MD13G1114100.v1.1
ERF Family

belongs to the metallo- dependent hydrolases superfamily. Urease alpha subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002839
Physical Location & Seq
Reverse (-)
8272816 .. 8281046
8231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1114100.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGTTTCAAGGACATTGCGAGAGAAGTGCTAGGGTTTTGGAGATTTTCTACTTGAAGGGGAACACTGCCAAACCCGAAGGGATGCATGGTATATTAAGGGCTGGGGCGATGGGACTGAAGCTGCATGAGGACTGGGGAAGCACTCCTGCCGCAATCGACAACTGTTTGGCTGTTGGAGATCAATATGACATCCAGGTTAACATCCATACAGACACCTTGAATGAGTCTGGATTTGTAGAGCATACAATTGCTGCATTTAAAAGAAGAACTATCCATACTTACCAAGGTGAAGGAGCAGGTGGGGGCCATGCTCCAGATATAATCAGAGTCTGTGGCGTGAAAAATGTCCTGCCGTCATCAACGAATCCCACGCGGCCTTTCACCTCAAATACTATAGATGAGCATCTTGACATACTGACGGTTTGCCATCACCTTGACAAGGACATTCCAGAAGATGTGGGGAAGCTGGCTGATCTTGTTCTATGGAAGCCGTCGTTCTTTGGCGCAAAACCAGAAATGGTGATCAAAGGTGGTGCAATTGCATGGGCTAATATGCGTGATCCAAATGCAAGCACATCACCACATCTGATGTCACATCCCGGCCCCGGGTCCACCACATCTCAGGCCCGCTCCATCACCGTAGCACGATATTTCTCTGCCATTTGTTTGTGGGTGTATCATCAGCCTCGGCGACGAACTCCTGCGAGAGCCGTTGAGTTCGAAATTTGCTGGGTTATTTCAAGGCCTCTGTTTGGTGCATTTGGCAAGGCTAGTAGTGCGCATTCCATTGCTTTTGCAGCTGTAGATAATCGGGTTAAAGACTTGTATCGCCGACGCCTTGACAAGAAAGTAGAAGCCGTGTGCAACGTTAGGAAACTCAGCAAACTCGACATGAAGCTCAATGACGCCCTACCAAAAATAAGGTCGGAACTATTTCATGTTTTAGTCTCCTTCATAGTTCCAATGAACCACCTCTCCCACATGTATTTTAATTCAAGTCTAACGAACTCGAATAAACACCGCAAATACGTACAAATAGAACAAGTTATATACTTTAAATAA

Protein Analysis

354

Amino Acids

39.46

Weight (kDa)

