Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003364

Physical Location & Seq

Genomic Coordinates Reverse (-)

4838105 .. 4839277

Gene Structure 1173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1050600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGAGTGTGGTGTTTGCTTATAAATACCCAACTGAATCCCCACCCCGACCATCATCCTATCCTATCCAATCCCGGCCAAATTCTTCTACACACAAACTTTATAAGTCCAATTTGATCACCCAAAATTTGTCAATTTGCCAAATGCAAATGGCCGAGTTACTAACCGAGTTAATTCCCGGTCTGCCGGAAGAACTCGGATACGAGTGCCTGACCCGTCTGCACTATTCCACTCACCGAGTAGCCTCCCAAGTCTGCCGCCGATGGCAGCACCTTGTCCAAAGTCGGGAATTTTATAACCACCGGAAGCAATCCGGGCGGACCCACAAGGCGGCCTGCCTGGTCCAGGCTCTTCTGGTTCAGACGGGATCGGACGAGGCCAAACCGGCTAAACCGCCTGGTTATGGGGTCTCCATTTTTGATTCCGTGAGTGGGAATTGGGACCGGATCGACCCGGTTCCGAAGTACCCGCACGGGCTTCCGATGTTCTGTCAGGTGACAAGCTCGGAGGGGAAGCTGGTGGTGATGGGCGGGTGGGACCCGTCGAATTACCAGCCGATTCGGGACGTGTTCGTGTACGAGTTCACGATTCAGCGGTGGACTCGGGGGAAGGACATGCCCGAGACCCGGTCGTTCTTCGCCGCCTGTGAGCTAAACGGGCGGATATACATCGCCGGCGGCCACGACGAGAACAAGAACGCGATGAAATCGGCGATGGTTTACGATGTGAGGGAGAACGAATGGGGCGAGCTGACCGAGATGAGTCAGGGCCGAGACGAGTGCGAGGGGTTCGTATCCGGGTCGGAGTTTTGGGTCGTCAGCGGGTACGCGACGGACATGCAAGGGAGGTTCGTGGGGAGTGCGGAGGTCTATGAAACCGGGTCGGGTCGGTGGAGGCTGGAGGAGGACGTGTGGCCGGCGGGTCAGTGCCCGAGGTCGTGCGTCGGGGTTGGGAAAGACGGGAAGTTCTTCTGTTGGGGCGACTGCGATCCGAGAGTCCGGGCCGGGCCTTGCGCGCTCGGGTTGGGGAAATGGTCCTTTTTGGCGGGGTCGGCTTTCCAGGGAGCATCGCAGGAGGCTTTTTTGGTGGAAGGGCAGAAAGGTAAATTGATGAAAATGGAAACGCCGGAGGAGTTCTCTGGGTTCGTGCAGTCCGGGTGCTGCGTGGAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009966 GO:0009968 GO:0009987 GO:0010498 GO:0010646 GO:0010648 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0023057 GO:0030162 GO:0030163 GO:0031323 GO:0031461 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043455 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080036 GO:0080037 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like Kelch_1

Protein Analysis

391

Amino Acids

43.67

Weight (kDa)

6.12

Isoelectric Point (pI)

