) 854, 2016, 2230
BsaXI ACNNNNNCTCC 2 cut(s) 1324, 1354
Bsc4I CCNNNNNNNGG 7 cut(s) 854, 1246, 1310, 1792, 1944, 2252, 2505
Bse118I RCCGGY 2 cut(s) 854, 2230
Bse1I ACTGG 4 cut(s) 481, 488, 973, 1490
Bse3DI GCAATG 6 cut(s) 1176, 1343, 1467, 2031, 2286, 2374
Bse8I GATNNNNATC 1 cut(s) 1755
BseAI TCCGGA 1 cut(s) 2016
BseBI CCWGG 2 cut(s) 399, 1547
BseDI CCNNGG 2 cut(s) 608, 1093
BseGI GGATG 5 cut(s) 391, 931, 1129, 1138, 2214
BseJI GATNNNNATC 1 cut(s) 1755
BseLI CCNNNNNNNGG 7 cut(s) 854, 1246, 1310, 1792, 1944, 2252, 2505
BseMI GCAATG 6 cut(s) 1176, 1343, 1467, 2031, 2286, 2374
BseMII CTCAG 2 cut(s) 137, 1256
BseNI ACTGG 4 cut(s) 481, 488, 973, 1490
BseRI GAGGAG 1 cut(s) 678
BseSI GKGCMC 1 cut(s) 1493
BseXI GCAGC 8 cut(s) 809, 1193, 1350, 1461, 1489, 1591, 2318, 2495
BseYI CCCAGC 1 cut(s) 47
BsgI GTGCAG 4 cut(s) 263, 708, 1668, 2502
Bsh1236I CGCG 1 cut(s) 2391
BshFI GGCC 5 cut(s) 36, 487, 846, 972, 1729
BshTI ACCGGT 2 cut(s) 854, 2230
BsiHKAI GWGCWC 2 cut(s) 2281, 2403
BsiSI CCGG 6 cut(s) 215, 608, 855, 2017, 2231, 2506
BslFI GGGAC 3 cut(s) 268, 283, 1327
BslI CCNNNNNNNGG 7 cut(s) 854, 1246, 1310, 1792, 1944, 2252, 2505
BsmAI GTCTC 5 cut(s) 61, 474, 954, 1524, 2432
BsmBI CGTCTC 1 cut(s) 954
BsmFI GGGAC 3 cut(s) 268, 283, 1327
BsnI GGCC 5 cut(s) 36, 487, 846, 972, 1729
Bso31I GGTCTC 1 cut(s) 1524
Bsp1286I GDGCHC 3 cut(s) 1493, 2281, 2403
Bsp13I TCCGGA 1 cut(s) 2016
BspACI CCGC 4 cut(s) 1184, 1851, 2099, 2383
BspANI GGCC 5 cut(s) 36, 487, 846, 972, 1729
BspCNI CTCAG 2 cut(s) 136, 1255
BspEI TCCGGA 1 cut(s) 2016
BspFNI CGCG 1 cut(s) 2391
BspHI TCATGA 2 cut(s) 289, 820
BspLI GGNNCC 4 cut(s) 12, 271, 1999, 2089
BspMAI CTGCAG 1 cut(s) 2480
BspMI ACCTGC 5 cut(s) 235, 589, 1312, 1640, 2483
BspPI GGATC 6 cut(s) 287, 1055, 1992, 2005, 2027, 2195
BspTNI GGTCTC 1 cut(s) 1524
BsrDI GCAATG 6 cut(s) 1176, 1343, 1467, 2031, 2286, 2374
BsrFI RCCGGY 2 cut(s) 854, 2230
BsrI ACTGG 4 cut(s) 481, 488, 973, 1490
BssAI RCCGGY 2 cut(s) 854, 2230
BssECI CCNNGG 2 cut(s) 608, 1093
BssNI GRCGYC 2 cut(s) 1542, 2409
BssSI CACGAG 2 cut(s) 75, 1317
BssT1I CCWWGG 1 cut(s) 1093
Bst2BI CACGAG 2 cut(s) 75, 1317
Bst2UI CCWGG 2 cut(s) 399, 1547
Bst4CI ACNGT 8 cut(s) 811, 916, 1012, 1220, 1418, 1640, 2449, 2494
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 2 cut(s) 1542, 2409
BstC8I GCNNGC 2 cut(s) 2101, 2212
BstDEI CTNAG 2 cut(s) 123, 1242
BstENI CCTNNNNNAGG 2 cut(s) 1308, 1790
