pycom03g00690

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003364
Physical Location & Seq
Reverse (-)
566539 .. 567732
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00690.1

Sequence Viewer

Length: 1194 bp
ATGGCCTACTACGCAATTGCACAATACACTTCTTTGATGCAAAACAACGCTTCTTTTTTTTTGGCAAACATCACTTCCTCCTTAGCTGCAAAAAGCACTTCTTGCTTAATTTGCTTTGCTGCAAAACCACACTTCTCTGCTAAAATGTGGAGTATCGAGCTTACCGATTTGATCCCGGGTCTGCCTGCAGAACTCGGACTCGAGTGTCTGACGCGGTTGCCCTACTCAGCTCATCCAGTTGCTTCCCGGGTCTGCAGTCCATGGCGGACTCTGTTGGAAAGCCAAGAATTTTACCATCACAGGAAGAAAATCGCGCGCACCCACAAAGTTGCATGCTTGGTTCAAGCTCTTCCTCGAAAGCTTGTTGCCTCAGAATCTGACGTACCTAAATCCACCGAGTCACCGAGTTTCGGAATCACCGTGTTTGACCCGGTGAGTCGTTCGTGGGATAGACTCGACCCGGTTCCTCAGTACCCAAATGGTATGCCCTTGTTTTGCCAATTGGCAAGCTGTGAAGGGAAGCTTGTGGCGATTGGCGGGTGGGACCCGGTGAGTTACAGCCCGGTCACAGACGTGTTTGTGTACGACTTCACCACTTCACGTTGGAAGAAAGCCAACGACATGCCGTCCAAGCGCTCTTTTTTTGCCATCGGATCGGACGCTGGACGGGTCTACGTGGCGGGCGGGCACGACGAGAACAAGGACGCGTTGAAGTCTGCATGGGTTTATGATGTGAGACTAGACGAGTGGACTGAGTTGACTCAGATGAGTCAGGAGCGAGACGAGTGTCAGGGGGTGATGATAGGGAGCGAGTTTTGGGTGGTTAGCGGGTATCGCACCGAGAGTCAAGGAGTCTTCGAGGCAGGTGCCGAGGTGTTGGAGTTGGGGTCGGGGCGGTGGCGGCTGGAGGACGAGGCGTGGGAGGCGGGACATTGTCCGAGGCATTGTGTTGGAGTAGGGGAAGATGGGAGATTGGTGAGTCGGGGTGAGTTGGACTCGGCGGTCCGAGTTGGAACCTGCGGTGTCATTCTTGGGGGTGGGACCCTGGTGGTGGGGTCGGAGTACCAAGGGTCAAGTCAAGGGTTCTATATGGCGGAAGGAACGAAGGGAGGGCAAAATGGCAAATTAGATAGGATTAGTGTGCCTGACGAGTTTTCTGGGTTTGTTCAATCAGGTTGCTGCGCTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

398

Amino Acids

43.49

Weight (kDa)

