Rh6DG132800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002209
Physical Location & Seq
Forward (+)
18899673 .. 18916533
16861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG132800.1

Sequence Viewer

Length: 960 bp
ATGGACCATGCTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGATGTGCATAGTGGCAGTCAACCTTCATCTCGTACAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCAATGGGGTGAACAGCCTGCATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGGATGGATTGACTGGTATTACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTTTTCAATTTCAGTACTGGGAGGACCATTCATAAAACATCATTTAACAGTCAAGTTACTGCTATGGACCATGGTCACACTGGACAGATCATTTTCTGTGGAGATGGGACGGGGTGTATATATTCTGTCAGTATGAATTCTCTCACAGGAGTATTATCCCGTTCTCATCGGCATCGAAGTAGCAGCAGGCAGAAATCTCCAGTCACAAATGTGGAGTACCGAAGTTTTTTTCTGTTGGCTCGGGCCCTTGTTGCGCTGGAAATACAAGGTTATTTGACTCTTCGTTGCTCACTCAAATTAACTCCACGAATATACAATATCCGGGCTTCCTTTTGTCCTATGCTTTCCCTTGAAAAAGGAGAATATATAGTTGCCGGAAGTGAAGATTCAAGTGTAAACTTCTACGACTTAACTCGGCCAAAGCATACATGTGTAAACAAGCTACAGTCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

319

Amino Acids

35.41

Weight (kDa)

