Rh5BG558400

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003364
Physical Location & Seq
Forward (+)
88785496 .. 88786563
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG558400.1

Sequence Viewer

Length: 1068 bp
ATGCGTGAAAATCAATTCTCGGCTGAAATGGGGATTCCCAAAGTCCCCGAGTGGATTCCGGGCCTGCCGGAAGAGCTGGGCCTCGAGTGCCTGACCCGGCTTCCGTACTCGGCCCACCCCACCGCCTCCCGGGTCTGCCGTCCGTGGCGGTCCTTGCTCGAAAGCGAGGAGTTCTATCGCCACAGGAAGCAGAGCGGGTACACCCGAAACGTGGCGTGCTTGGTCCAGGCCGTTGCAGTTGCCGCCGAATTGAACGCGGGCCCCAAATCGGGTGCCGGAGGCTCGCCGAGTTACGGAATCGCCGTGTTCGACTCGGTGAGTGGGACCTGGGAGCGGCTCGACCCGATTCCCAAGTACCCGAATGGGCTGCCGATGTTCTGTCGGGTGGCGAGCTGCGAGGGCAAGCTGGTGGTGATGGGCGGGTGGGATCCGGCGACGTACAAGCCGGTCACCGACGTGTTCGTTTACGAGTTTACGACGTGTCGTTGGAGGAGGGGGGCGCCAATGCCGTCGAAGCGGTCGTTTTTCGCGATCGGGTCGCGCGGGAGTCGGGTTTATATTGCGGGCGGGCACGACGAGAGCAAGAACGCGCTGAAATCGGCGTGGGTTTACGATCTGAGGCGGGACGAGTGGGCCGAGTTGACTCAGATGAGTCGGGAGCGGGACGAGTGTCACGGGGTGGTGATCGGGGACGAGTTCTGGGTGGTGAGCGGGTACGGGACGGCGAGCCAGGGAGCGTTTGAGGGCGGAGCGGAGGCGTTGGATATCGGGTCGGGTCGGTGGAGGCGGGTCGAGGGCGCGTGGGAGGAAGGGGAGTGTCCGAGGGCGTGTGGGGGTGGAGTTGGGAGAGATGGGAGGCTGGTGAGTTGGGCCGAGTTTGGCCCGGCGGTCCGGGTCGGGACCCGTGGGGTGAATCTCGGGTCCCGGGTCCTGGTGATGGGTTCGGAGTACGATGGCGGGAGCCAAGGGGTATTCATGAAGGATGGGCAAAATGGTAAATTAGAGAGGGTTAGCGTGCCTGAAGAGTTTTCTGGGTTTATTCAGTCAGGCTGTTGCGTTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

355

Amino Acids

38.82

Weight (kDa)

