Rh6BG149000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002209
Physical Location & Seq
Forward (+)
23961316 .. 23987132
25817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG149000.1

Sequence Viewer

Length: 738 bp
ATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGAGTATTATCCCGCTCTCATCGGCATTGAAGTAGCAGCAGGCAGAAGCCACCTGATGATGTGCATAGTGGCAGTCAACGTTCATCTCGTACAAGTTTTGGCTCGAGTACCAGTGATAATGACCGCCTGCGTCAATGGGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAAATAACAACTTCCTTTCAGTTGGCAATGTAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTAACAGTCAAGTTACTGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCTCACAGGAGTATTATCCCGTTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAAATGTGGAGTACCGAATTGCCGGAAGTGAAGATTCAAGTGTAAACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

245

Amino Acids

26.72

Weight (kDa)

7.18

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 93 - 236 6.4e-09 WDR5 beta-propeller domain
WD40 PF00400 94 - 119 4.5e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 126 - 234 2.8e-09 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 126 - 234 2.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 417
AciI CCGC 2 cut(s) 60, 171
AclI AACGTT 1 cut(s) 126
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 1 cut(s) 568
AfaI GTAC 5 cut(s) 138, 156, 317, 448, 650
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 5 cut(s) 77, 270, 366, 439, 675
AleI CACNNNNGTG 1 cut(s) 641
AluBI AGCT 2 cut(s) 201, 727
AluI AGCT 2 cut(s) 201, 727
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 2 cut(s) 370, 701
ApeKI GCWGC 2 cut(s) 83, 615
ApoI RAATTY 1 cut(s) 568
AspS9I GGNCC 3 cut(s) 4, 456, 499
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 3 cut(s) 4, 456, 499
BaeGI GKGCMC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 2 cut(s) 95, 627
BccI CCATC 3 cut(s) 35, 224, 530
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BcoDI GTCTC 1 cut(s) 259
BfmI CTRYAG 1 cut(s) 728
BglII AGATCT 1 cut(s) 205
BisI GCNGC 2 cut(s) 84, 616
BlsI GCNGC 2 cut(s) 85, 617
BmcAI AGTACT 1 cut(s) 448
Bme18I GGWCC 3 cut(s) 4, 456, 499
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 4, 456, 499
BmiI GGNNCC 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 4 cut(s) 233, 289, 332, 613
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 260
Bse1I ACTGG 5 cut(s) 22, 158, 454, 517, 632
Bse3DI GCAATG 2 cut(s) 318, 418
BseGI GGATG 1 cut(s) 323
BseMI GCAATG 2 cut(s) 318, 418
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 5 cut(s) 22, 158, 454, 517, 632
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 2 cut(s) 95, 627
BshFI GGCC 2 cut(s) 372, 703
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 2 cut(s) 58, 553
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 2 cut(s) 58, 553
BsnI GGCC 2 cut(s) 372, 703
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 195, 203
Bsp143I GATC 4 cut(s) 24, 205, 259, 519
BspACI CCGC 2 cut(s) 60, 171
BspANI GGCC 2 cut(s) 372, 703
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 2 cut(s) 318, 418
BsrI ACTGG 5 cut(s) 22, 158, 454, 517, 632
BssMI GATC 4 cut(s) 24, 205, 259, 519
Bst4CI ACNGT 5 cut(s) 244, 253, 346, 433, 482
BstC8I GCNNGC 4 cut(s) 88, 175, 213, 620
BstDEI CTNAG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 323
BstKTI GATC 4 cut(s) 27, 208, 262, 522
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 4 cut(s) 24, 205, 259, 519
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 717
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 95, 627
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 2 cut(s) 372, 703
BtsCI GGATG 1 cut(s) 323
BtsIMutI CAGTG 4 cut(s) 15, 165, 249, 510
Cac8I GCNNGC 4 cut(s) 88, 175, 213, 620
Cfr13I GGNCC 3 cut(s) 4, 456, 499
CseI GACGC 1 cut(s) 166
Csp6I GTAC 5 cut(s) 137, 155, 316, 447, 649
CviAII CATG 4 cut(s) 8, 196, 503, 714
CviJI RGCY 6 cut(s) 96, 149, 201, 372, 703, 727
CviKI_1 RGCY 6 cut(s) 96, 149, 201, 372, 703, 727
CviQI GTAC 5 cut(s) 137, 155, 316, 447, 649
DdeI CTNAG 1 cut(s) 202
DpnI GATC 4 cut(s) 26, 207, 261, 521
DpnII GATC 4 cut(s) 24, 205, 259, 519
EaeI YGGCCR 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 201
