AT2G16405

Transducin family protein WD-40 repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002209
Physical Location & Seq
Reverse (-)
7105339 .. 7109089
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16405.1

Sequence Viewer

Length: 1449 bp
ATGGAGGAGAAAAACGGATCATCAGAGGTAGAGATCGGAGAGAAAGACGAAACGAAGAAGCAATCAAAGGATCTTGAGTTCTTAAGCTGTATGATGCAGCCAAAAACAGCTGATTCAGATCCACACTACATCGGAATCCGCCGGATTCTTCTTCATCGGAAAGCTGATTCTGGCGTTATCTCTCGCCGTTATGATTGGAGATGCAATGGAAAAGGATATGTTGCTTATCGTAATTTCATTAGCAAACCTAGAAAATGGGAGAATCTGCGAACGCCTAGTCTCTTAAGTAGTCCAGGAAACAGTGGTCGATGGTTACCTTCTCCAAGTCCTCTCTCGCATCAATTTGAAGCTGAAAGCTTTACTTCTAGTAGAGATTTAAGGAGTGTGAATCAGGTTTCAAGTCGTAGAGCGAGTTTTAGCTCAAGTTTGAGTGATGGTGATAATTCATATCGTCGTGGTGGTGTTGAACATGCTTATTCTTTTGTTGGAATGCATTGCATCTTCGATCAATGCAAATCTTCTGTTACTGTTCTCAAGTTTGGTCATATGAGTTCTGATATACTTGCATATGGAGCATCAGATGGAAGCTTAACAGTTTGTAGTCTCTTAGAAGAGCCTTCTGTCCTCAAGCAGTTGACAGGTCACTCTAAAGATGTCACAGATTTTGACTTCTCGTCAAACAATCAATACATCGCGTCATCATCACTTGATAAGACAATACGAGTATGGGAGTTGTCAAGAGGCGTTTGTATTAGAGTTATATATGGAATCTCTCCACAGTATTGTATCCGTTTCCACCCTGTTAATAACAATTTCCTCTCTGCTGGCAATGCAAACAAGGAACTCACGGTGTTCAATTTCAGCACTGGGAGAATTATTAAAAAACTGTTGTTTGAGGATGAGGTGACATCTATGGACCATGATCACACTGGCCAAATAATTTTCTGTGGAGATGGGCAGGGAACTGTATATTCAGTAAGCATGGATTCGCATACAGGGTCATTATCCCGGTCTCATCGTCATCGTACAAATCACAAATCTCCTGTCACCACTGTGAAGTACAGAAGCTTCTCCCTCCTGGCATCAGGCCCTGTTCTGCTCACATGTACTCAGGACGGAAACTTGAGTTTCTTTAGTGTTGCCCTTCAGATAAAAGGTTATTTGACTTTGCGTTGCTCCCTCAAATTAGCCCCGCGAATACACAGAATCCAAGCATCGTTTTGCCCGCTATTATCACTAGAGAAAGGAGAATACATAGTTGCTGGAAGCGAAGACTCGAATGTCTACTTCTATGATCTAACCAAACCGAAGCACACATGTGTAAACAAGCTACAGGGTCACAGGTTTCCAGTAATGTGTGTTGCGTGGAACCATGGGGAGAACTTGTTGGCTTCGTCTGATTTCTATGGTGTTGTCATTGTATGGAAAAGAGCAAAGACAAGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

482

Amino Acids

53.97

Weight (kDa)

