MD03G1006500.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003364
Physical Location & Seq
Reverse (-)
537943 .. 539287
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1006500.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGTGGAGTATCGAGCTTACCGATTTGATTCCGGGTCTGCCTGCAGAACTCGGACTCGAGTGTCTGACTCGGTTGCCCTACTCAACTCAACCAGTTGCTTCCCGGGTCTGCCGTCCATGGCGGACTCTGTTGGAAAGCCAAGAATTTTACCATCACAGGAAGAAAATCGCACGCACCCACAAAGTTGCATGCTTGGTTCAAGCTCTTCCTCGAAAGCTTGTTGCATCAGAATCTGACGTACCCAAATCCACCGAGTCACCGAGTTTCGGAATCACCGTGTTTGACCCGGTGAGTCGTTCGTGGGATAGACTCGACCCGGTTCCTCAGTACCCAAATGGTATGCCCTTATTTTGCCAATTGGCAAGCTGTGAAGGGAAGCTTGTGGCGATTGGCGGGTGGGACCCGGTGAGTTACAACTCGGTCACTGACGTGTTTGTGTACGACTTCACCACTTCACATTGGAAGAAAGCCAACGACATGCCGTCCAAGCGCTCATTTTTTGCCATCGGATCGGACGCGGGACGGGTCTACGTGGCGGGCGGGCACGACGAGTACAAGAACGCGTTGAAGTCTGCATGGGTTTATGATGTGAGACTGGACGAGTGGACTGAGTTGACTCAGATGAGTCAGGAGCGAGACGAGTGTCAGGGGGTGGTGATTGGGAGCGAGTTTTGGGTGGTTAGCGGGTATCGCACCGAGAGTCAAGGAGTCTTCGAGGGAGGTGCCGAGGTGTTGGAGTTGGGGTCGGGGCGGTGGCGGCTGGAGGACGAGGCGTGGAAGGCGGGACATTGTCCGAGGCATTGTGTTGGAGGACTAGGGGAAGGTGGGAGATTGGTGAGTTGGGGTGAGTTGGACTCGGCGGTCCGAGTTGGAACGTGCGCTATCATGCTTGGGGGTGGGACCCTGGTGGTGGGGTCGGAGTACCAAGGGGCAAGTCAAGGGTTCTATATGGCGGAAGAAATGAAGGGAGGGCAAAATGGTAACTTAGATATGATTAGTGTGCCTGATGGGTTTTCAGGGTTTGTTCAATCAGGATGCTGCGCTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

351

Amino Acids

38.46

Weight (kDa)

5.13

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 51 1.2e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 91 - 233 1.2e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 92 - 232 2.5e-09 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 100 - 228 5.2e-12 FKB95, Kelch-repeats domain
Kelch_1 PF01344 118 - 161 6.6e-09 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 130 - 231 6.8e-07 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 141 - 229 1.3e-10 Calicin, beta-propeller domain
Kelch_1 PF01344 164 - 209 3e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 60, 860
AccB1I GGYRCC 1 cut(s) 722
AccI GTMKAC 1 cut(s) 528
AccII CGCG 2 cut(s) 518, 563
AclWI GGATC 1 cut(s) 517
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 5 cut(s) 240, 329, 440, 554, 923
AfeI AGCGCT 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 266, 910
AflIII ACRYGT 2 cut(s) 429, 561
AgsI TTSAA 3 cut(s) 200, 568, 1028
AhdI GACNNNNNGTC 1 cut(s) 481
AjiI CACGTC 1 cut(s) 430
AjnI CCWGG 1 cut(s) 903
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 5 cut(s) 16, 203, 217, 366, 379
AluI AGCT 5 cut(s) 16, 203, 217, 366, 379
Alw26I GTCTC 2 cut(s) 586, 630
AlwI GGATC 1 cut(s) 517
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 56, 102
Aor51HI AGCGCT 1 cut(s) 491
ApeKI GCWGC 1 cut(s) 1038
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 3 cut(s) 492, 881, 1043
AspS9I GGNCC 3 cut(s) 400, 862, 900
AsuC2I CCSGG 6 cut(s) 33, 103, 104, 287, 317, 404
AsuHPI GGTGA 8 cut(s) 249, 265, 301, 418, 439, 667, 847, 857
AvaI CYCGRG 2 cut(s) 56, 102
