Rw6G012620

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002209
Physical Location & Seq
Reverse (-)
23087410 .. 23092836
5427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G012620.1

Sequence Viewer

Length: 1512 bp
ATGGCGGAGAAAAATGGCGGAGGAGATCCACCAGCTGAGGAGGCAGAGGAGGAAGAGGAGAACCACGGTGGCGCTGACGGCGGTGCTTCACCGCAGAAAGAAACAGACGACGACGGCGAGAAGAAGAAGAAGAAGCATTCAGATCCGGAGCTGCTGATGTGCTTGCTCCAACCTTGTCCCGCAGACTCCGATCCCGAATACATCGGCATTCGTCGCCTTCTTCTCTCTCGCAAGTCTCAGTCCTCCATTCTTCGCCGCCTAGATTGGAGATGCAATGGTAAGGGCTACGTGGCGTTCCGGAATTACATTCGGAGGCCGAGGAATTGGGAGAAGGATAGTCTGCGGACTCCGAGCCTCCAGACGACTCCGGGAAACAGTGGGCGATGGATTCAACCTCCGAGTCCACTCTCACTTTTGTGTGACGTCGAGAGCTGGACTCCTGGCAGGGATGGTAGTCAACCTTCATCTCGTAGAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCTTGCGTCGACAGGGTGAACAACCTGCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGGTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCTTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAATAACAACTTCCTTTCAGTTGGCAATGCAGACAAAGAAATTACGGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTGACAGTCAAGTTACTGCTATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCACACAGGAGTATTATCCCGCTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAACTGTGGAGTACCGAAGTTTTTCTCTGTTGGCTCGGGGTCCTGTTTTGCTGACATGTACTCAAGATGGAAGTTTGTCTTTCTTCAGTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTCTGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAATGTCTACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGGGTCATCGATTTCCTGTTATAGGGGTTGCTTGGAACCATGGAGAGAATTTATTAGCATCATCTGATTTCTATGGTACGGTTATTTTATGGAAGAGATCAAAGGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

503

Amino Acids

55.85

Weight (kDa)

