Rh6AG147900

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002209
Physical Location & Seq
Forward (+)
23211922 .. 23217470
5549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG147900.1

Sequence Viewer

Length: 1059 bp
ATGATATATCAGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACCGCTATGGACCATGCTCACACTGGACAGATCATTTACAGTGGAGATGTGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAGCCACCTGATGTGAAATTTCAACCAAGCAAAAGTTTGCACTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTATGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATCTATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACTGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGATGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCACCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTGTCTTTCTTCTGTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTATGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAGTGTCTACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGGTCATCGATTTCCTGTTATTGGGGTTGCTTGGAACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTGTATGGAAGAGATCAAAGGCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

352

Amino Acids

39.25

Weight (kDa)

9.25

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 4 - 146 2.3e-06 WDR36/Utp21 first beta-propeller
EIF3I PF24805 4 - 150 1.5e-06 EIF3I
Beta-prop_THOC3 PF25174 5 - 160 3.5e-13 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 5 - 216 3.3e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 16 - 150 3.1e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 58 - 174 7.9e-11 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 82 - 171 8.2e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 87 - 347 1.7e-21 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 87 - 158 1.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 87 - 159 3.8e-11 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 111 - 156 2.3e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 112 - 146 1.4e-09 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 285 - 349 4.2e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 286 - 350 3.9e-09 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1043
AccBSI CCGCTC 2 cut(s) 174, 672
AccI GTMKAC 1 cut(s) 897
AciI CCGC 3 cut(s) 72, 172, 670
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 917
AcsI RAATTY 4 cut(s) 148, 221, 646, 994
AfaI GTAC 5 cut(s) 28, 398, 526, 728, 1024
AfiI CCNNNNNNNGG 1 cut(s) 968
AflIII ACRYGT 1 cut(s) 929
AgsI TTSAA 7 cut(s) 19, 227, 330, 447, 517, 854, 891
AleI CACNNNNGTG 2 cut(s) 490, 719
AluBI AGCT 2 cut(s) 261, 943
AluI AGCT 2 cut(s) 261, 943
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 314
AoxI GGCC 2 cut(s) 451, 917
ApeKI GCWGC 2 cut(s) 195, 693
ApoI RAATTY 4 cut(s) 148, 221, 646, 994
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 4 cut(s) 36, 79, 534, 577
AsuC2I CCSGG 1 cut(s) 824
AsuHPI GGTGA 2 cut(s) 497, 668
AvaII GGWCC 4 cut(s) 36, 79, 534, 577
BaeGI GKGCMC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 207, 705
BccI CCATC 3 cut(s) 284, 608, 613
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BcnI CCSGG 1 cut(s) 824
BcoDI GTCTC 1 cut(s) 319
BfmI CTRYAG 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 1043
BglII AGATCT 1 cut(s) 265
BisI GCNGC 2 cut(s) 196, 694
BlsI GCNGC 2 cut(s) 197, 695
BmcAI AGTACT 2 cut(s) 28, 526
Bme1390I CCNGG 1 cut(s) 824
Bme18I GGWCC 4 cut(s) 36, 79, 534, 577
BmgT120I GGNCC 4 cut(s) 36, 79, 534, 577
BmiI GGNNCC 2 cut(s) 295, 983
BmrFI CCNGG 1 cut(s) 824
BmrI ACTGGG 2 cut(s) 39, 537
BmsI GCATC 6 cut(s) 193, 293, 349, 413, 691, 1013
BmuI ACTGGG 2 cut(s) 39, 537
BpmI CTGGAG 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 824
