RLG00000013952

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002209
Physical Location & Seq
Forward (+)
44692549 .. 44693950
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013952

Sequence Viewer

Length: 351 bp
ATGCAACGTTCATATCTCAAGTTCATATTTCAACCTCCGAGTCCGCTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGTGCATAGTGGCAGTCAACCTTCATCTCGTAGAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCGACAGGATCTGCTTGCATATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATCCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGGTGGATAAAACTGTGAGAGTATGGGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

117

Amino Acids

12.9

Weight (kDa)

6.25

Isoelectric Point (pI)

56.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_2nd PF25168 54 - 116 3.7e-08 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 55 - 115 1.3e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 55 - 115 1.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 55 - 116 2.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 56 - 116 4.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 56 - 116 1.9e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 56 - 115 1e-07 THOC3 beta-propeller domain
WDR55 PF24796 56 - 116 2.4e-06 WDR55
Beta-prop_WDR5 PF25175 57 - 115 2e-09 WDR5 beta-propeller domain
WD40 PF00400 79 - 114 4.8e-11 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 79 - 116 5.3e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 163
AciI CCGC 2 cut(s) 44, 154
AclI AACGTT 1 cut(s) 7
AclWI GGATC 2 cut(s) 177, 218
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 139, 302
AgsI TTSAA 2 cut(s) 32, 234
AhlI ACTAGT 1 cut(s) 140
AjnI CCWGG 1 cut(s) 79
AleI CACNNNNGTG 1 cut(s) 55
AluBI AGCT 1 cut(s) 72
AluI AGCT 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 177, 218
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 133
BccI CCATC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 223
BcuI ACTAGT 1 cut(s) 140
BfaI CTAG 1 cut(s) 141
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 3 cut(s) 197, 253, 317
BplI GAGNNNNNCTC 2 cut(s) 61, 93
BpuEI CTTGAG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 81
BseGI GGATG 2 cut(s) 94, 308
BseMI GCAATG 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 223
BsoBI CYCGRG 1 cut(s) 133
Bsp143I GATC 2 cut(s) 169, 223
BspACI CCGC 2 cut(s) 44, 154
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 2 cut(s) 177, 218
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 303
BssMI GATC 2 cut(s) 169, 223
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 3 cut(s) 208, 217, 331
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 158, 177
BstF5I GGATG 2 cut(s) 94, 308
BstKTI GATC 2 cut(s) 172, 226
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 2 cut(s) 169, 223
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BtsCI GGATG 2 cut(s) 94, 308
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 158, 177
CaiI CAGNNNCTG 1 cut(s) 172
CseI GACGC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 138, 301
CviJI RGCY 2 cut(s) 72, 132
CviKI_1 RGCY 2 cut(s) 72, 132
CviQI GTAC 2 cut(s) 138, 301
DpnI GATC 2 cut(s) 171, 225
DpnII GATC 2 cut(s) 169, 223
Eco88I CYCGRG 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 79
FaiI YATR 7 cut(s) 13, 26, 96, 181, 183, 211, 340
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 163
FokI GGATG 2 cut(s) 101, 295
FspBI CTAG 1 cut(s) 141
HgaI GACGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 63
HincII GTYRAC 2 cut(s) 107, 164
HindII GTYRAC 2 cut(s) 107, 164
HinfI GANTC 2 cut(s) 40, 76
Hpy166II GTNNAC 2 cut(s) 107, 164
Hpy188I TCNGA 4 cut(s) 39, 193, 223, 249
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 2 cut(s) 107, 164
Hpy99I CGWCG 2 cut(s) 68, 165
HpyAV CCTTC 2 cut(s) 120, 240
HpyCH4III ACNGT 3 cut(s) 208, 217, 331
HpyCH4IV ACGT 2 cut(s) 7, 63
HpyCH4V TGCA 5 cut(s) 4, 94, 179, 213, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpySE526I ACGT 2 cut(s) 7, 63
Hsp92I GRCGYC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 169, 223
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 6 cut(s) 58, 66, 70, 93, 152, 170
LweI GCATC 3 cut(s) 197, 253, 317
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 7, 63
MaeIII GTNAC 3 cut(s) 59, 254, 268
MalI GATC 2 cut(s) 171, 225
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 169, 223
MflI RGATCY 1 cut(s) 169
MlyI GAGTC 2 cut(s) 49, 70
MnlI CCTC 4 cut(s) 45, 237, 248, 322
MslI CAYNNNNRTG 2 cut(s) 55, 249
MspR9I CCNGG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 169, 223
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 59, 254
OliI CACNNNNGTG 1 cut(s) 55
PaeR7I CTCGAG 1 cut(s) 133
PleI GAGTC 2 cut(s) 48, 70
PpsI GAGTC 2 cut(s) 48, 70
Psp1406I AACGTT 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 2 cut(s) 139, 302
RsaNI GTAC 2 cut(s) 138, 301
RseI CAYNNNNRTG 2 cut(s) 55, 249
SalI GTCGAC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 169, 223
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 2 cut(s) 49, 70
ScrFI CCNGG 1 cut(s) 81
SetI ASST 6 cut(s) 10, 37, 66, 74, 112, 203
SfaNI GCATC 3 cut(s) 197, 253, 317
Sfr274I CTCGAG 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 55, 249
SmlI CTYRAG 3 cut(s) 17, 133, 239
SmoI CTYRAG 3 cut(s) 17, 133, 239
SpeI ACTAGT 1 cut(s) 140
SsiI CCGC 2 cut(s) 44, 154
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 3 cut(s) 208, 217, 331
TaiI ACGT 2 cut(s) 10, 66
TaqI TCGA 3 cut(s) 66, 134, 163
TatI WGTACW 2 cut(s) 137, 300
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 59, 254
Tsp45I GTSAC 2 cut(s) 59, 254
TspDTI ATGAA 2 cut(s) 13, 102
TspRI CASTG 1 cut(s) 220
XhoI CTCGAG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 1 cut(s) 141
ZraI GACGTC 1 cut(s) 64
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.