MD16G1113300.v1.1
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002839
Physical Location & Seq
Reverse (-)
7937339 .. 7939282
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1113300.v1.1.491

Sequence Viewer

Length: 627 bp
ATGACGTTCACAATTTCTCCCGCGAGCACCCGCCAAGACGTGGGGAACACTGCCAACCCTGAAGCGCTACATGATATAGTAATGGCTGGGGCAATGGTACTGAAGCTGCATGAGGAATGGGGAAGCACTCCTGCTGCAATTGACAATTGTTTGGCTGTTGGAGATCAATATGACATCCAGCTGCTATATTCTTTATTTTTATGTGCAGGTGGGGGCCATGCTCCAGATATAATCAAAGTCTGCGGTGTGAAAAATGCCCTGCCGTCATCAACGAATCCCACGTGGCCTTTCACCTCAAATACTATAGATGAGCATCTTGACATGCTGGACATTCCTGAGGATGTATCTTTTGTTGAATCAAGAATAAGGGCTGAAACAATTGCCGCAGAAGATATTTTGCACAATATGGGGGCAATTAGCATTGTATCGTCTGATTCACAGGCTATGGGTCGCATCGGAGAGGTGGGGAAGCTGGCTGATCTTGTTCTATGGAAGTCATCGTTCTTTGGCACAAAACCAGAAATGGTGATCAGAGGTGGTGCAGTTGCATGGGTGAATATGGGTGATCCAAATGCAAACATCCCCACACCTGAACCGGTAAAGTTTATAGTTTACAACAATTCTTAG

Protein Analysis

209

Amino Acids

22.33

Weight (kDa)

