Rh6BG147400

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002209
Physical Location & Seq
Reverse (-)
23712750 .. 23718145
5396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG147400.1

Sequence Viewer

Length: 1515 bp
ATGGCGGAGAAAAATGGCGGTGGCGATCCACCAGCTGAGGAGGCAGAGGAGGAAGAGGAGAACCACGGTGGGGCTGACGGCGGTGCTTCACCGCAGAAAGAAACAGACGACGACGGCGAGAAGAAGAAGAAGAAGAAGCATTCAGATCCGGAGCTGCTGATGTGCTTGCTCCAACCTTGTCCCGCAGACTCCGATCCCGAATACATCGGCACTCGTCGCCTTCTTCTCTCTCGCAAGTCTCAGTCCTCCATTCTTCGCCGCCTAGATTGGAGATGCAATGGTAAGGGCTACGTGGCGTTCCGGAATTACATTCGGAGGCCGAGGAATTGGGAGAAGGATAGTCTGCGGACTCCGAGCCTCCAGACCACTCCGGGAAACAGTGGGCGATGGATTCAACCTCCGAGTCCACTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGGTAGTCAACCTTCATCTCGTAGAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGAGTCGACAGGGTGAACAACCTGCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGGTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCTTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAATGCAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACCGCCATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCTGTCACAACTGTGGAGTACCGAAGTTTTTCTCTGTTGGCTCGGGGCCCTGTTTTGCTGACATGTACTCAAGATGGAAGTTTGTCTTTCTTCAGTGTTGCGCTAGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTCTGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAATGTCTACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGGTCATCGATTTCCTGTTATTGGGGTTGCTTGGAACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTGTATGGAAGAGATCAAAGGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

504

Amino Acids

55.83

Weight (kDa)