50.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	59 - 99	1.9e-06	F-box domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	108 - 298	8.9e-14	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	108 - 250	2.3e-10	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	136 - 281	2.8e-08	Attractin/LZTR1 beta-propeller
Kelch_FKB95	PF25210	151 - 304	8.9e-13	FKB95, Kelch-repeats domain
Kelch_1	PF01344	163 - 206	5.5e-07	Kelch motif
Beta-prop_Calicin	PF13964	173 - 274	9.2e-11	Calicin, beta-propeller domain
Kelch_1	PF01344	209 - 254	6.2e-11	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003364)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44130 AT3G59940
fragaria_vesca	FvH4_3g45300 FvH4_6g21491
malus_domestica	MD03G1006500.v1.1 MD11G1009500.v1.1 MD12G1051200.v1.1 MD14G1050600.v1.1
prunus_persica	Prupe.6G006200_v2.0.a1 Prupe.7G085300_v2.0.a1
pyrus_communis	pycom03g00690 pycom11g00610 pycom12g04560 pycom14g04170
rosa_chinensis	RchiOBHm_Chr3g0478681 RchiOBHm_Chr5g0082971
rosa_laevigata	RLG00000023633 RLG00000037006
rosa_multiflora	Rmu_co7966478.1_g000001 Rmu_sc0002454.1_g000004 Rmu_sc0008074.1_g000020 Rmu_sc0026666.1_g000001 Rmu_sc0026667.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403200
rosa_rugosa	Rorug03G0166900 Rorug05G0481800
rosa_samantha	Rh3AG219200 Rh3BG250900 Rh3CG247700 Rh3DG244000 Rh5AG531400 Rh5BG558400 Rh5CG581300 Rh5DG567200
rosa_wichuraiana	Rw3G019710 Rw5G049720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	21, 102, 294
AccII	CGCG	3 cut(s)	698, 827, 1013
AclWI	GGATC	3 cut(s)	373, 452, 980
AcoI	YGGCCR	4 cut(s)	74, 150, 676, 911
AcsI	RAATTY	3 cut(s)	79, 124, 287
AfaI	GTAC	3 cut(s)	464, 575, 824
AfiI	CCNNNNNNNGG	6 cut(s)	283, 328, 343, 401, 429, 624
AflIII	ACRYGT	2 cut(s)	564, 906
AjiI	CACGTC	2 cut(s)	565, 907
AjnI	CCWGG	4 cut(s)	336, 342, 394, 1056
AluBI	AGCT	4 cut(s)	501, 514, 649, 748
AluI	AGCT	4 cut(s)	501, 514, 649, 748
Alw26I	GTCTC	3 cut(s)	412, 614, 765
AlwI	GGATC	3 cut(s)	373, 452, 980
Ama87I	CYCGRG	4 cut(s)	600, 617, 928, 1016
AoxI	GGCC	9 cut(s)	74, 150, 330, 375, 676, 766, 911, 999, 1004
ApeKI	GCWGC	2 cut(s)	265, 1158
ApoI	RAATTY	3 cut(s)	79, 124, 287
Asp700I	GAANNNNTTC	1 cut(s)	965
AspLEI	GCGC	2 cut(s)	1013, 1015
AspS9I	GGNCC	8 cut(s)	318, 340, 439, 535, 766, 999, 1004, 1032
AsuHPI	GGTGA	4 cut(s)	109, 224, 505, 532
AvaI	CYCGRG	4 cut(s)	600, 617, 928, 1016
AvaII	GGWCC	5 cut(s)	318, 340, 439, 535, 1032
BaeGI	GKGCMC	1 cut(s)	929
BbvI	GCAGC	2 cut(s)	277, 1145
BccI	CCATC	4 cut(s)	58, 255, 517, 706
BcgI	CGANNNNNNTGC	4 cut(s)	759, 793, 817, 851
BciT130I	CCWGG	4 cut(s)	338, 344, 396, 1058
BciVI	GTATCC	2 cut(s)	191, 802
BclI	TGATCA	1 cut(s)	114
BcoDI	GTCTC	3 cut(s)	412, 614, 765
