Rorug05G0034500

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002209
Physical Location & Seq
Forward (+)
2407381 .. 2407897
517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0034500.1

Sequence Viewer

Length: 447 bp
ATGGGTACCGGAAAGAAGAAAATTGACATAGAGCCGATCACCGACTATCAGTCTCGCATGGTGGCCTTCACCAGGAGACGAACGGGTCTCTTCAAAAAAGCCCAGAAGCTCGAAGAAATGTCTGGTTCATCCATCGCCATTGTTGTGATCTCCGAGACCGGTCAACCTTACCTCCATGGCTCTCCTTCCTTGCTTGAACGAGTTCTTGCCTCCAAAGCCTCTTCCTCCACAGTTACTGGCTCTTCCACCACCACAGCTGACACTGGCTCTTCCACCACCACCGCCACTACAGGATCTTTAGAAAGATTTAAGGCTCGGGTGAACTCTCAGATTGAAGCTTGTGCCACTCTGGAAGAAGCAGAGAGTTTGAAAAGGAAATTGTTGGAAATCAAAAGGAGGGCGGACGATAAAGTTTCTGGAAGTGATCTTCAATTTGTTCTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

148

Amino Acids

15.99

Weight (kDa)

9.48

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 12 - 57 1.3e-14 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 84
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 2 cut(s) 282, 401
AclWI GGATC 1 cut(s) 301
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 72
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 5 cut(s) 94, 197, 335, 370, 431
AhdI GACNNNNNGTC 1 cut(s) 49
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 3 cut(s) 109, 257, 338
AluI AGCT 3 cut(s) 109, 257, 338
Alw26I GTCTC 4 cut(s) 57, 70, 92, 149
AlwI GGATC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 315
AoxI GGCC 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 5
AsuHPI GGTGA 3 cut(s) 31, 61, 331
AvaI CYCGRG 1 cut(s) 315
BanI GGYRCC 1 cut(s) 5
BccI CCATC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 4 cut(s) 57, 70, 92, 149
BfmI CTRYAG 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 73
BmeRI GACNNNNNGTC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 92, 149
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 2 cut(s) 8, 158
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 241, 268
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 128
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 241, 268
BshFI GGCC 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 2 cut(s) 9, 159
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 4 cut(s) 57, 70, 92, 149
BsmBI CGTCTC 1 cut(s) 70
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 2 cut(s) 92, 149
BsoBI CYCGRG 1 cut(s) 315
Bsp143I GATC 4 cut(s) 36, 147, 293, 424
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 2 cut(s) 282, 401
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 1 cut(s) 301
BspQI GCTCTTC 2 cut(s) 247, 274
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 2 cut(s) 92, 149
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 241, 268
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 4 cut(s) 36, 147, 293, 424
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 4 cut(s) 95, 226, 247, 274
BstDEI CTNAG 1 cut(s) 327
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 128
BstKTI GATC 4 cut(s) 39, 150, 296, 427
BstMAI GTCTC 4 cut(s) 57, 70, 92, 149
BstMBI GATC 4 cut(s) 36, 147, 293, 424
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
BsuRI GGCC 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 6
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 58, 176
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 327
DpnI GATC 4 cut(s) 38, 149, 295, 426
DpnII GATC 4 cut(s) 36, 147, 293, 424
DrdI GACNNNNNNGTC 1 cut(s) 84
DriI GACNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 84
Eam1104I CTCTTC 4 cut(s) 95, 226, 247, 274
Eam1105I GACNNNNNGTC 1 cut(s) 49
EarI CTCTTC 4 cut(s) 95, 226, 247, 274
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 175
Eco31I GGTCTC 2 cut(s) 92, 149
Eco88I CYCGRG 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 2 cut(s) 61, 179
FaiI YATR 3 cut(s) 29, 59, 177
FatI CATG 2 cut(s) 57, 175
FokI GGATG 1 cut(s) 115
HaeIII GGCC 1 cut(s) 65
HapII CCGG 2 cut(s) 9, 159
Hin1II CATG 2 cut(s) 61, 179
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 336
HpaII CCGG 2 cut(s) 9, 159
HphI GGTGA 3 cut(s) 31, 61, 331
Hpy166II GTNNAC 2 cut(s) 164, 322
Hpy188I TCNGA 2 cut(s) 154, 330
Hpy188III TCNNGA 2 cut(s) 350, 417
Hpy8I GTNNAC 2 cut(s) 164, 322
HpyAV CCTTC 2 cut(s) 76, 195
HpyCH4III ACNGT 1 cut(s) 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 327
Hsp92II CATG 2 cut(s) 61, 179
KpnI GGTACC 1 cut(s) 9
Kzo9I GATC 4 cut(s) 36, 147, 293, 424
LguI GCTCTTC 2 cut(s) 247, 274
MaeIII GTNAC 1 cut(s) 232
MalI GATC 4 cut(s) 38, 149, 295, 426
MboI GATC 4 cut(s) 36, 147, 293, 424
MboII GAAGA 8 cut(s) 28, 82, 125, 213, 234, 261, 365, 419
MflI RGATCY 1 cut(s) 293
MluCI AATT 4 cut(s) 21, 377, 431, 442
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 5 cut(s) 182, 220, 229, 235, 390
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 2 cut(s) 309, 441
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 2 cut(s) 9, 159
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 215
NcoI CCATGG 1 cut(s) 175
NdeII GATC 4 cut(s) 36, 147, 293, 424
NlaIII CATG 2 cut(s) 61, 179
NlaIV GGNNCC 1 cut(s) 7
PciSI GCTCTTC 2 cut(s) 247, 274
PdmI GAANNNNTTC 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 143
SapI GCTCTTC 2 cut(s) 247, 274
SaqAI TTAA 2 cut(s) 309, 441
Sau3AI GATC 4 cut(s) 36, 147, 293, 424
ScrFI CCNGG 1 cut(s) 73
SetI ASST 5 cut(s) 111, 169, 174, 259, 340
SfcI CTRYAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 4 cut(s) 21, 377, 431, 442
SsiI CCGC 2 cut(s) 282, 401
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 232
TaqI TCGA 1 cut(s) 111
TasI AATT 4 cut(s) 21, 377, 431, 442
Tru1I TTAA 2 cut(s) 309, 441
Tru9I TTAA 2 cut(s) 309, 441
TscAI CASTG 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.