BstF5I GGATG 5 cut(s) 391, 931, 1129, 1138, 2214
BstFNI CGCG 1 cut(s) 2391
BstHHI GCGC 1 cut(s) 570
BstMAI GTCTC 5 cut(s) 61, 474, 954, 1524, 2432
BstMWI GCNNNNNNNGC 5 cut(s) 116, 590, 734, 1256, 2276
BstNI CCWGG 2 cut(s) 399, 1547
BstNSI RCATGY 3 cut(s) 626, 841, 1332
BstSCI CCNGG 5 cut(s) 214, 397, 607, 1545, 2504
BstSFI CTRYAG 5 cut(s) 105, 1212, 1308, 1746, 2476
BstSLI GKGCMC 1 cut(s) 1493
BstUI CGCG 1 cut(s) 2391
BstV1I GCAGC 8 cut(s) 809, 1193, 1350, 1461, 1489, 1591, 2318, 2495
BstV2I GAAGAC 1 cut(s) 1488
BstX2I RGATCY 3 cut(s) 316, 1047, 1997
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 3 cut(s) 316, 1047, 1997
BsuRI GGCC 5 cut(s) 36, 487, 846, 972, 1729
BtsCI GGATG 5 cut(s) 391, 931, 1129, 1138, 2214
BtsI GCAGTG 1 cut(s) 2485
BtsIMutI CAGTG 4 cut(s) 807, 1423, 1645, 2485
BveI ACCTGC 5 cut(s) 235, 589, 1312, 1640, 2483
Cac8I GCNNGC 2 cut(s) 2101, 2212
CaiI CAGNNNCTG 1 cut(s) 1304
CciI TCATGA 2 cut(s) 289, 820
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 2 cut(s) 854, 2230
Cfr13I GGNCC 5 cut(s) 34, 270, 845, 970, 1301
CseI GACGC 1 cut(s) 2417
Csp6I GTAC 3 cut(s) 629, 692, 2041
CspAI ACCGGT 2 cut(s) 854, 2230
CspCI CAANNNNNGTGG 4 cut(s) 1266, 1301, 1666, 1701
CviQI GTAC 3 cut(s) 629, 692, 2041
DdeI CTNAG 2 cut(s) 123, 1242
DraI TTTAAA 2 cut(s) 333, 1612
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 2401
Eco130I CCWWGG 1 cut(s) 1093
Eco24I GRGCYC 1 cut(s) 2403
Eco31I GGTCTC 1 cut(s) 1524
Eco47I GGWCC 2 cut(s) 270, 1301
Eco53kI GAGCTC 1 cut(s) 2401
Eco57I CTGAAG 2 cut(s) 120, 1490
EcoICRI GAGCTC 1 cut(s) 2401
EcoNI CCTNNNNNAGG 2 cut(s) 1308, 1790
EcoO109I RGGNCCY 1 cut(s) 1301
EcoRII CCWGG 2 cut(s) 397, 1545
EcoT14I CCWWGG 1 cut(s) 1093
EcoT38I GRGCYC 1 cut(s) 2403
ErhI CCWWGG 1 cut(s) 1093
Esp3I CGTCTC 1 cut(s) 954
FaqI GGGAC 3 cut(s) 268, 283, 1327
FauI CCCGC 1 cut(s) 2092
FauNDI CATATG 4 cut(s) 559, 829, 1235, 1252
FbaI TGATCA 2 cut(s) 1152, 2163
Fnu4HI GCNGC 9 cut(s) 798, 1182, 1364, 1475, 1503, 1605, 1851, 2307, 2484
FokI GGATG 5 cut(s) 398, 938, 1136, 1145, 2201
FriOI GRGCYC 1 cut(s) 2403
Fsp4HI GCNGC 9 cut(s) 798, 1182, 1364, 1475, 1503, 1605, 1851, 2307, 2484
FspBI CTAG 9 cut(s) 56, 194, 326, 771, 992, 1143, 1283, 1992, 2271
GlaI GCGC 1 cut(s) 569
GluI GCNGC 9 cut(s) 798, 1182, 1364, 1475, 1503, 1605, 1851, 2307, 2484
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 4 cut(s) 498, 1227, 1476, 1650