Eco147I AGGCCT 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 3 cut(s) 4, 456, 499
Eco53kI GAGCTC 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 568
EcoT22I ATGCAT 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 203
FaeI CATG 4 cut(s) 11, 199, 506, 717
FaqI GGGAC 2 cut(s) 58, 553
FatI CATG 4 cut(s) 7, 195, 502, 713
FauI CCCGC 1 cut(s) 67
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 2 cut(s) 84, 616
FokI GGATG 1 cut(s) 310
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 84, 616
GluI GCNGC 2 cut(s) 84, 616
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 2 cut(s) 372, 703
HapII CCGG 1 cut(s) 660
HgaI GACGC 1 cut(s) 166
Hin1II CATG 4 cut(s) 11, 199, 506, 717
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 671
HpaII CCGG 1 cut(s) 660
Hpy166II GTNNAC 4 cut(s) 124, 418, 682, 721
Hpy188I TCNGA 4 cut(s) 205, 229, 259, 285
Hpy8I GTNNAC 4 cut(s) 124, 418, 682, 721
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 5 cut(s) 244, 253, 346, 433, 482
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 4 cut(s) 111, 249, 323, 378
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 1 cut(s) 202
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 4 cut(s) 11, 199, 506, 717
Kzo9I GATC 4 cut(s) 24, 205, 259, 519
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 2 cut(s) 95, 627
LweI GCATC 4 cut(s) 233, 289, 332, 613
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 487, 634
MalI GATC 4 cut(s) 26, 207, 261, 521
MbiI CCGCTC 1 cut(s) 62
MboI GATC 4 cut(s) 24, 205, 259, 519
MboII GAAGA 1 cut(s) 680
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 195, 203
MluCI AATT 5 cut(s) 270, 426, 439, 568, 654
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 3 cut(s) 273, 337, 447
Mph1103I ATGCAT 1 cut(s) 380
MseI TTAA 3 cut(s) 477, 695, 736
MslI CAYNNNNRTG 3 cut(s) 285, 641, 714
MspI CCGG 1 cut(s) 660
MwoI GCNNNNNNNGC 1 cut(s) 221
NdeII GATC 4 cut(s) 24, 205, 259, 519
NlaIII CATG 4 cut(s) 11, 199, 506, 717
NlaIV GGNNCC 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 679
NmuCI GTSAC 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 380
NspI RCATGY 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 150
PceI AGGCCT 1 cut(s) 372
PciI ACATGT 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 1 cut(s) 671
PkrI GCNGC 2 cut(s) 85, 617
PscI ACATGT 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 203
Psp1406I AACGTT 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 3 cut(s) 4, 456, 499
PspXI VCTCGAGB 1 cut(s) 150
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 5 cut(s) 138, 156, 317, 448, 650
RsaNI GTAC 5 cut(s) 137, 155, 316, 447, 649
RseI CAYNNNNRTG 3 cut(s) 285, 641, 714
SacI GAGCTC 1 cut(s) 203
SaqAI TTAA 3 cut(s) 477, 695, 736
SatI GCNGC 2 cut(s) 84, 616
Sau3AI GATC 4 cut(s) 24, 205, 259, 519
Sau96I GGNCC 3 cut(s) 4, 456, 499
ScaI AGTACT 1 cut(s) 448
SduI GDGCHC 2 cut(s) 195, 203
SetI ASST 6 cut(s) 102, 129, 203, 239, 729, 735
SfaNI GCATC 4 cut(s) 233, 289, 332, 613
SfcI CTRYAG 1 cut(s) 728
Sfr274I CTCGAG 1 cut(s) 150
SinI GGWCC 3 cut(s) 4, 456, 499
SlaI CTCGAG 1 cut(s) 150
SmiMI CAYNNNNRTG 3 cut(s) 285, 641, 714
SmlI CTYRAG 2 cut(s) 150, 275
SmoI CTYRAG 2 cut(s) 150, 275
Sse9I AATT 5 cut(s) 270, 426, 439, 568, 654
SseBI AGGCCT 1 cut(s) 372
SsiI CCGC 2 cut(s) 60, 171
SstI GAGCTC 1 cut(s) 203
StuI AGGCCT 1 cut(s) 372
TaaI ACNGT 5 cut(s) 244, 253, 346, 433, 482
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 151, 607
TasI AATT 5 cut(s) 270, 426, 439, 568, 654
TatI WGTACW 2 cut(s) 315, 446
TfiI GAWTC 1 cut(s) 671
Tru1I TTAA 3 cut(s) 477, 695, 736
Tru9I TTAA 3 cut(s) 477, 695, 736
TscAI CASTG 4 cut(s) 22, 165, 256, 517
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 2 cut(s) 83, 615
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 3 cut(s) 119, 452, 581
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 4 cut(s) 22, 165, 256, 517
VpaK11BI GGWCC 3 cut(s) 4, 456, 499
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 717
XcmI CCANNNNNNNNNTGG 2 cut(s) 14, 509
XhoI CTCGAG 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 417
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.