AvaII GGWCC 3 cut(s) 400, 862, 900
BaeGI GKGCMC 1 cut(s) 546
BanI GGYRCC 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 703
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 3 cut(s) 159, 512, 1001
BceAI ACGGC 2 cut(s) 96, 466
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 1 cut(s) 905
BcnI CCSGG 6 cut(s) 33, 103, 104, 287, 317, 404
BcoDI GTCTC 2 cut(s) 586, 630
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 493
BisI GCNGC 2 cut(s) 758, 1039
BlsI GCNGC 2 cut(s) 759, 1040
Bme1390I CCNGG 7 cut(s) 33, 103, 104, 287, 317, 404, 905
Bme18I GGWCC 3 cut(s) 400, 862, 900
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 2 cut(s) 56, 102
BmgBI CACGTC 1 cut(s) 430
BmgT120I GGNCC 3 cut(s) 400, 862, 900
BmiI GGNNCC 6 cut(s) 321, 401, 402, 724, 901, 902
BmrFI CCNGG 7 cut(s) 33, 103, 104, 287, 317, 404, 905
BmsI GCATC 2 cut(s) 233, 1025
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 703
BplI GAGNNNNNCTC 2 cut(s) 839, 871
BpmI CTGGAG 1 cut(s) 782
BpuMI CCSGG 6 cut(s) 33, 103, 104, 287, 317, 404
BsaAI YACGTR 1 cut(s) 532
BsaJI CCNNGG 6 cut(s) 102, 116, 726, 794, 903, 925
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 910
Bse1I ACTGG 2 cut(s) 92, 600
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 6 cut(s) 102, 116, 726, 794, 903, 925
BseGI GGATG 1 cut(s) 1040
BseLI CCNNNNNNNGG 2 cut(s) 266, 910
BseMII CTCAG 4 cut(s) 338, 600, 632, 1035
BseNI ACTGG 2 cut(s) 92, 600
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 1 cut(s) 1025
Bsh1236I CGCG 2 cut(s) 518, 563
BshNI GGYRCC 1 cut(s) 722
BsiHKCI CYCGRG 2 cut(s) 56, 102
BsiSI CCGG 5 cut(s) 32, 103, 287, 317, 404
BslFI GGGAC 4 cut(s) 413, 534, 798, 913
BslI CCNNNNNNNGG 2 cut(s) 266, 910
BsmAI GTCTC 2 cut(s) 586, 630
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 4 cut(s) 413, 534, 798, 913
BsoBI CYCGRG 2 cut(s) 56, 102
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 1 cut(s) 509
Bsp19I CCATGG 1 cut(s) 116
BspCNI CTCAG 4 cut(s) 337, 601, 631, 1036
BspFNI CGCG 2 cut(s) 518, 563
BspLI GGNNCC 6 cut(s) 321, 401, 402, 724, 901, 902
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 517
BspQI GCTCTTC 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 722
BsrI ACTGG 2 cut(s) 92, 600
BssECI CCNNGG 6 cut(s) 102, 116, 726, 794, 903, 925
BssMI GATC 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 116, 925
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 532
BstC8I GCNNGC 6 cut(s) 42, 172, 190, 364, 538, 542
BstDEI CTNAG 5 cut(s) 324, 609, 618, 985, 1044
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 1 cut(s) 1040
BstFNI CGCG 2 cut(s) 518, 563
BstH2I RGCGCY 1 cut(s) 493
BstHHI GCGC 3 cut(s) 492, 881, 1043
BstKTI GATC 1 cut(s) 512
BstMAI GTCTC 2 cut(s) 586, 630
BstMBI GATC 1 cut(s) 509
BstMWI GCNNNNNNNGC 4 cut(s) 487, 690, 757, 779
BstNI CCWGG 1 cut(s) 905
BstNSI RCATGY 2 cut(s) 192, 481
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 7 cut(s) 31, 101, 102, 285, 315, 402, 903
BstSFI CTRYAG 1 cut(s) 42
