Rh6BG148600

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002209
Physical Location & Seq
Forward (+)
23844973 .. 23849869
4897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG148600.1

Sequence Viewer

Length: 1080 bp
ATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGATGTGCATAGTGGCAGTCAACCTTCATCTCGTACAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCAATGGGGTGAACAGCCTGCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGGATGGATTGACTGGTATTACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAATGTAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTAACAGTCAAGTTACTGCTATGGACCATGGTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGCAGGCAGAAATCTCCAGTCACAAATGTGGAGTACCGAAGTTTTTTTCTGTTGGCTCGGGCCCTGTGTTGCTGACATGTACTCAGGATGGAAGTTTGTCTTTCTTCCGTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTATGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAGTGTAAACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGGTCATCGATTTCCTGTTATTGGGGTTGCTTGGAACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTGTATGGAAGAGATCAAAGGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

359

Amino Acids

39.55

Weight (kDa)

7.58

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 59 - 157 1.7e-10 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 64 - 149 8.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 65 - 156 1.6e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 66 - 217 1.6e-16 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 66 - 195 3.8e-08 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 69 - 218 9.8e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 71 - 222 3.6e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 79 - 222 2.8e-16 THOC3 beta-propeller domain
WD40 PF00400 104 - 144 9.1e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 125 - 218 5.9e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 165 - 355 5.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 292 - 356 8.8e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 293 - 355 1.1e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 293 - 354 3e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 293 - 357 1e-08 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1064
AccI GTMKAC 1 cut(s) 537
AciI CCGC 1 cut(s) 130
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 938
AcsI RAATTY 1 cut(s) 1015
AfaI GTAC 7 cut(s) 97, 115, 392, 568, 696, 742, 1045
AfiI CCNNNNNNNGG 1 cut(s) 989
AflIII ACRYGT 2 cut(s) 737, 950
AgsI TTSAA 6 cut(s) 220, 306, 441, 559, 875, 912
AhlI ACTAGT 1 cut(s) 116
AleI CACNNNNGTG 2 cut(s) 484, 687
AluBI AGCT 2 cut(s) 237, 964
AluI AGCT 2 cut(s) 237, 964
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 2 cut(s) 109, 718
AoxI GGCC 3 cut(s) 445, 721, 938
ApaI GGGCCC 1 cut(s) 725
ApoI RAATTY 1 cut(s) 1015
AspLEI GCGC 1 cut(s) 778
AspS9I GGNCC 5 cut(s) 4, 576, 619, 721, 722
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 2 cut(s) 158, 491
AvaI CYCGRG 2 cut(s) 109, 718
AvaII GGWCC 3 cut(s) 4, 576, 619
BaeGI GKGCMC 2 cut(s) 231, 725
BanII GRGCYC 2 cut(s) 239, 725
Bbv12I GWGCWC 1 cut(s) 239
BccI CCATC 5 cut(s) 35, 260, 342, 650, 743
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 1 cut(s) 295
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 1064
BglII AGATCT 1 cut(s) 241
BmcAI AGTACT 2 cut(s) 115, 568
Bme1390I CCNGG 1 cut(s) 845
Bme18I GGWCC 3 cut(s) 4, 576, 619
BmeT110I CYCGRG 2 cut(s) 109, 718
BmgT120I GGNCC 5 cut(s) 4, 576, 619, 721, 722
BmiI GGNNCC 3 cut(s) 271, 723, 1004
BmrFI CCNGG 1 cut(s) 845
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 5 cut(s) 192, 269, 325, 407, 1034
BmuI ACTGGG 1 cut(s) 579
BoxI GACNNNNGTC 1 cut(s) 624
BpmI CTGGAG 1 cut(s) 661
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 845
Bsa29I ATCGAT 1 cut(s) 976
BsaJI CCNNGG 3 cut(s) 204, 622, 1006
Bsc4I CCNNNNNNNGG 1 cut(s) 989
Bse1I ACTGG 5 cut(s) 22, 362, 574, 637, 678