9.25

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 37 - 144 2.4e-19 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 287
Acc16I TGCGCA 1 cut(s) 780
Acc36I ACCTGC 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 630
AccII CGCG 1 cut(s) 373
AciI CCGC 4 cut(s) 150, 373, 628, 1021
AclI AACGTT 1 cut(s) 867
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 1 cut(s) 723
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 2 cut(s) 835, 906
AfaI GTAC 1 cut(s) 1032
AfiI CCNNNNNNNGG 4 cut(s) 439, 605, 606, 752
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 5 cut(s) 8, 55, 220, 741, 996
AjnI CCWGG 1 cut(s) 192
AloI GAACNNNNNNTCC 2 cut(s) 959, 991
AluBI AGCT 4 cut(s) 121, 466, 800, 898
AluI AGCT 4 cut(s) 121, 466, 800, 898
Alw26I GTCTC 1 cut(s) 952
AlwI GGATC 1 cut(s) 554
AlwNI CAGNNNCTG 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 5 cut(s) 304, 374, 601, 624, 743
ApeKI GCWGC 3 cut(s) 121, 251, 797
ApoI RAATTY 1 cut(s) 723
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 2 cut(s) 506, 781
AspS9I GGNCC 4 cut(s) 304, 602, 609, 625
AsuC2I CCSGG 3 cut(s) 600, 606, 607
AsuHPI GGTGA 6 cut(s) 299, 373, 422, 532, 570, 628
AsuII TTCGAA 1 cut(s) 720
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 1 cut(s) 609
BbvI GCAGC 3 cut(s) 108, 238, 809
BccI CCATC 3 cut(s) 103, 435, 641
BceAI ACGGC 4 cut(s) 337, 475, 695, 842
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciT130I CCWGG 1 cut(s) 194
BclI TGATCA 1 cut(s) 522
BcnI CCSGG 3 cut(s) 600, 606, 607
BcoDI GTCTC 1 cut(s) 952
BfaI CTAG 2 cut(s) 30, 771
BfmI CTRYAG 2 cut(s) 393, 801
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 5 cut(s) 122, 150, 252, 374, 798
BlsI GCNGC 5 cut(s) 123, 151, 253, 375, 799
Bme1390I CCNGG 4 cut(s) 194, 600, 606, 607
Bme18I GGWCC 1 cut(s) 609
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 4 cut(s) 304, 602, 609, 625
BmiI GGNNCC 3 cut(s) 305, 604, 610
BmrFI CCNGG 4 cut(s) 194, 600, 606, 607
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 72, 412
BmuI ACTGGG 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 720
BpuMI CCSGG 3 cut(s) 600, 606, 607
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 2 cut(s) 835, 906
BsaJI CCNNGG 4 cut(s) 283, 604, 605, 686
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 4 cut(s) 439, 605, 606, 752
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 13, 786
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 4 cut(s) 283, 604, 605, 686
BseGI GGATG 4 cut(s) 87, 189, 201, 595
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 439, 605, 606, 752
BseMI GCAATG 2 cut(s) 13, 786
BseMII CTCAG 2 cut(s) 635, 892
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 108, 238, 809
BseYI CCCAGC 2 cut(s) 101, 729
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 5 cut(s) 306, 376, 603, 626, 745
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 2 cut(s) 600, 606
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 4 cut(s) 439, 605, 606, 752
BsmAI GTCTC 1 cut(s) 952
BsmFI GGGAC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 5 cut(s) 306, 376, 603, 626, 745
BsoBI CYCGRG 1 cut(s) 605
Bsp119I TTCGAA 1 cut(s) 720
Bsp143I GATC 4 cut(s) 178, 472, 522, 559
BspACI CCGC 4 cut(s) 150, 373, 628, 1021
BspANI GGCC 5 cut(s) 306, 376, 603, 626, 745
BspCNI CTCAG 2 cut(s) 634, 891
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 305, 604, 610
