AT2G45600

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005485
Physical Location & Seq
Forward (+)
18789489 .. 18791015
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45600.1

Sequence Viewer

Length: 990 bp
ATGGAAGCTCCTCCACCTTCAAGCGATCCATACAAGTTCCTCAACATTACACTTAACTCAGATGGATCTCTCACCAGACACCGTGATTTCCCCAAACTGCCCCCAACAGAGCAATCCAAAGACATACCATTAAACCAAACCAACAATACCTTCATCCGAATCTTCAAGCCACGGAATATTCCGCCGGAATCCAAACTTCCGATCCTCGTTTACTTTCACGGCGGTGGATTTATCCTCTACAGCGCCGCTTCAGCTCCTTTCCATGAATCTTGCACCAAAATGGCTGATCGTCTTCAAACTATAATCCTCTCCGTCGAATACCGTCTAGCTCCCGAACACCGTCTCCCGGCGGCGTACGAAGACGCCGTCGAAGCTATCTTATGGCTCCGTGATCAAGCTCGTGGGCCAATCAACGGTGGTGATTGCGACACGTGGTTAAAAGACGGTGTCGATTTCTCGAAATGCTACGTCATGGGTTCAAGCTCTGGAGGAAATATTGTCTACAACGTGGCGTTGCGTGTAGTTGACACAGATCTTTCTCCTGTTAAGATCCAAGGGCTGATAATGAACCAAGCTTTCTTCGGTGGCGTTGAGCCGTCGGATTCTGAGTCACGGCTTAAAGACGATAAGATCTGTCCGTTACCAGCTACTCACTTGTTGTGGTCACTTTGTTTACCGGACGGTGTAGATCGTGACCACGTGTACAGTAATCCAATTAAGAGCAGTGGGCCTCAGGAAAAAGATAAGATGGGACGTTTTCCGTCGACTCTTATCAACGGTTACGGTGGGGATCCTTTAGTGGATCGTCAGAGACACGTGGCGGAGATGTTAAAGGGACGTGGAGTTCACGTGGAGACGAGGTTTGATAAAGATGGGTTTCATGCTTGTGAGTTGTTTGATGGGAACAAAGCTAAGGCCCTGTACGAAACCGTTGAGGCCTTTATGAAGAGTTGTTCATCAACTGGCCCATCGTCCAACTCCAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

329

Amino Acids

36.42

Weight (kDa)

