AT2G44130

kelch repeat-containing F-box family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003364
Physical Location & Seq
Forward (+)
18253610 .. 18255054
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44130.1

Sequence Viewer

Length: 1230 bp
ATGACTATGGAAGTGTCGAAAAAGAAAGGTGGAGATTTTCAGCAATGTCACGAATTGATTCCCGGTTTACCTTCCGAACTAGCTTTGGAGTGTTTGGTCAGAGTTCCGTTCCAATTCCAATCCGCCATGAGATCCGTTTGCCGTTCTTGGCGTAGCTTACTCTCCGACTCTTCTTTTATCCAAGAACGGCGGAGATGCGGCAAAACAGAGCTTCTCCTCTGCCTCGTTCAACCGCTAACGCCGCCAATTCCAGCGTCTAAATCGGTTGACGAGACATTAATGGTTGACGAGAAAAAATCAGAGGATGAGTCACACCCGCGCGTTTTCTGCACGCCGCGTTTTGGATTGAGCGTTTACAACGCTGCGATGTCCACGTGGCATCGCGTTGCGTTTCCAGAGGAGGAGCAGATCCCGCTTTTCTGCGAGTGCGTCGTGCTTCAGGACGCCGGGAAGATTCTGCTCATCGGCGGTTGGGATCCGGAGACGTTACAGCCGACGAGAGACGTTTACGTTCTGGAATTCGCCGGAAGGAAGTGGAGACGAGGTGCGCCAATGAAGGAATCACGATCATTCTTCGCCTGCGCCTCCGTAAGTCCAACGAAGGTATACGTCGCCGGAGGTCACGACGATCAGAAAAACGCTTTACGCTCGGCGGAGGTGTACGACGTCGAGAAAGACGAGTGGTCGTCGGTTACTCCGATGACGGAAGGAAGAGACGAATGTCAAGGATTCGCCGTTGGTATGGGTCTAAGGTTTTGCGTGCTGAGCGGTTACGGAACGGAATCTCAAGGAAGATTCCGATCTGACGGAGAAATTTACGATCCAGCGACAGATTCATGGTCGAGAATCGACAACGTCTGGCGATTTCCAGATACTAGCCCGAGAGGTCGCACCGCCGGAGACTTTAGAAGCTCCTCTACGTTATGGTGTTTCACAGACACTGATTTACAGAGTGAACGTCGATGGGAAACTAACGATGATTCGAGAAATTTGAAACTGGATCTTCAATCTATACAGCTTCCGATGACAGGAAGCTCAGTTTTCGCCGGAAGCTTAGGAGGTGAATCGGTGGTAATGATTGGCGGTAAAAGAGAGAGTGAAGGTGAAGGTGAAGGAGGAGTGATGATGAAGATGACGACTGAGAAGAAAATGGGAAAATGGAGTCATCATGTTCATATACCTTGTGATTTCTCTACTCTTCCATTTTCGCATGCTTCAATCTATGTTTGA

Protein Analysis

409

Amino Acids

45.89

Weight (kDa)

5.54

Isoelectric Point (pI)