BfuI	GTATCC	2 cut(s)	191, 802
BglI	GCCNNNNNGGC	1 cut(s)	383
BisI	GCNGC	6 cut(s)	256, 266, 330, 639, 676, 1159
BlsI	GCNGC	6 cut(s)	257, 267, 331, 640, 677, 1160
Bme18I	GGWCC	5 cut(s)	318, 340, 439, 535, 1032
BmeT110I	CYCGRG	4 cut(s)	600, 617, 928, 1016
BmgBI	CACGTC	2 cut(s)	565, 907
BmgT120I	GGNCC	8 cut(s)	318, 340, 439, 535, 766, 999, 1004, 1032
BmiI	GGNNCC	5 cut(s)	320, 440, 456, 536, 537
BmsI	GCATC	1 cut(s)	1073
BplI	GAGNNNNNCTC	2 cut(s)	1118, 1150
BpmI	CTGGAG	1 cut(s)	917
BsaBI	GATNNNNATC	1 cut(s)	665
BsaI	GGTCTC	2 cut(s)	412, 614
BsaJI	CCNNGG	2 cut(s)	929, 1057
BsaWI	WCCGGW	2 cut(s)	300, 441
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bsc4I	CCNNNNNNNGG	6 cut(s)	283, 328, 343, 401, 429, 624
Bse118I	RCCGGY	3 cut(s)	382, 671, 913
Bse8I	GATNNNNATC	1 cut(s)	665
BseBI	CCWGG	4 cut(s)	338, 344, 396, 1058
BseDI	CCNNGG	2 cut(s)	929, 1057
BseGI	GGATG	1 cut(s)	53
BseJI	GATNNNNATC	1 cut(s)	665
BseLI	CCNNNNNNNGG	6 cut(s)	283, 328, 343, 401, 429, 624
BsePI	GCGCGC	1 cut(s)	1011
BseRI	GAGGAG	2 cut(s)	914, 1142
BseSI	GKGCMC	1 cut(s)	929
BseXI	GCAGC	2 cut(s)	277, 1145
BsgI	GTGCAG	2 cut(s)	203, 1166
Bsh1236I	CGCG	3 cut(s)	698, 827, 1013
Bsh1285I	CGRYCG	1 cut(s)	629
BshFI	GGCC	9 cut(s)	76, 152, 332, 377, 678, 768, 913, 1001, 1006
BsiEI	CGRYCG	1 cut(s)	629
BsiHKCI	CYCGRG	4 cut(s)	600, 617, 928, 1016
BslFI	GGGAC	3 cut(s)	452, 548, 575
BslI	CCNNNNNNNGG	6 cut(s)	283, 328, 343, 401, 429, 624
BsmAI	GTCTC	3 cut(s)	412, 614, 765
BsmBI	CGTCTC	1 cut(s)	765
BsmFI	GGGAC	3 cut(s)	452, 548, 575
BsnI	GGCC	9 cut(s)	76, 152, 332, 377, 678, 768, 913, 1001, 1006
Bso31I	GGTCTC	2 cut(s)	412, 614
BsoBI	CYCGRG	4 cut(s)	600, 617, 928, 1016
Bsp1286I	GDGCHC	1 cut(s)	929
Bsp143I	GATC	4 cut(s)	114, 365, 444, 985
BspANI	GGCC	9 cut(s)	76, 152, 332, 377, 678, 768, 913, 1001, 1006
BspFNI	CGCG	3 cut(s)	698, 827, 1013
BspLI	GGNNCC	5 cut(s)	320, 440, 456, 536, 537
BspPI	GGATC	3 cut(s)	373, 452, 980
BspQI	GCTCTTC	1 cut(s)	354
BspTNI	GGTCTC	2 cut(s)	412, 614
BsrFI	RCCGGY	3 cut(s)	382, 671, 913
BssAI	RCCGGY	3 cut(s)	382, 671, 913
BssECI	CCNNGG	2 cut(s)	929, 1057
BssHII	GCGCGC	1 cut(s)	1011
BssMI	GATC	4 cut(s)	114, 365, 444, 985
Bst2UI	CCWGG	4 cut(s)	338, 344, 396, 1058
Bst6I	CTCTTC	1 cut(s)	354
BstC8I	GCNNGC	5 cut(s)	334, 673, 746, 915, 1013
BstENI	CCTNNNNNAGG	1 cut(s)	341
BstF5I	GGATG	1 cut(s)	53
BstFNI	CGCG	3 cut(s)	698, 827, 1013
BstHHI	GCGC	2 cut(s)	1013, 1015
BstKTI	GATC	4 cut(s)	117, 368, 447, 988
BstMAI	GTCTC	3 cut(s)	412, 614, 765
BstMBI	GATC	4 cut(s)	114, 365, 444, 985
BstMCI	CGRYCG	1 cut(s)	629
BstMWI	GCNNNNNNNGC	5 cut(s)	313, 383, 655, 1012, 1049
BstNI	CCWGG	4 cut(s)	338, 344, 396, 1058
BstNSI	RCATGY	2 cut(s)	616, 838
BstSLI	GKGCMC	1 cut(s)	929