8.53

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 186 - 277 1.1e-13 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 189 - 345 3.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 190 - 269 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 191 - 354 3.9e-14 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 192 - 499 2.2e-33 WDR5 beta-propeller domain
WDR55 PF24796 192 - 402 2.8e-11 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 195 - 312 9.9e-12 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 195 - 352 8.6e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 198 - 371 9.8e-16 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 205 - 356 2e-21 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 229 - 324 9.8e-10 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 230 - 264 4.8e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 231 - 486 7.8e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 239 - 339 1.2e-07 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 252 - 500 8.1e-07 RIG second beta-propeller
WD40_WDHD1_1st PF24817 437 - 501 6.5e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 438 - 502 5.5e-09 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 541, 1499
AccBSI CCGCTC 1 cut(s) 1074
AccI GTMKAC 2 cut(s) 517, 1353
AccIII TCCGGA 2 cut(s) 148, 300
AclWI GGATC 4 cut(s) 20, 140, 188, 668
AcoI YGGCCR 1 cut(s) 1373
AcsI RAATTY 2 cut(s) 1048, 1450
AcuI CTGAAG 1 cut(s) 1186
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 6 cut(s) 493, 752, 928, 1130, 1177, 1480
AfiI CCNNNNNNNGG 2 cut(s) 70, 1424
AflIII ACRYGT 2 cut(s) 1172, 1385
AgsI TTSAA 7 cut(s) 395, 598, 684, 801, 919, 1310, 1347
AhlI ACTAGT 1 cut(s) 494
AjnI CCWGG 1 cut(s) 442
AleI CACNNNNGTG 3 cut(s) 418, 844, 1121
AluBI AGCT 5 cut(s) 35, 154, 435, 615, 1399
AluI AGCT 5 cut(s) 35, 154, 435, 615, 1399
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 2 cut(s) 243, 673
AlwI GGATC 4 cut(s) 20, 140, 188, 668
Ama87I CYCGRG 2 cut(s) 487, 1152
Aor13HI TCCGGA 2 cut(s) 148, 300
AoxI GGCC 3 cut(s) 317, 1156, 1373
ApaI GGGCCC 1 cut(s) 1160
ApeKI GCWGC 2 cut(s) 154, 1095
ApoI RAATTY 2 cut(s) 1048, 1450
Asp700I GAANNNNTTC 1 cut(s) 1138
AspLEI GCGC 1 cut(s) 1213
AspS9I GGNCC 4 cut(s) 936, 979, 1156, 1157
AsuC2I CCSGG 2 cut(s) 372, 1280
AsuHPI GGTGA 3 cut(s) 81, 536, 851
AvaI CYCGRG 2 cut(s) 487, 1152
AvaII GGWCC 2 cut(s) 936, 979
BaeGI GKGCMC 2 cut(s) 609, 1160
BanII GRGCYC 2 cut(s) 617, 1160
BarI GAAGNNNNNNTAC 2 cut(s) 448, 480
Bbv12I GWGCWC 1 cut(s) 617
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 141, 1107
BccI CCATC 5 cut(s) 381, 446, 638, 1010, 1178
BceAI ACGGC 2 cut(s) 94, 130
BcgI CGANNNNNNTGC 2 cut(s) 747, 781
BciT130I CCWGG 1 cut(s) 444
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 2 cut(s) 372, 1280
BcoDI GTCTC 2 cut(s) 243, 673
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 3 cut(s) 263, 495, 1214
BfmI CTRYAG 1 cut(s) 1400
BfuAI ACCTGC 2 cut(s) 541, 1499
BglII AGATCT 1 cut(s) 619
BisI GCNGC 3 cut(s) 155, 259, 1096
BlsI GCNGC 3 cut(s) 156, 260, 1097
BmcAI AGTACT 2 cut(s) 493, 928
Bme1390I CCNGG 3 cut(s) 372, 444, 1280
Bme18I GGWCC 2 cut(s) 936, 979
BmeT110I CYCGRG 2 cut(s) 487, 1152
BmgT120I GGNCC 4 cut(s) 936, 979, 1156, 1157
BmiI GGNNCC 4 cut(s) 649, 1157, 1158, 1439