4.56

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 69 - 154 1.7e-11 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 40
AccII CGCG 1 cut(s) 23
AciI CCGC 4 cut(s) 21, 31, 243, 384
AclWI GGATC 1 cut(s) 560
AcuI CTGAAG 2 cut(s) 81, 122
AcvI CACGTG 1 cut(s) 282
AfaI GTAC 1 cut(s) 99
AfeI AGCGCT 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 40
AgeI ACCGGT 1 cut(s) 595
AgsI TTSAA 1 cut(s) 356
AjiI CACGTC 1 cut(s) 40
AluBI AGCT 3 cut(s) 106, 181, 472
AluI AGCT 3 cut(s) 106, 181, 472
Alw21I GWGCWC 1 cut(s) 29
AlwI GGATC 1 cut(s) 560
Aor51HI AGCGCT 1 cut(s) 66
AoxI GGCC 2 cut(s) 214, 284
ApeKI GCWGC 3 cut(s) 106, 134, 181
AsiGI ACCGGT 1 cut(s) 595
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 283, 538, 565, 575
AxyI CCTNAGG 1 cut(s) 336
BbrPI CACGTG 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 3 cut(s) 93, 121, 168
BceAI ACGGC 1 cut(s) 247
BclI TGATCA 1 cut(s) 528
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 107, 135, 182, 384
BlsI GCNGC 4 cut(s) 108, 136, 183, 385
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 215
BmsI GCATC 2 cut(s) 322, 462
BpmI CTGGAG 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse118I RCCGGY 1 cut(s) 595
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 312
BseGI GGATG 3 cut(s) 174, 346, 579
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 93, 121, 168
BseYI CCCAGC 1 cut(s) 86
BsgI GTGCAG 2 cut(s) 225, 561
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 2 cut(s) 216, 286
BshTI ACCGGT 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 40
BsnI GGCC 2 cut(s) 216, 286
Bsp1286I GDGCHC 1 cut(s) 29
Bsp143I GATC 4 cut(s) 163, 478, 528, 565
BspACI CCGC 4 cut(s) 21, 31, 243, 384
BspANI GGCC 2 cut(s) 216, 286
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 215
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 595
BssAI RCCGGY 1 cut(s) 595
BssMI GATC 4 cut(s) 163, 478, 528, 565
BstBAI YACGTR 1 cut(s) 282
BstC8I GCNNGC 2 cut(s) 25, 474
BstDEI CTNAG 2 cut(s) 336, 624
BstF5I GGATG 3 cut(s) 174, 346, 579
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 4 cut(s) 166, 481, 531, 568
BstMBI GATC 4 cut(s) 163, 478, 528, 565
BstNSI RCATGY 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 93, 121, 168
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 2 cut(s) 216, 286
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 3 cut(s) 174, 346, 579
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 2 cut(s) 25, 474
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 98
CspAI ACCGGT 1 cut(s) 595
CviAII CATG 5 cut(s) 71, 110, 218, 322, 549
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 2 cut(s) 336, 624
DpnI GATC 4 cut(s) 165, 480, 530, 567
DpnII GATC 4 cut(s) 163, 478, 528, 565
Eco47III AGCGCT 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 81, 122
Eco72I CACGTG 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 336
FaeI CATG 5 cut(s) 74, 113, 221, 325, 552
FatI CATG 5 cut(s) 70, 109, 217, 321, 548
FauI CCCGC 2 cut(s) 28, 38
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 4 cut(s) 107, 135, 182, 384
FokI GGATG 3 cut(s) 161, 353, 566
Fsp4HI GCNGC 4 cut(s) 107, 135, 182, 384
GlaI GCGC 1 cut(s) 66
GluI GCNGC 4 cut(s) 107, 135, 182, 384
GsaI CCCAGC 1 cut(s) 90
GsuI CTGGAG 1 cut(s) 207
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 2 cut(s) 216, 286
HapII CCGG 1 cut(s) 596
HhaI GCGC 1 cut(s) 67
Hin1II CATG 5 cut(s) 74, 113, 221, 325, 552
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 3 cut(s) 274, 356, 434
HpaII CCGG 1 cut(s) 596
HphI GGTGA 4 cut(s) 283, 538, 565, 575
Hpy166II GTNNAC 2 cut(s) 9, 613
Hpy188I TCNGA 3 cut(s) 433, 458, 533
Hpy188III TCNNGA 4 cut(s) 224, 317, 335, 360
Hpy8I GTNNAC 2 cut(s) 9, 613
HpyCH4IV ACGT 3 cut(s) 5, 39, 281
HpyCH4V TGCA 7 cut(s) 109, 137, 206, 400, 542, 548, 575
HpyF3I CTNAG 2 cut(s) 336, 624
HpySE526I ACGT 3 cut(s) 5, 39, 281
Hsp92II CATG 5 cut(s) 74, 113, 221, 325, 552
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 4 cut(s) 163, 478, 528, 565
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 3 cut(s) 93, 121, 168
LweI GCATC 2 cut(s) 322, 462
MaeII ACGT 3 cut(s) 5, 39, 281
MalI GATC 4 cut(s) 165, 480, 530, 567
MboI GATC 4 cut(s) 163, 478, 528, 565
MboII GAAGA 1 cut(s) 401
MfeI CAATTG 3 cut(s) 138, 145, 378
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 6 cut(s) 12, 138, 145, 378, 414, 619
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 5 cut(s) 106, 304, 331, 454, 527
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 1 cut(s) 596
MunI CAATTG 3 cut(s) 138, 145, 378
MvnI CGCG 1 cut(s) 23
NdeII GATC 4 cut(s) 163, 478, 528, 565
NlaIII CATG 5 cut(s) 74, 113, 221, 325, 552
NlaIV GGNNCC 1 cut(s) 215
NspI RCATGY 1 cut(s) 325
PaqCI CACCTGC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PfeI GAWTC 3 cut(s) 274, 356, 434
PflMI CCANNNNNTGG 1 cut(s) 40
PinAI ACCGGT 1 cut(s) 595
PkrI GCNGC 4 cut(s) 108, 136, 183, 385
PmaCI CACGTG 1 cut(s) 282
PmlI CACGTG 1 cut(s) 282
Ppu21I YACGTR 1 cut(s) 282
PspCI CACGTG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SatI GCNGC 4 cut(s) 107, 135, 182, 384
Sau3AI GATC 4 cut(s) 163, 478, 528, 565
Sau96I GGNCC 1 cut(s) 214
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 2 cut(s) 322, 462
SfcI CTRYAG 1 cut(s) 303
Sse9I AATT 6 cut(s) 12, 138, 145, 378, 414, 619
SsiI CCGC 4 cut(s) 21, 31, 243, 384
TaiI ACGT 3 cut(s) 8, 42, 284
TasI AATT 6 cut(s) 12, 138, 145, 378, 414, 619
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 3 cut(s) 274, 356, 434
TscAI CASTG 1 cut(s) 55
TseI GCWGC 3 cut(s) 106, 134, 181
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 40
XceI RCATGY 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.