Rh5AG531400

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003364
Physical Location & Seq
Forward (+)
91346141 .. 91348602
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG531400.1

Sequence Viewer

Length: 1071 bp
ATGCGTAAAAATCAATTCTCGGCTGAAATGGGGATTCCTAAAGTCCCCGAGTGGATTCCGGGCCTGCCGGAAGAGCTCGGCCTCGAGTGCCTGACCCGGCTTCCGTACTCGGCCCACCCGACCGCCTCCCGGGTCTGCCGTCCGTGGCGGTCCTTGCTCGAGAGCGAGGAGTTCTATCGCCACAGGAAGCAGAACGGGTACACCCGAAACGTGGCGTGCTTGGTCCAGGCCGTTGCGGTTGCCGTCGAATTGAACGCGGGCCCCAAAGCGGCGGGTGCCGGAGGCTCGCCGAGTTACGGGATCGCCGTGTTCGACTCGGTGAGTGGGACCTGGGAGCGGCTCGACCCGATTCCCAAGTACCCGAATGGGCTGCCGATGTTTTGTCGGGTGGCGAGCTGCGAGGGCAAGCTGGTGGTGATGGGCGGGTGGGATCCGGCGACGTACAAGCCGGTCACCGACGTGTTCGTTTACGAGTTTACGACGTGTCGTTGGAGGAGGGGGGCGGCAATGCCGTCGAAGCGGTCGTTTTTCGCGATCGGGTCGCGCGGGAGTCGGGTTTATATTGCGGGCGGGCACGACGAGAGCAAGAACGCGCTGAAATCGGCGTGGGTTTACGATCTGAGGCGGGACGAGTGGGCCGAGTTGACTCAGATGAGTCGGGAGCGGGACGAGTGTCACGGGGTGGTGATCGGGGACGAGTTCTGGGTGGTGAGCGGGTACGGGACGGCGAGCCAGGGAGCGTTTGAGGGCGGAGCGGAGGCGTTGGATCTCGGGTCGGGTCGGTGGAGGCGGGTCGAGGGCGCGTGGGAGGAAGGGGAGTGTCCGAGGGCGTGTGGGGGTGGAGTTGGGAGAGATGGGAGGCTGGTGAGTTGGGCCGAGTTTGGCCCGGCGGTCCGGGTCGGGACCCGTGGGGTGAATCTCGGGTCCGGGGTCCTGGTGATGGGTTCGGAGTACGATGGCGGGAGCCAAGGGGTATTTATGAAGGATGGGCAAAATGGTAAATTAGAGAGGGTTAGCGTGCCTGAAGAGTTTTCTGGGTTTATTCAGTCAGGCTGTTGCGTTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

356

Amino Acids

38.8

Weight (kDa)

