Rw6G012840

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002209
Physical Location & Seq
Forward (+)
23668246 .. 23678922
10677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G012840.1

Sequence Viewer

Length: 1155 bp
ATGTCTGCCTCCCAGAAGACAGTATGCGAATTCTCCAACTTCTTTTGTCCCTCGGGAGAACAACCTTCACAAAAAAAGTGGTTAAGTCTTTCAGCTTCCTCTGCACTCATTGGGCGATGGATTCAACCTCCGAGTCCACTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGTGCATAGTGGCAGTCAACCTTCATCTCGTACAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCAACGGGGTGAACAGCCTTCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCGTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTTCATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAACGTAGACAAAGAAATTACGTTACTGCTATATATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGTTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTTTTTCTGTTGGCTCGGGCCCTTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTATGCTTTCCCTTGAGAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAGTGTATACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGTTTGGTCTTCTGTTATTGGGGTTGCTTGGCACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTGTATGGAAGAGATCAAAGGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

384

Amino Acids

42.6

Weight (kDa)

8.83

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 99 - 217 1.5e-15 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 103 - 182 1.8e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 104 - 191 6.2e-12 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 105 - 379 2.5e-23 WDR5 beta-propeller domain
WD40_Prp19 PF24814 108 - 211 5.8e-12 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 108 - 224 1.1e-12 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 108 - 210 8.8e-06 EIF3I
WD40_CDC20-Fz PF24807 111 - 260 7.4e-09 CDC20/Fizzy WD40 domain
WDR55 PF24796 116 - 180 6.8e-07 WDR55
Beta-prop_THOC3 PF25174 118 - 219 4e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 125 - 215 2.8e-06 WDR3 second beta-propeller domain
WD40 PF00400 142 - 177 2.9e-10 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 142 - 188 1.1e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 1139
AccB1I GGYRCC 1 cut(s) 1077
AccI GTMKAC 2 cut(s) 636, 996
AciI CCGC 1 cut(s) 247
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 1 cut(s) 1016
AcsI RAATTY 3 cut(s) 29, 736, 1090
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 4 cut(s) 214, 232, 818, 1120
AflIII ACRYGT 1 cut(s) 1028
AgsI TTSAA 5 cut(s) 125, 337, 423, 540, 990
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 1 cut(s) 172
AleI CACNNNNGTG 3 cut(s) 148, 583, 809
AluBI AGCT 4 cut(s) 95, 165, 354, 1042
AluI AGCT 4 cut(s) 95, 165, 354, 1042
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 3 cut(s) 52, 226, 840
AoxI GGCC 3 cut(s) 544, 843, 1016
ApaI GGGCCC 1 cut(s) 847
ApeKI GCWGC 1 cut(s) 783
ApoI RAATTY 3 cut(s) 29, 736, 1090
AspLEI GCGC 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 667, 843, 844
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 2 cut(s) 275, 590
AvaI CYCGRG 3 cut(s) 52, 226, 840
AvaII GGWCC 1 cut(s) 667
BaeGI GKGCMC 2 cut(s) 348, 847
BanI GGYRCC 1 cut(s) 1077
