AT2G25820

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003146
Physical Location & Seq
Forward (+)
11014789 .. 11015838
1050 bp
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UTR
Exon/CDS
Intron
AT2G25820.1

Sequence Viewer

Length: 678 bp
ATGGTGGATTCTCATGGCTCCGACACGGAATGTTCCTCCAAGAAGAAAAAGGAGAAAACGAAAGAAAAGGGGGTATATCGTGGGGCTCGCATGAGGAGCTGGGGGAAATGGGTCTCGGAGATTCGGGAGCCCCGTAAGAAATCAAGAATCTGGCTCGGGACTTTCCCCACGGCGGAGATGGCAGCGCGTGCCCATGATGTTGCGGCATTGAGTATCAAAGGAAGTTCCGCAATCCTTAACTTCCCTGAGCTCGCGGATTTTCTGCCAAGACCAGTCTCGCTCAGCCAACAGGATATCCAGGCCGCAGCCGCCGAAGCCGCTCTTATGGATTTCAAAACTGTACCATTCCATCTTCAGGATGACTCAACGCCGTTGCAAACTAGGTGTGATACTGAGAAGATCGAAAAGTGGTCATCCTCATCGTCCTCAGCCTCATCCTCATCCTCATCTTCGTCCTCGTCCTCATCATCTATGCTTTCGGGGGAGCTAGGAGATATTGTGGAGTTGCCGAGTCTTGAAAACAATGTAAAATACGATTGTGCGCTGTATGACTCGTTGGAGGGGCTGGTGTCGATGCCCCCATGGTTAGATGCTACCGAAAATGATTTTAGGTATGGAGATGATTCGGTACTGTTGGACCCATGTCTCAAAGAAAGCTTTTTGTGGAATTATGAGTAA

Protein Analysis

225

Amino Acids

24.86

Weight (kDa)

