RchiOBHm_Chr5g0082971

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003364
Physical Location & Seq
Forward (+)
88753317 .. 88754776
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35714

Sequence Viewer

Length: 1068 bp
ATGCATAAAAATCAATTCTCGGCTGAAATGGGGATTCCTAAAGTCCCCGAGTGGATTCCGGGCTTGCCGGAAGAGCTCGGCCTCGAGTGCCTGACCCGGCTTCCGTACTCGGCCCACCCCACCGCCTCCCGGGTCTGCCGTCCGTGGCGGTCCTTGCTCGAAAGCGAGGAGTTCTATCGCCACAGGAAGCAGAACGGGTACACCCGAAACGTGGCGTGCTTGGTCCAGGCCGTTGCGGTCGCCGTCGAATTGAACACGGGCCCCAAATCGGGTGCCGGAGGCTCGCCGAGTTACGGAATCGCCGTGTTCGACTCGGTGAGTGGGACCTGGGAGCGGCTCGACCCGATTCCCAAGTACCCGAATGGGCTGCCGATGTTTTGTCGGGTGGCGAGCTGCGAGGGCAAGCTGGTGGTGATGGGCGGGTGGGATCCGGCGACGTACAAGCCGGTCACCGACGTGTTCGTTTACGAGTTTACGACGTGTCGTTGGAGGAGGGGGGCGGCAATGCCGTCGAAGCGGTCGTTTTTCGCGATCGGGTCGCGCGGGAGTCGGGTCTACGTTGCGGGCGGGCACGACGAGAGCAAGAACGCGCTGAAATCGGCGTGGGTTTACGACCTGAGGCGGGACGAGTGGGCCGAGTTGACTCAGATGAGTCGGGAGCGGGACGAGTGTCACGGGGTGGTGATCGGGGACGAGTTCTGGGTGGTGAGCGGGTACGGGACGGCGAGCCAGGGAGCGTTTGAGGGCGGAGCGGAGGCGTTGGATCTCGGGTCGGGTCGGTGGAGGCGGGTCGAGGGCGCGTGGGAGGAAGGGGAGTGTCCGAGGGCGTGTGGGGGTGGAGTTGGGAGAGATGGGAGGCTGGTGAGTTGGGCCGAGTTTGGCCCGGCGGTCCGGGTCGGGACCCGTGGGGTGAATCTCGGGTCCCGGGTCCTGGTGATGGGTTCGGAGTACGATGGCGGGAGCCAAGGGGTATTCATGAAGGATGGGCAAAATGGTAAATTAGAGAGGGTTAGTGTGCCTGAAGAGTTTTCTGGGTTTATTCAATCAGGCTGTTGCGTTGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0019222 GO:0031323 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:2000762
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

355

Amino Acids

38.84

Weight (kDa)