BanII GRGCYC 2 cut(s) 356, 847
BbsI GAAGAC 2 cut(s) 23, 1047
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 1 cut(s) 795
BccI CCATC 3 cut(s) 111, 377, 698
BcgI CGANNNNNNTGC 2 cut(s) 486, 520
BciT130I CCWGG 1 cut(s) 174
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 1 cut(s) 412
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 1043
BfuAI ACCTGC 1 cut(s) 1139
BglII AGATCT 1 cut(s) 358
BisI GCNGC 1 cut(s) 784
BlsI GCNGC 1 cut(s) 785
BmcAI AGTACT 1 cut(s) 232
Bme1390I CCNGG 2 cut(s) 174, 923
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 3 cut(s) 52, 226, 840
BmgT120I GGNCC 3 cut(s) 667, 843, 844
BmiI GGNNCC 3 cut(s) 388, 845, 1079
BmrFI CCNGG 2 cut(s) 174, 923
BmsI GCATC 6 cut(s) 309, 386, 442, 506, 781, 1109
BpiI GAAGAC 2 cut(s) 23, 1047
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 1 cut(s) 783
BpuEI CTTGAG 2 cut(s) 413, 971
BpuMI CCSGG 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 51, 321, 1081
Bse1I ACTGG 2 cut(s) 685, 800
Bse3DI GCAATG 2 cut(s) 295, 492
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 51, 321, 1081
BseGI GGATG 2 cut(s) 187, 497
BseMI GCAATG 2 cut(s) 295, 492
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 685, 800
BseSI GKGCMC 2 cut(s) 348, 847
BseXI GCAGC 1 cut(s) 795
BsgI GTGCAG 2 cut(s) 87, 334
BshFI GGCC 3 cut(s) 546, 845, 1018
BshNI GGYRCC 1 cut(s) 1077
BsiHKAI GWGCWC 1 cut(s) 356
BsiHKCI CYCGRG 3 cut(s) 52, 226, 840
BsiSI CCGG 2 cut(s) 922, 975
BslFI GGGAC 2 cut(s) 33, 721
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 2 cut(s) 33, 721
BsmI GAATGC 1 cut(s) 297
BsnI GGCC 3 cut(s) 546, 845, 1018
BsoBI CYCGRG 3 cut(s) 52, 226, 840
Bsp120I GGGCCC 1 cut(s) 843
Bsp1286I GDGCHC 3 cut(s) 348, 356, 847
Bsp143I GATC 5 cut(s) 307, 358, 412, 687, 1139
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 1 cut(s) 247
BspANI GGCC 3 cut(s) 546, 845, 1018
BspCNI CTCAG 1 cut(s) 368
BspLI GGNNCC 3 cut(s) 388, 845, 1079
BspMI ACCTGC 1 cut(s) 1139
BspPI GGATC 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 1077
BsrDI GCAATG 2 cut(s) 295, 492
BsrI ACTGG 2 cut(s) 685, 800
BssECI CCNNGG 3 cut(s) 51, 321, 1081
BssMI GATC 5 cut(s) 307, 358, 412, 687, 1139
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 1081
Bst1107I GTATAC 1 cut(s) 997
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 8 cut(s) 22, 331, 397, 406, 520, 585, 811, 1123
Bst6I CTCTTC 2 cut(s) 885, 1130
BstACI GRCGYC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 251, 366, 788
BstDEI CTNAG 1 cut(s) 355
BstDSI CCRYGG 1 cut(s) 1081
BstF5I GGATG 2 cut(s) 187, 497
BstHHI GCGC 1 cut(s) 856
BstKTI GATC 5 cut(s) 310, 361, 415, 690, 1142
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 5 cut(s) 307, 358, 412, 687, 1139
BstMWI GCNNNNNNNGC 3 cut(s) 101, 374, 851
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 1032
BstSCI CCNGG 2 cut(s) 172, 921
BstSFI CTRYAG 1 cut(s) 1043
BstSLI GKGCMC 2 cut(s) 348, 847
BstV1I GCAGC 1 cut(s) 795
BstV2I GAAGAC 2 cut(s) 23, 1047
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BstZ17I GTATAC 1 cut(s) 997
BsuRI GGCC 3 cut(s) 546, 845, 1018
BtgI CCRYGG 1 cut(s) 1081