9.02

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 165 - 286 3.5e-17 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 168 - 248 4.5e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 170 - 475 9.4e-34 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 171 - 257 1.3e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 171 - 288 1.3e-11 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 171 - 379 8.7e-13 WDR55
WD40_Prp19 PF24814 173 - 331 2.7e-14 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 173 - 341 2.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 184 - 376 3.3e-21 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 205 - 297 1.2e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 208 - 243 2.1e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 213 - 363 7.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 218 - 329 3.3e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 263 - 385 2.7e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 333 - 480 6.4e-09 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 351 - 477 1.6e-06 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 402 - 479 6.4e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 413 - 476 8.8e-06 WDR3 first beta-propeller domain
Beta-prop_WDR35_TULP_N PF24797 441 - 479 1.8e-06 WDR35/TULP4 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1280
AccI GTMKAC 1 cut(s) 1284
AccII CGCG 2 cut(s) 695, 1195
AciI CCGC 3 cut(s) 139, 1193, 1226
AclWI GGATC 3 cut(s) 25, 78, 113
AcoI YGGCCR 1 cut(s) 931
AcuI CTGAAG 1 cut(s) 1130
AfaI GTAC 3 cut(s) 1027, 1061, 1108
AflII CTTAAG 2 cut(s) 82, 283
AflIII ACRYGT 2 cut(s) 1103, 1316
AgsI TTSAA 4 cut(s) 347, 399, 467, 856
AhdI GACNNNNNGTC 1 cut(s) 673
AjnI CCWGG 2 cut(s) 292, 1077
AleI CACNNNNGTG 2 cut(s) 1052, 1317
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
Alw26I GTCTC 3 cut(s) 284, 608, 1017
AlwI GGATC 3 cut(s) 25, 78, 113
AlwNI CAGNNNCTG 1 cut(s) 1091
AoxI GGCC 2 cut(s) 931, 1087
ApeKI GCWGC 1 cut(s) 97
ArsI GACNNNNNNTTYG 4 cut(s) 262, 294, 1029, 1061
AspS9I GGNCC 2 cut(s) 916, 1088
AsuC2I CCSGG 1 cut(s) 1009
AsuHPI GGTGA 3 cut(s) 449, 916, 1039
AvaII GGWCC 1 cut(s) 916
BalI TGGCCA 1 cut(s) 933
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BbsI GAAGAC 1 cut(s) 1278
BbvI GCAGC 1 cut(s) 109
BccI CCATC 4 cut(s) 303, 428, 575, 947
BceAI ACGGC 1 cut(s) 171
BciT130I CCWGG 2 cut(s) 294, 1079
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 284, 608, 1017
BfaI CTAG 4 cut(s) 249, 276, 366, 1238
BfmI CTRYAG 1 cut(s) 1331
BfrI CTTAAG 2 cut(s) 82, 283
BfuI GTATCC 1 cut(s) 797
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 3 cut(s) 294, 1009, 1079
Bme18I GGWCC 1 cut(s) 916
BmeRI GACNNNNNGTC 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 916, 1088
BmiI GGNNCC 1 cut(s) 1370
BmrFI CCNGG 3 cut(s) 294, 1009, 1079
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 7 cut(s) 84, 191, 346, 507, 584, 1091, 1223
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 1278
BpuEI CTTGAG 5 cut(s) 95, 406, 518, 611, 1144
BpuMI CCSGG 1 cut(s) 1009
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 1372
BsaXI ACNNNNNCTCC 2 cut(s) 1370, 1400
Bse1I ACTGG 3 cut(s) 871, 934, 1349
Bse3DI GCAATG 3 cut(s) 211, 493, 835
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 2 cut(s) 294, 1079
BseDI CCNNGG 1 cut(s) 1372
BseGI GGATG 1 cut(s) 904
BseJI GATNNNNATC 1 cut(s) 117
BseMI GCAATG 3 cut(s) 211, 493, 835
BseMII CTCAG 1 cut(s) 1124
BseNI ACTGG 3 cut(s) 871, 934, 1349
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 695, 1195
BshFI GGCC 2 cut(s) 933, 1089
BsiSI CCGG 2 cut(s) 142, 1009
BsmAI GTCTC 3 cut(s) 284, 608, 1017
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 2 cut(s) 933, 1089
Bso31I GGTCTC 1 cut(s) 1017
Bsp143I GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
