MD11G1009500.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003364
Physical Location & Seq
Reverse (-)
763748 .. 765750
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1009500.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGTGGAGTATCGAGCTGACCGATTTTATTCCGGGTTTGCCTGCAGAACTGGGACTCGAGTGCCTGACTTGGTTGCCCTACACAGCTCACCCAGTTGCCTCCCGGGTCTGCCGTCCATGGCGGACTCTCTTAGAAAGTCAAGATTTTTACTTTCACAGGAAGAAAACCGGACGTACCTACAAGGTTGCATGCTTGGTTCAAGCTCTTCCTCCAAAGCTTGTTGCATCAGAATCTGACGTACCCAAATCCACCGAGTCACCGGCCTACGGAATCACTGTGTTTGACCGGGCCAGTCGCTCGTGGGATAGACTCGACCCGGTTTCTGAGTACCCAAATGGGCTGCCCTTGTTTTGCCAATTGGCAAGTTGTGAAGGGAAGCTTGTGATCATGGGCGGTTGGGACCCGGTGAGTTACGACCCGGTCACCGACGTGTTTGTGTACGACTTCGCTACTTCCCATTGGAGGAAGGCCAACGACATGCCGTCCAAACGCTCTTTCTTCGCTATCGGATCGGACGCGGGCCAGGTGTACGTGGCGGGCGGGCATGACGAGAACAAGAACGCCTTGAAGTCAGCATGGGTTTATGATGTGAGATCGGACGAGTGGACTGAGTTGACTCCGATGAGTCATGATCGGGACGAGTGTGAAGGAGTGGTGATTGGGAGCGAGTTTTGGGTGGTTAGCGGCTACCGCACCGAGAGTCAAGGAGTGTTCGAGGGAGGTGCCGAGGTGTTGGAGTTGGGATCACGGCAGTGGCGGCTTGTGGACGAGGCGTGGGAGGCGGGGCATTGTCCAAGGCATTGTGTTGGAGTAGGGGACGATGGGAAATTGGTGAGTTGGGGTAAGTTGGACTCGGGGGTCCGAGTTGGAACGTGCGGAGTCATGCTTGGGGGTGGGACCCTGGTGGTGGGGTCGGAGTGCCAAGGGGCAAGTCAAGGGTTCTATATGGTGGAAGAAATGAAGGGACGTCAAAACGGTAAATTAGATAGTATTAGTGTGCCCGATGAGTTTTCAGGGTTTGTTCAATCAGGTTGCTGCGTTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

350

Amino Acids

38.47

Weight (kDa)

4.89

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 51 4.3e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 91 - 257 1.4e-14 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 92 - 232 6.8e-11 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 114 - 187 1.4e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 120 - 161 1.9e-08 Kelch motif
Beta-prop_Calicin PF13964 128 - 218 2.6e-11 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 129 - 256 4.4e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 164 - 209 8.3e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
AatII GACGTC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 722
AccII CGCG 1 cut(s) 518
AclWI GGATC 2 cut(s) 517, 751
AcyI GRCGYC 1 cut(s) 967
AfaI GTAC 5 cut(s) 175, 240, 329, 440, 530
AfiI CCNNNNNNNGG 3 cut(s) 266, 462, 907
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 3 cut(s) 200, 568, 1025
AhdI GACNNNNNGTC 1 cut(s) 481
AjiI CACGTC 1 cut(s) 430
AjnI CCWGG 2 cut(s) 522, 900
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AleI CACNNNNGTG 2 cut(s) 428, 751
AluBI AGCT 5 cut(s) 16, 86, 203, 217, 379
AluI AGCT 5 cut(s) 16, 86, 203, 217, 379
AlwI GGATC 2 cut(s) 517, 751
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 3 cut(s) 56, 102, 853
AoxI GGCC 4 cut(s) 261, 288, 468, 520
ApeKI GCWGC 2 cut(s) 340, 1035
AspS9I GGNCC 5 cut(s) 288, 400, 520, 859, 897
AsuC2I CCSGG 7 cut(s) 33, 103, 104, 287, 317, 404, 419
AsuHPI GGTGA 6 cut(s) 80, 249, 415, 418, 667, 844
AvaI CYCGRG 3 cut(s) 56, 102, 853
AvaII GGWCC 3 cut(s) 400, 859, 897
BaeGI GKGCMC 1 cut(s) 1002
BanI GGYRCC 1 cut(s) 722
BauI CACGAG 1 cut(s) 298
BbvI GCAGC 2 cut(s) 327, 1022
BccI CCATC 1 cut(s) 815
BceAI ACGGC 3 cut(s) 96, 466, 764