BstSLI GKGCMC 1 cut(s) 546
BstUI CGCG 2 cut(s) 518, 563
BstV1I GCAGC 1 cut(s) 1025
BstV2I GAAGAC 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 116
BtrI CACGTC 1 cut(s) 430
BtsCI GGATG 1 cut(s) 1040
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 6 cut(s) 42, 172, 190, 364, 538, 542
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 3 cut(s) 492, 881, 1043
Cfr13I GGNCC 3 cut(s) 400, 862, 900
Cfr9I CCCGGG 1 cut(s) 102
CpoI CGGWCCG 1 cut(s) 862
CseI GACGC 1 cut(s) 524
Csp6I GTAC 5 cut(s) 239, 328, 439, 553, 922
CspI CGGWCCG 1 cut(s) 862
CviAII CATG 5 cut(s) 117, 189, 478, 576, 886
CviJI RGCY 8 cut(s) 16, 138, 203, 217, 366, 379, 470, 760
CviKI_1 RGCY 8 cut(s) 16, 138, 203, 217, 366, 379, 470, 760
CviQI GTAC 5 cut(s) 239, 328, 439, 553, 922
DdeI CTNAG 5 cut(s) 324, 609, 618, 985, 1044
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
DrdI GACNNNNNNGTC 2 cut(s) 60, 860
DriI GACNNNNNGTC 1 cut(s) 481
DseDI GACNNNNNNGTC 2 cut(s) 60, 860
Eam1104I CTCTTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 2 cut(s) 136, 968
Eco130I CCWWGG 2 cut(s) 116, 925
Eco47I GGWCC 3 cut(s) 400, 862, 900
Eco47III AGCGCT 1 cut(s) 491
Eco88I CYCGRG 2 cut(s) 56, 102
EcoO109I RGGNCCY 2 cut(s) 400, 900
EcoRII CCWGG 1 cut(s) 903
EcoT14I CCWWGG 2 cut(s) 116, 925
ErhI CCWWGG 2 cut(s) 116, 925
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 5 cut(s) 120, 192, 481, 579, 889
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 4 cut(s) 413, 534, 798, 913
FatI CATG 5 cut(s) 116, 188, 477, 575, 885
FauI CCCGC 6 cut(s) 386, 511, 529, 533, 677, 775
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 758, 1039
FokI GGATG 1 cut(s) 1047
Fsp4HI GCNGC 2 cut(s) 758, 1039
FspBI CTAG 1 cut(s) 815
GlaI GCGC 3 cut(s) 491, 880, 1042
GluI GCNGC 2 cut(s) 758, 1039
GsuI CTGGAG 1 cut(s) 782
HaeII RGCGCY 1 cut(s) 493
HapII CCGG 5 cut(s) 32, 103, 287, 317, 404
HgaI GACGC 1 cut(s) 524
HhaI GCGC 3 cut(s) 492, 881, 1043
Hin1II CATG 5 cut(s) 120, 192, 481, 579, 889
Hin6I GCGC 3 cut(s) 490, 879, 1041
HinP1I GCGC 3 cut(s) 490, 879, 1041
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 2 cut(s) 215, 377
HpaII CCGG 5 cut(s) 32, 103, 287, 317, 404
HphI GGTGA 8 cut(s) 249, 265, 301, 418, 439, 667, 847, 857
Hpy166II GTNNAC 4 cut(s) 439, 529, 606, 615
Hpy188III TCNNGA 2 cut(s) 629, 1032
Hpy8I GTNNAC 4 cut(s) 439, 529, 606, 615
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 4 cut(s) 365, 772, 815, 958
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 4 cut(s) 237, 429, 531, 875
HpyCH4V TGCA 4 cut(s) 44, 188, 224, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 487, 690, 757, 779
HpyF3I CTNAG 5 cut(s) 324, 609, 618, 985, 1044
HpySE526I ACGT 4 cut(s) 237, 429, 531, 875
Hsp92II CATG 5 cut(s) 120, 192, 481, 579, 889
HspAI GCGC 3 cut(s) 490, 879, 1041
KflI GGGWCCC 2 cut(s) 400, 900
Kzo9I GATC 1 cut(s) 509
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 631, 663
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 2 cut(s) 233, 1025