8.56

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 38 - 168 1.7e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 59 - 157 1.2e-10 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 64 - 241 4.6e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 65 - 156 1.3e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 66 - 172 2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 67 - 308 2.7e-13 WDR5 beta-propeller domain
WD40_Prp19 PF24814 69 - 159 1.9e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 70 - 158 1.4e-07 THOC3 beta-propeller domain
WD40 PF00400 104 - 144 7.7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 130
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 920
AcsI RAATTY 1 cut(s) 640
AfaI GTAC 5 cut(s) 97, 115, 392, 520, 722
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 6 cut(s) 220, 306, 441, 511, 857, 894
AhlI ACTAGT 1 cut(s) 116
AleI CACNNNNGTG 2 cut(s) 484, 713
AluBI AGCT 2 cut(s) 237, 946
AluI AGCT 2 cut(s) 237, 946
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 2 cut(s) 109, 744
AoxI GGCC 3 cut(s) 445, 747, 920
ApaI GGGCCC 1 cut(s) 751
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 1 cut(s) 640
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 5 cut(s) 4, 528, 571, 747, 748
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 2 cut(s) 158, 491
AvaI CYCGRG 2 cut(s) 109, 744
AvaII GGWCC 3 cut(s) 4, 528, 571
BaeGI GKGCMC 2 cut(s) 231, 751
BanII GRGCYC 2 cut(s) 239, 751
Bbv12I GWGCWC 1 cut(s) 239
BbvI GCAGC 1 cut(s) 699
BccI CCATC 4 cut(s) 35, 260, 342, 602
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 1 cut(s) 827
BcoDI GTCTC 1 cut(s) 295
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 947
BglII AGATCT 1 cut(s) 241
BisI GCNGC 1 cut(s) 688
BlsI GCNGC 1 cut(s) 689
BmcAI AGTACT 2 cut(s) 115, 520
Bme1390I CCNGG 1 cut(s) 827
Bme18I GGWCC 3 cut(s) 4, 528, 571
BmeT110I CYCGRG 2 cut(s) 109, 744
BmgT120I GGNCC 5 cut(s) 4, 528, 571, 747, 748
BmiI GGNNCC 2 cut(s) 271, 749
BmrFI CCNGG 1 cut(s) 827
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 5 cut(s) 192, 269, 325, 407, 685
BmuI ACTGGG 1 cut(s) 531
BoxI GACNNNNGTC 1 cut(s) 576
BpmI CTGGAG 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 827
BsaJI CCNNGG 2 cut(s) 204, 574
Bse1I ACTGG 5 cut(s) 22, 362, 526, 589, 704
Bse3DI GCAATG 2 cut(s) 178, 393
BseDI CCNNGG 2 cut(s) 204, 574
BseGI GGATG 2 cut(s) 353, 398
BseMI GCAATG 2 cut(s) 178, 393
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 5 cut(s) 22, 362, 526, 589, 704
BseSI GKGCMC 2 cut(s) 231, 751
BseXI GCAGC 1 cut(s) 699
BsgI GTGCAG 1 cut(s) 217
BshFI GGCC 3 cut(s) 447, 749, 922
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 2 cut(s) 109, 744
BsiSI CCGG 2 cut(s) 826, 879
BslFI GGGAC 2 cut(s) 58, 625
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 2 cut(s) 58, 625
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 3 cut(s) 447, 749, 922
BsoBI CYCGRG 2 cut(s) 109, 744
Bsp120I GGGCCC 1 cut(s) 747
Bsp1286I GDGCHC 3 cut(s) 231, 239, 751
Bsp143I GATC 5 cut(s) 24, 190, 241, 295, 591
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 1 cut(s) 130
BspANI GGCC 3 cut(s) 447, 749, 922
BspCNI CTCAG 1 cut(s) 251
BspLI GGNNCC 2 cut(s) 271, 749
BspPI GGATC 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 178, 393
BsrI ACTGG 5 cut(s) 22, 362, 526, 589, 704
BssECI CCNNGG 2 cut(s) 204, 574
BssMI GATC 5 cut(s) 24, 190, 241, 295, 591
BssT1I CCWWGG 1 cut(s) 574
Bst4CI ACNGT 7 cut(s) 214, 280, 289, 421, 486, 554, 951
Bst6I CTCTTC 1 cut(s) 789
BstC8I GCNNGC 4 cut(s) 134, 156, 249, 692
BstDEI CTNAG 1 cut(s) 238
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 2 cut(s) 353, 398
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 5 cut(s) 27, 193, 244, 298, 594
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 5 cut(s) 24, 190, 241, 295, 591
BstMWI GCNNNNNNNGC 2 cut(s) 257, 755
BstNSI RCATGY 1 cut(s) 936
BstPAI GACNNNNGTC 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 825
BstSFI CTRYAG 1 cut(s) 947
BstSLI GKGCMC 2 cut(s) 231, 751
BstV1I GCAGC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 3 cut(s) 447, 749, 922
BtgI CCRYGG 1 cut(s) 574
BtsCI GGATG 2 cut(s) 353, 398
BtsIMutI CAGTG 5 cut(s) 15, 200, 285, 582, 960
Cac8I GCNNGC 4 cut(s) 134, 156, 249, 692
CfoI GCGC 1 cut(s) 760
Cfr13I GGNCC 5 cut(s) 4, 528, 571, 747, 748
CseI GACGC 1 cut(s) 125
Csp6I GTAC 5 cut(s) 96, 114, 391, 519, 721
CviAII CATG 4 cut(s) 8, 232, 575, 933
CviQI GTAC 5 cut(s) 96, 114, 391, 519, 721
DdeI CTNAG 1 cut(s) 238
DpnI GATC 5 cut(s) 26, 192, 243, 297, 593
DpnII GATC 5 cut(s) 24, 190, 241, 295, 591
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 239, 751
Eco47I GGWCC 3 cut(s) 4, 528, 571
Eco53kI GAGCTC 1 cut(s) 237
Eco88I CYCGRG 2 cut(s) 109, 744