Bsp19I CCATGG 1 cut(s) 1372
BspACI CCGC 3 cut(s) 139, 1193, 1226
BspANI GGCC 2 cut(s) 933, 1089
BspCNI CTCAG 1 cut(s) 1123
BspFNI CGCG 2 cut(s) 695, 1195
BspLI GGNNCC 1 cut(s) 1370
BspPI GGATC 3 cut(s) 25, 78, 113
BspQI GCTCTTC 1 cut(s) 606
BspTI CTTAAG 2 cut(s) 82, 283
BspTNI GGTCTC 1 cut(s) 1017
BsrDI GCAATG 3 cut(s) 211, 493, 835
BsrI ACTGG 3 cut(s) 871, 934, 1349
BssECI CCNNGG 1 cut(s) 1372
BssMI GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
BssT1I CCWWGG 1 cut(s) 1372
Bst2UI CCWGG 2 cut(s) 294, 1079
Bst4CI ACNGT 8 cut(s) 302, 529, 595, 780, 850, 888, 967, 1054
Bst6I CTCTTC 1 cut(s) 606
BstAFI CTTAAG 2 cut(s) 82, 283
BstC8I GCNNGC 2 cut(s) 826, 1226
BstDEI CTNAG 2 cut(s) 607, 1110
BstDSI CCRYGG 1 cut(s) 1372
BstEII GGTNACC 1 cut(s) 312
BstF5I GGATG 1 cut(s) 904
BstFNI CGCG 2 cut(s) 695, 1195
BstKTI GATC 7 cut(s) 20, 36, 73, 121, 508, 925, 1297
BstMAI GTCTC 3 cut(s) 284, 608, 1017
BstMBI GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
BstMWI GCNNNNNNNGC 2 cut(s) 572, 830
BstNI CCWGG 2 cut(s) 294, 1079
BstNSI RCATGY 3 cut(s) 473, 1107, 1320
BstPI GGTNACC 1 cut(s) 312
BstSCI CCNGG 3 cut(s) 292, 1007, 1077
BstSFI CTRYAG 1 cut(s) 1331
BstUI CGCG 2 cut(s) 695, 1195
BstV1I GCAGC 1 cut(s) 109
BstV2I GAAGAC 1 cut(s) 1278
BstX2I RGATCY 2 cut(s) 70, 118
BstYI RGATCY 2 cut(s) 70, 118
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 2 cut(s) 933, 1089
BtgI CCRYGG 1 cut(s) 1372
BtgZI GCGATG 1 cut(s) 676
BtsCI GGATG 1 cut(s) 904
BtsIMutI CAGTG 4 cut(s) 307, 864, 927, 1050
Cac8I GCNNGC 2 cut(s) 826, 1226
CaiI CAGNNNCTG 1 cut(s) 1091
Cfr13I GGNCC 2 cut(s) 916, 1088
CseI GACGC 1 cut(s) 684
Csp6I GTAC 3 cut(s) 1026, 1060, 1107
CviAII CATG 6 cut(s) 470, 920, 982, 1104, 1317, 1373
CviQI GTAC 3 cut(s) 1026, 1060, 1107
DdeI CTNAG 2 cut(s) 607, 1110
DpnI GATC 7 cut(s) 19, 35, 72, 120, 507, 924, 1296
DpnII GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
DrdI GACNNNNNNGTC 1 cut(s) 1280
DriI GACNNNNNGTC 1 cut(s) 673
DseDI GACNNNNNNGTC 1 cut(s) 1280
EaeI YGGCCR 1 cut(s) 931
Eam1104I CTCTTC 1 cut(s) 606
Eam1105I GACNNNNNGTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 606
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 1372
Eco31I GGTCTC 1 cut(s) 1017
Eco47I GGWCC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 1130
Eco91I GGTNACC 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 1088
EcoO65I GGTNACC 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 292, 1077
EcoT14I CCWWGG 1 cut(s) 1372
EcoT22I ATGCAT 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 1372
FaeI CATG 6 cut(s) 473, 923, 985, 1107, 1320, 1376
FalI AAGNNNNNCTT 2 cut(s) 346, 378
FatI CATG 6 cut(s) 469, 919, 981, 1103, 1316, 1372
FauI CCCGC 2 cut(s) 1200, 1233
FauNDI CATATG 2 cut(s) 546, 568
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 1 cut(s) 1284
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 911
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 4 cut(s) 249, 276, 366, 1238
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 2 cut(s) 933, 1089
HapII CCGG 2 cut(s) 142, 1009
HgaI GACGC 1 cut(s) 684
Hin1II CATG 6 cut(s) 473, 923, 985, 1107, 1320, 1376
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HindIII AAGCTT 3 cut(s) 355, 586, 1066
HpaII CCGG 2 cut(s) 142, 1009
HphI GGTGA 3 cut(s) 449, 916, 1039
Hpy166II GTNNAC 3 cut(s) 636, 1285, 1324
Hpy188I TCNGA 9 cut(s) 25, 38, 118, 134, 159, 556, 580, 1149, 1399
Hpy188III TCNNGA 3 cut(s) 74, 738, 1112
Hpy8I GTNNAC 3 cut(s) 636, 1285, 1324
Hpy99I CGWCG 1 cut(s) 456
HpyAV CCTTC 3 cut(s) 327, 627, 1154
HpyCH4III ACNGT 8 cut(s) 302, 529, 595, 780, 850, 888, 967, 1054
HpyCH4V TGCA 7 cut(s) 97, 204, 493, 498, 513, 566, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 830
HpyF3I CTNAG 2 cut(s) 607, 1110
Hsp92II CATG 6 cut(s) 473, 923, 985, 1107, 1320, 1376