63.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 59 2.3e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 116 - 286 2.3e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 118 - 293 1.4e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 131 - 287 6.5e-11 FKB95, Kelch-repeats domain
Kelch_1 PF01344 141 - 186 2.3e-06 Kelch motif
Beta-prop_TYW4 PF13418 146 - 239 9.2e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_Calicin PF13964 152 - 283 3.3e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 192 - 235 4.5e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 669
AccBSI CCGCTC 1 cut(s) 768
AccI GTMKAC 1 cut(s) 606
AccII CGCG 4 cut(s) 319, 321, 337, 384
AccIII TCCGGA 1 cut(s) 478
AclWI GGATC 6 cut(s) 126, 403, 470, 483, 815, 1008
AcsI RAATTY 3 cut(s) 518, 813, 988
AcuI CTGAAG 1 cut(s) 422
AcvI CACGTG 1 cut(s) 375
AcyI GRCGYC 2 cut(s) 444, 666
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 2 cut(s) 341, 1028
AgsI TTSAA 4 cut(s) 230, 994, 1007, 1218
AhdI GACNNNNNGTC 1 cut(s) 682
AluBI AGCT 7 cut(s) 83, 156, 211, 912, 1018, 1035, 1053
AluI AGCT 7 cut(s) 83, 156, 211, 912, 1018, 1035, 1053
Alw26I GTCTC 6 cut(s) 266, 476, 495, 532, 708, 894
AlwI GGATC 6 cut(s) 126, 403, 470, 483, 815, 1008
AlwNI CAGNNNCTG 1 cut(s) 941
Ama87I CYCGRG 1 cut(s) 880
Aor13HI TCCGGA 1 cut(s) 478
ApeKI GCWGC 1 cut(s) 362
ApoI RAATTY 3 cut(s) 518, 813, 988
AseI ATTAAT 1 cut(s) 278
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 3 cut(s) 321, 550, 584
AsuC2I CCSGG 2 cut(s) 63, 448
AsuHPI GGTGA 3 cut(s) 1073, 1115, 1121
AvaI CYCGRG 1 cut(s) 880
BamHI GGATCC 1 cut(s) 475
BarI GAAGNNNNNNTAC 2 cut(s) 901, 933
BbrPI CACGTG 1 cut(s) 375
BbvI GCAGC 1 cut(s) 349
BccI CCATC 1 cut(s) 957
BceAI ACGGC 3 cut(s) 126, 203, 719
BcnI CCSGG 2 cut(s) 63, 448
BcoDI GTCTC 6 cut(s) 266, 476, 495, 532, 708, 894
BfaI CTAG 2 cut(s) 80, 876
BisI GCNGC 4 cut(s) 199, 242, 335, 363
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 4 cut(s) 200, 243, 336, 364
Bme1390I CCNGG 2 cut(s) 63, 448
BmeRI GACNNNNNGTC 1 cut(s) 682
BmeT110I CYCGRG 1 cut(s) 880
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 2 cut(s) 63, 448
BmsI GCATC 2 cut(s) 185, 388
BoxI GACNNNNGTC 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 1054
Bpu1102I GCTNAGC 1 cut(s) 764
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 63, 448
BsaAI YACGTR 1 cut(s) 375
BsaBI GATNNNNATC 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 444, 666
BsaWI WCCGGW 1 cut(s) 478
BsaXI ACNNNNNCTCC 2 cut(s) 529, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 1028
Bse1I ACTGG 1 cut(s) 1002
Bse3DI GCAATG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 799
BseAI TCCGGA 1 cut(s) 478
BseGI GGATG 1 cut(s) 310
BseJI GATNNNNATC 1 cut(s) 799
BseLI CCNNNNNNNGG 2 cut(s) 341, 1028
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 3 cut(s) 755, 1050, 1131
BseNI ACTGG 1 cut(s) 1002
BseRI GAGGAG 5 cut(s) 206, 413, 416, 904, 1131
BseXI GCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 313
Bsh1236I CGCG 4 cut(s) 319, 321, 337, 384
BsiHKCI CYCGRG 1 cut(s) 880
BsiSI CCGG 7 cut(s) 63, 447, 479, 525, 615, 897, 1047
BslI CCNNNNNNNGG 2 cut(s) 341, 1028
BsmAI GTCTC 6 cut(s) 266, 476, 495, 532, 708, 894
BsmBI CGTCTC 4 cut(s) 476, 495, 532, 708
BsoBI CYCGRG 1 cut(s) 880
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