5.39

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 139 - 281 4.5e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 140 - 280 4e-09 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 148 - 235 1.6e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 166 - 209 1e-08 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 179 - 280 5.9e-07 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 188 - 278 1.8e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 212 - 257 7e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 854
AasI GACNNNNNNGTC 2 cut(s) 204, 1001
Acc36I ACCTGC 2 cut(s) 854, 1025
AccB1I GGYRCC 1 cut(s) 866
AccI GTMKAC 1 cut(s) 672
AccII CGCG 4 cut(s) 214, 314, 316, 707
AclWI GGATC 2 cut(s) 166, 661
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 4 cut(s) 384, 473, 584, 1064
AfeI AGCGCT 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 410, 1051
AflIII ACRYGT 2 cut(s) 573, 705
AgsI TTSAA 3 cut(s) 344, 712, 1169
AhdI GACNNNNNGTC 1 cut(s) 625
AjiI CACGTC 1 cut(s) 574
AjnI CCWGG 1 cut(s) 1044
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 7 cut(s) 86, 160, 230, 347, 361, 510, 523
AluI AGCT 7 cut(s) 86, 160, 230, 347, 361, 510, 523
Alw26I GTCTC 2 cut(s) 730, 774
AlwI GGATC 2 cut(s) 166, 661
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 3 cut(s) 175, 200, 246
Aor51HI AGCGCT 1 cut(s) 635
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 86, 119, 1179
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 4 cut(s) 316, 318, 636, 1184
AspS9I GGNCC 3 cut(s) 544, 1003, 1041
AsuC2I CCSGG 8 cut(s) 176, 177, 247, 248, 431, 461, 548, 563
AsuHPI GGTGA 8 cut(s) 393, 409, 445, 562, 583, 808, 988, 998
AvaI CYCGRG 3 cut(s) 175, 200, 246
AvaII GGWCC 3 cut(s) 544, 1003, 1041
BaeGI GKGCMC 1 cut(s) 690
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 847
BbvI GCAGC 3 cut(s) 73, 106, 1166
BccI CCATC 3 cut(s) 303, 656, 959
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 1 cut(s) 1046
BcnI CCSGG 8 cut(s) 176, 177, 247, 248, 431, 461, 548, 563
BcoDI GTCTC 2 cut(s) 730, 774
BfaI CTAG 1 cut(s) 740
BfmI CTRYAG 2 cut(s) 186, 253
BfoI RGCGCY 1 cut(s) 637
BfuAI ACCTGC 2 cut(s) 854, 1025
BisI GCNGC 4 cut(s) 87, 120, 902, 1180
BlsI GCNGC 4 cut(s) 88, 121, 903, 1181
Bme1390I CCNGG 9 cut(s) 176, 177, 247, 248, 431, 461, 548, 563, 1046
Bme18I GGWCC 3 cut(s) 544, 1003, 1041
BmeRI GACNNNNNGTC 1 cut(s) 625
BmeT110I CYCGRG 3 cut(s) 175, 200, 246
BmgBI CACGTC 1 cut(s) 574
BmgT120I GGNCC 3 cut(s) 544, 1003, 1041
BmiI GGNNCC 7 cut(s) 465, 545, 546, 868, 1015, 1042, 1043
BmrFI CCNGG 9 cut(s) 176, 177, 247, 248, 431, 461, 548, 563, 1046
BmsI GCATC 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 847
BplI GAGNNNNNCTC 2 cut(s) 980, 1012
BpmI CTGGAG 1 cut(s) 926
Bpu10I CCTNAGC 1 cut(s) 82
BpuMI CCSGG 8 cut(s) 176, 177, 247, 248, 431, 461, 548, 563
BsaAI YACGTR 1 cut(s) 676
BsaJI CCNNGG 7 cut(s) 175, 246, 260, 870, 938, 1044, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 872, 902