6.0

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 52 - 110 3.4e-06 Carboxylesterase family
BD-FAE PF20434 63 - 172 1.6e-10 BD-FAE
Abhydrolase_3 PF07859 69 - 296 4.3e-56 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 501, 764
AciI CCGC 5 cut(s) 182, 222, 246, 350, 821
AclWI GGATC 7 cut(s) 20, 73, 196, 544, 785, 798, 810
AcuI CTGAAG 1 cut(s) 234
AcvI CACGTG 4 cut(s) 432, 700, 817, 850
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 3 cut(s) 356, 704, 923
AfiI CCNNNNNNNGG 2 cut(s) 346, 413
AflIII ACRYGT 4 cut(s) 429, 699, 814, 984
AgsI TTSAA 4 cut(s) 21, 166, 296, 480
AjiI CACGTC 1 cut(s) 839
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AleI CACNNNNGTG 1 cut(s) 222
AloI GAACNNNNNNTCC 4 cut(s) 327, 359, 828, 860
AluBI AGCT 9 cut(s) 8, 254, 329, 374, 398, 483, 575, 647, 911
AluI AGCT 9 cut(s) 8, 254, 329, 374, 398, 483, 575, 647, 911
Alw26I GTCTC 3 cut(s) 347, 805, 848
AlwI GGATC 7 cut(s) 20, 73, 196, 544, 785, 798, 810
AoxI GGCC 5 cut(s) 404, 728, 915, 936, 964
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 4 cut(s) 404, 728, 916, 965
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 2 cut(s) 64, 431
AxyI CCTNAGG 1 cut(s) 732
BamHI GGATCC 1 cut(s) 790
BauI CACGAG 1 cut(s) 399
BbrPI CACGTG 4 cut(s) 432, 700, 817, 850
BbsI GAAGAC 2 cut(s) 284, 366
BccI CCATC 5 cut(s) 56, 742, 866, 893, 976
BceAI ACGGC 4 cut(s) 235, 350, 580, 629
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 347, 805, 848
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 246
BglII AGATCT 2 cut(s) 532, 630
BisI GCNGC 2 cut(s) 246, 351
BlsI GCNGC 2 cut(s) 247, 352
Bme1390I CCNGG 1 cut(s) 347
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 4 cut(s) 404, 728, 916, 965
BmiI GGNNCC 2 cut(s) 386, 792
BmrFI CCNGG 1 cut(s) 347
BpiI GAAGAC 2 cut(s) 284, 366
BpmI CTGGAG 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 912
BpuMI CCSGG 1 cut(s) 347
BsaAI YACGTR 4 cut(s) 432, 700, 817, 850
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 170, 553
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 6 cut(s) 327, 357, 834, 845, 864, 875
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 413
Bse1I ACTGG 1 cut(s) 967
Bse21I CCTNAGG 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 170, 553
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 346, 413
BseMII CTCAG 3 cut(s) 72, 597, 746
BseNI ACTGG 1 cut(s) 967
BshFI GGCC 5 cut(s) 406, 730, 917, 938, 966
BsiSI CCGG 3 cut(s) 185, 347, 677
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 2 cut(s) 765, 849
BslI CCNNNNNNNGG 2 cut(s) 346, 413
BsmAI GTCTC 3 cut(s) 347, 805, 848
BsmBI CGTCTC 2 cut(s) 347, 848
BsmFI GGGAC 2 cut(s) 765, 849
BsnI GGCC 5 cut(s) 406, 730, 917, 938, 966
Bsp1407I TGTACA 1 cut(s) 702
BspACI CCGC 5 cut(s) 182, 222, 246, 350, 821
BspANI GGCC 5 cut(s) 406, 730, 917, 938, 966
BspCNI CTCAG 3 cut(s) 71, 598, 745
BspLI GGNNCC 2 cut(s) 386, 792
BspPI GGATC 7 cut(s) 20, 73, 196, 544, 785, 798, 810
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 1 cut(s) 967
BssECI CCNNGG 2 cut(s) 170, 553
BssNI GRCGYC 1 cut(s) 363
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 941
BstACI GRCGYC 1 cut(s) 363
BstAUI TGTACA 1 cut(s) 702
BstBAI YACGTR 4 cut(s) 432, 700, 817, 850
BstDEI CTNAG 4 cut(s) 58, 606, 732, 912
BstDSI CCRYGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstMAI GTCTC 3 cut(s) 347, 805, 848
BstMWI GCNNNNNNNGC 2 cut(s) 251, 371
BstNSI RCATGY 1 cut(s) 988
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 1 cut(s) 238
BstV2I GAAGAC 2 cut(s) 284, 366
BstX2I RGATCY 5 cut(s) 65, 532, 549, 630, 790
BstYI RGATCY 5 cut(s) 65, 532, 549, 630, 790
Bsu36I CCTNAGG 1 cut(s) 732
BsuRI GGCC 5 cut(s) 406, 730, 917, 938, 966
BtgI CCRYGG 1 cut(s) 170
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 1 cut(s) 153
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 1 cut(s) 730
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 4 cut(s) 404, 728, 916, 965
CseI GACGC 1 cut(s) 371
Csp6I GTAC 3 cut(s) 355, 703, 922
CviAII CATG 4 cut(s) 263, 472, 881, 985
CviQI GTAC 3 cut(s) 355, 703, 922
DdeI CTNAG 4 cut(s) 58, 606, 732, 912
Eam1104I CTCTTC 1 cut(s) 941
EarI CTCTTC 1 cut(s) 941
EciI GGCGGA 2 cut(s) 171, 836
Eco130I CCWWGG 1 cut(s) 553
Eco147I AGGCCT 1 cut(s) 938
Eco57I CTGAAG 1 cut(s) 234
Eco72I CACGTG 4 cut(s) 432, 700, 817, 850
Eco81I CCTNAGG 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 916