BmrFI CCNGG 3 cut(s) 372, 444, 1280
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 7 cut(s) 263, 570, 647, 703, 767, 1093, 1469
BmuI ACTGGG 1 cut(s) 939
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 1 cut(s) 344
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 674, 1164
BpuMI CCSGG 2 cut(s) 372, 1280
Bsa29I ATCGAT 1 cut(s) 1411
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 5 cut(s) 64, 320, 582, 975, 1441
BsaWI WCCGGW 2 cut(s) 148, 300
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 1424
Bse1I ACTGG 2 cut(s) 934, 997
Bse3DI GCAATG 4 cut(s) 283, 556, 753, 898
BseAI TCCGGA 2 cut(s) 148, 300
BseBI CCWGG 1 cut(s) 444
BseCI ATCGAT 1 cut(s) 1411
BseDI CCNNGG 5 cut(s) 64, 320, 582, 975, 1441
BseGI GGATG 2 cut(s) 457, 758
BseLI CCNNNNNNNGG 2 cut(s) 70, 1424
BseMI GCAATG 4 cut(s) 283, 556, 753, 898
BseMII CTCAG 4 cut(s) 27, 254, 503, 630
BseNI ACTGG 2 cut(s) 934, 997
BseRI GAGGAG 3 cut(s) 53, 62, 71
BseSI GKGCMC 2 cut(s) 609, 1160
BseXI GCAGC 2 cut(s) 141, 1107
BsgI GTGCAG 1 cut(s) 595
BshFI GGCC 3 cut(s) 319, 1158, 1375
BshVI ATCGAT 1 cut(s) 1411
BsiHKAI GWGCWC 1 cut(s) 617
BsiHKCI CYCGRG 2 cut(s) 487, 1152
BsiSI CCGG 5 cut(s) 149, 301, 371, 1279, 1332
BslFI GGGAC 2 cut(s) 165, 1033
BslI CCNNNNNNNGG 2 cut(s) 70, 1424
BsmAI GTCTC 2 cut(s) 243, 673
BsmFI GGGAC 2 cut(s) 165, 1033
BsmI GAATGC 2 cut(s) 139, 558
BsnI GGCC 3 cut(s) 319, 1158, 1375
BsoBI CYCGRG 2 cut(s) 487, 1152
Bsp120I GGGCCC 1 cut(s) 1156
Bsp1286I GDGCHC 3 cut(s) 609, 617, 1160
Bsp13I TCCGGA 2 cut(s) 148, 300
Bsp143I GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
Bsp19I CCATGG 2 cut(s) 975, 1441
BspANI GGCC 3 cut(s) 319, 1158, 1375
BspCNI CTCAG 4 cut(s) 28, 253, 504, 629
BspDI ATCGAT 1 cut(s) 1411
BspEI TCCGGA 2 cut(s) 148, 300
BspLI GGNNCC 4 cut(s) 649, 1157, 1158, 1439
BspMI ACCTGC 2 cut(s) 541, 1499
BspPI GGATC 4 cut(s) 20, 140, 188, 668
BsrBI CCGCTC 1 cut(s) 1074
BsrDI GCAATG 4 cut(s) 283, 556, 753, 898
BsrI ACTGG 2 cut(s) 934, 997
BssECI CCNNGG 5 cut(s) 64, 320, 582, 975, 1441
BssMI GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 975, 1441
Bst2UI CCWGG 1 cut(s) 444
Bst6I CTCTTC 3 cut(s) 48, 1242, 1490
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 3 cut(s) 167, 627, 1100
BstDEI CTNAG 4 cut(s) 36, 240, 512, 616
BstDSI CCRYGG 3 cut(s) 64, 975, 1441
BstF5I GGATG 2 cut(s) 457, 758
BstHHI GCGC 1 cut(s) 1213
BstKTI GATC 8 cut(s) 28, 148, 196, 571, 622, 676, 1002, 1502
BstMAI GTCTC 2 cut(s) 243, 673
BstMBI GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
BstMWI GCNNNNNNNGC 3 cut(s) 41, 216, 635
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 2 cut(s) 1176, 1389
BstSCI CCNGG 3 cut(s) 370, 442, 1278
BstSFI CTRYAG 1 cut(s) 1400
BstSLI GKGCMC 2 cut(s) 609, 1160
BstV1I GCAGC 2 cut(s) 141, 1107
BstX2I RGATCY 2 cut(s) 145, 619
BstYI RGATCY 2 cut(s) 145, 619
Bsu15I ATCGAT 1 cut(s) 1411
BsuRI GGCC 3 cut(s) 319, 1158, 1375
BsuTUI ATCGAT 1 cut(s) 1411
BtgI CCRYGG 3 cut(s) 64, 975, 1441
BtgZI GCGATG 1 cut(s) 400
BtsCI GGATG 2 cut(s) 457, 758
BtsIMutI CAGTG 5 cut(s) 385, 578, 663, 990, 1210
BveI ACCTGC 2 cut(s) 541, 1499
Cac8I GCNNGC 3 cut(s) 167, 627, 1100
CfoI GCGC 1 cut(s) 1213
Cfr13I GGNCC 4 cut(s) 936, 979, 1156, 1157
ClaI ATCGAT 1 cut(s) 1411
Csp6I GTAC 6 cut(s) 492, 751, 927, 1129, 1176, 1479
CviAII CATG 6 cut(s) 610, 976, 983, 1173, 1386, 1442