5.97

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 60 1.7e-07 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 100 - 267 7.4e-15 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 102 - 243 4.9e-10 FBX42, beta-propeller domain
Kelch_1 PF01344 127 - 170 1.4e-08 Kelch motif
Beta-prop_Calicin PF13964 137 - 222 1.5e-10 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 138 - 265 5.2e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 146 - 237 9.6e-07 FKB95, Kelch-repeats domain
Kelch_1 PF01344 173 - 218 8.3e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 272, 499
AccBSI CCGCTC 5 cut(s) 195, 334, 661, 711, 752
AccII CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
AclWI GGATC 2 cut(s) 422, 435
AcuI CTGAAG 1 cut(s) 1041
AcyI GRCGYC 1 cut(s) 500
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 6 cut(s) 107, 200, 356, 440, 716, 950
AfiI CCNNNNNNNGG 8 cut(s) 129, 211, 268, 269, 293, 333, 931, 937
AflIII ACRYGT 2 cut(s) 456, 479
AgsI TTSAA 1 cut(s) 253
AjiI CACGTC 2 cut(s) 457, 480
AjnI CCWGG 4 cut(s) 225, 326, 729, 930
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 3 cut(s) 76, 393, 406
AluI AGCT 3 cut(s) 76, 393, 406
AlwI GGATC 2 cut(s) 422, 435
Ama87I CYCGRG 5 cut(s) 47, 83, 129, 917, 924
AoxI GGCC 8 cut(s) 61, 79, 111, 228, 259, 633, 870, 880
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 367, 393
AspLEI GCGC 4 cut(s) 502, 543, 592, 800
AsuC2I CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
AsuHPI GGTGA 8 cut(s) 328, 424, 442, 694, 718, 874, 922, 946
AvaI CYCGRG 5 cut(s) 47, 83, 129, 917, 924
AvaII GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
BaeGI GKGCMC 2 cut(s) 263, 573
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 2 cut(s) 272, 499
BanII GRGCYC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 354, 380
BccI CCATC 5 cut(s) 409, 845, 931, 947, 977
BceAI ACGGC 5 cut(s) 123, 215, 287, 493, 738
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 4 cut(s) 227, 328, 731, 932
BcnI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
BfoI RGCGCY 1 cut(s) 503
BisI GCNGC 4 cut(s) 243, 335, 368, 394
BlsI GCNGC 4 cut(s) 244, 336, 369, 395
Bme18I GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
BmeT110I CYCGRG 5 cut(s) 47, 83, 129, 917, 924
BmgBI CACGTC 2 cut(s) 457, 480
BoxI GACNNNNGTC 1 cut(s) 669
BpuMI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
BsaHI GRCGYC 1 cut(s) 500
BsaJI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
Bsc4I CCNNNNNNNGG 8 cut(s) 129, 211, 268, 269, 293, 333, 931, 937
Bse118I RCCGGY 1 cut(s) 445
BseBI CCWGG 4 cut(s) 227, 328, 731, 932
BseDI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
BseGI GGATG 1 cut(s) 988
BseLI CCNNNNNNNGG 8 cut(s) 129, 211, 268, 269, 293, 333, 931, 937
BseMII CTCAG 2 cut(s) 608, 659
BseRI GAGGAG 2 cut(s) 182, 505
BseSI GKGCMC 2 cut(s) 263, 573
BseXI GCAGC 2 cut(s) 354, 380
BseYI CCCAGC 1 cut(s) 76
Bsh1236I CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
Bsh1285I CGRYCG 2 cut(s) 521, 534
BshFI GGCC 8 cut(s) 63, 81, 113, 230, 261, 635, 872, 882
BshNI GGYRCC 2 cut(s) 272, 499
BsiEI CGRYCG 2 cut(s) 521, 534
BsiHKCI CYCGRG 5 cut(s) 47, 83, 129, 917, 924
BslFI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
BslI CCNNNNNNNGG 8 cut(s) 129, 211, 268, 269, 293, 333, 931, 937
BsmFI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
BsnI GGCC 8 cut(s) 63, 81, 113, 230, 261, 635, 872, 882
BsoBI CYCGRG 5 cut(s) 47, 83, 129, 917, 924
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 263, 573