Bse3DI GCAATG 3 cut(s) 178, 393, 538
BseCI ATCGAT 1 cut(s) 976
BseDI CCNNGG 3 cut(s) 204, 622, 1006
BseGI GGATG 3 cut(s) 353, 398, 754
BseLI CCNNNNNNNGG 1 cut(s) 989
BseMI GCAATG 3 cut(s) 178, 393, 538
BseMII CTCAG 2 cut(s) 252, 758
BseNI ACTGG 5 cut(s) 22, 362, 574, 637, 678
BseSI GKGCMC 2 cut(s) 231, 725
BsgI GTGCAG 1 cut(s) 217
BshFI GGCC 3 cut(s) 447, 723, 940
BshVI ATCGAT 1 cut(s) 976
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 2 cut(s) 109, 718
BsiSI CCGG 2 cut(s) 844, 897
BslFI GGGAC 2 cut(s) 58, 673
BslI CCNNNNNNNGG 1 cut(s) 989
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 2 cut(s) 58, 673
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 3 cut(s) 447, 723, 940
BsoBI CYCGRG 2 cut(s) 109, 718
Bsp120I GGGCCC 1 cut(s) 721
Bsp1286I GDGCHC 3 cut(s) 231, 239, 725
Bsp143I GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
Bsp19I CCATGG 2 cut(s) 622, 1006
BspACI CCGC 1 cut(s) 130
BspANI GGCC 3 cut(s) 447, 723, 940
BspCNI CTCAG 2 cut(s) 251, 757
BspDI ATCGAT 1 cut(s) 976
BspLI GGNNCC 3 cut(s) 271, 723, 1004
BspMI ACCTGC 1 cut(s) 1064
BspPI GGATC 1 cut(s) 290
BsrDI GCAATG 3 cut(s) 178, 393, 538
BsrI ACTGG 5 cut(s) 22, 362, 574, 637, 678
BssECI CCNNGG 3 cut(s) 204, 622, 1006
BssMI GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
BssT1I CCWWGG 2 cut(s) 622, 1006
Bst4CI ACNGT 8 cut(s) 214, 280, 289, 421, 486, 553, 602, 1048
Bst6I CTCTTC 2 cut(s) 807, 1055
BstC8I GCNNGC 4 cut(s) 134, 156, 249, 666
BstDEI CTNAG 2 cut(s) 238, 744
BstDSI CCRYGG 2 cut(s) 622, 1006
BstF5I GGATG 3 cut(s) 353, 398, 754
BstHHI GCGC 1 cut(s) 778
BstKTI GATC 6 cut(s) 27, 193, 244, 298, 642, 1067
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNSI RCATGY 2 cut(s) 741, 954
BstPAI GACNNNNGTC 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 843
BstSFI CTRYAG 1 cut(s) 965
BstSLI GKGCMC 2 cut(s) 231, 725
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 976
BsuRI GGCC 3 cut(s) 447, 723, 940
BsuTUI ATCGAT 1 cut(s) 976
BtgI CCRYGG 2 cut(s) 622, 1006
BtsCI GGATG 3 cut(s) 353, 398, 754
BtsIMutI CAGTG 4 cut(s) 15, 200, 285, 630
BveI ACCTGC 1 cut(s) 1064
Cac8I GCNNGC 4 cut(s) 134, 156, 249, 666
CfoI GCGC 1 cut(s) 778
Cfr13I GGNCC 5 cut(s) 4, 576, 619, 721, 722
ClaI ATCGAT 1 cut(s) 976
CseI GACGC 2 cut(s) 125, 670
Csp6I GTAC 7 cut(s) 96, 114, 391, 567, 695, 741, 1044
CviAII CATG 6 cut(s) 8, 232, 623, 738, 951, 1007
CviQI GTAC 7 cut(s) 96, 114, 391, 567, 695, 741, 1044
DdeI CTNAG 2 cut(s) 238, 744
DpnI GATC 6 cut(s) 26, 192, 243, 297, 641, 1066
DpnII GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
EaeI YGGCCR 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 807, 1055
EarI CTCTTC 2 cut(s) 807, 1055
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 2 cut(s) 622, 1006
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 239, 725
Eco47I GGWCC 3 cut(s) 4, 576, 619
Eco53kI GAGCTC 1 cut(s) 237
Eco88I CYCGRG 2 cut(s) 109, 718
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 722
EcoT14I CCWWGG 2 cut(s) 622, 1006
EcoT22I ATGCAT 2 cut(s) 180, 455
EcoT38I GRGCYC 2 cut(s) 239, 725
ErhI CCWWGG 2 cut(s) 622, 1006
FaeI CATG 6 cut(s) 11, 235, 626, 741, 954, 1010
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FaqI GGGAC 2 cut(s) 58, 673
FatI CATG 6 cut(s) 7, 231, 622, 737, 950, 1006
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 537
FokI GGATG 3 cut(s) 360, 385, 761
FriOI GRGCYC 2 cut(s) 239, 725
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 777
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 3 cut(s) 447, 723, 940
HapII CCGG 2 cut(s) 844, 897
HgaI GACGC 2 cut(s) 125, 670
HhaI GCGC 1 cut(s) 778
Hin1II CATG 6 cut(s) 11, 235, 626, 741, 954, 1010
Hin6I GCGC 1 cut(s) 776
HinP1I GCGC 1 cut(s) 776
HincII GTYRAC 1 cut(s) 83
HindII GTYRAC 1 cut(s) 83
HinfI GANTC 2 cut(s) 799, 908
HpaII CCGG 2 cut(s) 844, 897
HphI GGTGA 2 cut(s) 158, 491
Hpy166II GTNNAC 5 cut(s) 83, 149, 538, 919, 958