8.42

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 185 - 276 1.1e-13 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 188 - 344 3.9e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 189 - 268 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 191 - 498 2.5e-33 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 191 - 361 2.6e-14 WDHD1 first WD40 domain
WDR55 PF24796 191 - 401 3e-11 WDR55
WD40_Prp19 PF24814 194 - 351 8.6e-15 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 194 - 308 1.2e-11 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 197 - 370 9.7e-16 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 204 - 355 1.5e-21 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 228 - 323 9.8e-10 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 229 - 263 4.8e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 230 - 484 8e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 238 - 339 1.1e-07 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 436 - 500 1.4e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 437 - 501 1.2e-08 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 538
AccBSI CCGCTC 1 cut(s) 1071
AccI GTMKAC 2 cut(s) 514, 1350
AccIII TCCGGA 2 cut(s) 145, 297
AciI CCGC 9 cut(s) 5, 18, 81, 92, 180, 256, 343, 505, 1069
AclWI GGATC 4 cut(s) 20, 137, 185, 665
AcoI YGGCCR 1 cut(s) 1370
AcsI RAATTY 2 cut(s) 1045, 1447
AcuI CTGAAG 1 cut(s) 1183
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 6 cut(s) 490, 749, 925, 1127, 1174, 1477
AfiI CCNNNNNNNGG 1 cut(s) 1421
AflIII ACRYGT 2 cut(s) 1169, 1382
AgsI TTSAA 7 cut(s) 392, 595, 681, 798, 916, 1307, 1344
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 439
AleI CACNNNNGTG 3 cut(s) 415, 841, 1118
AluBI AGCT 5 cut(s) 35, 151, 432, 612, 1396
AluI AGCT 5 cut(s) 35, 151, 432, 612, 1396
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 2 cut(s) 240, 670
AlwI GGATC 4 cut(s) 20, 137, 185, 665
Ama87I CYCGRG 2 cut(s) 484, 1149
Aor13HI TCCGGA 2 cut(s) 145, 297
AoxI GGCC 2 cut(s) 314, 1370
ApeKI GCWGC 2 cut(s) 151, 1092
ApoI RAATTY 2 cut(s) 1045, 1447
Asp700I GAANNNNTTC 1 cut(s) 1135
AspLEI GCGC 2 cut(s) 74, 1210
AspS9I GGNCC 3 cut(s) 933, 976, 1154
AsuC2I CCSGG 2 cut(s) 369, 1277
AsuHPI GGTGA 3 cut(s) 81, 533, 848
AvaI CYCGRG 2 cut(s) 484, 1149
AvaII GGWCC 3 cut(s) 933, 976, 1154
BaeGI GKGCMC 1 cut(s) 606
BanII GRGCYC 1 cut(s) 614
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
Bbv12I GWGCWC 1 cut(s) 614
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 138, 1104
BccI CCATC 5 cut(s) 378, 443, 635, 1007, 1175
BceAI ACGGC 2 cut(s) 94, 130
BcgI CGANNNNNNTGC 2 cut(s) 744, 778
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 2 cut(s) 369, 1277
BcoDI GTCTC 2 cut(s) 240, 670
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 2 cut(s) 260, 492
BfmI CTRYAG 1 cut(s) 1397
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 538
BglII AGATCT 1 cut(s) 616
BisI GCNGC 3 cut(s) 152, 256, 1093
BlsI GCNGC 3 cut(s) 153, 257, 1094
BmcAI AGTACT 2 cut(s) 490, 925
Bme1390I CCNGG 3 cut(s) 369, 441, 1277
Bme18I GGWCC 3 cut(s) 933, 976, 1154
BmeT110I CYCGRG 2 cut(s) 484, 1149
BmgT120I GGNCC 3 cut(s) 933, 976, 1154
BmiI GGNNCC 3 cut(s) 646, 1155, 1436
BmrFI CCNGG 3 cut(s) 369, 441, 1277
BmrI ACTGGG 1 cut(s) 936
BmsI GCATC 7 cut(s) 260, 567, 644, 700, 764, 1090, 1466
BmuI ACTGGG 1 cut(s) 936
