AT1G77200

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003146
Physical Location & Seq
Reverse (-)
29004163 .. 29005281
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77200.1

Sequence Viewer

Length: 735 bp
ATGACCGAGTCATCCATTATCTCCGTCAAACAATCGTCTCCGGTGCCGGAGGAGGAGGATCATCATCATCACCAACAAGACTCCCACCGAACCAACACCAAGAAACGTGTAAGATCCGACCCGGGTTACCGAGGCGTCCGGATGCGAACGTGGGGCAAATGGGTCTCCGAGATCCGAGAGCCTCGCAAGAAGAGCCGAATCTGGCTAGGCACTTTCTCCACACCGGAAATGGCGGCGCGTGCACATGACGCAGCTGCTCTCACCATTAAAGGAACTTCCGCCGTCCTCAACTTCCCGGAGCTCGCCACTTACCTTCCCCGTCCAGCTTCCTCCTCTCCACGCGACGTTCAAGCCGCAGCCGCCGTAGCGGCCGCCATGGACTTCTCCCCCTCCTCCTCCTCTCTCGTAGTCTCCGATCCAACCACCGTCATCGCCCCAGCTGAGACGCAGTTGTCTTCCTCGAGCTATTCAACGTGTACGTCATCGTCGTTGTCACCGTCAAGCGAAGAAGCTGCGTCGACGGCGGAAGAACTAAGCGAGATAGTGGAGTTACCGAGTCTAGAGACGAGTTACGATGAGTCATTGAGCGAGTTTGTGTACGTTGACTCGGCGTATCCACCTAGTTCGCCTTGGTATATTAATAATTGTTATAGCTTTTATTACCACAGCGACGAAAATGGCATTTCGATGGCTGAACCTTTCGACTCTTCCAATTTTGGCCCCCTTTTTCCTTAG

Protein Analysis

244

Amino Acids

26.73

Weight (kDa)

4.99

Isoelectric Point (pI)