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 554
BspT104I TTCGAA 1 cut(s) 720
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 2 cut(s) 13, 786
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 4 cut(s) 283, 604, 605, 686
BssMI GATC 4 cut(s) 178, 472, 522, 559
BssNI GRCGYC 2 cut(s) 835, 906
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 164, 421, 640
BstACI GRCGYC 2 cut(s) 835, 906
BstBAI YACGTR 1 cut(s) 1030
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 3 cut(s) 468, 571, 628
BstDEI CTNAG 2 cut(s) 621, 878
BstENI CCTNNNNNAGG 1 cut(s) 437
BstF5I GGATG 4 cut(s) 87, 189, 201, 595
BstFNI CGCG 1 cut(s) 373
BstHHI GCGC 2 cut(s) 506, 781
BstKTI GATC 4 cut(s) 181, 475, 525, 562
BstMAI GTCTC 1 cut(s) 952
BstMBI GATC 4 cut(s) 178, 472, 522, 559
BstMWI GCNNNNNNNGC 2 cut(s) 776, 797
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 4 cut(s) 192, 598, 604, 605
BstSFI CTRYAG 2 cut(s) 393, 801
BstSNI TACGTA 1 cut(s) 1030
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 3 cut(s) 108, 238, 809
BsuRI GGCC 5 cut(s) 306, 376, 603, 626, 745
BtgZI GCGATG 1 cut(s) 122
BtsCI GGATG 4 cut(s) 87, 189, 201, 595
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 3 cut(s) 468, 571, 628
CaiI CAGNNNCTG 1 cut(s) 330
CfoI GCGC 2 cut(s) 506, 781
Cfr13I GGNCC 4 cut(s) 304, 602, 609, 625
Cfr9I CCCGGG 1 cut(s) 605
CseI GACGC 2 cut(s) 843, 914
Csp6I GTAC 1 cut(s) 1031
CviAII CATG 7 cut(s) 86, 125, 308, 543, 892, 938, 982
CviQI GTAC 1 cut(s) 1031
DdeI CTNAG 2 cut(s) 621, 878
DpnI GATC 4 cut(s) 180, 474, 524, 561
DpnII GATC 4 cut(s) 178, 472, 522, 559
DraI TTTAAA 2 cut(s) 259, 1057
Eco105I TACGTA 1 cut(s) 1030
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 7 cut(s) 89, 128, 311, 546, 895, 941, 985
FaqI GGGAC 1 cut(s) 126
FatI CATG 7 cut(s) 85, 124, 307, 542, 891, 937, 981
FauI CCCGC 1 cut(s) 635
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 5 cut(s) 122, 150, 252, 374, 798
FokI GGATG 4 cut(s) 94, 176, 188, 582
Fsp4HI GCNGC 5 cut(s) 122, 150, 252, 374, 798
FspAI RTGCGCAY 1 cut(s) 780
FspBI CTAG 2 cut(s) 30, 771
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 2 cut(s) 505, 780
GluI GCNGC 5 cut(s) 122, 150, 252, 374, 798
GsaI CCCAGC 2 cut(s) 105, 733
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 5 cut(s) 306, 376, 603, 626, 745
HapII CCGG 2 cut(s) 600, 606
HgaI GACGC 2 cut(s) 843, 914
HhaI GCGC 2 cut(s) 506, 781
Hin1I GRCGYC 2 cut(s) 835, 906
Hin1II CATG 7 cut(s) 89, 128, 311, 546, 895, 941, 985
Hin6I GCGC 2 cut(s) 504, 779
HinP1I GCGC 2 cut(s) 504, 779
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 3 cut(s) 224, 327, 364
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 2 cut(s) 600, 606
HphI GGTGA 6 cut(s) 299, 373, 422, 532, 570, 628
Hpy166II GTNNAC 2 cut(s) 199, 612
Hpy188I TCNGA 3 cut(s) 326, 588, 928
Hpy188III TCNNGA 4 cut(s) 228, 314, 407, 449
Hpy8I GTNNAC 2 cut(s) 199, 612
Hpy99I CGWCG 3 cut(s) 496, 696, 837
HpyAV CCTTC 4 cut(s) 49, 71, 284, 961
HpyCH4III ACNGT 3 cut(s) 164, 421, 640
HpyCH4IV ACGT 2 cut(s) 867, 1029
HpyCH4V TGCA 9 cut(s) 85, 124, 254, 536, 542, 569, 758, 797, 864
HpyF10VI GCNNNNNNNGC 2 cut(s) 776, 797
HpyF3I CTNAG 2 cut(s) 621, 878
HpySE526I ACGT 2 cut(s) 867, 1029
Hsp92I GRCGYC 2 cut(s) 835, 906
Hsp92II CATG 7 cut(s) 89, 128, 311, 546, 895, 941, 985
HspAI GCGC 2 cut(s) 504, 779
Ksp22I TGATCA 1 cut(s) 522