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 2 cut(s) 341, 1092
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 671, 1161
BpuMI CCSGG 2 cut(s) 369, 1277
Bsa29I ATCGAT 1 cut(s) 1408
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 4 cut(s) 64, 317, 579, 1438
BsaWI WCCGGW 2 cut(s) 145, 297
Bsc4I CCNNNNNNNGG 1 cut(s) 1421
Bse1I ACTGG 3 cut(s) 931, 994, 1109
Bse3DI GCAATG 4 cut(s) 280, 553, 750, 895
BseAI TCCGGA 2 cut(s) 145, 297
BseBI CCWGG 1 cut(s) 441
BseCI ATCGAT 1 cut(s) 1408
BseDI CCNNGG 4 cut(s) 64, 317, 579, 1438
BseGI GGATG 2 cut(s) 454, 755
BseLI CCNNNNNNNGG 1 cut(s) 1421
BseMI GCAATG 4 cut(s) 280, 553, 750, 895
BseMII CTCAG 3 cut(s) 27, 251, 627
BseNI ACTGG 3 cut(s) 931, 994, 1109
BseRI GAGGAG 4 cut(s) 36, 53, 62, 71
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 2 cut(s) 138, 1104
BsgI GTGCAG 1 cut(s) 592
BshFI GGCC 2 cut(s) 316, 1372
BshVI ATCGAT 1 cut(s) 1408
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 2 cut(s) 484, 1149
BsiSI CCGG 5 cut(s) 146, 298, 368, 1276, 1329
BslFI GGGAC 2 cut(s) 162, 1030
BslI CCNNNNNNNGG 1 cut(s) 1421
BsmAI GTCTC 2 cut(s) 240, 670
BsmFI GGGAC 2 cut(s) 162, 1030
BsmI GAATGC 3 cut(s) 136, 207, 555
BsnI GGCC 2 cut(s) 316, 1372
BsoBI CYCGRG 2 cut(s) 484, 1149
Bsp1286I GDGCHC 2 cut(s) 606, 614
Bsp13I TCCGGA 2 cut(s) 145, 297
Bsp143I GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
Bsp19I CCATGG 1 cut(s) 1438
BspACI CCGC 9 cut(s) 5, 18, 81, 92, 180, 256, 343, 505, 1069
BspANI GGCC 2 cut(s) 316, 1372
BspCNI CTCAG 3 cut(s) 28, 250, 626
BspDI ATCGAT 1 cut(s) 1408
BspEI TCCGGA 2 cut(s) 145, 297
BspLI GGNNCC 3 cut(s) 646, 1155, 1436
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 4 cut(s) 20, 137, 185, 665
BsrBI CCGCTC 1 cut(s) 1071
BsrDI GCAATG 4 cut(s) 280, 553, 750, 895
BsrI ACTGG 3 cut(s) 931, 994, 1109
BssECI CCNNGG 4 cut(s) 64, 317, 579, 1438
BssMI GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 1438
Bst2UI CCWGG 1 cut(s) 441
Bst6I CTCTTC 3 cut(s) 48, 1239, 1487
BstACI GRCGYC 1 cut(s) 423
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 4 cut(s) 164, 509, 624, 1097
BstDEI CTNAG 3 cut(s) 36, 237, 613
BstDSI CCRYGG 2 cut(s) 64, 1438
BstENI CCTNNNNNAGG 1 cut(s) 1419
BstF5I GGATG 2 cut(s) 454, 755
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 1210
BstKTI GATC 8 cut(s) 28, 145, 193, 568, 619, 673, 999, 1499
BstMAI GTCTC 2 cut(s) 240, 670
BstMBI GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
BstMWI GCNNNNNNNGC 4 cut(s) 41, 78, 213, 632
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 2 cut(s) 1173, 1386
BstSCI CCNGG 3 cut(s) 367, 439, 1275
BstSFI CTRYAG 1 cut(s) 1397
BstSLI GKGCMC 1 cut(s) 606
BstV1I GCAGC 2 cut(s) 138, 1104
BstX2I RGATCY 3 cut(s) 25, 142, 616
BstYI RGATCY 3 cut(s) 25, 142, 616
Bsu15I ATCGAT 1 cut(s) 1408
BsuRI GGCC 2 cut(s) 316, 1372
BsuTUI ATCGAT 1 cut(s) 1408
BtgI CCRYGG 2 cut(s) 64, 1438
BtgZI GCGATG 1 cut(s) 397
BtsCI GGATG 2 cut(s) 454, 755
BtsIMutI CAGTG 5 cut(s) 382, 575, 660, 987, 1207
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 4 cut(s) 164, 509, 624, 1097
CfoI GCGC 2 cut(s) 74, 1210
Cfr13I GGNCC 3 cut(s) 933, 976, 1154
ClaI ATCGAT 1 cut(s) 1408
CseI GACGC 1 cut(s) 500
Csp6I GTAC 6 cut(s) 489, 748, 924, 1126, 1173, 1476
CviAII CATG 5 cut(s) 607, 980, 1170, 1383, 1439
CviQI GTAC 6 cut(s) 489, 748, 924, 1126, 1173, 1476