67.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 41 - 91 4.8e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 518
AccII CGCG 2 cut(s) 238, 342
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 7 cut(s) 233, 279, 354, 360, 368, 372, 524
AclWI GGATC 4 cut(s) 66, 108, 166, 410
AcoI YGGCCR 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 2 cut(s) 478, 599
AflIII ACRYGT 2 cut(s) 106, 473
AgsI TTSAA 2 cut(s) 350, 471
AluBI AGCT 7 cut(s) 254, 301, 326, 440, 465, 512, 654
AluI AGCT 7 cut(s) 254, 301, 326, 440, 465, 512, 654
Alw21I GWGCWC 2 cut(s) 244, 303
Alw26I GTCTC 5 cut(s) 42, 169, 415, 437, 557
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 4 cut(s) 66, 108, 166, 410
Ama87I CYCGRG 2 cut(s) 121, 460
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 2 cut(s) 369, 718
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 4 cut(s) 251, 254, 356, 512
AseI ATTAAT 1 cut(s) 639
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 3 cut(s) 122, 123, 296
AsuHPI GGTGA 3 cut(s) 62, 253, 486
AvaI CYCGRG 2 cut(s) 121, 460
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 244, 303
BbvI GCAGC 4 cut(s) 241, 263, 368, 499
BccI CCATC 1 cut(s) 682
BceAI ACGGC 3 cut(s) 266, 347, 537
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciVI GTATCC 1 cut(s) 624
BcnI CCSGG 3 cut(s) 122, 123, 296
BcoDI GTCTC 5 cut(s) 42, 169, 415, 437, 557
BfaI CTAG 3 cut(s) 206, 560, 621
BfuI GTATCC 1 cut(s) 624
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 9 cut(s) 234, 252, 255, 354, 357, 360, 369, 372, 513
BlsI GCNGC 9 cut(s) 235, 253, 256, 355, 358, 361, 370, 373, 514
Bme1390I CCNGG 3 cut(s) 122, 123, 296
BmeT110I CYCGRG 2 cut(s) 121, 460
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 2 cut(s) 45, 721
BmrFI CCNGG 3 cut(s) 122, 123, 296
BmsI GCATC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 447
BpuMI CCSGG 3 cut(s) 122, 123, 296
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 4 cut(s) 121, 130, 375, 629
BsaWI WCCGGW 3 cut(s) 40, 138, 223
Bse8I GATNNNNATC 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 138
BseDI CCNNGG 4 cut(s) 121, 130, 375, 629
BseGI GGATG 2 cut(s) 11, 147
BseJI GATNNNNATC 1 cut(s) 63
BseMII CTCAG 1 cut(s) 432
BseRI GAGGAG 6 cut(s) 65, 68, 322, 382, 385, 388
BseSI GKGCMC 1 cut(s) 244
BseX3I CGGCCG 1 cut(s) 369
BseXI GCAGC 4 cut(s) 241, 263, 368, 499
BseYI CCCAGC 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 238, 342
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 2 cut(s) 371, 720
BshNI GGYRCC 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 2 cut(s) 244, 303
BsiHKCI CYCGRG 2 cut(s) 121, 460
BsiSI CCGG 6 cut(s) 41, 47, 122, 139, 224, 296
BsmAI GTCTC 5 cut(s) 42, 169, 415, 437, 557
BsmBI CGTCTC 3 cut(s) 42, 437, 557
BsnI GGCC 2 cut(s) 371, 720
Bso31I GGTCTC 1 cut(s) 169
BsoBI CYCGRG 2 cut(s) 121, 460
Bsp1286I GDGCHC 2 cut(s) 244, 303
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 4 cut(s) 58, 113, 171, 415
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 7 cut(s) 233, 279, 354, 360, 368, 372, 524
BspANI GGCC 2 cut(s) 371, 720
BspCNI CTCAG 1 cut(s) 433
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 2 cut(s) 238, 342
BspLI GGNNCC 2 cut(s) 45, 721
BspPI GGATC 4 cut(s) 66, 108, 166, 410
BspQI GCTCTTC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 121, 130, 375, 629
BssMI GATC 4 cut(s) 58, 113, 171, 415
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 375, 629
Bst4CI ACNGT 2 cut(s) 427, 498
Bst6I CTCTTC 2 cut(s) 185, 712
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 240, 303
BstDEI CTNAG 3 cut(s) 441, 533, 732
BstDSI CCRYGG 1 cut(s) 375
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 2 cut(s) 11, 147
BstFNI CGCG 2 cut(s) 238, 342
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 4 cut(s) 61, 116, 174, 418
BstMAI GTCTC 5 cut(s) 42, 169, 415, 437, 557
BstMBI GATC 4 cut(s) 58, 113, 171, 415
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 7 cut(s) 192, 239, 248, 359, 365, 368, 521
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 3 cut(s) 120, 121, 294
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 2 cut(s) 238, 342
BstV1I GCAGC 4 cut(s) 241, 263, 368, 499
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 2 cut(s) 113, 171
BstYI RGATCY 2 cut(s) 113, 171
BstZI CGGCCG 1 cut(s) 369
BsuI GTATCC 1 cut(s) 624
BsuRI GGCC 2 cut(s) 371, 720
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 1 cut(s) 415
BtsCI GGATG 2 cut(s) 11, 147
Cac8I GCNNGC 2 cut(s) 240, 303
CciNI GCGGCCGC 1 cut(s) 369
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 719
Cfr9I CCCGGG 1 cut(s) 121
CseI GACGC 4 cut(s) 124, 257, 454, 504
Csp6I GTAC 2 cut(s) 477, 598
CviAII CATG 2 cut(s) 245, 376
CviQI GTAC 2 cut(s) 477, 598
DdeI CTNAG 3 cut(s) 441, 533, 732
DpnI GATC 4 cut(s) 60, 115, 173, 417
DpnII GATC 4 cut(s) 58, 113, 171, 415
DrdI GACNNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 369
EagI CGGCCG 1 cut(s) 369
Eam1104I CTCTTC 2 cut(s) 185, 712
EarI CTCTTC 2 cut(s) 185, 712
EciI GGCGGA 2 cut(s) 268, 539
Ecl136II GAGCTC 1 cut(s) 301
EclXI CGGCCG 1 cut(s) 369
Eco130I CCWWGG 2 cut(s) 375, 629
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 169
Eco52I CGGCCG 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 301