6.19

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 60 1.7e-07 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 101 - 268 6.9e-15 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 103 - 244 5e-10 FBX42, beta-propeller domain
Kelch_1 PF01344 128 - 171 1.3e-07 Kelch motif
Beta-prop_Calicin PF13964 138 - 223 2.8e-10 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 139 - 266 8.6e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 147 - 240 2.6e-07 FKB95, Kelch-repeats domain
Kelch_1 PF01344 174 - 219 8.3e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccBSI CCGCTC 4 cut(s) 337, 664, 714, 755
AccII CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
AclWI GGATC 4 cut(s) 308, 425, 438, 774
AcuI CTGAAG 1 cut(s) 1044
AdeI CACNNNGTG 1 cut(s) 682
AfaI GTAC 6 cut(s) 107, 200, 359, 443, 719, 953
AfiI CCNNNNNNNGG 6 cut(s) 129, 211, 268, 296, 336, 940
AflIII ACRYGT 2 cut(s) 459, 482
AgsI TTSAA 1 cut(s) 253
AjiI CACGTC 2 cut(s) 460, 483
AjnI CCWGG 4 cut(s) 225, 329, 732, 933
AleI CACNNNNGTG 1 cut(s) 458
AluBI AGCT 3 cut(s) 76, 396, 409
AluI AGCT 3 cut(s) 76, 396, 409
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 4 cut(s) 308, 425, 438, 774
Ama87I CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
AoxI GGCC 8 cut(s) 61, 79, 111, 228, 259, 636, 873, 883
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 370, 396
AspLEI GCGC 3 cut(s) 546, 595, 803
AsuC2I CCSGG 7 cut(s) 60, 97, 130, 131, 887, 896, 928
AsuHPI GGTGA 8 cut(s) 331, 427, 445, 697, 721, 877, 925, 949
AvaI CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
AvaII GGWCC 7 cut(s) 150, 223, 327, 892, 903, 924, 931
BaeGI GKGCMC 2 cut(s) 263, 576
BamHI GGATCC 1 cut(s) 430
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 78, 263
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 357, 383
BccI CCATC 5 cut(s) 412, 848, 934, 950, 980
BceAI ACGGC 6 cut(s) 123, 215, 227, 290, 496, 741
BcgI CGANNNNNNTGC 4 cut(s) 352, 386, 495, 529
BciT130I CCWGG 4 cut(s) 227, 331, 734, 935
BcnI CCSGG 7 cut(s) 60, 97, 130, 131, 887, 896, 928
BisI GCNGC 5 cut(s) 270, 338, 371, 397, 504
BlsI GCNGC 5 cut(s) 271, 339, 372, 398, 505
Bme18I GGWCC 7 cut(s) 150, 223, 327, 892, 903, 924, 931
BmeT110I CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
BmgBI CACGTC 2 cut(s) 460, 483
BoxI GACNNNNGTC 1 cut(s) 672
BpuMI CCSGG 7 cut(s) 60, 97, 130, 131, 887, 896, 928
BsaJI CCNNGG 8 cut(s) 129, 143, 330, 733, 824, 907, 927, 967
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 211, 268, 296, 336, 940
Bse118I RCCGGY 1 cut(s) 448
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 4 cut(s) 227, 331, 734, 935
BseDI CCNNGG 8 cut(s) 129, 143, 330, 733, 824, 907, 927, 967
BseGI GGATG 1 cut(s) 991
BseLI CCNNNNNNNGG 6 cut(s) 129, 211, 268, 296, 336, 940
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 2 cut(s) 611, 662
BseRI GAGGAG 2 cut(s) 182, 508
BseSI GKGCMC 2 cut(s) 263, 576
BseXI GCAGC 2 cut(s) 357, 383
Bsh1236I CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
Bsh1285I CGRYCG 3 cut(s) 123, 524, 537
BshFI GGCC 8 cut(s) 63, 81, 113, 230, 261, 638, 875, 885
BshNI GGYRCC 1 cut(s) 275
BsiEI CGRYCG 3 cut(s) 123, 524, 537
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
BslFI GGGAC 7 cut(s) 29, 340, 641, 680, 707, 736, 916
BslI CCNNNNNNNGG 6 cut(s) 129, 211, 268, 296, 336, 940
BsmFI GGGAC 7 cut(s) 29, 340, 641, 680, 707, 736, 916
BsnI GGCC 8 cut(s) 63, 81, 113, 230, 261, 638, 875, 885