Bsp143I GATC 4 cut(s) 427, 531, 613, 684
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 8 cut(s) 63, 81, 113, 230, 261, 635, 872, 882
BspCNI CTCAG 2 cut(s) 609, 658
BspFNI CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
BspHI TCATGA 1 cut(s) 975
BspPI GGATC 2 cut(s) 422, 435
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 2 cut(s) 272, 499
BsrBI CCGCTC 5 cut(s) 195, 334, 661, 711, 752
BsrFI RCCGGY 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
BssMI GATC 4 cut(s) 427, 531, 613, 684
BssNI GRCGYC 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 964
Bst2UI CCWGG 4 cut(s) 227, 328, 731, 932
Bst6I CTCTTC 2 cut(s) 66, 1017
BstACI GRCGYC 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 617, 645
BstDSI CCRYGG 2 cut(s) 143, 904
BstEII GGTNACC 1 cut(s) 448
BstF5I GGATG 1 cut(s) 988
BstFNI CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 4 cut(s) 502, 543, 592, 800
BstKTI GATC 4 cut(s) 430, 534, 616, 687
BstMBI GATC 4 cut(s) 427, 531, 613, 684
BstMCI CGRYCG 2 cut(s) 521, 534
BstMWI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 242, 399, 514
BstNI CCWGG 4 cut(s) 227, 328, 731, 932
BstPAI GACNNNNGTC 1 cut(s) 669
BstPI GGTNACC 1 cut(s) 448
BstSLI GKGCMC 2 cut(s) 263, 573
BstUI CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
BstV1I GCAGC 2 cut(s) 354, 380
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 8 cut(s) 63, 81, 113, 230, 261, 635, 872, 882
BtgI CCRYGG 2 cut(s) 143, 904
BtrI CACGTC 2 cut(s) 457, 480
BtsCI GGATG 1 cut(s) 988
BtuMI TCGCGA 1 cut(s) 530
CciI TCATGA 1 cut(s) 975
CfoI GCGC 4 cut(s) 502, 543, 592, 800
Cfr10I RCCGGY 1 cut(s) 445
Cfr9I CCCGGG 2 cut(s) 129, 924
CpoI CGGWCCG 1 cut(s) 889
Csp6I GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
CspI CGGWCCG 1 cut(s) 889
CviAII CATG 1 cut(s) 976
CviQI GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
DdeI CTNAG 2 cut(s) 617, 645
DinI GGCGCC 1 cut(s) 501
DpnI GATC 4 cut(s) 429, 533, 615, 686
DpnII GATC 4 cut(s) 427, 531, 613, 684
DraIII CACNNNGTG 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 66, 1017
EarI CTCTTC 2 cut(s) 66, 1017
EciI GGCGGA 1 cut(s) 762
Eco130I CCWWGG 1 cut(s) 964
Eco24I GRGCYC 1 cut(s) 263
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
Eco57I CTGAAG 1 cut(s) 1041
Eco88I CYCGRG 5 cut(s) 47, 83, 129, 917, 924
Eco91I GGTNACC 1 cut(s) 448
EcoO109I RGGNCCY 5 cut(s) 260, 324, 900, 921, 928
EcoO65I GGTNACC 1 cut(s) 448
EcoRII CCWGG 4 cut(s) 225, 326, 729, 930
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 263
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 964
FaeI CATG 1 cut(s) 979
FaiI YATR 2 cut(s) 558, 977
FaqI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
FatI CATG 1 cut(s) 975
Fnu4HI GCNGC 4 cut(s) 243, 335, 368, 394
FokI GGATG 1 cut(s) 995
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 4 cut(s) 243, 335, 368, 394
GlaI GCGC 4 cut(s) 501, 542, 591, 799
GluI GCNGC 4 cut(s) 243, 335, 368, 394
GsaI CCCAGC 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 8 cut(s) 63, 81, 113, 230, 261, 635, 872, 882
HhaI GCGC 4 cut(s) 502, 543, 592, 800
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 1 cut(s) 979
Hin6I GCGC 4 cut(s) 500, 541, 590, 798
HinP1I GCGC 4 cut(s) 500, 541, 590, 798
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 9 cut(s) 34, 55, 297, 311, 346, 547, 643, 652, 913
HphI GGTGA 8 cut(s) 328, 424, 442, 694, 718, 874, 922, 946
Hpy166II GTNNAC 5 cut(s) 201, 466, 474, 610, 642
Hpy188I TCNGA 4 cut(s) 618, 648, 822, 946