6.28

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 60 1.7e-07 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 100 - 267 5.9e-15 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 102 - 243 3.7e-10 FBX42, beta-propeller domain
Kelch_1 PF01344 127 - 170 1.3e-07 Kelch motif
Beta-prop_Calicin PF13964 137 - 222 9.8e-11 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 147 - 239 7e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 173 - 218 3.6e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 272
AccBSI CCGCTC 4 cut(s) 334, 661, 711, 752
AccI GTMKAC 1 cut(s) 555
AccII CGCG 5 cut(s) 530, 541, 543, 590, 800
AclWI GGATC 3 cut(s) 422, 435, 771
AcuI CTGAAG 1 cut(s) 1041
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 6 cut(s) 107, 200, 356, 440, 716, 950
AfiI CCNNNNNNNGG 9 cut(s) 129, 211, 268, 269, 293, 333, 622, 931, 937
AflIII ACRYGT 2 cut(s) 456, 479
AgsI TTSAA 2 cut(s) 253, 1043
AjiI CACGTC 2 cut(s) 457, 480
AjnI CCWGG 4 cut(s) 225, 326, 729, 930
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 3 cut(s) 76, 393, 406
AluI AGCT 3 cut(s) 76, 393, 406
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 3 cut(s) 422, 435, 771
Ama87I CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
AoxI GGCC 7 cut(s) 79, 111, 228, 259, 633, 870, 880
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 367, 393
AspLEI GCGC 3 cut(s) 543, 592, 800
AsuC2I CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
AsuHPI GGTGA 8 cut(s) 328, 424, 442, 694, 718, 874, 922, 946
AvaI CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
AvaII GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
AxyI CCTNAGG 1 cut(s) 617
BaeGI GKGCMC 2 cut(s) 263, 573
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 2 cut(s) 78, 263
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 354, 380
BccI CCATC 5 cut(s) 409, 845, 931, 947, 977
BceAI ACGGC 6 cut(s) 123, 215, 227, 287, 493, 738
BcgI CGANNNNNNTGC 4 cut(s) 349, 383, 492, 526
BciT130I CCWGG 4 cut(s) 227, 328, 731, 932
BcnI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
BisI GCNGC 4 cut(s) 335, 368, 394, 501
BlsI GCNGC 4 cut(s) 336, 369, 395, 502
Bme18I GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
BmeT110I CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
BmgBI CACGTC 2 cut(s) 457, 480
BoxI GACNNNNGTC 1 cut(s) 669
BpuMI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
BsaJI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
Bsc4I CCNNNNNNNGG 9 cut(s) 129, 211, 268, 269, 293, 333, 622, 931, 937
Bse118I RCCGGY 1 cut(s) 445
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 4 cut(s) 227, 328, 731, 932
BseDI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
BseGI GGATG 1 cut(s) 988
BseLI CCNNNNNNNGG 9 cut(s) 129, 211, 268, 269, 293, 333, 622, 931, 937
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 608, 659
BseRI GAGGAG 2 cut(s) 182, 505
BseSI GKGCMC 2 cut(s) 263, 573
BseXI GCAGC 2 cut(s) 354, 380
Bsh1236I CGCG 5 cut(s) 530, 541, 543, 590, 800
Bsh1285I CGRYCG 3 cut(s) 240, 521, 534
BshFI GGCC 7 cut(s) 81, 113, 230, 261, 635, 872, 882
BshNI GGYRCC 1 cut(s) 272
BsiEI CGRYCG 3 cut(s) 240, 521, 534
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
BslFI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
BslI CCNNNNNNNGG 9 cut(s) 129, 211, 268, 269, 293, 333, 622, 931, 937
BsmFI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
BsnI GGCC 7 cut(s) 81, 113, 230, 261, 635, 872, 882