HaeIII GGCC 5 cut(s) 36, 487, 846, 972, 1729
HapII CCGG 6 cut(s) 215, 608, 855, 2017, 2231, 2506
HgaI GACGC 1 cut(s) 2417
HhaI GCGC 1 cut(s) 570
Hin1I GRCGYC 2 cut(s) 1542, 2409
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HincII GTYRAC 2 cut(s) 1165, 2431
HindII GTYRAC 2 cut(s) 1165, 2431
HindIII AAGCTT 1 cut(s) 697
HinfI GANTC 8 cut(s) 824, 929, 998, 1315, 1577, 1717, 1823, 1901
HpaI GTTAAC 1 cut(s) 1165
HpaII CCGG 6 cut(s) 215, 608, 855, 2017, 2231, 2506
Hpy166II GTNNAC 5 cut(s) 620, 989, 1165, 2043, 2431
Hpy188I TCNGA 7 cut(s) 126, 376, 462, 662, 1470, 1900, 2472
Hpy8I GTNNAC 5 cut(s) 620, 989, 1165, 2043, 2431
HpyAV CCTTC 7 cut(s) 434, 438, 442, 770, 1181, 1402, 1637
HpyCH4III ACNGT 8 cut(s) 811, 916, 1012, 1220, 1418, 1640, 2449, 2494
HpyCH4IV ACGT 2 cut(s) 257, 1542
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 590, 734, 1256, 2276
HpyF3I CTNAG 2 cut(s) 123, 1242
HpySE526I ACGT 2 cut(s) 257, 1542
Hsp92I GRCGYC 2 cut(s) 1542, 2409
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 1 cut(s) 2016
Ksp22I TGATCA 2 cut(s) 1152, 2163
KspAI GTTAAC 1 cut(s) 1165
LmnI GCTCC 6 cut(s) 725, 1208, 1457, 1669, 2385, 2398
Lsp1109I GCAGC 8 cut(s) 809, 1193, 1350, 1461, 1489, 1591, 2318, 2495
LweI GCATC 4 cut(s) 784, 1148, 1765, 2223
MaeI CTAG 9 cut(s) 56, 194, 326, 771, 992, 1143, 1283, 1992, 2271
MaeII ACGT 2 cut(s) 257, 1542
MaeIII GTNAC 4 cut(s) 149, 503, 1198, 2449
MfeI CAATTG 4 cut(s) 360, 1513, 1845, 2178
MflI RGATCY 3 cut(s) 316, 1047, 1997
MhlI GDGCHC 3 cut(s) 1493, 2281, 2403
MlyI GAGTC 3 cut(s) 833, 992, 1309
MmeI TCCRAC 2 cut(s) 1507, 2025
MroI TCCGGA 1 cut(s) 2016
MroXI GAANNNNTTC 1 cut(s) 2513
MslI CAYNNNNRTG 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 6 cut(s) 215, 608, 855, 2017, 2231, 2506
MspR9I CCNGG 5 cut(s) 216, 399, 609, 1547, 2506
MunI CAATTG 4 cut(s) 360, 1513, 1845, 2178
MvaI CCWGG 2 cut(s) 399, 1547
MvnI CGCG 1 cut(s) 2391
MwoI GCNNNNNNNGC 5 cut(s) 116, 590, 734, 1256, 2276
NciI CCSGG 3 cut(s) 216, 609, 2506
NdeI CATATG 4 cut(s) 559, 829, 1235, 1252
NlaIV GGNNCC 4 cut(s) 12, 271, 1999, 2089
NmuCI GTSAC 4 cut(s) 149, 503, 1198, 2449
NspI RCATGY 3 cut(s) 626, 841, 1332
PagI TCATGA 2 cut(s) 289, 820
PaqCI CACCTGC 1 cut(s) 1640
PciI ACATGT 2 cut(s) 622, 837
PdmI GAANNNNTTC 1 cut(s) 2513
PfeI GAWTC 5 cut(s) 929, 1577, 1717, 1823, 1901
PflMI CCANNNNNTGG 1 cut(s) 1944
