Rw1G020240

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005485
Physical Location & Seq
Forward (+)
43829770 .. 43830794
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020240.1

Sequence Viewer

Length: 849 bp
ATGGCAGATCAACAATCCTCAATCGACCCCTACAAGTTCCTCAAAATCGTTCCCAACCCAGATGGCTCACTCACCCGGCTCCCCCAATTCCCAATCGCACCCACAACCGACGCTGCCTCTCAGCCTTTCCACAACTCCTGCTCTCGCCTCGCCCTCTCTATCCCAGCCCTAGTCCTCTCCGTCGAGTACCGTCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGCCGTTTCGGCCATCACCTGGCTCAAAAACCAGGCCTCCTCTATCGACGGAGATTCGTGGCTGAAAAACTCCGTAGATTTCTCCAAGTGTATCCTGATGGGAACTAGCGCCGGGGCTAATATCGTTTACCACGCGGCTCTACGCGCTTCCGATGTGGACCTCTCCCCGATCAAGATCCGAGGGCTGATATTGGACCAGGCGTATTTCGGTGGGGTCAACAGGACGGAATCCGAGATCAGGTTCGTCAACGACCAGGTGCTGCCGTTAGTCGCTAACGACTTGATGTGGGCCCTGAGTCTGCCCGAGGGGGTTGATCGTGACCACGAGTATTGTAATCCGTTTATGAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTTAGCGGGTACGGCGGCGACCCGCTGGTGGATAGGCAGAAGGAGATCGCGGCACTGTTGGAGTCACGCGGGGTGCACGTGATGGCCCGGTTTAAGGAGGGTGGTTTTCATGCTGTGGAGCTATTTGAACCGAGTGAGGCCGAGGCTTTGTATGAGATTGTTAAGGAATTCATCGGCTCTTGTTGCGCACGCGGTAGCGATGATGTTGCCGTGGCAAAATCAGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

282

Amino Acids

30.73

Weight (kDa)