BsoBI CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 78, 263, 573
Bsp143I GATC 4 cut(s) 427, 531, 684, 763
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 7 cut(s) 81, 113, 230, 261, 635, 872, 882
BspCNI CTCAG 2 cut(s) 609, 658
BspFNI CGCG 5 cut(s) 530, 541, 543, 590, 800
BspHI TCATGA 1 cut(s) 975
BspPI GGATC 3 cut(s) 422, 435, 771
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 272
BsrBI CCGCTC 4 cut(s) 334, 661, 711, 752
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 8 cut(s) 129, 143, 327, 730, 821, 904, 924, 964
BssMI GATC 4 cut(s) 427, 531, 684, 763
BssT1I CCWWGG 1 cut(s) 964
Bst2UI CCWGG 4 cut(s) 227, 328, 731, 932
Bst6I CTCTTC 2 cut(s) 66, 1017
BstC8I GCNNGC 8 cut(s) 65, 217, 284, 391, 404, 565, 569, 727
BstDEI CTNAG 2 cut(s) 617, 645
BstDSI CCRYGG 2 cut(s) 143, 904
BstEII GGTNACC 1 cut(s) 448
BstF5I GGATG 1 cut(s) 988
BstFNI CGCG 5 cut(s) 530, 541, 543, 590, 800
BstHHI GCGC 3 cut(s) 543, 592, 800
BstKTI GATC 4 cut(s) 430, 534, 687, 766
BstMBI GATC 4 cut(s) 427, 531, 684, 763
BstMCI CGRYCG 3 cut(s) 240, 521, 534
BstMWI GCNNNNNNNGC 5 cut(s) 73, 87, 154, 399, 514
BstNI CCWGG 4 cut(s) 227, 328, 731, 932
BstPAI GACNNNNGTC 1 cut(s) 669
BstPI GGTNACC 1 cut(s) 448
BstSLI GKGCMC 2 cut(s) 263, 573
BstUI CGCG 5 cut(s) 530, 541, 543, 590, 800
BstV1I GCAGC 2 cut(s) 354, 380
BstX2I RGATCY 2 cut(s) 427, 763
BstYI RGATCY 2 cut(s) 427, 763
Bsu36I CCTNAGG 1 cut(s) 617
BsuRI GGCC 7 cut(s) 81, 113, 230, 261, 635, 872, 882
BtgI CCRYGG 2 cut(s) 143, 904
BtrI CACGTC 2 cut(s) 457, 480
BtsCI GGATG 1 cut(s) 988
BtuMI TCGCGA 1 cut(s) 530
Cac8I GCNNGC 8 cut(s) 65, 217, 284, 391, 404, 565, 569, 727
CciI TCATGA 1 cut(s) 975
CfoI GCGC 3 cut(s) 543, 592, 800
Cfr10I RCCGGY 1 cut(s) 445
Cfr9I CCCGGG 2 cut(s) 129, 924
CpoI CGGWCCG 1 cut(s) 889
Csp6I GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
CspI CGGWCCG 1 cut(s) 889
CviAII CATG 1 cut(s) 976
CviQI GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
DdeI CTNAG 2 cut(s) 617, 645
DpnI GATC 4 cut(s) 429, 533, 686, 765
DpnII GATC 4 cut(s) 427, 531, 684, 763
DraIII CACNNNGTG 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 66, 1017
EarI CTCTTC 2 cut(s) 66, 1017
EciI GGCGGA 1 cut(s) 762
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 964
Eco24I GRGCYC 2 cut(s) 78, 263
Eco47I GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 1041
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 6 cut(s) 47, 83, 129, 767, 917, 924
Eco91I GGTNACC 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 5 cut(s) 260, 324, 900, 921, 928
EcoO65I GGTNACC 1 cut(s) 448
EcoRII CCWGG 4 cut(s) 225, 326, 729, 930
EcoT14I CCWWGG 1 cut(s) 964
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 78, 263
ErhI CCWWGG 1 cut(s) 964
FaeI CATG 1 cut(s) 979
FaiI YATR 2 cut(s) 6, 977
FaqI GGGAC 8 cut(s) 29, 337, 638, 677, 704, 733, 907, 913
FatI CATG 1 cut(s) 975
FauI CCCGC 9 cut(s) 413, 536, 556, 560, 615, 654, 704, 780, 950
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 4 cut(s) 335, 368, 394, 501
FokI GGATG 1 cut(s) 995
FriOI GRGCYC 2 cut(s) 78, 263
Fsp4HI GCNGC 4 cut(s) 335, 368, 394, 501
GlaI GCGC 3 cut(s) 542, 591, 799
GluI GCNGC 4 cut(s) 335, 368, 394, 501
HaeIII GGCC 7 cut(s) 81, 113, 230, 261, 635, 872, 882
HhaI GCGC 3 cut(s) 543, 592, 800
Hin1II CATG 1 cut(s) 979
Hin6I GCGC 3 cut(s) 541, 590, 798
HinP1I GCGC 3 cut(s) 541, 590, 798
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 9 cut(s) 34, 55, 297, 311, 346, 547, 643, 652, 913
HphI GGTGA 8 cut(s) 328, 424, 442, 694, 718, 874, 922, 946