5.05

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 25 - 74 1.4e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 320
AccII CGCG 2 cut(s) 187, 254
AciI CCGC 7 cut(s) 173, 203, 228, 254, 303, 309, 318
AcuI CTGAAG 1 cut(s) 338
AfaI GTAC 2 cut(s) 342, 630
AfiI CCNNNNNNNGG 2 cut(s) 172, 355
AgsI TTSAA 2 cut(s) 334, 518
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 4 cut(s) 99, 250, 487, 657
AluI AGCT 4 cut(s) 99, 250, 487, 657
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 3 cut(s) 118, 280, 650
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 2 cut(s) 182, 305
AspLEI GCGC 2 cut(s) 187, 544
AspS9I GGNCC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 637
BaeGI GKGCMC 1 cut(s) 193
BanII GRGCYC 3 cut(s) 88, 132, 252
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 194, 317
BccI CCATC 2 cut(s) 172, 357
BceAI ACGGC 2 cut(s) 186, 355
BciT130I CCWGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 118, 280, 650
BfaI CTAG 2 cut(s) 381, 488
BisI GCNGC 6 cut(s) 183, 204, 303, 306, 309, 318
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 6 cut(s) 184, 205, 304, 307, 310, 319
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 1 cut(s) 637
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 3 cut(s) 19, 129, 639
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 2 cut(s) 564, 580
BoxI GACNNNNGTC 1 cut(s) 642
Bpu10I CCTNAGC 2 cut(s) 246, 427
Bpu1102I GCTNAGC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 168, 581
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 355
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 168, 581
BseGI GGATG 4 cut(s) 364, 413, 434, 440
BseLI CCNNNNNNNGG 2 cut(s) 172, 355
BseMII CTCAG 4 cut(s) 237, 295, 384, 441
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 2 cut(s) 194, 317
BseYI CCCAGC 1 cut(s) 99
Bsh1236I CGCG 2 cut(s) 187, 254
BshFI GGCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 155
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 2 cut(s) 172, 355
BsmAI GTCTC 3 cut(s) 118, 280, 650
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 302
Bso31I GGTCTC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 4 cut(s) 88, 132, 193, 252
Bsp143I GATC 1 cut(s) 399
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 581
BspACI CCGC 7 cut(s) 173, 203, 228, 254, 303, 309, 318
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 4 cut(s) 238, 294, 385, 440
BspFNI CGCG 2 cut(s) 187, 254
BspLI GGNNCC 3 cut(s) 19, 129, 639
BspTNI GGTCTC 1 cut(s) 118
BsrBI CCGCTC 1 cut(s) 320
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 168, 581
BssMI GATC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 340, 633
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 88, 189, 252
BstDEI CTNAG 4 cut(s) 246, 281, 393, 427
BstDSI CCRYGG 2 cut(s) 168, 581
BstF5I GGATG 4 cut(s) 364, 413, 434, 440
BstFNI CGCG 2 cut(s) 187, 254
BstHHI GCGC 2 cut(s) 187, 544
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 3 cut(s) 118, 280, 650
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 6 cut(s) 96, 179, 188, 308, 314, 317
BstNI CCWGG 1 cut(s) 299
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 2 cut(s) 187, 254
BstV1I GCAGC 2 cut(s) 194, 317
BsuRI GGCC 1 cut(s) 302
BtgI CCRYGG 2 cut(s) 168, 581
BtsCI GGATG 4 cut(s) 364, 413, 434, 440
Cac8I GCNNGC 3 cut(s) 88, 189, 252
CfoI GCGC 2 cut(s) 187, 544
Cfr13I GGNCC 1 cut(s) 637
Csp6I GTAC 2 cut(s) 341, 629
CviAII CATG 5 cut(s) 14, 91, 194, 582, 642
CviQI GTAC 2 cut(s) 341, 629
DdeI CTNAG 4 cut(s) 246, 281, 393, 427
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 581
Eco24I GRGCYC 3 cut(s) 88, 132, 252
Eco31I GGTCTC 1 cut(s) 118
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 637
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 297
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 581
EcoT38I GRGCYC 3 cut(s) 88, 132, 252
ErhI CCWWGG 1 cut(s) 581
FaeI CATG 5 cut(s) 17, 94, 197, 585, 645
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 1 cut(s) 172
FatI CATG 5 cut(s) 13, 90, 193, 581, 641
Fnu4HI GCNGC 6 cut(s) 183, 204, 303, 306, 309, 318
FokI GGATG 4 cut(s) 371, 400, 421, 427
FriOI GRGCYC 3 cut(s) 88, 132, 252
Fsp4HI GCNGC 6 cut(s) 183, 204, 303, 306, 309, 318
FspBI CTAG 2 cut(s) 381, 488
GlaI GCGC 2 cut(s) 186, 543
GluI GCNGC 6 cut(s) 183, 204, 303, 306, 309, 318
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 302
HhaI GCGC 2 cut(s) 187, 544
Hin1II CATG 5 cut(s) 17, 94, 197, 585, 645
Hin6I GCGC 2 cut(s) 185, 542
HinP1I GCGC 2 cut(s) 185, 542
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 7 cut(s) 8, 121, 147, 362, 511, 551, 623
Hpy188I TCNGA 2 cut(s) 22, 118
Hpy188III TCNNGA 5 cut(s) 125, 144, 157, 356, 515
HpyCH4III ACNGT 2 cut(s) 340, 633
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 179, 188, 308, 314, 317
HpyF3I CTNAG 4 cut(s) 246, 281, 393, 427
Hsp92II CATG 5 cut(s) 17, 94, 197, 585, 645
HspAI GCGC 2 cut(s) 185, 542
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 4 cut(s) 23, 96, 127, 484
LpnPI CCDG 9 cut(s) 85, 136, 258, 275, 284, 285, 311, 341, 551
Lsp1109I GCAGC 2 cut(s) 194, 317
LweI GCATC 2 cut(s) 564, 580
MaeI CTAG 2 cut(s) 381, 488
MalI GATC 1 cut(s) 401
MbiI CCGCTC 1 cut(s) 320
MboI GATC 1 cut(s) 399
MboII GAAGA 4 cut(s) 55, 344, 409, 441
MhlI GDGCHC 4 cut(s) 88, 132, 193, 252
MluCI AATT 1 cut(s) 667
MlyI GAGTC 3 cut(s) 356, 520, 545
MmeI TCCRAC 3 cut(s) 45, 537, 615
MseI TTAA 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 2 cut(s) 187, 254
MwoI GCNNNNNNNGC 6 cut(s) 96, 179, 188, 308, 314, 317
NcoI CCATGG 1 cut(s) 581
NdeII GATC 1 cut(s) 399
NlaIII CATG 5 cut(s) 17, 94, 197, 585, 645
NlaIV GGNNCC 3 cut(s) 19, 129, 639
NmeAIII GCCGAG 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PfeI GAWTC 4 cut(s) 8, 121, 147, 623
PkrI GCNGC 6 cut(s) 184, 205, 304, 307, 310, 319
PleI GAGTC 3 cut(s) 356, 519, 545
PpsI GAGTC 3 cut(s) 356, 519, 545
PshAI GACNNNNGTC 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 19, 129, 639
PspPI GGNCC 1 cut(s) 637
RsaI GTAC 2 cut(s) 342, 630
RsaNI GTAC 2 cut(s) 341, 629
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 6 cut(s) 183, 204, 303, 306, 309, 318
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 1 cut(s) 637
SchI GAGTC 3 cut(s) 356, 520, 545
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 4 cut(s) 88, 132, 193, 252
SetI ASST 6 cut(s) 101, 252, 386, 489, 614, 659
SfaNI GCATC 2 cut(s) 564, 580
SinI GGWCC 1 cut(s) 637
Sse9I AATT 1 cut(s) 667
SsiI CCGC 7 cut(s) 173, 203, 228, 254, 303, 309, 318
SspMI CTAG 2 cut(s) 381, 488
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 2 cut(s) 340, 633
TaqI TCGA 2 cut(s) 402, 572
TasI AATT 1 cut(s) 667
TauI GCSGC 4 cut(s) 206, 305, 311, 320
TfiI GAWTC 4 cut(s) 8, 121, 147, 623
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseI GCWGC 2 cut(s) 182, 305
TspGWI ACGGA 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XspI CTAG 2 cut(s) 381, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.