4.99

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 40 - 245 1.9e-45 alpha/beta hydrolase fold
BD-FAE PF20434 58 - 128 9.3e-08 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 249
AccII CGCG 5 cut(s) 365, 375, 668, 687, 810
AclWI GGATC 2 cut(s) 400, 602
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 785
AcvI CACGTG 1 cut(s) 697
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 2 cut(s) 188, 629
AfiI CCNNNNNNNGG 4 cut(s) 249, 468, 646, 712
AgsI TTSAA 1 cut(s) 746
AjnI CCWGG 4 cut(s) 248, 261, 426, 483
AluBI AGCT 1 cut(s) 739
AluI AGCT 1 cut(s) 739
Alw21I GWGCWC 2 cut(s) 207, 696
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 2 cut(s) 400, 602
AlwNI CAGNNNCTG 1 cut(s) 490
Ama87I CYCGRG 2 cut(s) 200, 533
AoxI GGCC 5 cut(s) 240, 264, 519, 702, 756
ApaI GGGCCC 1 cut(s) 523
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 2 cut(s) 113, 490
ApoI RAATTY 1 cut(s) 785
AspLEI GCGC 3 cut(s) 341, 377, 806
AspS9I GGNCC 5 cut(s) 388, 424, 519, 520, 703
AsuC2I CCSGG 3 cut(s) 76, 343, 706
AsuHPI GGTGA 3 cut(s) 64, 238, 596
AvaI CYCGRG 2 cut(s) 200, 533
AvaII GGWCC 2 cut(s) 388, 424
BaeGI GKGCMC 2 cut(s) 523, 696
BanII GRGCYC 1 cut(s) 523
BauI CACGAG 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 697
Bbv12I GWGCWC 2 cut(s) 207, 696
BbvI GCAGC 2 cut(s) 100, 477
BccI CCATC 5 cut(s) 56, 251, 322, 581, 694
BceAI ACGGC 6 cut(s) 218, 478, 592, 595, 646, 812
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 4 cut(s) 250, 263, 428, 485
BciVI GTATCC 1 cut(s) 332
BcnI CCSGG 3 cut(s) 76, 343, 706
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 2 cut(s) 170, 336
BfoI RGCGCY 1 cut(s) 342
BfuI GTATCC 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 6 cut(s) 114, 219, 366, 491, 634, 669
BlsI GCNGC 6 cut(s) 115, 220, 367, 492, 635, 670
Bme1390I CCNGG 7 cut(s) 76, 250, 263, 343, 428, 485, 706
Bme18I GGWCC 2 cut(s) 388, 424
BmeT110I CYCGRG 2 cut(s) 200, 533
BmgT120I GGNCC 5 cut(s) 388, 424, 519, 520, 703
BmiI GGNNCC 2 cut(s) 80, 521
BmrFI CCNGG 7 cut(s) 76, 250, 263, 343, 428, 485, 706
BpuMI CCSGG 3 cut(s) 76, 343, 706
BsaAI YACGTR 1 cut(s) 697
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 6 cut(s) 342, 409, 534, 608, 759, 828
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 468, 646, 712
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 4 cut(s) 250, 263, 428, 485
BseDI CCNNGG 6 cut(s) 342, 409, 534, 608, 759, 828
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 4 cut(s) 249, 468, 646, 712
BseMII CTCAG 2 cut(s) 134, 515
BseRI GAGGAG 1 cut(s) 259
BseSI GKGCMC 2 cut(s) 523, 696
BseXI GCAGC 2 cut(s) 100, 477
BseYI CCCAGC 1 cut(s) 163
Bsh1236I CGCG 5 cut(s) 365, 375, 668, 687, 810
BshFI GGCC 5 cut(s) 242, 266, 521, 704, 758
BsiHKAI GWGCWC 2 cut(s) 207, 696
BsiHKCI CYCGRG 2 cut(s) 200, 533
BsiSI CCGG 3 cut(s) 76, 342, 706
BslI CCNNNNNNNGG 4 cut(s) 249, 468, 646, 712
BsmAI GTCTC 1 cut(s) 197
BsmBI CGTCTC 1 cut(s) 197
BsnI GGCC 5 cut(s) 242, 266, 521, 704, 758
BsoBI CYCGRG 2 cut(s) 200, 533
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 3 cut(s) 207, 523, 696
Bsp143I GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
BspANI GGCC 5 cut(s) 242, 266, 521, 704, 758
BspCNI CTCAG 2 cut(s) 133, 516
BspFNI CGCG 5 cut(s) 365, 375, 668, 687, 810
BspLI GGNNCC 2 cut(s) 80, 521
BspPI GGATC 2 cut(s) 400, 602
BssECI CCNNGG 6 cut(s) 342, 409, 534, 608, 759, 828
BssMI GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 554
Bst2BI CACGAG 1 cut(s) 554
Bst2UI CCWGG 4 cut(s) 250, 263, 428, 485
Bst4CI ACNGT 3 cut(s) 191, 603, 675
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 697
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 2 cut(s) 120, 524
BstDSI CCRYGG 2 cut(s) 608, 828
