Rroxscaffold_7G00195140

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002209
Physical Location & Seq
Forward (+)
37376852 .. 37382098
5247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195140.1

Sequence Viewer

Length: 837 bp
ATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGATCGGATCTGCTTGCGTATGGAGCACCAGATGGAACCTTGATAGTATGCACTGTCTCCGATCCTCCTTCAATTCTCAAGCATTTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAATGCAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACACTCAAGTTACCGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGATGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTTTCTCTGTTGGCTCGGGGCTCTGTGTTGCTGACATGTACTCAAGATGGAAGTTTGTCTTTCTTCAGTGTTGCGCTGGAACTACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTCTGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAATGTCTACTTCTACAACTTAACTCGGCCAAAGCATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

278

Amino Acids

30.88

Weight (kDa)

8.79

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 3 - 93 4.3e-14 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 7 - 85 3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 8 - 273 9.3e-28 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 8 - 161 5.8e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 8 - 218 2.4e-11 WDR55
WD40_WDHD1_1st PF24817 8 - 92 1.3e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 11 - 169 3.5e-15 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 128 7e-12 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 20 - 187 1.1e-15 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 22 - 268 2.4e-22 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 45 - 144 7.8e-10 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 46 - 80 1.7e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 47 - 219 5.7e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 49 - 181 6.3e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 55 - 164 2.2e-08 WDR3 second beta-propeller domain
WDR90_beta-prop_4th PF23342 56 - 218 1.4e-06 WDR90, 4th beta-propeller
Beta-prop_EML_2 PF23414 101 - 224 1.9e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 522
AccI GTMKAC 1 cut(s) 801
AciI CCGC 2 cut(s) 420, 520
AclWI GGATC 2 cut(s) 75, 116
AcoI YGGCCR 1 cut(s) 821
AcsI RAATTY 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 634
AfaI GTAC 4 cut(s) 200, 376, 578, 625
AflIII ACRYGT 1 cut(s) 620
AgsI TTSAA 7 cut(s) 46, 132, 148, 249, 367, 758, 795
AleI CACNNNNGTG 2 cut(s) 292, 569
Alw21I GWGCWC 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 75, 116
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 2 cut(s) 253, 821
ApeKI GCWGC 1 cut(s) 543
ApoI RAATTY 1 cut(s) 496
Asp700I GAANNNNTTC 1 cut(s) 586
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 2 cut(s) 384, 427
AsuC2I CCSGG 1 cut(s) 728
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 2 cut(s) 384, 427
BaeGI GKGCMC 1 cut(s) 57
BanII GRGCYC 1 cut(s) 608
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 555
BccI CCATC 4 cut(s) 86, 458, 463, 626
BcgI CGANNNNNNTGC 2 cut(s) 195, 229
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 728
BcoDI GTCTC 1 cut(s) 121
BisI GCNGC 1 cut(s) 544
BlsI GCNGC 1 cut(s) 545
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 728
Bme18I GGWCC 2 cut(s) 384, 427
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 2 cut(s) 384, 427
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 728
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 3 cut(s) 18, 215, 541
BmuI ACTGGG 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 3 cut(s) 122, 396, 612
BpuMI CCSGG 1 cut(s) 728
BsaJI CCNNGG 1 cut(s) 30
Bse1I ACTGG 3 cut(s) 382, 445, 560
Bse3DI GCAATG 3 cut(s) 4, 201, 346
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 2 cut(s) 206, 474
BseMI GCAATG 3 cut(s) 4, 201, 346
BseNI ACTGG 3 cut(s) 382, 445, 560
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 1 cut(s) 555
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 2 cut(s) 255, 823
BsiHKAI GWGCWC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 2 cut(s) 727, 780
BsmAI GTCTC 1 cut(s) 121
BsnI GGCC 2 cut(s) 255, 823
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 3 cut(s) 57, 88, 608
Bsp143I GATC 5 cut(s) 16, 62, 67, 121, 447
BspACI CCGC 2 cut(s) 420, 520
BspANI GGCC 2 cut(s) 255, 823
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 2 cut(s) 75, 116
BsrBI CCGCTC 1 cut(s) 522
BsrDI GCAATG 3 cut(s) 4, 201, 346
BsrI ACTGG 3 cut(s) 382, 445, 560
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 5 cut(s) 16, 62, 67, 121, 447
Bst4CI ACNGT 6 cut(s) 40, 115, 229, 294, 361, 571
Bst6I CTCTTC 1 cut(s) 690
BstC8I GCNNGC 2 cut(s) 75, 548
BstF5I GGATG 2 cut(s) 206, 474
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 5 cut(s) 19, 65, 70, 124, 450