Eco88I CYCGRG 2 cut(s) 121, 460
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 125
EcoT14I CCWWGG 2 cut(s) 375, 629
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 375, 629
Esp3I CGTCTC 3 cut(s) 42, 437, 557
FaeI CATG 2 cut(s) 248, 379
FaiI YATR 4 cut(s) 246, 377, 636, 651
FatI CATG 2 cut(s) 244, 375
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 9 cut(s) 234, 252, 255, 354, 357, 360, 369, 372, 513
FokI GGATG 1 cut(s) 154
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 9 cut(s) 234, 252, 255, 354, 357, 360, 369, 372, 513
FspBI CTAG 3 cut(s) 206, 560, 621
GlaI GCGC 1 cut(s) 237
GluI GCNGC 9 cut(s) 234, 252, 255, 354, 357, 360, 369, 372, 513
GsaI CCCAGC 1 cut(s) 440
HaeIII GGCC 2 cut(s) 371, 720
HapII CCGG 6 cut(s) 41, 47, 122, 139, 224, 296
HgaI GACGC 4 cut(s) 124, 257, 454, 504
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 2 cut(s) 248, 379
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HincII GTYRAC 2 cut(s) 519, 604
HindII GTYRAC 2 cut(s) 519, 604
HinfI GANTC 7 cut(s) 8, 80, 198, 556, 578, 605, 704
HpaII CCGG 6 cut(s) 41, 47, 122, 139, 224, 296
HphI GGTGA 3 cut(s) 62, 253, 486
Hpy166II GTNNAC 5 cut(s) 242, 477, 519, 598, 604
Hpy188I TCNGA 4 cut(s) 118, 169, 176, 415
Hpy188III TCNNGA 2 cut(s) 139, 560
Hpy8I GTNNAC 5 cut(s) 242, 477, 519, 598, 604
Hpy99I CGWCG 5 cut(s) 347, 490, 520, 523, 674
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 2 cut(s) 427, 498
HpyCH4IV ACGT 6 cut(s) 106, 149, 345, 473, 479, 600
HpyCH4V TGCA 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 7 cut(s) 192, 239, 248, 359, 365, 368, 521
HpyF3I CTNAG 3 cut(s) 441, 533, 732
HpySE526I ACGT 6 cut(s) 106, 149, 345, 473, 479, 600
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 2 cut(s) 248, 379
HspAI GCGC 1 cut(s) 236
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 58, 113, 171, 415
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 9 cut(s) 54, 60, 135, 152, 187, 237, 309, 336, 450
Lsp1109I GCAGC 4 cut(s) 241, 263, 368, 499
LweI GCATC 1 cut(s) 132
MaeI CTAG 3 cut(s) 206, 560, 621
MaeII ACGT 6 cut(s) 106, 149, 345, 473, 479, 600
MaeIII GTNAC 4 cut(s) 125, 492, 549, 569
MalI GATC 4 cut(s) 60, 115, 173, 417
MboI GATC 4 cut(s) 58, 113, 171, 415
MboII GAAGA 5 cut(s) 202, 447, 518, 539, 699
MflI RGATCY 2 cut(s) 113, 171
MhlI GDGCHC 2 cut(s) 244, 303
MluCI AATT 2 cut(s) 643, 712
MlyI GAGTC 6 cut(s) 17, 74, 565, 587, 599, 698
MmeI TCCRAC 2 cut(s) 141, 443
MroI TCCGGA 1 cut(s) 138
MseI TTAA 2 cut(s) 267, 639
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 254, 440
MspI CCGG 6 cut(s) 41, 47, 122, 139, 224, 296
MspR9I CCNGG 3 cut(s) 122, 123, 296
MvnI CGCG 2 cut(s) 238, 342
MwoI GCNNNNNNNGC 7 cut(s) 192, 239, 248, 359, 365, 368, 521
NciI CCSGG 3 cut(s) 122, 123, 296
NcoI CCATGG 1 cut(s) 375
NdeII GATC 4 cut(s) 58, 113, 171, 415
NlaIII CATG 2 cut(s) 248, 379
NlaIV GGNNCC 2 cut(s) 45, 721
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 1 cut(s) 492
NotI GCGGCCGC 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 185
PcsI WCGNNNNNNNCGW 3 cut(s) 411, 485, 494
PfeI GAWTC 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 7
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 9 cut(s) 235, 253, 256, 355, 358, 361, 370, 373, 514
PleI GAGTC 6 cut(s) 16, 74, 564, 586, 599, 698
PpsI GAGTC 6 cut(s) 16, 74, 564, 586, 599, 698
PshBI ATTAAT 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 303
PspEI GGTNACC 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 45, 721
PspPI GGNCC 1 cut(s) 719
PspXI VCTCGAGB 1 cut(s) 460
PsuI RGATCY 2 cut(s) 113, 171
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 2 cut(s) 254, 440
RsaI GTAC 2 cut(s) 478, 599
RsaNI GTAC 2 cut(s) 477, 598
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 303
SalI GTCGAC 1 cut(s) 517
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 267, 639
SatI GCNGC 9 cut(s) 234, 252, 255, 354, 357, 360, 369, 372, 513
Sau3AI GATC 4 cut(s) 58, 113, 171, 415
Sau96I GGNCC 1 cut(s) 719
SchI GAGTC 6 cut(s) 17, 74, 565, 587, 599, 698
ScrFI CCNGG 3 cut(s) 122, 123, 296
SduI GDGCHC 2 cut(s) 244, 303
SfaNI GCATC 1 cut(s) 132
Sfr274I CTCGAG 1 cut(s) 460
SgrDI CGTCGACG 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 460
SmaI CCCGGG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 2 cut(s) 643, 712
SsiI CCGC 7 cut(s) 233, 279, 354, 360, 368, 372, 524
SspMI CTAG 3 cut(s) 206, 560, 621
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 3 cut(s) 120, 121, 294
StyI CCWWGG 2 cut(s) 375, 629
TaaI ACNGT 2 cut(s) 427, 498
TaiI ACGT 6 cut(s) 109, 152, 348, 476, 482, 603
TaqI TCGA 4 cut(s) 461, 518, 686, 702
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 643, 712
TauI GCSGC 5 cut(s) 236, 356, 362, 371, 374
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 2 cut(s) 267, 639
Tru9I TTAA 2 cut(s) 267, 639
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 4 cut(s) 251, 254, 356, 512
Tsp45I GTSAC 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 13
TspMI CCCGGG 1 cut(s) 121
Tth111I GACNNNGTC 1 cut(s) 7
VneI GTGCAC 1 cut(s) 240
VspI ATTAAT 1 cut(s) 639
XbaI TCTAGA 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 518
XspI CTAG 3 cut(s) 206, 560, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.