Bsa29I ATCGAT 1 cut(s) 955
BsaJI CCNNGG 1 cut(s) 985
Bsc4I CCNNNNNNNGG 1 cut(s) 968
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 5 cut(s) 34, 97, 532, 595, 710
Bse3DI GCAATG 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 955
BseDI CCNNGG 1 cut(s) 985
BseGI GGATG 2 cut(s) 404, 624
BseLI CCNNNNNNNGG 1 cut(s) 968
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 5 cut(s) 34, 97, 532, 595, 710
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 2 cut(s) 207, 705
BshFI GGCC 2 cut(s) 453, 919
BshVI ATCGAT 1 cut(s) 955
BsiHKAI GWGCWC 1 cut(s) 263
BsiSI CCGG 3 cut(s) 679, 823, 876
BslI CCNNNNNNNGG 1 cut(s) 968
BsmAI GTCTC 1 cut(s) 319
BsnI GGCC 2 cut(s) 453, 919
Bsp1286I GDGCHC 2 cut(s) 255, 263
Bsp143I GATC 5 cut(s) 99, 265, 319, 597, 1043
Bsp19I CCATGG 1 cut(s) 985
BspACI CCGC 3 cut(s) 72, 172, 670
BspANI GGCC 2 cut(s) 453, 919
BspCNI CTCAG 1 cut(s) 275
BspDI ATCGAT 1 cut(s) 955
BspLI GGNNCC 2 cut(s) 295, 983
BspMI ACCTGC 1 cut(s) 1043
BspPI GGATC 1 cut(s) 314
BsrBI CCGCTC 2 cut(s) 174, 672
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 5 cut(s) 34, 97, 532, 595, 710
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 1 cut(s) 985
BssMI GATC 5 cut(s) 99, 265, 319, 597, 1043
BssT1I CCWWGG 1 cut(s) 985
Bst4CI ACNGT 8 cut(s) 62, 110, 304, 313, 492, 560, 721, 1027
Bst6I CTCTTC 2 cut(s) 786, 1034
BstC8I GCNNGC 3 cut(s) 200, 273, 698
BstDEI CTNAG 1 cut(s) 262
BstDSI CCRYGG 1 cut(s) 985
BstF5I GGATG 2 cut(s) 404, 624
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 5 cut(s) 102, 268, 322, 600, 1046
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 5 cut(s) 99, 265, 319, 597, 1043
BstMWI GCNNNNNNNGC 2 cut(s) 250, 281
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 1 cut(s) 822
BstSFI CTRYAG 1 cut(s) 944
BstSLI GKGCMC 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 207, 705
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 955
BsuRI GGCC 2 cut(s) 453, 919
BsuTUI ATCGAT 1 cut(s) 955
BtgI CCRYGG 1 cut(s) 985
BtsCI GGATG 2 cut(s) 404, 624
BtsIMutI CAGTG 4 cut(s) 90, 115, 309, 588
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 3 cut(s) 200, 273, 698
CfoI GCGC 1 cut(s) 757
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 4 cut(s) 36, 79, 534, 577
ClaI ATCGAT 1 cut(s) 955
Csp6I GTAC 5 cut(s) 27, 397, 525, 727, 1023
CviAII CATG 5 cut(s) 83, 256, 581, 930, 986
CviJI RGCY 6 cut(s) 208, 261, 453, 827, 919, 943
CviKI_1 RGCY 6 cut(s) 208, 261, 453, 827, 919, 943
CviQI GTAC 5 cut(s) 27, 397, 525, 727, 1023
DdeI CTNAG 1 cut(s) 262
DpnI GATC 5 cut(s) 101, 267, 321, 599, 1045
DpnII GATC 5 cut(s) 99, 265, 319, 597, 1043
EaeI YGGCCR 1 cut(s) 917
Eam1104I CTCTTC 2 cut(s) 786, 1034
EarI CTCTTC 2 cut(s) 786, 1034
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 985
Eco147I AGGCCT 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 263
Eco47I GGWCC 4 cut(s) 36, 79, 534, 577
Eco53kI GAGCTC 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 261
EcoRI GAATTC 2 cut(s) 148, 646
EcoT14I CCWWGG 1 cut(s) 985
EcoT22I ATGCAT 1 cut(s) 461
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 985
FaeI CATG 5 cut(s) 86, 259, 584, 933, 989
FalI AAGNNNNNCTT 2 cut(s) 724, 756
FatI CATG 5 cut(s) 82, 255, 580, 929, 985
FauI CCCGC 2 cut(s) 179, 677
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 2 cut(s) 196, 694
FokI GGATG 2 cut(s) 391, 631
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 196, 694
GlaI GCGC 1 cut(s) 756
GluI GCNGC 2 cut(s) 196, 694
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 2 cut(s) 453, 919
HapII CCGG 3 cut(s) 679, 823, 876
HhaI GCGC 1 cut(s) 757
Hin1II CATG 5 cut(s) 86, 259, 584, 933, 989