PfoI TCCNGGA 1 cut(s) 2504
PinAI ACCGGT 2 cut(s) 854, 2230
PkrI GCNGC 9 cut(s) 799, 1183, 1365, 1476, 1504, 1606, 1852, 2308, 2485
PleI GAGTC 3 cut(s) 832, 992, 1309
PpsI GAGTC 3 cut(s) 832, 992, 1309
PpuMI RGGWCCY 1 cut(s) 1301
PscI ACATGT 2 cut(s) 622, 837
PshBI ATTAAT 1 cut(s) 435
PsiI TTATAA 2 cut(s) 956, 1016
Psp124BI GAGCTC 1 cut(s) 2403
Psp5II RGGWCCY 1 cut(s) 1301
Psp6I CCWGG 2 cut(s) 397, 1545
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 397, 1545
PspN4I GGNNCC 4 cut(s) 12, 271, 1999, 2089
PspPI GGNCC 5 cut(s) 34, 270, 845, 970, 1301
PspPPI RGGWCCY 1 cut(s) 1301
PsrI GAACNNNNNNTAC 2 cut(s) 1612, 1644
PstI CTGCAG 1 cut(s) 2480
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 3 cut(s) 316, 1047, 1997
RsaI GTAC 3 cut(s) 630, 693, 2042
RsaNI GTAC 3 cut(s) 629, 692, 2041
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 1 cut(s) 2403
SatI GCNGC 9 cut(s) 798, 1182, 1364, 1475, 1503, 1605, 1851, 2307, 2484
Sau96I GGNCC 5 cut(s) 34, 270, 845, 970, 1301
SchI GAGTC 3 cut(s) 833, 992, 1309
ScrFI CCNGG 5 cut(s) 216, 399, 609, 1547, 2506
SduI GDGCHC 3 cut(s) 1493, 2281, 2403
SfaNI GCATC 4 cut(s) 784, 1148, 1765, 2223
SfcI CTRYAG 5 cut(s) 105, 1212, 1308, 1746, 2476
SinI GGWCC 2 cut(s) 270, 1301
SmiMI CAYNNNNRTG 1 cut(s) 924
SsiI CCGC 4 cut(s) 1184, 1851, 2099, 2383
SspI AATATT 2 cut(s) 427, 2422
SspMI CTAG 9 cut(s) 56, 194, 326, 771, 992, 1143, 1283, 1992, 2271
SstI GAGCTC 1 cut(s) 2403
StyD4I CCNGG 5 cut(s) 214, 397, 607, 1545, 2504
StyI CCWWGG 1 cut(s) 1093
TaaI ACNGT 8 cut(s) 811, 916, 1012, 1220, 1418, 1640, 2449, 2494
TaiI ACGT 2 cut(s) 260, 1545
TaqI TCGA 1 cut(s) 547
TatI WGTACW 1 cut(s) 2040
TauI GCSGC 1 cut(s) 1853
TfiI GAWTC 5 cut(s) 929, 1577, 1717, 1823, 1901
TscAI CASTG 4 cut(s) 814, 1423, 1645, 2485
TseFI GTSAC 4 cut(s) 149, 503, 1198, 2449
TseI GCWGC 8 cut(s) 797, 1181, 1363, 1474, 1502, 1604, 2306, 2483
Tsp45I GTSAC 4 cut(s) 149, 503, 1198, 2449
TspGWI ACGGA 3 cut(s) 978, 1577, 2080
TspRI CASTG 4 cut(s) 814, 1423, 1645, 2485
Van91I CCANNNNNTGG 1 cut(s) 1944
VpaK11BI GGWCC 2 cut(s) 270, 1301
VspI ATTAAT 1 cut(s) 435
XagI CCTNNNNNAGG 2 cut(s) 1308, 1790
XapI RAATTY 1 cut(s) 1390
XceI RCATGY 3 cut(s) 626, 841, 1332
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 2513
XspI CTAG 9 cut(s) 56, 194, 326, 771, 992, 1143, 1283, 1992, 2271
ZraI GACGTC 1 cut(s) 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.