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 2 cut(s) 187, 497
BtsIMutI CAGTG 3 cut(s) 317, 402, 678
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 3 cut(s) 251, 366, 788
CfoI GCGC 1 cut(s) 856
Cfr13I GGNCC 3 cut(s) 667, 843, 844
CseI GACGC 1 cut(s) 242
Csp6I GTAC 4 cut(s) 213, 231, 817, 1119
CspCI CAANNNNNGTGG 2 cut(s) 59, 94
CviAII CATG 4 cut(s) 349, 671, 1029, 1082
CviQI GTAC 4 cut(s) 213, 231, 817, 1119
DdeI CTNAG 1 cut(s) 355
DpnI GATC 5 cut(s) 309, 360, 414, 689, 1141
DpnII GATC 5 cut(s) 307, 358, 412, 687, 1139
EaeI YGGCCR 1 cut(s) 1016
Eam1104I CTCTTC 2 cut(s) 885, 1130
EarI CTCTTC 2 cut(s) 885, 1130
Ecl136II GAGCTC 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 1081
Eco147I AGGCCT 1 cut(s) 546
Eco24I GRGCYC 2 cut(s) 356, 847
Eco47I GGWCC 1 cut(s) 667
Eco53kI GAGCTC 1 cut(s) 354
Eco88I CYCGRG 3 cut(s) 52, 226, 840
EcoICRI GAGCTC 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 844
EcoRI GAATTC 2 cut(s) 29, 736
EcoRII CCWGG 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 1081
EcoT22I ATGCAT 2 cut(s) 297, 554
EcoT38I GRGCYC 2 cut(s) 356, 847
ErhI CCWWGG 1 cut(s) 1081
FaeI CATG 4 cut(s) 352, 674, 1032, 1085
FaqI GGGAC 2 cut(s) 33, 721
FatI CATG 4 cut(s) 348, 670, 1028, 1081
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 2 cut(s) 636, 996
Fnu4HI GCNGC 1 cut(s) 784
FokI GGATG 2 cut(s) 194, 484
FriOI GRGCYC 2 cut(s) 356, 847
Fsp4HI GCNGC 1 cut(s) 784
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 855
GluI GCNGC 1 cut(s) 784
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 3 cut(s) 546, 845, 1018
HapII CCGG 2 cut(s) 922, 975
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 856
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 4 cut(s) 352, 674, 1032, 1085
Hin6I GCGC 1 cut(s) 854
HinP1I GCGC 1 cut(s) 854
HincII GTYRAC 2 cut(s) 200, 257
HindII GTYRAC 2 cut(s) 200, 257
HinfI GANTC 5 cut(s) 121, 133, 169, 877, 986
HpaII CCGG 2 cut(s) 922, 975
HphI GGTGA 2 cut(s) 275, 590
Hpy166II GTNNAC 7 cut(s) 137, 200, 257, 266, 637, 997, 1036
Hpy188I TCNGA 6 cut(s) 132, 358, 382, 412, 438, 1108
Hpy188III TCNNGA 2 cut(s) 54, 160
Hpy8I GTNNAC 7 cut(s) 137, 200, 257, 266, 637, 997, 1036
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 75, 213, 282
HpyCH4III ACNGT 8 cut(s) 22, 331, 397, 406, 520, 585, 811, 1123
HpyCH4IV ACGT 3 cut(s) 156, 633, 650
HpyCH4V TGCA 8 cut(s) 104, 187, 295, 300, 315, 402, 497, 552
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 374, 851
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 3 cut(s) 156, 633, 650
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 4 cut(s) 352, 674, 1032, 1085
HspAI GCGC 1 cut(s) 854
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 5 cut(s) 307, 358, 412, 687, 1139
LmnI GCTCC 1 cut(s) 374
Lsp1109I GCAGC 1 cut(s) 795
LweI GCATC 6 cut(s) 309, 386, 442, 506, 781, 1109
MaeI CTAG 1 cut(s) 234
MaeII ACGT 3 cut(s) 156, 633, 650
MaeIII GTNAC 6 cut(s) 152, 325, 443, 457, 651, 802
MalI GATC 5 cut(s) 309, 360, 414, 689, 1141
MboI GATC 5 cut(s) 307, 358, 412, 687, 1139
MboII GAAGA 5 cut(s) 28, 872, 995, 1047, 1147
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 3 cut(s) 348, 356, 847