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 2 cut(s) 524, 902
BclI TGATCA 1 cut(s) 384
BcnI CCSGG 7 cut(s) 33, 103, 104, 287, 317, 404, 419
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 4 cut(s) 341, 685, 758, 1036
BlsI GCNGC 4 cut(s) 342, 686, 759, 1037
Bme1390I CCNGG 9 cut(s) 33, 103, 104, 287, 317, 404, 419, 524, 902
Bme18I GGWCC 3 cut(s) 400, 859, 897
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 3 cut(s) 56, 102, 853
BmgBI CACGTC 1 cut(s) 430
BmgT120I GGNCC 5 cut(s) 288, 400, 520, 859, 897
BmiI GGNNCC 6 cut(s) 401, 402, 724, 860, 898, 899
BmrFI CCNGG 9 cut(s) 33, 103, 104, 287, 317, 404, 419, 524, 902
BmrI ACTGGG 2 cut(s) 59, 86
BmsI GCATC 1 cut(s) 233
BmuI ACTGGG 2 cut(s) 59, 86
BpuMI CCSGG 7 cut(s) 33, 103, 104, 287, 317, 404, 419
BsaAI YACGTR 1 cut(s) 532
BsaHI GRCGYC 1 cut(s) 967
BsaJI CCNNGG 6 cut(s) 102, 116, 726, 794, 900, 922
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 462, 907
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 3 cut(s) 54, 92, 291
BseBI CCWGG 2 cut(s) 524, 902
BseDI CCNNGG 6 cut(s) 102, 116, 726, 794, 900, 922
BseLI CCNNNNNNNGG 3 cut(s) 266, 462, 907
BseMII CTCAG 2 cut(s) 315, 600
BseNI ACTGG 3 cut(s) 54, 92, 291
BseSI GKGCMC 1 cut(s) 1002
BseXI GCAGC 2 cut(s) 327, 1022
Bsh1236I CGCG 1 cut(s) 518
BshFI GGCC 4 cut(s) 263, 290, 470, 522
BshNI GGYRCC 1 cut(s) 722
BsiHKCI CYCGRG 3 cut(s) 56, 102, 853
BsiSI CCGG 8 cut(s) 32, 103, 168, 260, 286, 317, 404, 419
BslFI GGGAC 6 cut(s) 66, 413, 650, 830, 910, 978
BslI CCNNNNNNNGG 3 cut(s) 266, 462, 907
BsmFI GGGAC 6 cut(s) 66, 413, 650, 830, 910, 978
BsnI GGCC 4 cut(s) 263, 290, 470, 522
BsoBI CYCGRG 3 cut(s) 56, 102, 853
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp143I GATC 5 cut(s) 384, 509, 593, 631, 743
Bsp19I CCATGG 1 cut(s) 116
BspANI GGCC 4 cut(s) 263, 290, 470, 522
BspCNI CTCAG 2 cut(s) 316, 601
BspFNI CGCG 1 cut(s) 518
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 6 cut(s) 401, 402, 724, 860, 898, 899
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 2 cut(s) 517, 751
BspQI GCTCTTC 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 3 cut(s) 54, 92, 291
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 6 cut(s) 102, 116, 726, 794, 900, 922
BssMI GATC 5 cut(s) 384, 509, 593, 631, 743
BssNI GRCGYC 1 cut(s) 967
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 3 cut(s) 116, 794, 922
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 524, 902
Bst4CI ACNGT 2 cut(s) 277, 977
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 967
BstBAI YACGTR 1 cut(s) 532
BstC8I GCNNGC 5 cut(s) 42, 190, 520, 538, 542
BstDEI CTNAG 3 cut(s) 130, 324, 609
BstDSI CCRYGG 1 cut(s) 116
BstEII GGTNACC 1 cut(s) 421
BstFNI CGCG 1 cut(s) 518
BstKTI GATC 5 cut(s) 387, 512, 596, 634, 746
BstMBI GATC 5 cut(s) 384, 509, 593, 631, 743
BstMWI GCNNNNNNNGC 3 cut(s) 690, 757, 779
BstNI CCWGG 2 cut(s) 524, 902
BstNSI RCATGY 2 cut(s) 192, 481
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 9 cut(s) 31, 101, 102, 285, 315, 402, 417, 522, 900
BstSFI CTRYAG 1 cut(s) 42
BstSLI GKGCMC 1 cut(s) 1002
BstUI CGCG 1 cut(s) 518
BstV1I GCAGC 2 cut(s) 327, 1022