BstUI	CGCG	3 cut(s)	698, 827, 1013
BstV1I	GCAGC	2 cut(s)	277, 1145
BsuI	GTATCC	2 cut(s)	191, 802
BsuRI	GGCC	9 cut(s)	76, 152, 332, 377, 678, 768, 913, 1001, 1006
BtgZI	GCGATG	4 cut(s)	652, 713, 725, 1050
BtrI	CACGTC	2 cut(s)	565, 907
BtsCI	GGATG	1 cut(s)	53
BtsIMutI	CAGTG	1 cut(s)	929
Cac8I	GCNNGC	5 cut(s)	334, 673, 746, 915, 1013
CfoI	GCGC	2 cut(s)	1013, 1015
Cfr10I	RCCGGY	3 cut(s)	382, 671, 913
Cfr13I	GGNCC	8 cut(s)	318, 340, 439, 535, 766, 999, 1004, 1032
CseI	GACGC	1 cut(s)	928
Csp6I	GTAC	3 cut(s)	463, 574, 823
CviAII	CATG	2 cut(s)	613, 835
CviQI	GTAC	3 cut(s)	463, 574, 823
DpnI	GATC	4 cut(s)	116, 367, 446, 987
DpnII	GATC	4 cut(s)	114, 365, 444, 985
EaeI	YGGCCR	4 cut(s)	74, 150, 676, 911
Eam1104I	CTCTTC	1 cut(s)	354
EarI	CTCTTC	1 cut(s)	354
EciI	GGCGGA	2 cut(s)	331, 673
Eco31I	GGTCTC	2 cut(s)	412, 614
Eco47I	GGWCC	5 cut(s)	318, 340, 439, 535, 1032
Eco88I	CYCGRG	4 cut(s)	600, 617, 928, 1016
EcoNI	CCTNNNNNAGG	1 cut(s)	341
EcoO109I	RGGNCCY	1 cut(s)	535
EcoRII	CCWGG	4 cut(s)	336, 342, 394, 1056
Esp3I	CGTCTC	1 cut(s)	765
FaeI	CATG	2 cut(s)	616, 838
FaiI	YATR	8 cut(s)	21, 102, 294, 402, 614, 664, 836, 870
FaqI	GGGAC	3 cut(s)	452, 548, 575
FatI	CATG	2 cut(s)	612, 834
FauI	CCCGC	5 cut(s)	474, 521, 812, 910, 1036
FbaI	TGATCA	1 cut(s)	114
Fnu4HI	GCNGC	6 cut(s)	256, 266, 330, 639, 676, 1159
FokI	GGATG	1 cut(s)	40
Fsp4HI	GCNGC	6 cut(s)	256, 266, 330, 639, 676, 1159
GlaI	GCGC	2 cut(s)	1012, 1014
GluI	GCNGC	6 cut(s)	256, 266, 330, 639, 676, 1159
GsuI	CTGGAG	1 cut(s)	917
HaeIII	GGCC	9 cut(s)	76, 152, 332, 377, 678, 768, 913, 1001, 1006
HgaI	GACGC	1 cut(s)	928
HhaI	GCGC	2 cut(s)	1013, 1015
Hin1II	CATG	2 cut(s)	616, 838
Hin6I	GCGC	2 cut(s)	1011, 1013
HinP1I	GCGC	2 cut(s)	1011, 1013
HinfI	GANTC	7 cut(s)	35, 419, 556, 586, 598, 760, 993
HphI	GGTGA	4 cut(s)	109, 224, 505, 532
Hpy166II	GTNNAC	4 cut(s)	574, 582, 597, 718
Hpy188I	TCNGA	8 cut(s)	197, 360, 370, 459, 480, 505, 802, 990
Hpy188III	TCNNGA	3 cut(s)	284, 560, 583
Hpy8I	GTNNAC	4 cut(s)	574, 582, 597, 718
Hpy99I	CGWCG	4 cut(s)	544, 686, 832, 944
HpyAV	CCTTC	2 cut(s)	601, 1082
HpyCH4IV	ACGT	2 cut(s)	564, 906
HpyCH4V	TGCA	4 cut(s)	145, 220, 838, 1147
HpyF10VI	GCNNNNNNNGC	5 cut(s)	313, 383, 655, 1012, 1049
HpySE526I	ACGT	2 cut(s)	564, 906
Hsp92II	CATG	2 cut(s)	616, 838
HspAI	GCGC	2 cut(s)	1011, 1013
KflI	GGGWCCC	1 cut(s)	535
KroI	GCCGGC	2 cut(s)	671, 913
KroNI	GCCGGC	2 cut(s)	673, 915
Ksp22I	TGATCA	1 cut(s)	114
Kzo9I	GATC	4 cut(s)	114, 365, 444, 985
LguI	GCTCTTC	1 cut(s)	354
LmnI	GCTCC	1 cut(s)	1061
Lsp1109I	GCAGC	2 cut(s)	277, 1145
LweI	GCATC	1 cut(s)	1073
MaeII	ACGT	2 cut(s)	564, 906
MaeIII	GTNAC	2 cut(s)	156, 493
MalI	GATC	4 cut(s)	116, 367, 446, 987