Hpy188I TCNGA 6 cut(s) 64, 241, 265, 295, 321, 1033
Hpy188III TCNNGA 1 cut(s) 746
Hpy8I GTNNAC 5 cut(s) 83, 149, 538, 919, 958
HpyAV CCTTC 2 cut(s) 96, 312
HpyCH4III ACNGT 8 cut(s) 214, 280, 289, 421, 486, 553, 602, 1048
HpyCH4V TGCA 8 cut(s) 70, 158, 178, 183, 198, 285, 398, 453
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 2 cut(s) 238, 744
Hsp92II CATG 6 cut(s) 11, 235, 626, 741, 954, 1010
HspAI GCGC 1 cut(s) 776
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
LmnI GCTCC 1 cut(s) 257
LweI GCATC 5 cut(s) 192, 269, 325, 407, 1034
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 6 cut(s) 208, 326, 340, 607, 626, 680
MalI GATC 6 cut(s) 26, 192, 243, 297, 641, 1066
MboI GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
MboII GAAGA 4 cut(s) 757, 794, 917, 1072
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 3 cut(s) 231, 239, 725
MluCI AATT 5 cut(s) 306, 546, 559, 818, 1015
MlyI GAGTC 1 cut(s) 793
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 4 cut(s) 214, 309, 412, 567
Mph1103I ATGCAT 2 cut(s) 180, 455
MseI TTAA 4 cut(s) 597, 821, 932, 1078
MslI CAYNNNNRTG 4 cut(s) 321, 484, 687, 951
MspI CCGG 2 cut(s) 844, 897
MspR9I CCNGG 1 cut(s) 845
Mva1269I GAATGC 1 cut(s) 180
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 1 cut(s) 845
NcoI CCATGG 2 cut(s) 622, 1006
NdeII GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
NlaIII CATG 6 cut(s) 11, 235, 626, 741, 954, 1010
NlaIV GGNNCC 3 cut(s) 271, 723, 1004
NmeAIII GCCGAG 1 cut(s) 916
NmuCI GTSAC 3 cut(s) 326, 626, 680
NsiI ATGCAT 2 cut(s) 180, 455
NspI RCATGY 2 cut(s) 741, 954
OliI CACNNNNGTG 2 cut(s) 484, 687
PaeR7I CTCGAG 1 cut(s) 109
PceI AGGCCT 1 cut(s) 447
PciI ACATGT 2 cut(s) 737, 950
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 908
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
PscI ACATGT 2 cut(s) 737, 950
PshAI GACNNNNGTC 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 271, 723, 1004
PspOMI GGGCCC 1 cut(s) 721
PspPI GGNCC 5 cut(s) 4, 576, 619, 721, 722
PspXI VCTCGAGB 1 cut(s) 109
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 7 cut(s) 97, 115, 392, 568, 696, 742, 1045
RsaNI GTAC 7 cut(s) 96, 114, 391, 567, 695, 741, 1044
RseI CAYNNNNRTG 4 cut(s) 321, 484, 687, 951
SacI GAGCTC 1 cut(s) 239
SaqAI TTAA 4 cut(s) 597, 821, 932, 1078
Sau3AI GATC 6 cut(s) 24, 190, 241, 295, 639, 1064
Sau96I GGNCC 5 cut(s) 4, 576, 619, 721, 722
ScaI AGTACT 2 cut(s) 115, 568
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 1 cut(s) 845
SduI GDGCHC 3 cut(s) 231, 239, 725
SetI ASST 6 cut(s) 88, 239, 275, 793, 966, 1078
SfaNI GCATC 5 cut(s) 192, 269, 325, 407, 1034
SfcI CTRYAG 1 cut(s) 965
Sfr274I CTCGAG 1 cut(s) 109
SinI GGWCC 3 cut(s) 4, 576, 619
SlaI CTCGAG 1 cut(s) 109
SmiMI CAYNNNNRTG 4 cut(s) 321, 484, 687, 951
SmlI CTYRAG 2 cut(s) 109, 311
SmoI CTYRAG 2 cut(s) 109, 311
SpeI ACTAGT 1 cut(s) 116
Sse9I AATT 5 cut(s) 306, 546, 559, 818, 1015
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 130
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 239
StuI AGGCCT 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 843
StyI CCWWGG 2 cut(s) 622, 1006
TaaI ACNGT 8 cut(s) 214, 280, 289, 421, 486, 553, 602, 1048
TaqI TCGA 2 cut(s) 110, 976
TasI AATT 5 cut(s) 306, 546, 559, 818, 1015
TatI WGTACW 4 cut(s) 113, 390, 566, 740
TfiI GAWTC 1 cut(s) 908
Tru1I TTAA 4 cut(s) 597, 821, 932, 1078
Tru9I TTAA 4 cut(s) 597, 821, 932, 1078
TscAI CASTG 4 cut(s) 22, 200, 292, 637
TseFI GTSAC 3 cut(s) 326, 626, 680
Tsp45I GTSAC 3 cut(s) 326, 626, 680
TspDTI ATGAA 2 cut(s) 78, 572
TspGWI ACGGA 1 cut(s) 758
TspRI CASTG 4 cut(s) 22, 200, 292, 637
VpaK11BI GGWCC 3 cut(s) 4, 576, 619
XapI RAATTY 1 cut(s) 1015
XceI RCATGY 2 cut(s) 741, 954
XcmI CCANNNNNNNNNTGG 2 cut(s) 14, 629
XhoI CTCGAG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 1 cut(s) 117
ZrmI AGTACT 2 cut(s) 115, 568
Zsp2I ATGCAT 2 cut(s) 180, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.