Rh6AG148100

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002209
Physical Location & Seq
Forward (+)
23272310 .. 23284673
12364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG148100.1

Sequence Viewer

Length: 1065 bp
ATGAATGGAAGTCCAGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACCGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAGCCACCTGATGCTGCTTGATGCATTAGTGGGCGATGGATTCAACCTCCGAGTCCACTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGTGCATAGTGGCAGTCAACCTTCATCTCGTACAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCAACGGGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCGTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTTCATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAACGTAGACAAAGAAATTACGTTACTGCTATATATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGTTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTTTTTCTGTTGGCTCGGGCCCTTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTATGCTTTCCCTTGAGAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAGTGTATACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

354

Amino Acids

38.89

Weight (kDa)

8.42

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 111 - 195 1.7e-11 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 112 - 228 9.5e-13 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 115 - 184 3.6e-07 WDR55
Beta-prop_WDR5 PF25175 121 - 227 3.2e-15 WDR5 beta-propeller domain
WD40_Prp19 PF24814 122 - 215 3.9e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 122 - 264 1.3e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 123 - 221 8.4e-13 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 123 - 187 4.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 124 - 223 7.6e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 142 - 219 2.6e-06 WDR3 second beta-propeller domain
WD40 PF00400 146 - 181 2.6e-10 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 146 - 192 9.4e-07 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 140
AccI GTMKAC 2 cut(s) 648, 1008
AciI CCGC 3 cut(s) 75, 138, 336
AclWI GGATC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 1028
AcsI RAATTY 1 cut(s) 748
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 4 cut(s) 31, 303, 321, 830
AflIII ACRYGT 1 cut(s) 1040
AgsI TTSAA 5 cut(s) 22, 214, 435, 552, 1002
AhlI ACTAGT 1 cut(s) 322
AjnI CCWGG 1 cut(s) 261
AleI CACNNNNGTG 3 cut(s) 237, 595, 821
AluBI AGCT 3 cut(s) 254, 366, 1054
AluI AGCT 3 cut(s) 254, 366, 1054
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 424
AlwI GGATC 1 cut(s) 419
Ama87I CYCGRG 2 cut(s) 315, 852
AoxI GGCC 3 cut(s) 556, 855, 1028
ApaI GGGCCC 1 cut(s) 859
ApeKI GCWGC 3 cut(s) 161, 184, 795
ApoI RAATTY 1 cut(s) 748
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 5 cut(s) 39, 82, 679, 855, 856
AsuC2I CCSGG 1 cut(s) 935
AsuHPI GGTGA 1 cut(s) 602
AvaI CYCGRG 2 cut(s) 315, 852
AvaII GGWCC 3 cut(s) 39, 82, 679
BaeGI GKGCMC 2 cut(s) 360, 859
BanII GRGCYC 2 cut(s) 368, 859
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 3 cut(s) 171, 173, 807
BccI CCATC 4 cut(s) 113, 200, 389, 710
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 1 cut(s) 263
BcnI CCSGG 1 cut(s) 935
BcoDI GTCTC 1 cut(s) 424
BcuI ACTAGT 1 cut(s) 322
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 1055
BglII AGATCT 1 cut(s) 370
BisI GCNGC 3 cut(s) 162, 185, 796
BlsI GCNGC 3 cut(s) 163, 186, 797
BmcAI AGTACT 2 cut(s) 31, 321
Bme1390I CCNGG 2 cut(s) 263, 935
Bme18I GGWCC 3 cut(s) 39, 82, 679
BmeT110I CYCGRG 2 cut(s) 315, 852
BmgT120I GGNCC 5 cut(s) 39, 82, 679, 855, 856
BmiI GGNNCC 2 cut(s) 400, 857
BmrFI CCNGG 2 cut(s) 263, 935
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 7 cut(s) 159, 171, 181, 398, 454, 518, 793
BmuI ACTGGG 1 cut(s) 42
BplI GAGNNNNNCTC 2 cut(s) 243, 275
BpmI CTGGAG 1 cut(s) 795
BpuEI CTTGAG 2 cut(s) 425, 983
BpuMI CCSGG 1 cut(s) 935