DdeI CTNAG 3 cut(s) 36, 237, 613
DpnI GATC 8 cut(s) 27, 144, 192, 567, 618, 672, 998, 1498
DpnII GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
EaeI YGGCCR 1 cut(s) 1370
Eam1104I CTCTTC 3 cut(s) 48, 1239, 1487
EarI CTCTTC 3 cut(s) 48, 1239, 1487
EciI GGCGGA 2 cut(s) 20, 33
Ecl136II GAGCTC 1 cut(s) 612
Eco130I CCWWGG 1 cut(s) 1438
Eco24I GRGCYC 1 cut(s) 614
Eco47I GGWCC 3 cut(s) 933, 976, 1154
Eco53kI GAGCTC 1 cut(s) 612
Eco57I CTGAAG 1 cut(s) 1183
Eco88I CYCGRG 2 cut(s) 484, 1149
EcoICRI GAGCTC 1 cut(s) 612
EcoNI CCTNNNNNAGG 1 cut(s) 1419
EcoO109I RGGNCCY 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 1438
EcoT22I ATGCAT 2 cut(s) 555, 812
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 1438
FaeI CATG 5 cut(s) 610, 983, 1173, 1386, 1442
FalI AAGNNNNNCTT 2 cut(s) 1177, 1209
FaqI GGGAC 2 cut(s) 162, 1030
FatI CATG 5 cut(s) 606, 979, 1169, 1382, 1438
FauI CCCGC 2 cut(s) 187, 1076
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 2 cut(s) 514, 1350
Fnu4HI GCNGC 3 cut(s) 152, 256, 1093
FokI GGATG 2 cut(s) 461, 742
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 3 cut(s) 152, 256, 1093
FspBI CTAG 2 cut(s) 260, 492
GlaI GCGC 2 cut(s) 73, 1209
GluI GCNGC 3 cut(s) 152, 256, 1093
GsuI CTGGAG 2 cut(s) 341, 1092
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 316, 1372
HapII CCGG 5 cut(s) 146, 298, 368, 1276, 1329
HgaI GACGC 1 cut(s) 500
HhaI GCGC 2 cut(s) 74, 1210
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 5 cut(s) 610, 983, 1173, 1386, 1442
Hin6I GCGC 2 cut(s) 72, 1208
HinP1I GCGC 2 cut(s) 72, 1208
HincII GTYRAC 2 cut(s) 458, 515
HindII GTYRAC 2 cut(s) 458, 515
HinfI GANTC 8 cut(s) 185, 346, 364, 388, 400, 436, 1231, 1340
HpaII CCGG 5 cut(s) 146, 298, 368, 1276, 1329
HphI GGTGA 3 cut(s) 81, 533, 848
Hpy166II GTNNAC 6 cut(s) 404, 458, 515, 524, 1351, 1390
Hpy188III TCNNGA 6 cut(s) 146, 194, 298, 358, 427, 1178
Hpy8I GTNNAC 6 cut(s) 404, 458, 515, 524, 1351, 1390
Hpy99I CGWCG 5 cut(s) 113, 116, 216, 428, 516
HpyAV CCTTC 4 cut(s) 227, 325, 471, 687
HpyCH4IV ACGT 2 cut(s) 288, 423
HpyCH4V TGCA 9 cut(s) 273, 533, 553, 558, 573, 660, 755, 810, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 78, 213, 632
HpyF3I CTNAG 3 cut(s) 36, 237, 613
HpySE526I ACGT 2 cut(s) 288, 423
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 5 cut(s) 610, 983, 1173, 1386, 1442
HspAI GCGC 2 cut(s) 72, 1208
Kpn2I TCCGGA 2 cut(s) 145, 297
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
LmnI GCTCC 3 cut(s) 148, 171, 632
Lsp1109I GCAGC 2 cut(s) 138, 1104
LweI GCATC 7 cut(s) 260, 567, 644, 700, 764, 1090, 1466
MaeI CTAG 2 cut(s) 260, 492
MaeII ACGT 2 cut(s) 288, 423
MaeIII GTNAC 6 cut(s) 419, 583, 701, 715, 964, 1111
MalI GATC 8 cut(s) 27, 144, 192, 567, 618, 672, 998, 1498
MbiI CCGCTC 1 cut(s) 1071
MboI GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
MflI RGATCY 3 cut(s) 25, 142, 616
MhlI GDGCHC 2 cut(s) 606, 614
MluCI AATT 8 cut(s) 301, 322, 681, 903, 916, 1045, 1250, 1447
MlyI GAGTC 6 cut(s) 179, 340, 358, 409, 430, 1225
MmeI TCCRAC 1 cut(s) 193
Mph1103I ATGCAT 2 cut(s) 555, 812
MroI TCCGGA 2 cut(s) 145, 297
MroXI GAANNNNTTC 1 cut(s) 1135
MseI TTAA 3 cut(s) 1253, 1364, 1510
MslI CAYNNNNRTG 5 cut(s) 415, 696, 841, 1118, 1383
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 5 cut(s) 146, 298, 368, 1276, 1329
MspR9I CCNGG 3 cut(s) 369, 441, 1277