EcoT14I CCWWGG 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 2 cut(s) 347, 848
FaeI CATG 4 cut(s) 266, 475, 884, 988
FaiI YATR 9 cut(s) 31, 125, 264, 302, 382, 473, 882, 944, 986
FaqI GGGAC 2 cut(s) 765, 849
FatI CATG 4 cut(s) 262, 471, 880, 984
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 2 cut(s) 501, 764
Fnu4HI GCNGC 2 cut(s) 246, 351
FokI GGATG 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 246, 351
FspBI CTAG 1 cut(s) 326
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 246, 351
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 5 cut(s) 406, 730, 917, 938, 966
HapII CCGG 3 cut(s) 185, 347, 677
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 4 cut(s) 266, 475, 884, 988
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 2 cut(s) 526, 765
HindII GTYRAC 2 cut(s) 526, 765
HindIII AAGCTT 1 cut(s) 573
HinfI GANTC 6 cut(s) 159, 188, 266, 602, 608, 766
HpaII CCGG 3 cut(s) 185, 347, 677
HphI GGTGA 2 cut(s) 64, 431
Hpy166II GTNNAC 7 cut(s) 211, 502, 526, 674, 703, 765, 847
Hpy188I TCNGA 6 cut(s) 61, 158, 201, 601, 607, 810
Hpy188III TCNNGA 5 cut(s) 332, 457, 486, 692, 734
Hpy8I GTNNAC 7 cut(s) 211, 502, 526, 674, 703, 765, 847
Hpy99I CGWCG 4 cut(s) 317, 371, 601, 766
HpyAV CCTTC 2 cut(s) 27, 160
HpyCH4IV ACGT 8 cut(s) 431, 468, 507, 699, 754, 816, 838, 849
HpyCH4V TGCA 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 371
HpyF3I CTNAG 4 cut(s) 58, 606, 732, 912
HpySE526I ACGT 8 cut(s) 431, 468, 507, 699, 754, 816, 838, 849
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 4 cut(s) 266, 475, 884, 988
HspAI GCGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 391
LmnI GCTCC 4 cut(s) 13, 259, 334, 390
MaeI CTAG 1 cut(s) 326
MaeII ACGT 8 cut(s) 431, 468, 507, 699, 754, 816, 838, 849
MaeIII GTNAC 5 cut(s) 609, 639, 663, 692, 779
MboII GAAGA 5 cut(s) 154, 284, 371, 571, 958
MflI RGATCY 5 cut(s) 65, 532, 549, 630, 790
MluCI AATT 1 cut(s) 714
MlyI GAGTC 2 cut(s) 617, 760
MmeI TCCRAC 1 cut(s) 579
MnlI CCTC 9 cut(s) 21, 50, 215, 245, 317, 482, 741, 852, 928
MseI TTAA 7 cut(s) 54, 131, 437, 546, 618, 717, 830
MslI CAYNNNNRTG 3 cut(s) 222, 278, 885
MspI CCGG 3 cut(s) 185, 347, 677
MspR9I CCNGG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 251, 371
NciI CCSGG 1 cut(s) 347
NlaIII CATG 4 cut(s) 266, 475, 884, 988
NlaIV GGNNCC 2 cut(s) 386, 792
NmuCI GTSAC 3 cut(s) 609, 663, 692
NspI RCATGY 1 cut(s) 988
OliI CACNNNNGTG 1 cut(s) 222
PceI AGGCCT 1 cut(s) 938
PciI ACATGT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 4 cut(s) 159, 188, 266, 602
Pfl23II CGTACG 1 cut(s) 354
PflFI GACNNNGTC 2 cut(s) 365, 446
PkrI GCNGC 2 cut(s) 247, 352
PleI GAGTC 2 cut(s) 616, 760
PmaCI CACGTG 4 cut(s) 432, 700, 817, 850
PmlI CACGTG 4 cut(s) 432, 700, 817, 850
PpsI GAGTC 2 cut(s) 616, 760
Ppu21I YACGTR 4 cut(s) 432, 700, 817, 850
PscI ACATGT 1 cut(s) 984
PspCI CACGTG 4 cut(s) 432, 700, 817, 850
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 386, 792
PspPI GGNCC 4 cut(s) 404, 728, 916, 965
PsuI RGATCY 5 cut(s) 65, 532, 549, 630, 790
PsyI GACNNNGTC 2 cut(s) 365, 446
RsaI GTAC 3 cut(s) 356, 704, 923
RsaNI GTAC 3 cut(s) 355, 703, 922
RseI CAYNNNNRTG 3 cut(s) 222, 278, 885
SalI GTCGAC 1 cut(s) 763
SaqAI TTAA 7 cut(s) 54, 131, 437, 546, 618, 717, 830
SatI GCNGC 2 cut(s) 246, 351
Sau96I GGNCC 4 cut(s) 404, 728, 916, 965
SchI GAGTC 2 cut(s) 617, 760
ScrFI CCNGG 1 cut(s) 347
SfcI CTRYAG 1 cut(s) 238
SmiMI CAYNNNNRTG 3 cut(s) 222, 278, 885
Sse9I AATT 1 cut(s) 714
SseBI AGGCCT 1 cut(s) 938
SsiI CCGC 5 cut(s) 182, 222, 246, 350, 821
SspI AATATT 2 cut(s) 178, 496
SspMI CTAG 1 cut(s) 326
StuI AGGCCT 1 cut(s) 938
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 1 cut(s) 553
TaiI ACGT 8 cut(s) 434, 471, 510, 702, 757, 819, 841, 852
TaqI TCGA 5 cut(s) 315, 369, 450, 458, 764
TasI AATT 1 cut(s) 714
TatI WGTACW 1 cut(s) 702
TauI GCSGC 2 cut(s) 248, 353
TfiI GAWTC 4 cut(s) 159, 188, 266, 602
Tru1I TTAA 7 cut(s) 54, 131, 437, 546, 618, 717, 830
Tru9I TTAA 7 cut(s) 54, 131, 437, 546, 618, 717, 830
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 3 cut(s) 609, 663, 692
Tsp45I GTSAC 3 cut(s) 609, 663, 692
TspDTI ATGAA 6 cut(s) 142, 279, 581, 869, 945, 959
TspGWI ACGGA 5 cut(s) 187, 301, 377, 627, 750
TspRI CASTG 1 cut(s) 730
Tth111I GACNNNGTC 2 cut(s) 365, 446
XceI RCATGY 1 cut(s) 988
XmiI GTMKAC 2 cut(s) 501, 764
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.