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 1051
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 1046
BseDI CCNNGG 7 cut(s) 175, 246, 260, 870, 938, 1044, 1066
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 2 cut(s) 410, 1051
BseMII CTCAG 6 cut(s) 240, 384, 482, 744, 776, 1176
BseNI ACTGG 1 cut(s) 236
BsePI GCGCGC 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 690
BseXI GCAGC 3 cut(s) 73, 106, 1166
Bsh1236I CGCG 4 cut(s) 214, 314, 316, 707
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 866
BsiHKCI CYCGRG 3 cut(s) 175, 200, 246
BsiSI CCGG 6 cut(s) 176, 247, 431, 461, 548, 563
BslFI GGGAC 3 cut(s) 557, 942, 1054
BslI CCNNNNNNNGG 2 cut(s) 410, 1051
BsmAI GTCTC 2 cut(s) 730, 774
BsmBI CGTCTC 1 cut(s) 774
BsmFI GGGAC 3 cut(s) 557, 942, 1054
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 3 cut(s) 175, 200, 246
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 2 cut(s) 171, 653
Bsp19I CCATGG 1 cut(s) 260
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 6 cut(s) 239, 383, 481, 745, 775, 1177
BspFNI CGCG 4 cut(s) 214, 314, 316, 707
BspLI GGNNCC 7 cut(s) 465, 545, 546, 868, 1015, 1042, 1043
BspMAI CTGCAG 2 cut(s) 190, 257
BspMI ACCTGC 2 cut(s) 854, 1025
BspPI GGATC 2 cut(s) 166, 661
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 866
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 7 cut(s) 175, 246, 260, 870, 938, 1044, 1066
BssHII GCGCGC 1 cut(s) 314
BssMI GATC 2 cut(s) 171, 653
BssT1I CCWWGG 2 cut(s) 260, 1066
Bst2UI CCWGG 1 cut(s) 1046
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 676
BstC8I GCNNGC 6 cut(s) 186, 316, 334, 508, 682, 686
BstDEI CTNAG 7 cut(s) 82, 226, 370, 468, 753, 762, 1185
BstDSI CCRYGG 1 cut(s) 260
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 4 cut(s) 214, 314, 316, 707
BstH2I RGCGCY 1 cut(s) 637
BstHHI GCGC 4 cut(s) 316, 318, 636, 1184
BstKTI GATC 2 cut(s) 174, 656
BstMAI GTCTC 2 cut(s) 730, 774
BstMBI GATC 2 cut(s) 171, 653
BstMWI GCNNNNNNNGC 7 cut(s) 11, 102, 111, 631, 834, 901, 923
BstNI CCWGG 1 cut(s) 1046
BstNSI RCATGY 2 cut(s) 336, 625
BstPAI GACNNNNGTC 1 cut(s) 786
BstSCI CCNGG 9 cut(s) 174, 175, 245, 246, 429, 459, 546, 561, 1044
BstSFI CTRYAG 2 cut(s) 186, 253
BstSLI GKGCMC 1 cut(s) 690
BstUI CGCG 4 cut(s) 214, 314, 316, 707
BstV1I GCAGC 3 cut(s) 73, 106, 1166
BstV2I GAAGAC 1 cut(s) 847
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 260
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 232
BveI ACCTGC 2 cut(s) 854, 1025
Cac8I GCNNGC 6 cut(s) 186, 316, 334, 508, 682, 686
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 4 cut(s) 316, 318, 636, 1184
Cfr13I GGNCC 3 cut(s) 544, 1003, 1041
Cfr9I CCCGGG 2 cut(s) 175, 246
CpoI CGGWCCG 1 cut(s) 1003
CseI GACGC 3 cut(s) 220, 668, 713
Csp6I GTAC 4 cut(s) 383, 472, 583, 1063
CspI CGGWCCG 1 cut(s) 1003
CviAII CATG 4 cut(s) 261, 333, 622, 720