MluCI AATT 6 cut(s) 29, 423, 645, 736, 896, 1090
MlyI GAGTC 3 cut(s) 142, 163, 871
MmeI TCCRAC 2 cut(s) 60, 489
MnlI CCTC 7 cut(s) 19, 61, 109, 138, 331, 426, 511
Mph1103I ATGCAT 2 cut(s) 297, 554
MseI TTAA 4 cut(s) 83, 899, 1010, 1153
MslI CAYNNNNRTG 5 cut(s) 148, 438, 583, 809, 1029
MspI CCGG 2 cut(s) 922, 975
MspR9I CCNGG 2 cut(s) 174, 923
Mva1269I GAATGC 1 cut(s) 297
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 3 cut(s) 101, 374, 851
NciI CCSGG 1 cut(s) 923
NcoI CCATGG 1 cut(s) 1081
NdeII GATC 5 cut(s) 307, 358, 412, 687, 1139
NlaIII CATG 4 cut(s) 352, 674, 1032, 1085
NlaIV GGNNCC 3 cut(s) 388, 845, 1079
NmeAIII GCCGAG 1 cut(s) 994
NmuCI GTSAC 3 cut(s) 152, 443, 802
NsiI ATGCAT 2 cut(s) 297, 554
NspI RCATGY 1 cut(s) 1032
OliI CACNNNNGTG 3 cut(s) 148, 583, 809
PaeR7I CTCGAG 1 cut(s) 226
PceI AGGCCT 1 cut(s) 546
PciI ACATGT 1 cut(s) 1028
PctI GAATGC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 121, 986
PkrI GCNGC 1 cut(s) 785
PleI GAGTC 3 cut(s) 141, 163, 871
PpsI GAGTC 3 cut(s) 141, 163, 871
PscI ACATGT 1 cut(s) 1028
Psp124BI GAGCTC 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 3 cut(s) 388, 845, 1079
PspOMI GGGCCC 1 cut(s) 843
PspPI GGNCC 3 cut(s) 667, 843, 844
PspXI VCTCGAGB 1 cut(s) 226
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 4 cut(s) 214, 232, 818, 1120
RsaNI GTAC 4 cut(s) 213, 231, 817, 1119
RseI CAYNNNNRTG 5 cut(s) 148, 438, 583, 809, 1029
SacI GAGCTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 83, 899, 1010, 1153
SatI GCNGC 1 cut(s) 784
Sau3AI GATC 5 cut(s) 307, 358, 412, 687, 1139
Sau96I GGNCC 3 cut(s) 667, 843, 844
ScaI AGTACT 1 cut(s) 232
SchI GAGTC 3 cut(s) 142, 163, 871
ScrFI CCNGG 2 cut(s) 174, 923
SduI GDGCHC 3 cut(s) 348, 356, 847
SfaNI GCATC 6 cut(s) 309, 386, 442, 506, 781, 1109
SfcI CTRYAG 1 cut(s) 1043
Sfr274I CTCGAG 1 cut(s) 226
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 226
SmiMI CAYNNNNRTG 5 cut(s) 148, 438, 583, 809, 1029
SmlI CTYRAG 3 cut(s) 226, 428, 950
SmoI CTYRAG 3 cut(s) 226, 428, 950
SpeI ACTAGT 1 cut(s) 233
Sse9I AATT 6 cut(s) 29, 423, 645, 736, 896, 1090
SseBI AGGCCT 1 cut(s) 546
SsiI CCGC 1 cut(s) 247
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 356
StuI AGGCCT 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 172, 921
StyI CCWWGG 1 cut(s) 1081
TaaI ACNGT 8 cut(s) 22, 331, 397, 406, 520, 585, 811, 1123
TaiI ACGT 3 cut(s) 159, 636, 653
TaqI TCGA 3 cut(s) 159, 227, 775
TasI AATT 6 cut(s) 29, 423, 645, 736, 896, 1090
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 2 cut(s) 121, 986
Tru1I TTAA 4 cut(s) 83, 899, 1010, 1153
Tru9I TTAA 4 cut(s) 83, 899, 1010, 1153
TscAI CASTG 3 cut(s) 317, 409, 685
TseFI GTSAC 3 cut(s) 152, 443, 802
TseI GCWGC 1 cut(s) 783
Tsp45I GTSAC 3 cut(s) 152, 443, 802
TspDTI ATGAA 4 cut(s) 195, 264, 481, 749
TspRI CASTG 3 cut(s) 317, 409, 685
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 3 cut(s) 29, 736, 1090
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 226
XmiI GTMKAC 2 cut(s) 636, 996
XspI CTAG 1 cut(s) 234
ZraI GACGTC 1 cut(s) 157
ZrmI AGTACT 1 cut(s) 232
Zsp2I ATGCAT 2 cut(s) 297, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.