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 2 cut(s) 572, 1181
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 7 cut(s) 84, 191, 346, 507, 584, 1091, 1223
MaeI CTAG 4 cut(s) 249, 276, 366, 1238
MaeIII GTNAC 7 cut(s) 312, 523, 641, 655, 904, 1045, 1337
MalI GATC 7 cut(s) 19, 35, 72, 120, 507, 924, 1296
MboI GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
MboII GAAGA 7 cut(s) 67, 140, 143, 493, 510, 623, 1283
MflI RGATCY 2 cut(s) 70, 118
MlsI TGGCCA 1 cut(s) 933
MluCI AATT 8 cut(s) 232, 341, 442, 811, 856, 873, 939, 1184
MluNI TGGCCA 1 cut(s) 933
MlyI GAGTC 1 cut(s) 1268
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 9 cut(s) 19, 339, 635, 734, 827, 889, 895, 1085, 1190
Mox20I TGGCCA 1 cut(s) 933
Mph1103I ATGCAT 1 cut(s) 495
MscI TGGCCA 1 cut(s) 933
MseI TTAA 6 cut(s) 83, 284, 377, 590, 804, 879
MslI CAYNNNNRTG 2 cut(s) 1052, 1317
Msp20I TGGCCA 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 2 cut(s) 82, 283
MspI CCGG 2 cut(s) 142, 1009
MspR9I CCNGG 3 cut(s) 294, 1009, 1079
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 2 cut(s) 294, 1079
MvnI CGCG 2 cut(s) 695, 1195
MwoI GCNNNNNNNGC 2 cut(s) 572, 830
NciI CCSGG 1 cut(s) 1009
NcoI CCATGG 1 cut(s) 1372
NdeI CATATG 2 cut(s) 546, 568
NdeII GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
NlaIII CATG 6 cut(s) 473, 923, 985, 1107, 1320, 1376
NlaIV GGNNCC 1 cut(s) 1370
NmuCI GTSAC 5 cut(s) 641, 655, 904, 1045, 1337
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 3 cut(s) 473, 1107, 1320
OliI CACNNNNGTG 2 cut(s) 1052, 1317
PciI ACATGT 2 cut(s) 1103, 1316
PciSI GCTCTTC 1 cut(s) 606
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 9 cut(s) 113, 135, 145, 167, 262, 388, 768, 986, 1206
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 1268
PpsI GAGTC 1 cut(s) 1268
PscI ACATGT 2 cut(s) 1103, 1316
Psp6I CCWGG 2 cut(s) 292, 1077
PspEI GGTNACC 1 cut(s) 312
PspGI CCWGG 2 cut(s) 292, 1077
PspN4I GGNNCC 1 cut(s) 1370
PspPI GGNCC 2 cut(s) 916, 1088
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 2 cut(s) 70, 118
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 3 cut(s) 1027, 1061, 1108
RsaNI GTAC 3 cut(s) 1026, 1060, 1107
RseI CAYNNNNRTG 2 cut(s) 1052, 1317
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 6 cut(s) 83, 284, 377, 590, 804, 879
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 7 cut(s) 17, 33, 70, 118, 505, 922, 1294
Sau96I GGNCC 2 cut(s) 916, 1088
SchI GAGTC 1 cut(s) 1268
ScrFI CCNGG 3 cut(s) 294, 1009, 1079
SfaNI GCATC 7 cut(s) 84, 191, 346, 507, 584, 1091, 1223
SfcI CTRYAG 1 cut(s) 1331
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 2 cut(s) 1052, 1317
SmlI CTYRAG 7 cut(s) 74, 82, 283, 421, 533, 626, 1123
SmoI CTYRAG 7 cut(s) 74, 82, 283, 421, 533, 626, 1123
Sse9I AATT 8 cut(s) 232, 341, 442, 811, 856, 873, 939, 1184
SsiI CCGC 3 cut(s) 139, 1193, 1226
SspMI CTAG 4 cut(s) 249, 276, 366, 1238
StyD4I CCNGG 3 cut(s) 292, 1007, 1077
StyI CCWWGG 1 cut(s) 1372
TaaI ACNGT 8 cut(s) 302, 529, 595, 780, 850, 888, 967, 1054
TaqI TCGA 3 cut(s) 307, 504, 1277
TasI AATT 8 cut(s) 232, 341, 442, 811, 856, 873, 939, 1184
TatI WGTACW 2 cut(s) 1059, 1106
TfiI GAWTC 9 cut(s) 113, 135, 145, 167, 262, 388, 768, 986, 1206
Tru1I TTAA 6 cut(s) 83, 284, 377, 590, 804, 879
Tru9I TTAA 6 cut(s) 83, 284, 377, 590, 804, 879
TscAI CASTG 4 cut(s) 307, 871, 934, 1057
TseFI GTSAC 5 cut(s) 641, 655, 904, 1045, 1337
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 5 cut(s) 641, 655, 904, 1045, 1337
TspDTI ATGAA 3 cut(s) 143, 226, 435
TspGWI ACGGA 3 cut(s) 30, 779, 1131
TspRI CASTG 4 cut(s) 307, 871, 934, 1057
Vha464I CTTAAG 2 cut(s) 82, 283
VpaK11BI GGWCC 1 cut(s) 916
XceI RCATGY 3 cut(s) 473, 1107, 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 1284
XspI CTAG 4 cut(s) 249, 276, 366, 1238
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.