BsaHI GRCGYC 1 cut(s) 245
Bse1I ACTGG 5 cut(s) 14, 37, 100, 697, 812
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 263
BseGI GGATG 2 cut(s) 276, 509
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 5 cut(s) 14, 37, 100, 697, 812
BseSI GKGCMC 2 cut(s) 360, 859
BseXI GCAGC 3 cut(s) 171, 173, 807
BshFI GGCC 3 cut(s) 558, 857, 1030
BsiHKAI GWGCWC 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 315, 852
BsiSI CCGG 2 cut(s) 934, 987
BslFI GGGAC 2 cut(s) 136, 733
BsmAI GTCTC 1 cut(s) 424
BsmFI GGGAC 2 cut(s) 136, 733
BsnI GGCC 3 cut(s) 558, 857, 1030
BsoBI CYCGRG 2 cut(s) 315, 852
Bsp120I GGGCCC 1 cut(s) 855
Bsp1286I GDGCHC 3 cut(s) 360, 368, 859
Bsp143I GATC 4 cut(s) 102, 370, 424, 699
BspACI CCGC 3 cut(s) 75, 138, 336
BspANI GGCC 3 cut(s) 558, 857, 1030
BspCNI CTCAG 1 cut(s) 380
BspLI GGNNCC 2 cut(s) 400, 857
BspPI GGATC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 504
BsrI ACTGG 5 cut(s) 14, 37, 100, 697, 812
BssMI GATC 4 cut(s) 102, 370, 424, 699
BssNAI GTATAC 1 cut(s) 1009
BssNI GRCGYC 1 cut(s) 245
Bst1107I GTATAC 1 cut(s) 1009
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 6 cut(s) 65, 409, 418, 532, 597, 823
Bst6I CTCTTC 1 cut(s) 897
BstACI GRCGYC 1 cut(s) 245
BstC8I GCNNGC 4 cut(s) 166, 340, 378, 800
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 276, 509
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 4 cut(s) 105, 373, 427, 702
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 4 cut(s) 102, 370, 424, 699
BstMWI GCNNNNNNNGC 2 cut(s) 386, 863
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 1 cut(s) 1044
BstSCI CCNGG 2 cut(s) 261, 933
BstSFI CTRYAG 1 cut(s) 1055
BstSLI GKGCMC 2 cut(s) 360, 859
BstV1I GCAGC 3 cut(s) 171, 173, 807
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BstZ17I GTATAC 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 558, 857, 1030
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 276, 509
BtsIMutI CAGTG 3 cut(s) 93, 414, 690
Cac8I GCNNGC 4 cut(s) 166, 340, 378, 800
CfoI GCGC 1 cut(s) 868
Cfr13I GGNCC 5 cut(s) 39, 82, 679, 855, 856
CseI GACGC 1 cut(s) 331
Csp6I GTAC 4 cut(s) 30, 302, 320, 829
CviAII CATG 4 cut(s) 86, 361, 683, 1041
CviQI GTAC 4 cut(s) 30, 302, 320, 829
DdeI CTNAG 1 cut(s) 367
DpnI GATC 4 cut(s) 104, 372, 426, 701
DpnII GATC 4 cut(s) 102, 370, 424, 699
EaeI YGGCCR 1 cut(s) 1028
Eam1104I CTCTTC 1 cut(s) 897
EarI CTCTTC 1 cut(s) 897
Ecl136II GAGCTC 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 558
Eco24I GRGCYC 2 cut(s) 368, 859
Eco47I GGWCC 3 cut(s) 39, 82, 679
Eco53kI GAGCTC 1 cut(s) 366
Eco88I CYCGRG 2 cut(s) 315, 852
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 856
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 1 cut(s) 261
EcoT22I ATGCAT 2 cut(s) 196, 566
EcoT38I GRGCYC 2 cut(s) 368, 859
FaeI CATG 4 cut(s) 89, 364, 686, 1044
FaqI GGGAC 2 cut(s) 136, 733
FatI CATG 4 cut(s) 85, 360, 682, 1040
FauI CCCGC 1 cut(s) 145
FblI GTMKAC 2 cut(s) 648, 1008
Fnu4HI GCNGC 3 cut(s) 162, 185, 796
FokI GGATG 2 cut(s) 283, 496
FriOI GRGCYC 2 cut(s) 368, 859
Fsp4HI GCNGC 3 cut(s) 162, 185, 796
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 867
GluI GCNGC 3 cut(s) 162, 185, 796
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 3 cut(s) 558, 857, 1030
HapII CCGG 2 cut(s) 934, 987
HgaI GACGC 1 cut(s) 331
HhaI GCGC 1 cut(s) 868
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 4 cut(s) 89, 364, 686, 1044
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HincII GTYRAC 2 cut(s) 289, 346
HindII GTYRAC 2 cut(s) 289, 346
HinfI GANTC 5 cut(s) 210, 222, 258, 889, 998
HpaII CCGG 2 cut(s) 934, 987
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 6 cut(s) 226, 289, 346, 649, 1009, 1048
Hpy188I TCNGA 5 cut(s) 221, 370, 394, 424, 450
Hpy188III TCNNGA 1 cut(s) 249
Hpy8I GTNNAC 6 cut(s) 226, 289, 346, 649, 1009, 1048
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 6 cut(s) 65, 409, 418, 532, 597, 823