CviQI GTAC 6 cut(s) 492, 751, 927, 1129, 1176, 1479
DdeI CTNAG 4 cut(s) 36, 240, 512, 616
DpnI GATC 8 cut(s) 27, 147, 195, 570, 621, 675, 1001, 1501
DpnII GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
EaeI YGGCCR 1 cut(s) 1373
Eam1104I CTCTTC 3 cut(s) 48, 1242, 1490
EarI CTCTTC 3 cut(s) 48, 1242, 1490
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 975, 1441
Eco24I GRGCYC 2 cut(s) 617, 1160
Eco47I GGWCC 2 cut(s) 936, 979
Eco53kI GAGCTC 1 cut(s) 615
Eco57I CTGAAG 1 cut(s) 1186
Eco88I CYCGRG 2 cut(s) 487, 1152
EcoICRI GAGCTC 1 cut(s) 615
EcoO109I RGGNCCY 2 cut(s) 1156, 1157
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 975, 1441
EcoT22I ATGCAT 2 cut(s) 558, 815
EcoT38I GRGCYC 2 cut(s) 617, 1160
ErhI CCWWGG 2 cut(s) 975, 1441
FaeI CATG 6 cut(s) 613, 979, 986, 1176, 1389, 1445
FalI AAGNNNNNCTT 2 cut(s) 1180, 1212
FaqI GGGAC 2 cut(s) 165, 1033
FatI CATG 6 cut(s) 609, 975, 982, 1172, 1385, 1441
FauI CCCGC 2 cut(s) 190, 1079
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 2 cut(s) 517, 1353
Fnu4HI GCNGC 3 cut(s) 155, 259, 1096
FokI GGATG 2 cut(s) 464, 745
FriOI GRGCYC 2 cut(s) 617, 1160
Fsp4HI GCNGC 3 cut(s) 155, 259, 1096
FspBI CTAG 3 cut(s) 263, 495, 1214
GlaI GCGC 1 cut(s) 1212
GluI GCNGC 3 cut(s) 155, 259, 1096
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 3 cut(s) 319, 1158, 1375
HapII CCGG 5 cut(s) 149, 301, 371, 1279, 1332
HhaI GCGC 1 cut(s) 1213
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 6 cut(s) 613, 979, 986, 1176, 1389, 1445
Hin6I GCGC 1 cut(s) 1211
HinP1I GCGC 1 cut(s) 1211
HincII GTYRAC 2 cut(s) 461, 518
HindII GTYRAC 2 cut(s) 461, 518
HinfI GANTC 8 cut(s) 188, 349, 391, 403, 439, 514, 1234, 1343
HpaII CCGG 5 cut(s) 149, 301, 371, 1279, 1332
HphI GGTGA 3 cut(s) 81, 536, 851
Hpy166II GTNNAC 6 cut(s) 407, 461, 518, 527, 1354, 1393
Hpy188III TCNNGA 6 cut(s) 149, 197, 301, 361, 430, 1181
Hpy8I GTNNAC 6 cut(s) 407, 461, 518, 527, 1354, 1393
Hpy99I CGWCG 4 cut(s) 113, 116, 219, 431
HpyAV CCTTC 4 cut(s) 230, 328, 474, 690
HpyCH4IV ACGT 2 cut(s) 291, 426
HpyCH4V TGCA 9 cut(s) 276, 536, 556, 561, 576, 663, 758, 813, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 216, 635
HpyF3I CTNAG 4 cut(s) 36, 240, 512, 616
HpySE526I ACGT 2 cut(s) 291, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 6 cut(s) 613, 979, 986, 1176, 1389, 1445
HspAI GCGC 1 cut(s) 1211
Kpn2I TCCGGA 2 cut(s) 148, 300
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
LmnI GCTCC 3 cut(s) 151, 174, 635
Lsp1109I GCAGC 2 cut(s) 141, 1107
LweI GCATC 7 cut(s) 263, 570, 647, 703, 767, 1093, 1469
MaeI CTAG 3 cut(s) 263, 495, 1214
MaeII ACGT 2 cut(s) 291, 426
MaeIII GTNAC 6 cut(s) 422, 586, 704, 718, 967, 1114
MalI GATC 8 cut(s) 27, 147, 195, 570, 621, 675, 1001, 1501
MbiI CCGCTC 1 cut(s) 1074
MboI GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
MflI RGATCY 2 cut(s) 145, 619
MhlI GDGCHC 3 cut(s) 609, 617, 1160
MluCI AATT 8 cut(s) 304, 325, 684, 906, 919, 1048, 1253, 1450
MlyI GAGTC 6 cut(s) 182, 343, 412, 433, 523, 1228
MmeI TCCRAC 1 cut(s) 196
Mph1103I ATGCAT 2 cut(s) 558, 815
MroI TCCGGA 2 cut(s) 148, 300
MroXI GAANNNNTTC 1 cut(s) 1138
MseI TTAA 3 cut(s) 1256, 1367, 1513
MslI CAYNNNNRTG 5 cut(s) 418, 699, 844, 1121, 1386
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 5 cut(s) 149, 301, 371, 1279, 1332