CviQI GTAC 4 cut(s) 383, 472, 583, 1063
DdeI CTNAG 7 cut(s) 82, 226, 370, 468, 753, 762, 1185
DpnI GATC 2 cut(s) 173, 655
DpnII GATC 2 cut(s) 171, 653
DrdI GACNNNNNNGTC 2 cut(s) 204, 1001
DriI GACNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 2 cut(s) 204, 1001
Eam1104I CTCTTC 1 cut(s) 354
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 2 cut(s) 280, 1109
Eco130I CCWWGG 2 cut(s) 260, 1066
Eco47I GGWCC 3 cut(s) 544, 1003, 1041
Eco47III AGCGCT 1 cut(s) 635
Eco88I CYCGRG 3 cut(s) 175, 200, 246
EcoO109I RGGNCCY 2 cut(s) 544, 1041
EcoRII CCWGG 1 cut(s) 1044
EcoT14I CCWWGG 2 cut(s) 260, 1066
ErhI CCWWGG 2 cut(s) 260, 1066
Esp3I CGTCTC 1 cut(s) 774
FaeI CATG 4 cut(s) 264, 336, 625, 723
FaiI YATR 8 cut(s) 262, 334, 485, 623, 721, 729, 1089, 1091
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 507, 539
FaqI GGGAC 3 cut(s) 557, 942, 1054
FatI CATG 4 cut(s) 260, 332, 621, 719
FauI CCCGC 5 cut(s) 530, 673, 677, 821, 919
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 4 cut(s) 87, 120, 902, 1180
FokI GGATG 1 cut(s) 219
Fsp4HI GCNGC 4 cut(s) 87, 120, 902, 1180
FspBI CTAG 1 cut(s) 740
GlaI GCGC 4 cut(s) 315, 317, 635, 1183
GluI GCNGC 4 cut(s) 87, 120, 902, 1180
GsuI CTGGAG 1 cut(s) 926
HaeII RGCGCY 1 cut(s) 637
HaeIII GGCC 1 cut(s) 5
HapII CCGG 6 cut(s) 176, 247, 431, 461, 548, 563
HgaI GACGC 3 cut(s) 220, 668, 713
HhaI GCGC 4 cut(s) 316, 318, 636, 1184
Hin1II CATG 4 cut(s) 264, 336, 625, 723
Hin6I GCGC 4 cut(s) 314, 316, 634, 1182
HinP1I GCGC 4 cut(s) 314, 316, 634, 1182
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 2 cut(s) 359, 521
HpaII CCGG 6 cut(s) 176, 247, 431, 461, 548, 563
HphI GGTGA 8 cut(s) 393, 409, 445, 562, 583, 808, 988, 998
Hpy166II GTNNAC 4 cut(s) 583, 673, 750, 759
Hpy188III TCNNGA 1 cut(s) 773
Hpy8I GTNNAC 4 cut(s) 583, 673, 750, 759
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 3 cut(s) 509, 1091, 1099
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 4 cut(s) 381, 573, 601, 675
HpyCH4V TGCA 8 cut(s) 20, 40, 89, 122, 188, 255, 332, 719
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 102, 111, 631, 834, 901, 923
HpyF3I CTNAG 7 cut(s) 82, 226, 370, 468, 753, 762, 1185
HpySE526I ACGT 4 cut(s) 381, 573, 601, 675
Hsp92II CATG 4 cut(s) 264, 336, 625, 723
HspAI GCGC 4 cut(s) 314, 316, 634, 1182
KflI GGGWCCC 2 cut(s) 544, 1041
Kzo9I GATC 2 cut(s) 171, 653
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 775, 807
Lsp1109I GCAGC 3 cut(s) 73, 106, 1166
LweI GCATC 1 cut(s) 27
MaeI CTAG 1 cut(s) 740
MaeII ACGT 4 cut(s) 381, 573, 601, 675
MaeIII GTNAC 3 cut(s) 399, 554, 565
MalI GATC 2 cut(s) 173, 655
MboI GATC 2 cut(s) 171, 653
MboII GAAGA 5 cut(s) 316, 341, 619, 847, 974
MfeI CAATTG 2 cut(s) 15, 500
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 5 cut(s) 15, 108, 287, 500, 1124
MluI ACGCGT 1 cut(s) 705
MmeI TCCRAC 7 cut(s) 255, 584, 858, 931, 972, 991, 1038
MseI TTAA 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 572