Hpy188III TCNNGA 4 cut(s) 529, 656, 898, 976
Hpy8I GTNNAC 5 cut(s) 201, 466, 474, 610, 642
Hpy99I CGWCG 5 cut(s) 439, 458, 481, 514, 578
HpyAV CCTTC 2 cut(s) 803, 973
HpyCH4IV ACGT 4 cut(s) 210, 437, 456, 479
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 242, 399, 514
HpyF3I CTNAG 2 cut(s) 617, 645
HpySE526I ACGT 4 cut(s) 210, 437, 456, 479
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 1 cut(s) 979
HspAI GCGC 4 cut(s) 500, 541, 590, 798
KasI GGCGCC 1 cut(s) 499
KflI GGGWCCC 2 cut(s) 900, 921
Kzo9I GATC 4 cut(s) 427, 531, 613, 684
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 5 cut(s) 331, 658, 734, 749, 960
Lsp1109I GCAGC 2 cut(s) 354, 380
MaeII ACGT 4 cut(s) 210, 437, 456, 479
MaeIII GTNAC 3 cut(s) 290, 448, 671
MalI GATC 4 cut(s) 429, 533, 615, 686
MbiI CCGCTC 5 cut(s) 195, 334, 661, 711, 752
MboI GATC 4 cut(s) 427, 531, 613, 684
MboII GAAGA 2 cut(s) 83, 1034
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 263, 573
MluCI AATT 3 cut(s) 14, 248, 998
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 4 cut(s) 305, 556, 637, 661
MmeI TCCRAC 2 cut(s) 467, 741
MslI CAYNNNNRTG 1 cut(s) 455
MvaI CCWGG 4 cut(s) 227, 328, 731, 932
MvnI CGCG 6 cut(s) 257, 530, 541, 543, 590, 800
MwoI GCNNNNNNNGC 6 cut(s) 73, 87, 154, 242, 399, 514
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
NdeII GATC 4 cut(s) 427, 531, 613, 684
NlaIII CATG 1 cut(s) 979
NmeAIII GCCGAG 4 cut(s) 89, 312, 661, 898
NmuCI GTSAC 2 cut(s) 448, 671
NruI TCGCGA 1 cut(s) 530
OliI CACNNNNGTG 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 5 cut(s) 300, 306, 518, 527, 633
PfeI GAWTC 5 cut(s) 34, 55, 297, 346, 913
PkrI GCNGC 4 cut(s) 244, 336, 369, 395
Ple19I CGATCG 1 cut(s) 534
PleI GAGTC 4 cut(s) 305, 555, 637, 660
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 4 cut(s) 305, 555, 637, 660
PpuMI RGGWCCY 4 cut(s) 324, 900, 921, 928
PshAI GACNNNNGTC 1 cut(s) 669
Psp5II RGGWCCY 4 cut(s) 324, 900, 921, 928
Psp6I CCWGG 4 cut(s) 225, 326, 729, 930
PspEI GGTNACC 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 4 cut(s) 225, 326, 729, 930
PspOMI GGGCCC 1 cut(s) 259
PspPPI RGGWCCY 4 cut(s) 324, 900, 921, 928
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 1 cut(s) 427
PvuI CGATCG 1 cut(s) 534
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 6 cut(s) 107, 200, 356, 440, 716, 950
RsaNI GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
RseI CAYNNNNRTG 1 cut(s) 455
Rsr2I CGGWCCG 1 cut(s) 889
RsrII CGGWCCG 1 cut(s) 889
SapI GCTCTTC 1 cut(s) 66
SatI GCNGC 4 cut(s) 243, 335, 368, 394
Sau3AI GATC 4 cut(s) 427, 531, 613, 684
SchI GAGTC 4 cut(s) 305, 556, 637, 661
SduI GDGCHC 2 cut(s) 263, 573
SetI ASST 8 cut(s) 78, 213, 329, 395, 408, 440, 459, 482
SfoI GGCGCC 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
SlaI CTCGAG 1 cut(s) 83
SmaI CCCGGG 2 cut(s) 131, 926
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 3 cut(s) 14, 248, 998
SspDI GGCGCC 1 cut(s) 499
StyI CCWWGG 1 cut(s) 964
TaiI ACGT 4 cut(s) 213, 440, 459, 482
TaqI TCGA 6 cut(s) 84, 159, 309, 339, 512, 792
TasI AATT 3 cut(s) 14, 248, 998
TauI GCSGC 2 cut(s) 245, 337
TfiI GAWTC 5 cut(s) 34, 55, 297, 346, 913
TseFI GTSAC 2 cut(s) 448, 671
TseI GCWGC 2 cut(s) 367, 393
Tsp45I GTSAC 2 cut(s) 448, 671
TspDTI ATGAA 2 cut(s) 964, 992
TspGWI ACGGA 3 cut(s) 93, 132, 309
TspMI CCCGGG 2 cut(s) 129, 924
VpaK11BI GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
XhoI CTCGAG 1 cut(s) 83
XmaI CCCGGG 2 cut(s) 129, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.