Hpy166II GTNNAC 6 cut(s) 201, 466, 474, 556, 610, 642
Hpy188I TCNGA 3 cut(s) 648, 822, 946
Hpy188III TCNNGA 4 cut(s) 529, 656, 898, 976
Hpy8I GTNNAC 6 cut(s) 201, 466, 474, 556, 610, 642
Hpy99I CGWCG 6 cut(s) 248, 439, 458, 481, 514, 578
HpyAV CCTTC 2 cut(s) 803, 973
HpyCH4IV ACGT 5 cut(s) 210, 437, 456, 479, 558
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 87, 154, 399, 514
HpyF3I CTNAG 2 cut(s) 617, 645
HpySE526I ACGT 5 cut(s) 210, 437, 456, 479, 558
Hsp92II CATG 1 cut(s) 979
HspAI GCGC 3 cut(s) 541, 590, 798
KflI GGGWCCC 2 cut(s) 900, 921
Kzo9I GATC 4 cut(s) 427, 531, 684, 763
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 5 cut(s) 331, 658, 734, 749, 960
Lsp1109I GCAGC 2 cut(s) 354, 380
MaeII ACGT 5 cut(s) 210, 437, 456, 479, 558
MaeIII GTNAC 3 cut(s) 290, 448, 671
MalI GATC 4 cut(s) 429, 533, 686, 765
MbiI CCGCTC 4 cut(s) 334, 661, 711, 752
MboI GATC 4 cut(s) 427, 531, 684, 763
MboII GAAGA 2 cut(s) 83, 1034
MflI RGATCY 2 cut(s) 427, 763
MhlI GDGCHC 3 cut(s) 78, 263, 573
MluCI AATT 3 cut(s) 14, 248, 998
MlyI GAGTC 4 cut(s) 305, 556, 637, 661
MmeI TCCRAC 2 cut(s) 467, 741
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 455
MvaI CCWGG 4 cut(s) 227, 328, 731, 932
MvnI CGCG 5 cut(s) 530, 541, 543, 590, 800
MwoI GCNNNNNNNGC 5 cut(s) 73, 87, 154, 399, 514
NciI CCSGG 8 cut(s) 60, 97, 130, 131, 884, 893, 925, 926
NdeII GATC 4 cut(s) 427, 531, 684, 763
NlaIII CATG 1 cut(s) 979
NmeAIII GCCGAG 5 cut(s) 57, 89, 312, 661, 898
NmuCI GTSAC 2 cut(s) 448, 671
NruI TCGCGA 1 cut(s) 530
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 5 cut(s) 300, 306, 518, 527, 633
PfeI GAWTC 5 cut(s) 34, 55, 297, 346, 913
PkrI GCNGC 4 cut(s) 336, 369, 395, 502
Ple19I CGATCG 1 cut(s) 534
PleI GAGTC 4 cut(s) 305, 555, 637, 660
PpsI GAGTC 4 cut(s) 305, 555, 637, 660
PpuMI RGGWCCY 4 cut(s) 324, 900, 921, 928
PshAI GACNNNNGTC 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 78
Psp5II RGGWCCY 4 cut(s) 324, 900, 921, 928
Psp6I CCWGG 4 cut(s) 225, 326, 729, 930
PspEI GGTNACC 1 cut(s) 448
PspGI CCWGG 4 cut(s) 225, 326, 729, 930
PspOMI GGGCCC 1 cut(s) 259
PspPPI RGGWCCY 4 cut(s) 324, 900, 921, 928
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 2 cut(s) 427, 763
PvuI CGATCG 1 cut(s) 534
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 6 cut(s) 107, 200, 356, 440, 716, 950
RsaNI GTAC 6 cut(s) 106, 199, 355, 439, 715, 949
RseI CAYNNNNRTG 1 cut(s) 455
Rsr2I CGGWCCG 1 cut(s) 889
RsrII CGGWCCG 1 cut(s) 889
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 66
SatI GCNGC 4 cut(s) 335, 368, 394, 501
Sau3AI GATC 4 cut(s) 427, 531, 684, 763
SchI GAGTC 4 cut(s) 305, 556, 637, 661
SduI GDGCHC 3 cut(s) 78, 263, 573
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
SlaI CTCGAG 1 cut(s) 83
SmaI CCCGGG 2 cut(s) 131, 926
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 3 cut(s) 14, 248, 998
SstI GAGCTC 1 cut(s) 78
StyI CCWWGG 1 cut(s) 964
TaiI ACGT 5 cut(s) 213, 440, 459, 482, 561
TaqI TCGA 7 cut(s) 84, 159, 246, 309, 339, 512, 792
TasI AATT 3 cut(s) 14, 248, 998
TauI GCSGC 2 cut(s) 337, 503
TfiI GAWTC 5 cut(s) 34, 55, 297, 346, 913
TseFI GTSAC 2 cut(s) 448, 671
TseI GCWGC 2 cut(s) 367, 393
Tsp45I GTSAC 2 cut(s) 448, 671
TspDTI ATGAA 2 cut(s) 964, 992
TspGWI ACGGA 3 cut(s) 93, 132, 309
TspMI CCCGGG 2 cut(s) 129, 924
VpaK11BI GGWCC 7 cut(s) 150, 223, 324, 889, 900, 921, 928
XhoI CTCGAG 1 cut(s) 83
XmaI CCCGGG 2 cut(s) 129, 924
XmiI GTMKAC 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.