MaeI CTAG 1 cut(s) 815
MaeII ACGT 4 cut(s) 237, 429, 531, 875
MaeIII GTNAC 4 cut(s) 255, 410, 421, 980
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 5 cut(s) 172, 197, 475, 703, 968
MfeI CAATTG 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 2 cut(s) 143, 356
MluI ACGCGT 1 cut(s) 561
MmeI TCCRAC 6 cut(s) 111, 714, 787, 831, 850, 897
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 5 cut(s) 32, 103, 287, 317, 404
MspR9I CCNGG 7 cut(s) 33, 103, 104, 287, 317, 404, 905
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 905
MvnI CGCG 2 cut(s) 518, 563
MwoI GCNNNNNNNGC 4 cut(s) 487, 690, 757, 779
NciI CCSGG 6 cut(s) 33, 103, 104, 287, 317, 404
NcoI CCATGG 1 cut(s) 116
NdeII GATC 1 cut(s) 509
NlaIII CATG 5 cut(s) 120, 192, 481, 579, 889
NlaIV GGNNCC 6 cut(s) 321, 401, 402, 724, 901, 902
NmeAIII GCCGAG 2 cut(s) 751, 836
NmuCI GTSAC 2 cut(s) 255, 421
NspI RCATGY 2 cut(s) 192, 481
OliI CACNNNNGTG 1 cut(s) 428
PaeI GCATGC 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 273
PfeI GAWTC 3 cut(s) 28, 230, 270
PflFI GACNNNGTC 1 cut(s) 789
PkrI GCNGC 2 cut(s) 759, 1040
Ppu21I YACGTR 1 cut(s) 532
PpuMI RGGWCCY 2 cut(s) 400, 900
PshAI GACNNNNGTC 1 cut(s) 642
Psp5II RGGWCCY 2 cut(s) 400, 900
Psp6I CCWGG 1 cut(s) 903
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 6 cut(s) 321, 401, 402, 724, 901, 902
PspPI GGNCC 3 cut(s) 400, 862, 900
PspPPI RGGWCCY 2 cut(s) 400, 900
PspXI VCTCGAGB 1 cut(s) 56
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 789
RsaI GTAC 5 cut(s) 240, 329, 440, 554, 923
RsaNI GTAC 5 cut(s) 239, 328, 439, 553, 922
RseI CAYNNNNRTG 1 cut(s) 428
Rsr2I CGGWCCG 1 cut(s) 862
RsrII CGGWCCG 1 cut(s) 862
SapI GCTCTTC 1 cut(s) 210
SatI GCNGC 2 cut(s) 758, 1039
Sau3AI GATC 1 cut(s) 509
Sau96I GGNCC 3 cut(s) 400, 862, 900
ScrFI CCNGG 7 cut(s) 33, 103, 104, 287, 317, 404, 905
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 2 cut(s) 233, 1025
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 56
SinI GGWCC 3 cut(s) 400, 862, 900
SlaI CTCGAG 1 cut(s) 56
SmaI CCCGGG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SphI GCATGC 1 cut(s) 192
Sse9I AATT 2 cut(s) 143, 356
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 7 cut(s) 31, 101, 102, 285, 315, 402, 903
StyI CCWWGG 2 cut(s) 116, 925
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 4 cut(s) 240, 432, 534, 878
TaqI TCGA 5 cut(s) 12, 57, 211, 312, 714
TaqII GACCGA 1 cut(s) 409
TasI AATT 2 cut(s) 143, 356
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 3 cut(s) 28, 230, 270
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 2 cut(s) 255, 421
TseI GCWGC 1 cut(s) 1038
Tsp45I GTSAC 2 cut(s) 255, 421
TspDTI ATGAA 1 cut(s) 977
TspMI CCCGGG 1 cut(s) 102
TspRI CASTG 1 cut(s) 430
Tth111I GACNNNGTC 1 cut(s) 789
VpaK11BI GGWCC 3 cut(s) 400, 862, 900
XapI RAATTY 1 cut(s) 143
XceI RCATGY 2 cut(s) 192, 481
XhoI CTCGAG 1 cut(s) 56
XmaI CCCGGG 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.