BsuRI GGCC 4 cut(s) 263, 290, 470, 522
BtgI CCRYGG 1 cut(s) 116
BtrI CACGTC 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 758
BtsIMutI CAGTG 2 cut(s) 273, 758
Cac8I GCNNGC 5 cut(s) 42, 190, 520, 538, 542
CaiI CAGNNNCTG 1 cut(s) 233
CciI TCATGA 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 5 cut(s) 288, 400, 520, 859, 897
Cfr9I CCCGGG 1 cut(s) 102
CseI GACGC 1 cut(s) 524
Csp6I GTAC 5 cut(s) 174, 239, 328, 439, 529
CviAII CATG 8 cut(s) 117, 189, 388, 478, 545, 576, 629, 883
CviQI GTAC 5 cut(s) 174, 239, 328, 439, 529
DdeI CTNAG 3 cut(s) 130, 324, 609
DpnI GATC 5 cut(s) 386, 511, 595, 633, 745
DpnII GATC 5 cut(s) 384, 509, 593, 631, 743
DrdI GACNNNNNNGTC 1 cut(s) 857
DriI GACNNNNNGTC 1 cut(s) 481
DseDI GACNNNNNNGTC 1 cut(s) 857
Eam1104I CTCTTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 116, 794, 922
Eco47I GGWCC 3 cut(s) 400, 859, 897
Eco88I CYCGRG 3 cut(s) 56, 102, 853
Eco91I GGTNACC 1 cut(s) 421
EcoO109I RGGNCCY 2 cut(s) 400, 897
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 522, 900
EcoT14I CCWWGG 3 cut(s) 116, 794, 922
ErhI CCWWGG 3 cut(s) 116, 794, 922
FaeI CATG 8 cut(s) 120, 192, 391, 481, 548, 579, 632, 886
FalI AAGNNNNNCTT 4 cut(s) 363, 395, 548, 580
FaqI GGGAC 6 cut(s) 66, 413, 650, 830, 910, 978
FatI CATG 8 cut(s) 116, 188, 387, 477, 544, 575, 628, 882
FauI CCCGC 4 cut(s) 511, 529, 533, 775
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 341, 685, 758, 1036
Fsp4HI GCNGC 4 cut(s) 341, 685, 758, 1036
GluI GCNGC 4 cut(s) 341, 685, 758, 1036
HaeIII GGCC 4 cut(s) 263, 290, 470, 522
HapII CCGG 8 cut(s) 32, 103, 168, 260, 286, 317, 404, 419
HgaI GACGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 967
Hin1II CATG 8 cut(s) 120, 192, 391, 481, 548, 579, 632, 886
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 2 cut(s) 215, 377
HpaII CCGG 8 cut(s) 32, 103, 168, 260, 286, 317, 404, 419
HphI GGTGA 6 cut(s) 80, 249, 415, 418, 667, 844
Hpy166II GTNNAC 5 cut(s) 439, 529, 606, 615, 766
Hpy188I TCNGA 9 cut(s) 229, 235, 325, 509, 514, 598, 621, 863, 916
Hpy188III TCNNGA 3 cut(s) 140, 629, 635
Hpy8I GTNNAC 5 cut(s) 439, 529, 606, 615, 766
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 4 cut(s) 365, 460, 641, 955
HpyCH4III ACNGT 2 cut(s) 277, 977
HpyCH4IV ACGT 6 cut(s) 172, 237, 429, 531, 872, 967
HpyCH4V TGCA 3 cut(s) 44, 188, 224
HpyF10VI GCNNNNNNNGC 3 cut(s) 690, 757, 779
HpyF3I CTNAG 3 cut(s) 130, 324, 609
HpySE526I ACGT 6 cut(s) 172, 237, 429, 531, 872, 967
Hsp92I GRCGYC 1 cut(s) 967
Hsp92II CATG 8 cut(s) 120, 192, 391, 481, 548, 579, 632, 886
KflI GGGWCCC 2 cut(s) 400, 897
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 5 cut(s) 384, 509, 593, 631, 743
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 663
Lsp1109I GCAGC 2 cut(s) 327, 1022
LweI GCATC 1 cut(s) 233
MaeII ACGT 6 cut(s) 172, 237, 429, 531, 872, 967
MaeIII GTNAC 3 cut(s) 255, 410, 421
MalI GATC 5 cut(s) 386, 511, 595, 633, 745
MboI GATC 5 cut(s) 384, 509, 593, 631, 743
MboII GAAGA 4 cut(s) 172, 197, 490, 965
MfeI CAATTG 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 1002
MluCI AATT 3 cut(s) 356, 827, 980
MlyI GAGTC 9 cut(s) 48, 118, 263, 303, 610, 634, 709, 845, 888