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HinfI GANTC 2 cut(s) 778, 887
HpaII CCGG 3 cut(s) 679, 823, 876
HphI GGTGA 2 cut(s) 497, 668
Hpy166II GTNNAC 2 cut(s) 898, 937
Hpy188I TCNGA 5 cut(s) 265, 289, 319, 345, 1012
Hpy8I GTNNAC 2 cut(s) 898, 937
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 8 cut(s) 62, 110, 304, 313, 492, 560, 721, 1027
HpyCH4V TGCA 4 cut(s) 244, 309, 404, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 281
HpyF3I CTNAG 1 cut(s) 262
Hsp92II CATG 5 cut(s) 86, 259, 584, 933, 989
HspAI GCGC 1 cut(s) 755
Kzo9I GATC 5 cut(s) 99, 265, 319, 597, 1043
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 2 cut(s) 207, 705
LweI GCATC 6 cut(s) 193, 293, 349, 413, 691, 1013
MaeIII GTNAC 6 cut(s) 67, 118, 350, 364, 565, 712
MalI GATC 5 cut(s) 101, 267, 321, 599, 1045
MbiI CCGCTC 2 cut(s) 174, 672
MboI GATC 5 cut(s) 99, 265, 319, 597, 1043
MboII GAAGA 4 cut(s) 736, 773, 896, 1051
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 255, 263
MluCI AATT 8 cut(s) 19, 148, 221, 330, 517, 646, 797, 994
MlyI GAGTC 1 cut(s) 772
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 4 cut(s) 27, 333, 418, 525
Mph1103I ATGCAT 1 cut(s) 461
MseI TTAA 3 cut(s) 800, 911, 1057
MslI CAYNNNNRTG 4 cut(s) 345, 490, 719, 930
MspI CCGG 3 cut(s) 679, 823, 876
MspR9I CCNGG 1 cut(s) 824
MwoI GCNNNNNNNGC 2 cut(s) 250, 281
NciI CCSGG 1 cut(s) 824
NcoI CCATGG 1 cut(s) 985
NdeII GATC 5 cut(s) 99, 265, 319, 597, 1043
NlaIII CATG 5 cut(s) 86, 259, 584, 933, 989
NlaIV GGNNCC 2 cut(s) 295, 983
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 3 cut(s) 118, 350, 712
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 1 cut(s) 933
OliI CACNNNNGTG 2 cut(s) 490, 719
PceI AGGCCT 1 cut(s) 453
PciI ACATGT 1 cut(s) 929
PfeI GAWTC 1 cut(s) 887
PkrI GCNGC 2 cut(s) 197, 695
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
PscI ACATGT 1 cut(s) 929
Psp124BI GAGCTC 1 cut(s) 263
PspN4I GGNNCC 2 cut(s) 295, 983
PspPI GGNCC 4 cut(s) 36, 79, 534, 577
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 5 cut(s) 28, 398, 526, 728, 1024
RsaNI GTAC 5 cut(s) 27, 397, 525, 727, 1023
RseI CAYNNNNRTG 4 cut(s) 345, 490, 719, 930
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 3 cut(s) 800, 911, 1057
SatI GCNGC 2 cut(s) 196, 694
Sau3AI GATC 5 cut(s) 99, 265, 319, 597, 1043
Sau96I GGNCC 4 cut(s) 36, 79, 534, 577
ScaI AGTACT 2 cut(s) 28, 526
SchI GAGTC 1 cut(s) 772
ScrFI CCNGG 1 cut(s) 824
SduI GDGCHC 2 cut(s) 255, 263
SetI ASST 7 cut(s) 15, 214, 263, 299, 772, 945, 1057
SfaNI GCATC 6 cut(s) 193, 293, 349, 413, 691, 1013
SfcI CTRYAG 1 cut(s) 944
SinI GGWCC 4 cut(s) 36, 79, 534, 577
SmiMI CAYNNNNRTG 4 cut(s) 345, 490, 719, 930
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 8 cut(s) 19, 148, 221, 330, 517, 646, 797, 994
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 3 cut(s) 72, 172, 670
SstI GAGCTC 1 cut(s) 263
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 822
StyI CCWWGG 1 cut(s) 985
TaaI ACNGT 8 cut(s) 62, 110, 304, 313, 492, 560, 721, 1027
TaqI TCGA 3 cut(s) 187, 685, 955
TasI AATT 8 cut(s) 19, 148, 221, 330, 517, 646, 797, 994
TatI WGTACW 3 cut(s) 26, 396, 524
TfiI GAWTC 1 cut(s) 887
Tru1I TTAA 3 cut(s) 800, 911, 1057
Tru9I TTAA 3 cut(s) 800, 911, 1057
TscAI CASTG 4 cut(s) 97, 115, 316, 595
TseFI GTSAC 3 cut(s) 118, 350, 712
TseI GCWGC 2 cut(s) 195, 693
Tsp45I GTSAC 3 cut(s) 118, 350, 712
TspDTI ATGAA 4 cut(s) 32, 161, 530, 659
TspRI CASTG 4 cut(s) 97, 115, 316, 595
VpaK11BI GGWCC 4 cut(s) 36, 79, 534, 577
XapI RAATTY 4 cut(s) 148, 221, 646, 994
XceI RCATGY 1 cut(s) 933
XcmI CCANNNNNNNNNTGG 2 cut(s) 89, 587
XmiI GTMKAC 1 cut(s) 897
ZrmI AGTACT 2 cut(s) 28, 526
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.