MspI CCGG 6 cut(s) 176, 247, 431, 461, 548, 563
MspR9I CCNGG 9 cut(s) 176, 177, 247, 248, 431, 461, 548, 563, 1046
MunI CAATTG 2 cut(s) 15, 500
MvaI CCWGG 1 cut(s) 1046
MvnI CGCG 4 cut(s) 214, 314, 316, 707
MwoI GCNNNNNNNGC 7 cut(s) 11, 102, 111, 631, 834, 901, 923
NciI CCSGG 8 cut(s) 176, 177, 247, 248, 431, 461, 548, 563
NcoI CCATGG 1 cut(s) 260
NdeII GATC 2 cut(s) 171, 653
NlaIII CATG 4 cut(s) 264, 336, 625, 723
NlaIV GGNNCC 7 cut(s) 465, 545, 546, 868, 1015, 1042, 1043
NmeAIII GCCGAG 2 cut(s) 895, 977
NmuCI GTSAC 2 cut(s) 399, 565
NspI RCATGY 2 cut(s) 336, 625
OliI CACNNNNGTG 1 cut(s) 572
PaeI GCATGC 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 200
PaqCI CACCTGC 1 cut(s) 854
PauI GCGCGC 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 354
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 417
PfeI GAWTC 2 cut(s) 374, 414
PflFI GACNNNGTC 1 cut(s) 933
PkrI GCNGC 4 cut(s) 88, 121, 903, 1181
Ppu21I YACGTR 1 cut(s) 676
PpuMI RGGWCCY 2 cut(s) 544, 1041
PshAI GACNNNNGTC 1 cut(s) 786
Psp5II RGGWCCY 2 cut(s) 544, 1041
Psp6I CCWGG 1 cut(s) 1044
PspGI CCWGG 1 cut(s) 1044
PspN4I GGNNCC 7 cut(s) 465, 545, 546, 868, 1015, 1042, 1043
PspPI GGNCC 3 cut(s) 544, 1003, 1041
PspPPI RGGWCCY 2 cut(s) 544, 1041
PspXI VCTCGAGB 1 cut(s) 200
PstI CTGCAG 2 cut(s) 190, 257
PstNI CAGNNNCTG 1 cut(s) 377
PsyI GACNNNGTC 1 cut(s) 933
PteI GCGCGC 1 cut(s) 314
RsaI GTAC 4 cut(s) 384, 473, 584, 1064
RsaNI GTAC 4 cut(s) 383, 472, 583, 1063
RseI CAYNNNNRTG 1 cut(s) 572
Rsr2I CGGWCCG 1 cut(s) 1003
RsrII CGGWCCG 1 cut(s) 1003
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 4 cut(s) 87, 120, 902, 1180
Sau3AI GATC 2 cut(s) 171, 653
Sau96I GGNCC 3 cut(s) 544, 1003, 1041
ScrFI CCNGG 9 cut(s) 176, 177, 247, 248, 431, 461, 548, 563, 1046
SduI GDGCHC 1 cut(s) 690
SfaNI GCATC 1 cut(s) 27
SfcI CTRYAG 2 cut(s) 186, 253
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 3 cut(s) 544, 1003, 1041
SlaI CTCGAG 1 cut(s) 200
SmaI CCCGGG 2 cut(s) 177, 248
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SphI GCATGC 1 cut(s) 336
Sse9I AATT 5 cut(s) 15, 108, 287, 500, 1124
SspMI CTAG 1 cut(s) 740
StyD4I CCNGG 9 cut(s) 174, 175, 245, 246, 429, 459, 546, 561, 1044
StyI CCWWGG 2 cut(s) 260, 1066
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 4 cut(s) 384, 576, 604, 678
TaqI TCGA 5 cut(s) 156, 201, 355, 456, 858
TasI AATT 5 cut(s) 15, 108, 287, 500, 1124
TauI GCSGC 1 cut(s) 904
TfiI GAWTC 2 cut(s) 374, 414
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TseFI GTSAC 2 cut(s) 399, 565
TseI GCWGC 3 cut(s) 86, 119, 1179
Tsp45I GTSAC 2 cut(s) 399, 565
TspMI CCCGGG 2 cut(s) 175, 246
Tth111I GACNNNGTC 1 cut(s) 933
VpaK11BI GGWCC 3 cut(s) 544, 1003, 1041
XapI RAATTY 1 cut(s) 287
XceI RCATGY 2 cut(s) 336, 625
XhoI CTCGAG 1 cut(s) 200
XmaI CCCGGG 2 cut(s) 175, 246
XmiI GTMKAC 1 cut(s) 672
XspI CTAG 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.