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 574
EcoT22I ATGCAT 2 cut(s) 180, 455
EcoT38I GRGCYC 2 cut(s) 239, 751
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 4 cut(s) 11, 235, 578, 936
FaqI GGGAC 2 cut(s) 58, 625
FatI CATG 4 cut(s) 7, 231, 574, 932
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 688
FokI GGATG 2 cut(s) 360, 385
FriOI GRGCYC 2 cut(s) 239, 751
Fsp4HI GCNGC 1 cut(s) 688
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 759
GluI GCNGC 1 cut(s) 688
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 3 cut(s) 447, 749, 922
HapII CCGG 2 cut(s) 826, 879
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 760
Hin1II CATG 4 cut(s) 11, 235, 578, 936
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HincII GTYRAC 1 cut(s) 83
HindII GTYRAC 1 cut(s) 83
HinfI GANTC 2 cut(s) 781, 890
HpaII CCGG 2 cut(s) 826, 879
HphI GGTGA 2 cut(s) 158, 491
Hpy166II GTNNAC 4 cut(s) 83, 149, 901, 940
Hpy188I TCNGA 5 cut(s) 64, 241, 265, 295, 321
Hpy8I GTNNAC 4 cut(s) 83, 149, 901, 940
HpyAV CCTTC 2 cut(s) 96, 312
HpyCH4III ACNGT 7 cut(s) 214, 280, 289, 421, 486, 554, 951
HpyCH4V TGCA 8 cut(s) 70, 158, 178, 183, 198, 285, 398, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 755
HpyF3I CTNAG 1 cut(s) 238
Hsp92II CATG 4 cut(s) 11, 235, 578, 936
HspAI GCGC 1 cut(s) 758
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 5 cut(s) 24, 190, 241, 295, 591
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 699
LweI GCATC 5 cut(s) 192, 269, 325, 407, 685
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 6 cut(s) 208, 326, 340, 559, 578, 706
MalI GATC 5 cut(s) 26, 192, 243, 297, 593
MboI GATC 5 cut(s) 24, 190, 241, 295, 591
MboII GAAGA 2 cut(s) 776, 899
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 3 cut(s) 231, 239, 751
MluCI AATT 4 cut(s) 306, 511, 640, 800
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 4 cut(s) 214, 309, 412, 519
Mph1103I ATGCAT 2 cut(s) 180, 455
MseI TTAA 3 cut(s) 549, 803, 914
MslI CAYNNNNRTG 4 cut(s) 321, 484, 713, 933
MspI CCGG 2 cut(s) 826, 879
MspR9I CCNGG 1 cut(s) 827
Mva1269I GAATGC 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 257, 755
NciI CCSGG 1 cut(s) 827
NcoI CCATGG 1 cut(s) 574
NdeII GATC 5 cut(s) 24, 190, 241, 295, 591
NlaIII CATG 4 cut(s) 11, 235, 578, 936
NlaIV GGNNCC 2 cut(s) 271, 749
NmeAIII GCCGAG 1 cut(s) 898
NmuCI GTSAC 3 cut(s) 326, 578, 706
NsiI ATGCAT 2 cut(s) 180, 455
NspI RCATGY 1 cut(s) 936
OliI CACNNNNGTG 2 cut(s) 484, 713
PaeR7I CTCGAG 1 cut(s) 109
PceI AGGCCT 1 cut(s) 447
PciI ACATGT 1 cut(s) 932
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 890
PkrI GCNGC 1 cut(s) 689
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
PscI ACATGT 1 cut(s) 932
PshAI GACNNNNGTC 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 271, 749
PspOMI GGGCCC 1 cut(s) 747
PspPI GGNCC 5 cut(s) 4, 528, 571, 747, 748
PspXI VCTCGAGB 1 cut(s) 109
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 5 cut(s) 97, 115, 392, 520, 722
RsaNI GTAC 5 cut(s) 96, 114, 391, 519, 721
RseI CAYNNNNRTG 4 cut(s) 321, 484, 713, 933
SacI GAGCTC 1 cut(s) 239
SaqAI TTAA 3 cut(s) 549, 803, 914
SatI GCNGC 1 cut(s) 688
Sau3AI GATC 5 cut(s) 24, 190, 241, 295, 591
Sau96I GGNCC 5 cut(s) 4, 528, 571, 747, 748
ScaI AGTACT 2 cut(s) 115, 520
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 1 cut(s) 827
SduI GDGCHC 3 cut(s) 231, 239, 751
SetI ASST 5 cut(s) 88, 239, 275, 775, 948
SfaNI GCATC 5 cut(s) 192, 269, 325, 407, 685
SfcI CTRYAG 1 cut(s) 947
Sfr274I CTCGAG 1 cut(s) 109
SinI GGWCC 3 cut(s) 4, 528, 571
SlaI CTCGAG 1 cut(s) 109
SmiMI CAYNNNNRTG 4 cut(s) 321, 484, 713, 933
SmlI CTYRAG 2 cut(s) 109, 311
SmoI CTYRAG 2 cut(s) 109, 311
SpeI ACTAGT 1 cut(s) 116
Sse9I AATT 4 cut(s) 306, 511, 640, 800
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 130
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 239
StuI AGGCCT 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 825
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 7 cut(s) 214, 280, 289, 421, 486, 554, 951
TaqI TCGA 2 cut(s) 110, 679
TasI AATT 4 cut(s) 306, 511, 640, 800
TatI WGTACW 3 cut(s) 113, 390, 518
TfiI GAWTC 1 cut(s) 890
Tru1I TTAA 3 cut(s) 549, 803, 914
Tru9I TTAA 3 cut(s) 549, 803, 914
TscAI CASTG 5 cut(s) 22, 200, 292, 589, 960
TseFI GTSAC 3 cut(s) 326, 578, 706
TseI GCWGC 1 cut(s) 687
Tsp45I GTSAC 3 cut(s) 326, 578, 706
TspDTI ATGAA 3 cut(s) 78, 524, 653
TspRI CASTG 5 cut(s) 22, 200, 292, 589, 960
VpaK11BI GGWCC 3 cut(s) 4, 528, 571
XapI RAATTY 1 cut(s) 640
XceI RCATGY 1 cut(s) 936
XcmI CCANNNNNNNNNTGG 2 cut(s) 14, 581
XhoI CTCGAG 1 cut(s) 109
XspI CTAG 1 cut(s) 117
ZrmI AGTACT 2 cut(s) 115, 520
Zsp2I ATGCAT 2 cut(s) 180, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.