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 4 cut(s) 178, 472, 522, 559
LmnI GCTCC 3 cut(s) 293, 316, 635
Lsp1109I GCAGC 3 cut(s) 108, 238, 809
LweI GCATC 2 cut(s) 72, 412
MaeI CTAG 2 cut(s) 30, 771
MaeII ACGT 2 cut(s) 867, 1029
MaeIII GTNAC 1 cut(s) 591
MalI GATC 4 cut(s) 180, 474, 524, 561
MbiI CCGCTC 1 cut(s) 630
MboI GATC 4 cut(s) 178, 472, 522, 559
MboII GAAGA 2 cut(s) 276, 464
MfeI CAATTG 2 cut(s) 246, 537
MluCI AATT 4 cut(s) 246, 537, 723, 991
MlyI GAGTC 2 cut(s) 233, 336
MmeI TCCRAC 2 cut(s) 154, 906
MnlI CCTC 5 cut(s) 121, 394, 696, 756, 983
Mph1103I ATGCAT 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 6 cut(s) 95, 198, 258, 816, 990, 1056
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 2 cut(s) 600, 606
MspR9I CCNGG 4 cut(s) 194, 600, 606, 607
MunI CAATTG 2 cut(s) 246, 537
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 373
MwoI GCNNNNNNNGC 2 cut(s) 776, 797
NciI CCSGG 3 cut(s) 600, 606, 607
NdeII GATC 4 cut(s) 178, 472, 522, 559
NlaIII CATG 7 cut(s) 89, 128, 311, 546, 895, 941, 985
NlaIV GGNNCC 3 cut(s) 305, 604, 610
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 1 cut(s) 591
NsbI TGCGCA 1 cut(s) 780
NsiI ATGCAT 1 cut(s) 87
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 1 cut(s) 720
PaqCI CACCTGC 1 cut(s) 287
PceI AGGCCT 1 cut(s) 745
PciI ACATGT 1 cut(s) 981
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 5 cut(s) 123, 151, 253, 375, 799
PleI GAGTC 2 cut(s) 232, 335
PpsI GAGTC 2 cut(s) 232, 335
Ppu21I YACGTR 1 cut(s) 1030
PscI ACATGT 1 cut(s) 981
Psp1406I AACGTT 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 192
PspFI CCCAGC 2 cut(s) 101, 729
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 305, 604, 610
PspPI GGNCC 4 cut(s) 304, 602, 609, 625
PsrI GAACNNNNNNTAC 2 cut(s) 259, 291
PstNI CAGNNNCTG 1 cut(s) 330
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 1 cut(s) 1032
RsaNI GTAC 1 cut(s) 1031
SaqAI TTAA 6 cut(s) 95, 198, 258, 816, 990, 1056
SatI GCNGC 5 cut(s) 122, 150, 252, 374, 798
Sau3AI GATC 4 cut(s) 178, 472, 522, 559
Sau96I GGNCC 4 cut(s) 304, 602, 609, 625
SchI GAGTC 2 cut(s) 233, 336
ScrFI CCNGG 4 cut(s) 194, 600, 606, 607
SfaNI GCATC 2 cut(s) 72, 412
SfcI CTRYAG 2 cut(s) 393, 801
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 1 cut(s) 609
SmaI CCCGGG 1 cut(s) 607
SnaBI TACGTA 1 cut(s) 1030
Sse9I AATT 4 cut(s) 246, 537, 723, 991
SseBI AGGCCT 1 cut(s) 745
SsiI CCGC 4 cut(s) 150, 373, 628, 1021
SspMI CTAG 2 cut(s) 30, 771
StuI AGGCCT 1 cut(s) 745
StyD4I CCNGG 4 cut(s) 192, 598, 604, 605
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 164, 421, 640
TaiI ACGT 2 cut(s) 870, 1032
TaqI TCGA 4 cut(s) 156, 720, 888, 1010
TasI AATT 4 cut(s) 246, 537, 723, 991
TauI GCSGC 2 cut(s) 152, 376
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 6 cut(s) 95, 198, 258, 816, 990, 1056
Tru9I TTAA 6 cut(s) 95, 198, 258, 816, 990, 1056
TscAI CASTG 1 cut(s) 70
TseFI GTSAC 1 cut(s) 591
TseI GCWGC 3 cut(s) 121, 251, 797
Tsp45I GTSAC 1 cut(s) 591
TspDTI ATGAA 4 cut(s) 908, 926, 943, 980
TspMI CCCGGG 1 cut(s) 605
TspRI CASTG 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 609
XagI CCTNNNNNAGG 1 cut(s) 437
XapI RAATTY 1 cut(s) 723
XceI RCATGY 1 cut(s) 985
XmaI CCCGGG 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 2 cut(s) 30, 771
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.