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 5 cut(s) 16, 62, 67, 121, 447
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 726
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 555
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 2 cut(s) 255, 823
BtsCI GGATG 2 cut(s) 206, 474
BtsIMutI CAGTG 4 cut(s) 26, 111, 438, 658
Cac8I GCNNGC 2 cut(s) 75, 548
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 2 cut(s) 384, 427
Csp6I GTAC 4 cut(s) 199, 375, 577, 624
CviAII CATG 4 cut(s) 58, 431, 621, 834
CviJI RGCY 6 cut(s) 29, 255, 599, 606, 731, 823
CviKI_1 RGCY 6 cut(s) 29, 255, 599, 606, 731, 823
CviQI GTAC 4 cut(s) 199, 375, 577, 624
DpnI GATC 5 cut(s) 18, 64, 69, 123, 449
DpnII GATC 5 cut(s) 16, 62, 67, 121, 447
EaeI YGGCCR 1 cut(s) 821
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
Eco147I AGGCCT 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 384, 427
Eco57I CTGAAG 1 cut(s) 634
Eco88I CYCGRG 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 496
EcoT22I ATGCAT 2 cut(s) 6, 263
EcoT38I GRGCYC 1 cut(s) 608
FaeI CATG 4 cut(s) 61, 434, 624, 837
FalI AAGNNNNNCTT 2 cut(s) 628, 660
FatI CATG 4 cut(s) 57, 430, 620, 833
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 1 cut(s) 544
FokI GGATG 2 cut(s) 193, 481
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 1 cut(s) 544
GlaI GCGC 1 cut(s) 660
GluI GCNGC 1 cut(s) 544
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 2 cut(s) 255, 823
HapII CCGG 2 cut(s) 727, 780
HhaI GCGC 1 cut(s) 661
Hin1II CATG 4 cut(s) 61, 434, 624, 837
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HinfI GANTC 2 cut(s) 682, 791
HpaII CCGG 2 cut(s) 727, 780
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 2 cut(s) 67, 121
Hpy188III TCNNGA 1 cut(s) 629
Hpy8I GTNNAC 1 cut(s) 802
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 6 cut(s) 40, 115, 229, 294, 361, 571
HpyCH4V TGCA 7 cut(s) 4, 9, 24, 111, 206, 261, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
Hsp92II CATG 4 cut(s) 61, 434, 624, 837
HspAI GCGC 1 cut(s) 659
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 62, 67, 121, 447
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 1 cut(s) 555
LweI GCATC 3 cut(s) 18, 215, 541
MaeIII GTNAC 5 cut(s) 34, 152, 166, 415, 562
MalI GATC 5 cut(s) 18, 64, 69, 123, 449
MbiI CCGCTC 1 cut(s) 522
MboI GATC 5 cut(s) 16, 62, 67, 121, 447
MboII GAAGA 3 cut(s) 640, 677, 800
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 3 cut(s) 57, 88, 608
MluCI AATT 5 cut(s) 132, 354, 367, 496, 701
MlyI GAGTC 1 cut(s) 676
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 5 cut(s) 40, 135, 220, 375, 753
Mph1103I ATGCAT 2 cut(s) 6, 263
MroXI GAANNNNTTC 1 cut(s) 586
MseI TTAA 2 cut(s) 704, 815
MslI CAYNNNNRTG 3 cut(s) 147, 292, 569
MspI CCGG 2 cut(s) 727, 780
MspR9I CCNGG 1 cut(s) 728
MwoI GCNNNNNNNGC 1 cut(s) 83
NciI CCSGG 1 cut(s) 728
NdeII GATC 5 cut(s) 16, 62, 67, 121, 447
NlaIII CATG 4 cut(s) 61, 434, 624, 837
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 2 cut(s) 152, 562
NsiI ATGCAT 2 cut(s) 6, 263
NspI RCATGY 1 cut(s) 624
OliI CACNNNNGTG 2 cut(s) 292, 569
PceI AGGCCT 1 cut(s) 255
PciI ACATGT 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 1 cut(s) 791
PkrI GCNGC 1 cut(s) 545
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
PscI ACATGT 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 384, 427
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 4 cut(s) 200, 376, 578, 625
RsaNI GTAC 4 cut(s) 199, 375, 577, 624
RseI CAYNNNNRTG 3 cut(s) 147, 292, 569
SaqAI TTAA 2 cut(s) 704, 815
SatI GCNGC 1 cut(s) 544
Sau3AI GATC 5 cut(s) 16, 62, 67, 121, 447
Sau96I GGNCC 2 cut(s) 384, 427
ScaI AGTACT 1 cut(s) 376
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 1 cut(s) 728
SduI GDGCHC 3 cut(s) 57, 88, 608
SetI ASST 2 cut(s) 101, 676
SfaNI GCATC 3 cut(s) 18, 215, 541
SinI GGWCC 2 cut(s) 384, 427
SmiMI CAYNNNNRTG 3 cut(s) 147, 292, 569
SmlI CTYRAG 3 cut(s) 137, 411, 627
SmoI CTYRAG 3 cut(s) 137, 411, 627
Sse9I AATT 5 cut(s) 132, 354, 367, 496, 701
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 2 cut(s) 420, 520
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 726
TaaI ACNGT 6 cut(s) 40, 115, 229, 294, 361, 571
TaqI TCGA 1 cut(s) 535
TasI AATT 5 cut(s) 132, 354, 367, 496, 701
TatI WGTACW 3 cut(s) 198, 374, 623
TfiI GAWTC 1 cut(s) 791
Tru1I TTAA 2 cut(s) 704, 815
Tru9I TTAA 2 cut(s) 704, 815
TscAI CASTG 4 cut(s) 26, 118, 445, 658
TseFI GTSAC 2 cut(s) 152, 562
TseI GCWGC 1 cut(s) 543
Tsp45I GTSAC 2 cut(s) 152, 562
TspDTI ATGAA 2 cut(s) 380, 509
TspRI CASTG 4 cut(s) 26, 118, 445, 658
VpaK11BI GGWCC 2 cut(s) 384, 427
XapI RAATTY 1 cut(s) 496
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 801
XmnI GAANNNNTTC 1 cut(s) 586
ZrmI AGTACT 1 cut(s) 376
Zsp2I ATGCAT 2 cut(s) 6, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.