BsoBI CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 78, 263, 576
Bsp143I GATC 6 cut(s) 300, 430, 534, 616, 687, 766
Bsp68I TCGCGA 1 cut(s) 533
BspANI GGCC 8 cut(s) 63, 81, 113, 230, 261, 638, 875, 885
BspCNI CTCAG 2 cut(s) 612, 661
BspFNI CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
BspPI GGATC 4 cut(s) 308, 425, 438, 774
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 275
BsrBI CCGCTC 4 cut(s) 337, 664, 714, 755
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 8 cut(s) 129, 143, 330, 733, 824, 907, 927, 967
BssMI GATC 6 cut(s) 300, 430, 534, 616, 687, 766
BssT1I CCWWGG 1 cut(s) 967
Bst2UI CCWGG 4 cut(s) 227, 331, 734, 935
Bst6I CTCTTC 2 cut(s) 66, 1020
BstDEI CTNAG 2 cut(s) 620, 648
BstDSI CCRYGG 2 cut(s) 143, 907
BstEII GGTNACC 1 cut(s) 451
BstF5I GGATG 1 cut(s) 991
BstFNI CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
BstHHI GCGC 3 cut(s) 546, 595, 803
BstKTI GATC 6 cut(s) 303, 433, 537, 619, 690, 769
BstMBI GATC 6 cut(s) 300, 430, 534, 616, 687, 766
BstMCI CGRYCG 3 cut(s) 123, 524, 537
BstMWI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 275, 402, 517
BstNI CCWGG 4 cut(s) 227, 331, 734, 935
BstPAI GACNNNNGTC 1 cut(s) 672
BstPI GGTNACC 1 cut(s) 451
BstSLI GKGCMC 2 cut(s) 263, 576
BstUI CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
BstV1I GCAGC 2 cut(s) 357, 383
BstX2I RGATCY 2 cut(s) 430, 766
BstYI RGATCY 2 cut(s) 430, 766
BsuRI GGCC 8 cut(s) 63, 81, 113, 230, 261, 638, 875, 885
BtgI CCRYGG 2 cut(s) 143, 907
BtrI CACGTC 2 cut(s) 460, 483
BtsCI GGATG 1 cut(s) 991
BtuMI TCGCGA 1 cut(s) 533
CfoI GCGC 3 cut(s) 546, 595, 803
Cfr10I RCCGGY 1 cut(s) 448
Cfr9I CCCGGG 1 cut(s) 129
CpoI CGGWCCG 1 cut(s) 892
Csp6I GTAC 6 cut(s) 106, 199, 358, 442, 718, 952
CspI CGGWCCG 1 cut(s) 892
CviQI GTAC 6 cut(s) 106, 199, 358, 442, 718, 952
DdeI CTNAG 2 cut(s) 620, 648
DpnI GATC 6 cut(s) 302, 432, 536, 618, 689, 768
DpnII GATC 6 cut(s) 300, 430, 534, 616, 687, 766
DraIII CACNNNGTG 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 66, 1020
EarI CTCTTC 2 cut(s) 66, 1020
EciI GGCGGA 1 cut(s) 765
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 967
Eco24I GRGCYC 2 cut(s) 78, 263
Eco47I GGWCC 7 cut(s) 150, 223, 327, 892, 903, 924, 931
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 1044
Eco88I CYCGRG 6 cut(s) 47, 83, 129, 158, 770, 920
Eco91I GGTNACC 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 4 cut(s) 260, 327, 903, 931
EcoO65I GGTNACC 1 cut(s) 451
EcoRII CCWGG 4 cut(s) 225, 329, 732, 933
EcoT14I CCWWGG 1 cut(s) 967
EcoT38I GRGCYC 2 cut(s) 78, 263
ErhI CCWWGG 1 cut(s) 967
FaiI YATR 2 cut(s) 561, 980
FaqI GGGAC 7 cut(s) 29, 340, 641, 680, 707, 736, 916
Fnu4HI GCNGC 5 cut(s) 270, 338, 371, 397, 504
FokI GGATG 1 cut(s) 998
FriOI GRGCYC 2 cut(s) 78, 263
Fsp4HI GCNGC 5 cut(s) 270, 338, 371, 397, 504
GlaI GCGC 3 cut(s) 545, 594, 802
GluI GCNGC 5 cut(s) 270, 338, 371, 397, 504
HaeIII GGCC 8 cut(s) 63, 81, 113, 230, 261, 638, 875, 885
HhaI GCGC 3 cut(s) 546, 595, 803
Hin6I GCGC 3 cut(s) 544, 593, 801
HinP1I GCGC 3 cut(s) 544, 593, 801
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HinfI GANTC 8 cut(s) 34, 55, 314, 349, 550, 646, 655, 916
HphI GGTGA 8 cut(s) 331, 427, 445, 697, 721, 877, 925, 949
Hpy166II GTNNAC 5 cut(s) 201, 469, 477, 613, 645
Hpy188I TCNGA 4 cut(s) 621, 651, 825, 949
Hpy188III TCNNGA 4 cut(s) 160, 532, 659, 901