Bsp1720I GCTNAGC 1 cut(s) 764
BspCNI CTCAG 3 cut(s) 756, 1049, 1132
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 4 cut(s) 319, 321, 337, 384
BspLI GGNNCC 1 cut(s) 477
BspPI GGATC 6 cut(s) 126, 403, 470, 483, 815, 1008
BsrBI CCGCTC 1 cut(s) 768
BsrDI GCAATG 1 cut(s) 50
BsrI ACTGG 1 cut(s) 1002
BssMI GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
BssNAI GTATAC 1 cut(s) 607
BssNI GRCGYC 2 cut(s) 444, 666
Bst1107I GTATAC 1 cut(s) 607
Bst6I CTCTTC 3 cut(s) 175, 706, 1203
BstACI GRCGYC 2 cut(s) 444, 666
BstBAI YACGTR 1 cut(s) 375
BstC8I GCNNGC 4 cut(s) 332, 580, 761, 1212
BstDEI CTNAG 5 cut(s) 749, 764, 1036, 1054, 1140
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 4 cut(s) 319, 321, 337, 384
BstHHI GCGC 3 cut(s) 321, 550, 584
BstKTI GATC 8 cut(s) 134, 411, 478, 569, 631, 803, 823, 1003
BstMAI GTCTC 6 cut(s) 266, 476, 495, 532, 708, 894
BstMBI GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
BstMWI GCNNNNNNNGC 4 cut(s) 241, 327, 412, 765
BstNSI RCATGY 1 cut(s) 1214
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 2 cut(s) 61, 446
BstUI CGCG 4 cut(s) 319, 321, 337, 384
BstV1I GCAGC 1 cut(s) 349
BstX2I RGATCY 4 cut(s) 131, 408, 475, 1000
BstYI RGATCY 4 cut(s) 131, 408, 475, 1000
BstZ17I GTATAC 1 cut(s) 607
BtgZI GCGATG 2 cut(s) 365, 380
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 939
Cac8I GCNNGC 4 cut(s) 332, 580, 761, 1212
CaiI CAGNNNCTG 1 cut(s) 941
CfoI GCGC 3 cut(s) 321, 550, 584
CseI GACGC 3 cut(s) 243, 418, 452
Csp6I GTAC 1 cut(s) 661
CviAII CATG 4 cut(s) 127, 837, 1169, 1211
CviJI RGCY 9 cut(s) 83, 156, 211, 493, 879, 912, 1018, 1035, 1053
CviKI_1 RGCY 9 cut(s) 83, 156, 211, 493, 879, 912, 1018, 1035, 1053
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 5 cut(s) 749, 764, 1036, 1054, 1140
DpnI GATC 8 cut(s) 133, 410, 477, 568, 630, 802, 822, 1002
DpnII GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
DriI GACNNNNNGTC 1 cut(s) 682
Eam1104I CTCTTC 3 cut(s) 175, 706, 1203
Eam1105I GACNNNNNGTC 1 cut(s) 682
EarI CTCTTC 3 cut(s) 175, 706, 1203
EciI GGCGGA 3 cut(s) 112, 205, 668
Eco57I CTGAAG 1 cut(s) 422
Eco72I CACGTG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 880
EcoRI GAATTC 1 cut(s) 518
Esp3I CGTCTC 4 cut(s) 476, 495, 532, 708
FaeI CATG 4 cut(s) 130, 840, 1172, 1214
FatI CATG 4 cut(s) 126, 836, 1168, 1210
FauI CCCGC 2 cut(s) 324, 420
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 199, 242, 335, 363
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 4 cut(s) 199, 242, 335, 363
FspBI CTAG 2 cut(s) 80, 876
GlaI GCGC 3 cut(s) 320, 549, 583
GluI GCNGC 4 cut(s) 199, 242, 335, 363
HapII CCGG 7 cut(s) 63, 447, 479, 525, 615, 897, 1047
HgaI GACGC 3 cut(s) 243, 418, 452
HhaI GCGC 3 cut(s) 321, 550, 584
Hin1I GRCGYC 2 cut(s) 444, 666
Hin1II CATG 4 cut(s) 130, 840, 1172, 1214
Hin6I GCGC 3 cut(s) 319, 548, 582
HinP1I GCGC 3 cut(s) 319, 548, 582
HincII GTYRAC 2 cut(s) 268, 286
HindII GTYRAC 2 cut(s) 268, 286
HindIII AAGCTT 1 cut(s) 1051
HpaII CCGG 7 cut(s) 63, 447, 479, 525, 615, 897, 1047
HphI GGTGA 3 cut(s) 1073, 1115, 1121
Hpy166II GTNNAC 9 cut(s) 68, 268, 286, 355, 372, 508, 607, 661, 956
Hpy188I TCNGA 9 cut(s) 76, 101, 166, 301, 633, 699, 800, 805, 1023
Hpy8I GTNNAC 9 cut(s) 68, 268, 286, 355, 372, 508, 607, 661, 956
Hpy99I CGWCG 8 cut(s) 434, 499, 614, 629, 668, 671, 691, 963