MspR9I CCNGG 3 cut(s) 372, 444, 1280
Mva1269I GAATGC 2 cut(s) 139, 558
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 3 cut(s) 41, 216, 635
NciI CCSGG 2 cut(s) 372, 1280
NcoI CCATGG 2 cut(s) 975, 1441
NdeII GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
NlaIII CATG 6 cut(s) 613, 979, 986, 1176, 1389, 1445
NlaIV GGNNCC 4 cut(s) 649, 1157, 1158, 1439
NmeAIII GCCGAG 2 cut(s) 345, 1351
NmuCI GTSAC 3 cut(s) 422, 704, 1114
NsiI ATGCAT 2 cut(s) 558, 815
NspI RCATGY 2 cut(s) 1176, 1389
OliI CACNNNNGTG 3 cut(s) 418, 844, 1121
PaeR7I CTCGAG 1 cut(s) 487
PciI ACATGT 2 cut(s) 1172, 1385
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 2 cut(s) 139, 558
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 2 cut(s) 391, 1343
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 3 cut(s) 156, 260, 1097
PleI GAGTC 6 cut(s) 182, 343, 411, 433, 522, 1228
PpsI GAGTC 6 cut(s) 182, 343, 411, 433, 522, 1228
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 2 cut(s) 1172, 1385
Psp124BI GAGCTC 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 4 cut(s) 649, 1157, 1158, 1439
PspOMI GGGCCC 1 cut(s) 1156
PspPI GGNCC 4 cut(s) 936, 979, 1156, 1157
PspXI VCTCGAGB 1 cut(s) 487
PsuI RGATCY 2 cut(s) 145, 619
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 6 cut(s) 493, 752, 928, 1130, 1177, 1480
RsaNI GTAC 6 cut(s) 492, 751, 927, 1129, 1176, 1479
RseI CAYNNNNRTG 5 cut(s) 418, 699, 844, 1121, 1386
SacI GAGCTC 1 cut(s) 617
SalI GTCGAC 1 cut(s) 516
SaqAI TTAA 3 cut(s) 1256, 1367, 1513
SatI GCNGC 3 cut(s) 155, 259, 1096
Sau3AI GATC 8 cut(s) 25, 145, 193, 568, 619, 673, 999, 1499
Sau96I GGNCC 4 cut(s) 936, 979, 1156, 1157
ScaI AGTACT 2 cut(s) 493, 928
SchI GAGTC 6 cut(s) 182, 343, 412, 433, 523, 1228
ScrFI CCNGG 3 cut(s) 372, 444, 1280
SduI GDGCHC 3 cut(s) 609, 617, 1160
SfaNI GCATC 7 cut(s) 263, 570, 647, 703, 767, 1093, 1469
SfcI CTRYAG 1 cut(s) 1400
Sfr274I CTCGAG 1 cut(s) 487
SinI GGWCC 2 cut(s) 936, 979
SlaI CTCGAG 1 cut(s) 487
SmiMI CAYNNNNRTG 5 cut(s) 418, 699, 844, 1121, 1386
SmlI CTYRAG 3 cut(s) 487, 689, 1179
SmoI CTYRAG 3 cut(s) 487, 689, 1179
SpeI ACTAGT 1 cut(s) 494
Sse9I AATT 8 cut(s) 304, 325, 684, 906, 919, 1048, 1253, 1450
SspMI CTAG 3 cut(s) 263, 495, 1214
SstI GAGCTC 1 cut(s) 617
StyD4I CCNGG 3 cut(s) 370, 442, 1278
StyI CCWWGG 2 cut(s) 975, 1441
TaiI ACGT 2 cut(s) 294, 429
TaqI TCGA 5 cut(s) 429, 488, 517, 1087, 1411
TasI AATT 8 cut(s) 304, 325, 684, 906, 919, 1048, 1253, 1450
TatI WGTACW 4 cut(s) 491, 750, 926, 1175
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 391, 1343
Tru1I TTAA 3 cut(s) 1256, 1367, 1513
Tru9I TTAA 3 cut(s) 1256, 1367, 1513
TscAI CASTG 5 cut(s) 385, 578, 670, 997, 1210
TseFI GTSAC 3 cut(s) 422, 704, 1114
TseI GCWGC 2 cut(s) 154, 1095
Tsp45I GTSAC 3 cut(s) 422, 704, 1114
TspDTI ATGAA 3 cut(s) 456, 932, 1061
TspRI CASTG 5 cut(s) 385, 578, 670, 997, 1210
VpaK11BI GGWCC 2 cut(s) 936, 979
XapI RAATTY 2 cut(s) 1048, 1450
XceI RCATGY 2 cut(s) 1176, 1389
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XhoI CTCGAG 1 cut(s) 487
XmiI GTMKAC 2 cut(s) 517, 1353
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 3 cut(s) 263, 495, 1214
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 2 cut(s) 493, 928
Zsp2I ATGCAT 2 cut(s) 558, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.