Mva1269I GAATGC 3 cut(s) 136, 207, 555
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 4 cut(s) 41, 78, 213, 632
NciI CCSGG 2 cut(s) 369, 1277
NcoI CCATGG 1 cut(s) 1438
NdeII GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
NlaIII CATG 5 cut(s) 610, 983, 1173, 1386, 1442
NlaIV GGNNCC 3 cut(s) 646, 1155, 1436
NmeAIII GCCGAG 2 cut(s) 342, 1348
NmuCI GTSAC 3 cut(s) 419, 701, 1111
NsiI ATGCAT 2 cut(s) 555, 812
NspI RCATGY 2 cut(s) 1173, 1386
OliI CACNNNNGTG 3 cut(s) 415, 841, 1118
PaeR7I CTCGAG 1 cut(s) 484
PciI ACATGT 2 cut(s) 1169, 1382
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 3 cut(s) 136, 207, 555
PdmI GAANNNNTTC 1 cut(s) 1135
PfeI GAWTC 2 cut(s) 388, 1340
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 3 cut(s) 153, 257, 1094
PleI GAGTC 6 cut(s) 179, 340, 358, 408, 430, 1225
PpsI GAGTC 6 cut(s) 179, 340, 358, 408, 430, 1225
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 1154
PscI ACATGT 2 cut(s) 1169, 1382
Psp124BI GAGCTC 1 cut(s) 614
Psp5II RGGWCCY 1 cut(s) 1154
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 3 cut(s) 646, 1155, 1436
PspPI GGNCC 3 cut(s) 933, 976, 1154
PspPPI RGGWCCY 1 cut(s) 1154
PspXI VCTCGAGB 1 cut(s) 484
PsuI RGATCY 3 cut(s) 25, 142, 616
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 6 cut(s) 490, 749, 925, 1127, 1174, 1477
RsaNI GTAC 6 cut(s) 489, 748, 924, 1126, 1173, 1476
RseI CAYNNNNRTG 5 cut(s) 415, 696, 841, 1118, 1383
SacI GAGCTC 1 cut(s) 614
SalI GTCGAC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 1253, 1364, 1510
SatI GCNGC 3 cut(s) 152, 256, 1093
Sau3AI GATC 8 cut(s) 25, 142, 190, 565, 616, 670, 996, 1496
Sau96I GGNCC 3 cut(s) 933, 976, 1154
ScaI AGTACT 2 cut(s) 490, 925
SchI GAGTC 6 cut(s) 179, 340, 358, 409, 430, 1225
ScrFI CCNGG 3 cut(s) 369, 441, 1277
SduI GDGCHC 2 cut(s) 606, 614
SfaNI GCATC 7 cut(s) 260, 567, 644, 700, 764, 1090, 1466
SfcI CTRYAG 1 cut(s) 1397
Sfr274I CTCGAG 1 cut(s) 484
SinI GGWCC 3 cut(s) 933, 976, 1154
SlaI CTCGAG 1 cut(s) 484
SmiMI CAYNNNNRTG 5 cut(s) 415, 696, 841, 1118, 1383
SmlI CTYRAG 3 cut(s) 484, 686, 1176
SmoI CTYRAG 3 cut(s) 484, 686, 1176
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 8 cut(s) 301, 322, 681, 903, 916, 1045, 1250, 1447
SsiI CCGC 9 cut(s) 5, 18, 81, 92, 180, 256, 343, 505, 1069
SspMI CTAG 2 cut(s) 260, 492
SstI GAGCTC 1 cut(s) 614
StyD4I CCNGG 3 cut(s) 367, 439, 1275
StyI CCWWGG 1 cut(s) 1438
TaiI ACGT 2 cut(s) 291, 426
TaqI TCGA 5 cut(s) 426, 485, 514, 1084, 1408
TasI AATT 8 cut(s) 301, 322, 681, 903, 916, 1045, 1250, 1447
TatI WGTACW 4 cut(s) 488, 747, 923, 1172
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 2 cut(s) 388, 1340
Tru1I TTAA 3 cut(s) 1253, 1364, 1510
Tru9I TTAA 3 cut(s) 1253, 1364, 1510
TscAI CASTG 5 cut(s) 382, 575, 667, 994, 1207
TseFI GTSAC 3 cut(s) 419, 701, 1111
TseI GCWGC 2 cut(s) 151, 1092
Tsp45I GTSAC 3 cut(s) 419, 701, 1111
TspDTI ATGAA 3 cut(s) 453, 929, 1058
TspRI CASTG 5 cut(s) 382, 575, 667, 994, 1207
VpaK11BI GGWCC 3 cut(s) 933, 976, 1154
XagI CCTNNNNNAGG 1 cut(s) 1419
XapI RAATTY 2 cut(s) 1045, 1447
XceI RCATGY 2 cut(s) 1173, 1386
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 484
XmiI GTMKAC 2 cut(s) 514, 1350
XmnI GAANNNNTTC 1 cut(s) 1135
XspI CTAG 2 cut(s) 260, 492
ZraI GACGTC 1 cut(s) 424
ZrmI AGTACT 2 cut(s) 490, 925
Zsp2I ATGCAT 2 cut(s) 555, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.