HpyAV CCTTC 8 cut(s) 81, 522, 550, 595, 701, 1094, 1100, 1106
HpyCH4IV ACGT 9 cut(s) 374, 485, 504, 510, 609, 666, 855, 920, 958
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 327, 412, 765
HpyF3I CTNAG 5 cut(s) 749, 764, 1036, 1054, 1140
HpySE526I ACGT 9 cut(s) 374, 485, 504, 510, 609, 666, 855, 920, 958
Hsp92I GRCGYC 2 cut(s) 444, 666
Hsp92II CATG 4 cut(s) 130, 840, 1172, 1214
HspAI GCGC 3 cut(s) 319, 548, 582
Kpn2I TCCGGA 1 cut(s) 478
Kzo9I GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
LmnI GCTCC 2 cut(s) 403, 917
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 2 cut(s) 185, 388
MaeI CTAG 2 cut(s) 80, 876
MaeII ACGT 9 cut(s) 374, 485, 504, 510, 609, 666, 855, 920, 958
MaeIII GTNAC 6 cut(s) 47, 309, 486, 620, 691, 770
MalI GATC 8 cut(s) 133, 410, 477, 568, 630, 802, 822, 1002
MbiI CCGCTC 1 cut(s) 768
MboI GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
MboII GAAGA 9 cut(s) 162, 463, 565, 723, 804, 995, 1141, 1156, 1190
MflI RGATCY 4 cut(s) 131, 408, 475, 1000
MluCI AATT 6 cut(s) 53, 113, 246, 518, 813, 988
MlyI GAGTC 3 cut(s) 161, 317, 1171
MmeI TCCRAC 2 cut(s) 189, 620
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 1 cut(s) 278
MspI CCGG 7 cut(s) 63, 447, 479, 525, 615, 897, 1047
MspR9I CCNGG 2 cut(s) 63, 448
MvnI CGCG 4 cut(s) 319, 321, 337, 384
MwoI GCNNNNNNNGC 4 cut(s) 241, 327, 412, 765
NciI CCSGG 2 cut(s) 63, 448
NdeII GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
NlaIII CATG 4 cut(s) 130, 840, 1172, 1214
NlaIV GGNNCC 1 cut(s) 477
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 3 cut(s) 47, 309, 620
NspI RCATGY 1 cut(s) 1214
PaeI GCATGC 1 cut(s) 1214
PcsI WCGNNNNNNNCGW 3 cut(s) 491, 675, 695
PdmI GAANNNNTTC 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 854
PkrI GCNGC 4 cut(s) 200, 243, 336, 364
PleI GAGTC 3 cut(s) 161, 316, 1170
PmaCI CACGTG 1 cut(s) 375
PmlI CACGTG 1 cut(s) 375
PpsI GAGTC 3 cut(s) 161, 316, 1170
Ppu21I YACGTR 1 cut(s) 375
PshAI GACNNNNGTC 1 cut(s) 720
PshBI ATTAAT 1 cut(s) 278
PspCI CACGTG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 477
PstNI CAGNNNCTG 1 cut(s) 941
PsuI RGATCY 4 cut(s) 131, 408, 475, 1000
PsyI GACNNNGTC 1 cut(s) 854
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 4 cut(s) 199, 242, 335, 363
Sau3AI GATC 8 cut(s) 131, 408, 475, 566, 628, 800, 820, 1000
SchI GAGTC 3 cut(s) 161, 317, 1171
ScrFI CCNGG 2 cut(s) 63, 448
SfaNI GCATC 2 cut(s) 185, 388
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SphI GCATGC 1 cut(s) 1214
Sse9I AATT 6 cut(s) 53, 113, 246, 518, 813, 988
SspMI CTAG 2 cut(s) 80, 876
StyD4I CCNGG 2 cut(s) 61, 446
TaiI ACGT 9 cut(s) 377, 488, 507, 513, 612, 669, 858, 923, 961
TaqI TCGA 6 cut(s) 17, 669, 842, 849, 961, 983
TasI AATT 6 cut(s) 53, 113, 246, 518, 813, 988
TauI GCSGC 3 cut(s) 201, 244, 337
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TscAI CASTG 1 cut(s) 946
TseFI GTSAC 3 cut(s) 47, 309, 620
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 3 cut(s) 47, 309, 620
TspDTI ATGAA 4 cut(s) 569, 825, 1142, 1163
TspGWI ACGGA 7 cut(s) 96, 124, 577, 719, 789, 794, 822
TspRI CASTG 1 cut(s) 946
Tth111I GACNNNGTC 1 cut(s) 854
VspI ATTAAT 1 cut(s) 278
XapI RAATTY 3 cut(s) 518, 813, 988
XceI RCATGY 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 2 cut(s) 80, 876
ZraI GACGTC 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.