HpyCH4IV ACGT 3 cut(s) 245, 645, 662
HpyCH4V TGCA 5 cut(s) 194, 276, 414, 509, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 863
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 3 cut(s) 245, 645, 662
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 4 cut(s) 89, 364, 686, 1044
HspAI GCGC 1 cut(s) 866
Kzo9I GATC 4 cut(s) 102, 370, 424, 699
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 3 cut(s) 171, 173, 807
LweI GCATC 7 cut(s) 159, 171, 181, 398, 454, 518, 793
MaeI CTAG 1 cut(s) 323
MaeII ACGT 3 cut(s) 245, 645, 662
MaeIII GTNAC 6 cut(s) 70, 241, 455, 469, 663, 814
MalI GATC 4 cut(s) 104, 372, 426, 701
MbiI CCGCTC 1 cut(s) 140
MboI GATC 4 cut(s) 102, 370, 424, 699
MboII GAAGA 2 cut(s) 884, 1007
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 3 cut(s) 360, 368, 859
MluCI AATT 5 cut(s) 22, 435, 657, 748, 908
MlyI GAGTC 3 cut(s) 231, 252, 883
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 4 cut(s) 30, 227, 438, 523
Mph1103I ATGCAT 2 cut(s) 196, 566
MseI TTAA 3 cut(s) 911, 1022, 1063
MslI CAYNNNNRTG 5 cut(s) 237, 450, 595, 821, 1041
MspI CCGG 2 cut(s) 934, 987
MspR9I CCNGG 2 cut(s) 263, 935
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 386, 863
NciI CCSGG 1 cut(s) 935
NdeII GATC 4 cut(s) 102, 370, 424, 699
NlaIII CATG 4 cut(s) 89, 364, 686, 1044
NlaIV GGNNCC 2 cut(s) 400, 857
NmeAIII GCCGAG 1 cut(s) 1006
NmuCI GTSAC 3 cut(s) 241, 455, 814
NsiI ATGCAT 2 cut(s) 196, 566
NspI RCATGY 1 cut(s) 1044
OliI CACNNNNGTG 3 cut(s) 237, 595, 821
PaeR7I CTCGAG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 558
PciI ACATGT 1 cut(s) 1040
PfeI GAWTC 2 cut(s) 210, 998
PkrI GCNGC 3 cut(s) 163, 186, 797
PleI GAGTC 3 cut(s) 230, 252, 883
PpsI GAGTC 3 cut(s) 230, 252, 883
PscI ACATGT 1 cut(s) 1040
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 400, 857
PspOMI GGGCCC 1 cut(s) 855
PspPI GGNCC 5 cut(s) 39, 82, 679, 855, 856
PspXI VCTCGAGB 1 cut(s) 315
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 4 cut(s) 31, 303, 321, 830
RsaNI GTAC 4 cut(s) 30, 302, 320, 829
RseI CAYNNNNRTG 5 cut(s) 237, 450, 595, 821, 1041
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 911, 1022, 1063
SatI GCNGC 3 cut(s) 162, 185, 796
Sau3AI GATC 4 cut(s) 102, 370, 424, 699
Sau96I GGNCC 5 cut(s) 39, 82, 679, 855, 856
ScaI AGTACT 2 cut(s) 31, 321
SchI GAGTC 3 cut(s) 231, 252, 883
ScrFI CCNGG 2 cut(s) 263, 935
SduI GDGCHC 3 cut(s) 360, 368, 859
SfaNI GCATC 7 cut(s) 159, 171, 181, 398, 454, 518, 793
SfcI CTRYAG 1 cut(s) 1055
Sfr274I CTCGAG 1 cut(s) 315
SinI GGWCC 3 cut(s) 39, 82, 679
SlaI CTCGAG 1 cut(s) 315
SmiMI CAYNNNNRTG 5 cut(s) 237, 450, 595, 821, 1041
SmlI CTYRAG 3 cut(s) 315, 440, 962
SmoI CTYRAG 3 cut(s) 315, 440, 962
SpeI ACTAGT 1 cut(s) 322
Sse9I AATT 5 cut(s) 22, 435, 657, 748, 908
SseBI AGGCCT 1 cut(s) 558
SsiI CCGC 3 cut(s) 75, 138, 336
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 558
StyD4I CCNGG 2 cut(s) 261, 933
TaaI ACNGT 6 cut(s) 65, 409, 418, 532, 597, 823
TaiI ACGT 3 cut(s) 248, 648, 665
TaqI TCGA 4 cut(s) 153, 248, 316, 787
TasI AATT 5 cut(s) 22, 435, 657, 748, 908
TatI WGTACW 2 cut(s) 29, 319
TfiI GAWTC 2 cut(s) 210, 998
Tru1I TTAA 3 cut(s) 911, 1022, 1063
Tru9I TTAA 3 cut(s) 911, 1022, 1063
TscAI CASTG 3 cut(s) 100, 421, 697
TseFI GTSAC 3 cut(s) 241, 455, 814
TseI GCWGC 3 cut(s) 161, 184, 795
Tsp45I GTSAC 3 cut(s) 241, 455, 814
TspDTI ATGAA 5 cut(s) 17, 35, 284, 493, 761
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 3 cut(s) 100, 421, 697
VpaK11BI GGWCC 3 cut(s) 39, 82, 679
XapI RAATTY 1 cut(s) 748
XceI RCATGY 1 cut(s) 1044
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 689
XhoI CTCGAG 1 cut(s) 315
XmiI GTMKAC 2 cut(s) 648, 1008
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 246
ZrmI AGTACT 2 cut(s) 31, 321
Zsp2I ATGCAT 2 cut(s) 196, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.