MmeI TCCRAC 5 cut(s) 714, 787, 828, 847, 894
MnlI CCTC 8 cut(s) 109, 219, 456, 709, 713, 721, 763, 772
MslI CAYNNNNRTG 2 cut(s) 428, 751
MspI CCGG 8 cut(s) 32, 103, 168, 260, 286, 317, 404, 419
MspR9I CCNGG 9 cut(s) 33, 103, 104, 287, 317, 404, 419, 524, 902
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 2 cut(s) 524, 902
MvnI CGCG 1 cut(s) 518
MwoI GCNNNNNNNGC 3 cut(s) 690, 757, 779
NciI CCSGG 7 cut(s) 33, 103, 104, 287, 317, 404, 419
NcoI CCATGG 1 cut(s) 116
NdeII GATC 5 cut(s) 384, 509, 593, 631, 743
NlaIII CATG 8 cut(s) 120, 192, 391, 481, 548, 579, 632, 886
NlaIV GGNNCC 6 cut(s) 401, 402, 724, 860, 898, 899
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 2 cut(s) 255, 421
NspI RCATGY 2 cut(s) 192, 481
OliI CACNNNNGTG 2 cut(s) 428, 751
PaeI GCATGC 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 56
PagI TCATGA 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PfeI GAWTC 2 cut(s) 230, 270
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 4 cut(s) 342, 686, 759, 1037
PleI GAGTC 9 cut(s) 48, 118, 262, 303, 610, 633, 708, 845, 887
PpsI GAGTC 9 cut(s) 48, 118, 262, 303, 610, 633, 708, 845, 887
Ppu21I YACGTR 1 cut(s) 532
PpuMI RGGWCCY 2 cut(s) 400, 897
Psp5II RGGWCCY 2 cut(s) 400, 897
Psp6I CCWGG 2 cut(s) 522, 900
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 2 cut(s) 522, 900
PspN4I GGNNCC 6 cut(s) 401, 402, 724, 860, 898, 899
PspPI GGNCC 5 cut(s) 288, 400, 520, 859, 897
PspPPI RGGWCCY 2 cut(s) 400, 897
PspXI VCTCGAGB 1 cut(s) 56
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 419
RsaI GTAC 5 cut(s) 175, 240, 329, 440, 530
RsaNI GTAC 5 cut(s) 174, 239, 328, 439, 529
RseI CAYNNNNRTG 2 cut(s) 428, 751
SapI GCTCTTC 1 cut(s) 210
SatI GCNGC 4 cut(s) 341, 685, 758, 1036
Sau3AI GATC 5 cut(s) 384, 509, 593, 631, 743
Sau96I GGNCC 5 cut(s) 288, 400, 520, 859, 897
SchI GAGTC 9 cut(s) 48, 118, 263, 303, 610, 634, 709, 845, 888
ScrFI CCNGG 9 cut(s) 33, 103, 104, 287, 317, 404, 419, 524, 902
SduI GDGCHC 1 cut(s) 1002
SfaNI GCATC 1 cut(s) 233
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 56
SinI GGWCC 3 cut(s) 400, 859, 897
SlaI CTCGAG 1 cut(s) 56
SmaI CCCGGG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 428, 751
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SphI GCATGC 1 cut(s) 192
Sse9I AATT 3 cut(s) 356, 827, 980
StyD4I CCNGG 9 cut(s) 31, 101, 102, 285, 315, 402, 417, 522, 900
StyI CCWWGG 3 cut(s) 116, 794, 922
TaaI ACNGT 2 cut(s) 277, 977
TaiI ACGT 6 cut(s) 175, 240, 432, 534, 875, 970
TaqI TCGA 4 cut(s) 12, 57, 312, 714
TaqII GACCGA 1 cut(s) 35
TasI AATT 3 cut(s) 356, 827, 980
TauI GCSGC 2 cut(s) 687, 760
TfiI GAWTC 2 cut(s) 230, 270
TscAI CASTG 2 cut(s) 280, 758
TseFI GTSAC 2 cut(s) 255, 421
TseI GCWGC 2 cut(s) 340, 1035
Tsp45I GTSAC 2 cut(s) 255, 421
TspDTI ATGAA 1 cut(s) 974
TspGWI ACGGA 1 cut(s) 282
TspMI CCCGGG 1 cut(s) 102
TspRI CASTG 2 cut(s) 280, 758
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 3 cut(s) 400, 859, 897
XceI RCATGY 2 cut(s) 192, 481
XhoI CTCGAG 1 cut(s) 56
XmaI CCCGGG 1 cut(s) 102
ZraI GACGTC 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.