Hpy8I GTNNAC 5 cut(s) 201, 469, 477, 613, 645
Hpy99I CGWCG 6 cut(s) 248, 442, 461, 484, 517, 581
HpyAV CCTTC 2 cut(s) 806, 976
HpyCH4IV ACGT 4 cut(s) 210, 440, 459, 482
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 275, 402, 517
HpyF3I CTNAG 2 cut(s) 620, 648
HpySE526I ACGT 4 cut(s) 210, 440, 459, 482
HspAI GCGC 3 cut(s) 544, 593, 801
KflI GGGWCCC 1 cut(s) 903
Kzo9I GATC 6 cut(s) 300, 430, 534, 616, 687, 766
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 5 cut(s) 334, 661, 737, 752, 963
Lsp1109I GCAGC 2 cut(s) 357, 383
MaeII ACGT 4 cut(s) 210, 440, 459, 482
MaeIII GTNAC 3 cut(s) 293, 451, 674
MalI GATC 6 cut(s) 302, 432, 536, 618, 689, 768
MbiI CCGCTC 4 cut(s) 337, 664, 714, 755
MboI GATC 6 cut(s) 300, 430, 534, 616, 687, 766
MboII GAAGA 2 cut(s) 83, 1037
MflI RGATCY 2 cut(s) 430, 766
MhlI GDGCHC 3 cut(s) 78, 263, 576
MluCI AATT 3 cut(s) 14, 248, 1001
MlyI GAGTC 4 cut(s) 308, 559, 640, 664
MmeI TCCRAC 2 cut(s) 470, 744
MslI CAYNNNNRTG 1 cut(s) 458
MvaI CCWGG 4 cut(s) 227, 331, 734, 935
MvnI CGCG 6 cut(s) 257, 533, 544, 546, 593, 803
MwoI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 275, 402, 517
NciI CCSGG 7 cut(s) 60, 97, 130, 131, 887, 896, 928
NdeII GATC 6 cut(s) 300, 430, 534, 616, 687, 766
NmeAIII GCCGAG 5 cut(s) 57, 89, 315, 664, 901
NmuCI GTSAC 2 cut(s) 451, 674
NruI TCGCGA 1 cut(s) 533
OliI CACNNNNGTG 1 cut(s) 458
PaeR7I CTCGAG 2 cut(s) 83, 158
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 6 cut(s) 116, 303, 309, 521, 530, 636
PfeI GAWTC 4 cut(s) 34, 55, 349, 916
PkrI GCNGC 5 cut(s) 271, 339, 372, 398, 505
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 4 cut(s) 308, 558, 640, 663
PpsI GAGTC 4 cut(s) 308, 558, 640, 663
PpuMI RGGWCCY 3 cut(s) 327, 903, 931
PshAI GACNNNNGTC 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 78
Psp5II RGGWCCY 3 cut(s) 327, 903, 931
Psp6I CCWGG 4 cut(s) 225, 329, 732, 933
PspEI GGTNACC 1 cut(s) 451
PspGI CCWGG 4 cut(s) 225, 329, 732, 933
PspOMI GGGCCC 1 cut(s) 259
PspPPI RGGWCCY 3 cut(s) 327, 903, 931
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 2 cut(s) 430, 766
PvuI CGATCG 1 cut(s) 537
RruI TCGCGA 1 cut(s) 533
RsaI GTAC 6 cut(s) 107, 200, 359, 443, 719, 953
RsaNI GTAC 6 cut(s) 106, 199, 358, 442, 718, 952
RseI CAYNNNNRTG 1 cut(s) 458
Rsr2I CGGWCCG 1 cut(s) 892
RsrII CGGWCCG 1 cut(s) 892
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 66
SatI GCNGC 5 cut(s) 270, 338, 371, 397, 504
Sau3AI GATC 6 cut(s) 300, 430, 534, 616, 687, 766
SchI GAGTC 4 cut(s) 308, 559, 640, 664
SduI GDGCHC 3 cut(s) 78, 263, 576
SetI ASST 8 cut(s) 78, 213, 332, 398, 411, 443, 462, 485
Sfr274I CTCGAG 2 cut(s) 83, 158
SinI GGWCC 7 cut(s) 150, 223, 327, 892, 903, 924, 931
SlaI CTCGAG 2 cut(s) 83, 158
SmaI CCCGGG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 83, 158
SmoI CTYRAG 2 cut(s) 83, 158
Sse9I AATT 3 cut(s) 14, 248, 1001
SstI GAGCTC 1 cut(s) 78
StyI CCWWGG 1 cut(s) 967
TaiI ACGT 4 cut(s) 213, 443, 462, 485
TaqI TCGA 7 cut(s) 84, 159, 246, 312, 342, 515, 795
TasI AATT 3 cut(s) 14, 248, 1001
TauI GCSGC 3 cut(s) 272, 340, 506
TfiI GAWTC 4 cut(s) 34, 55, 349, 916
TseFI GTSAC 2 cut(s) 451, 674
TseI GCWGC 2 cut(s) 370, 396
Tsp45I GTSAC 2 cut(s) 451, 674
TspDTI ATGAA 1 cut(s) 995
TspGWI ACGGA 2 cut(s) 93, 132
TspMI CCCGGG 1 cut(s) 129
VpaK11BI GGWCC 7 cut(s) 150, 223, 327, 892, 903, 924, 931
XhoI CTCGAG 2 cut(s) 83, 158
XmaI CCCGGG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.