MboI	GATC	4 cut(s)	114, 365, 444, 985
MboII	GAAGA	5 cut(s)	75, 200, 341, 625, 958
MhlI	GDGCHC	1 cut(s)	929
MluCI	AATT	9 cut(s)	79, 109, 124, 132, 171, 287, 433, 544, 1103
MlyI	GAGTC	3 cut(s)	592, 769, 1002
MmeI	TCCRAC	1 cut(s)	780
MreI	CGCCGGCG	1 cut(s)	671
MroNI	GCCGGC	2 cut(s)	671, 913
MroXI	GAANNNNTTC	1 cut(s)	965
MseI	TTAA	1 cut(s)	170
MspA1I	CMGCKG	2 cut(s)	592, 819
MvaI	CCWGG	4 cut(s)	338, 344, 396, 1058
MvnI	CGCG	3 cut(s)	698, 827, 1013
MwoI	GCNNNNNNNGC	5 cut(s)	313, 383, 655, 1012, 1049
NaeI	GCCGGC	2 cut(s)	673, 915
NdeII	GATC	4 cut(s)	114, 365, 444, 985
NgoMIV	GCCGGC	2 cut(s)	671, 913
NlaIII	CATG	2 cut(s)	616, 838
NlaIV	GGNNCC	5 cut(s)	320, 440, 456, 536, 537
NmeAIII	GCCGAG	2 cut(s)	178, 794
NmuCI	GTSAC	1 cut(s)	493
NspI	RCATGY	2 cut(s)	616, 838
PauI	GCGCGC	1 cut(s)	1011
PciSI	GCTCTTC	1 cut(s)	354
PcsI	WCGNNNNNNNCGW	1 cut(s)	741
PdiI	GCCGGC	2 cut(s)	673, 915
PdmI	GAANNNNTTC	1 cut(s)	965
PfeI	GAWTC	4 cut(s)	35, 419, 556, 586
PflFI	GACNNNGTC	1 cut(s)	625
PkrI	GCNGC	6 cut(s)	257, 267, 331, 640, 677, 1160
PleI	GAGTC	3 cut(s)	592, 768, 1001
PpsI	GAGTC	3 cut(s)	592, 768, 1001
PpuMI	RGGWCCY	1 cut(s)	535
PsiI	TTATAA	3 cut(s)	21, 102, 294
Psp5II	RGGWCCY	1 cut(s)	535
Psp6I	CCWGG	4 cut(s)	336, 342, 394, 1056
PspGI	CCWGG	4 cut(s)	336, 342, 394, 1056
PspN4I	GGNNCC	5 cut(s)	320, 440, 456, 536, 537
PspPI	GGNCC	8 cut(s)	318, 340, 439, 535, 766, 999, 1004, 1032
PspPPI	RGGWCCY	1 cut(s)	535
PsyI	GACNNNGTC	1 cut(s)	625
PteI	GCGCGC	1 cut(s)	1011
RsaI	GTAC	3 cut(s)	464, 575, 824
RsaNI	GTAC	3 cut(s)	463, 574, 823
SapI	GCTCTTC	1 cut(s)	354
SaqAI	TTAA	1 cut(s)	170
SatI	GCNGC	6 cut(s)	256, 266, 330, 639, 676, 1159
Sau3AI	GATC	4 cut(s)	114, 365, 444, 985
Sau96I	GGNCC	8 cut(s)	318, 340, 439, 535, 766, 999, 1004, 1032
SchI	GAGTC	3 cut(s)	592, 769, 1002
SduI	GDGCHC	1 cut(s)	929
SfaNI	GCATC	1 cut(s)	1073
SgrAI	CRCCGGYG	1 cut(s)	671
SinI	GGWCC	5 cut(s)	318, 340, 439, 535, 1032
Sse9I	AATT	9 cut(s)	79, 109, 124, 132, 171, 287, 433, 544, 1103
TaiI	ACGT	2 cut(s)	567, 909
TaqI	TCGA	2 cut(s)	447, 542
TaqII	GACCGA	1 cut(s)	767
TasI	AATT	9 cut(s)	79, 109, 124, 132, 171, 287, 433, 544, 1103
TauI	GCSGC	4 cut(s)	258, 332, 641, 678
TfiI	GAWTC	4 cut(s)	35, 419, 556, 586
Tru1I	TTAA	1 cut(s)	170
Tru9I	TTAA	1 cut(s)	170
TscAI	CASTG	1 cut(s)	929
TseFI	GTSAC	1 cut(s)	493
TseI	GCWGC	2 cut(s)	265, 1158
Tsp45I	GTSAC	1 cut(s)	493
TspDTI	ATGAA	3 cut(s)	716, 885, 1124
TspGWI	ACGGA	2 cut(s)	412, 845
TspRI	CASTG	1 cut(s)	929
Tth111I	GACNNNGTC	1 cut(s)	625
VpaK11BI	GGWCC	5 cut(s)	318, 340, 439, 535, 1032
XagI	CCTNNNNNAGG	1 cut(s)	341
XapI	RAATTY	3 cut(s)	79, 124, 287
XceI	RCATGY	2 cut(s)	616, 838
XmnI	GAANNNNTTC	1 cut(s)	965

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.