BstFNI CGCG 5 cut(s) 365, 375, 668, 687, 810
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 3 cut(s) 341, 377, 806
BstKTI GATC 7 cut(s) 10, 402, 408, 468, 547, 597, 666
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
BstMWI GCNNNNNNNGC 5 cut(s) 239, 374, 630, 801, 839
BstNI CCWGG 4 cut(s) 250, 263, 428, 485
BstSCI CCNGG 7 cut(s) 74, 248, 261, 341, 426, 483, 704
BstSLI GKGCMC 2 cut(s) 523, 696
BstUI CGCG 5 cut(s) 365, 375, 668, 687, 810
BstV1I GCAGC 2 cut(s) 100, 477
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 5 cut(s) 242, 266, 521, 704, 758
BtgI CCRYGG 2 cut(s) 608, 828
BtgZI GCGATG 1 cut(s) 831
BtsIMutI CAGTG 1 cut(s) 671
Cac8I GCNNGC 1 cut(s) 808
CaiI CAGNNNCTG 1 cut(s) 490
CfoI GCGC 3 cut(s) 341, 377, 806
Cfr13I GGNCC 5 cut(s) 388, 424, 519, 520, 703
CseI GACGC 2 cut(s) 119, 239
CsiI ACCWGGT 1 cut(s) 483
Csp6I GTAC 2 cut(s) 187, 628
CspCI CAANNNNNGTGG 2 cut(s) 542, 577
CviAII CATG 2 cut(s) 728, 844
CviQI GTAC 2 cut(s) 187, 628
DdeI CTNAG 2 cut(s) 120, 524
DpnI GATC 7 cut(s) 9, 401, 407, 467, 546, 596, 665
DpnII GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
EaeI YGGCCR 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 523
Eco47I GGWCC 2 cut(s) 388, 424
Eco72I CACGTG 1 cut(s) 697
Eco88I CYCGRG 2 cut(s) 200, 533
EcoO109I RGGNCCY 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 785
EcoRII CCWGG 4 cut(s) 248, 261, 426, 483
EcoT38I GRGCYC 1 cut(s) 523
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 2 cut(s) 731, 847
FaiI YATR 4 cut(s) 575, 729, 771, 845
FatI CATG 2 cut(s) 727, 843
FauI CCCGC 4 cut(s) 223, 617, 648, 680
Fnu4HI GCNGC 6 cut(s) 114, 219, 366, 491, 634, 669
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 6 cut(s) 114, 219, 366, 491, 634, 669
FspBI CTAG 2 cut(s) 170, 336
FspI TGCGCA 1 cut(s) 805
GlaI GCGC 3 cut(s) 340, 376, 805
GluI GCNGC 6 cut(s) 114, 219, 366, 491, 634, 669
GsaI CCCAGC 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 5 cut(s) 242, 266, 521, 704, 758
HapII CCGG 3 cut(s) 76, 342, 706
HgaI GACGC 2 cut(s) 119, 239
HhaI GCGC 3 cut(s) 341, 377, 806
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 731, 847
Hin6I GCGC 3 cut(s) 339, 375, 804
HinP1I GCGC 3 cut(s) 339, 375, 804
HincII GTYRAC 2 cut(s) 448, 478
HindII GTYRAC 2 cut(s) 448, 478
HinfI GANTC 4 cut(s) 284, 458, 526, 680
HpaII CCGG 3 cut(s) 76, 342, 706
HphI GGTGA 3 cut(s) 64, 238, 596
Hpy166II GTNNAC 5 cut(s) 358, 388, 448, 478, 694
Hpy188I TCNGA 4 cut(s) 382, 410, 463, 599
Hpy188III TCNNGA 3 cut(s) 325, 403, 548
Hpy8I GTNNAC 5 cut(s) 358, 388, 448, 478, 694
Hpy99I CGWCG 5 cut(s) 113, 185, 230, 233, 281
HpyAV CCTTC 2 cut(s) 585, 652
HpyCH4III ACNGT 3 cut(s) 191, 603, 675
HpyCH4IV ACGT 1 cut(s) 696
HpyCH4V TGCA 1 cut(s) 694
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 374, 630, 801, 839
HpyF3I CTNAG 2 cut(s) 120, 524
HpySE526I ACGT 1 cut(s) 696
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 731, 847
HspAI GCGC 3 cut(s) 339, 375, 804
Kzo9I GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
LmnI GCTCC 2 cut(s) 84, 736
Lsp1109I GCAGC 2 cut(s) 100, 477
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 2 cut(s) 170, 336
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 2 cut(s) 548, 681
MalI GATC 7 cut(s) 9, 401, 407, 467, 546, 596, 665
MboI GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 3 cut(s) 207, 523, 696
MluCI AATT 2 cut(s) 86, 785
MlyI GAGTC 2 cut(s) 535, 689
MmeI TCCRAC 1 cut(s) 657
MseI TTAA 2 cut(s) 711, 780
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 3 cut(s) 76, 342, 706
MspR9I CCNGG 7 cut(s) 76, 250, 263, 343, 428, 485, 706
MvaI CCWGG 4 cut(s) 250, 263, 428, 485
MvnI CGCG 5 cut(s) 365, 375, 668, 687, 810
MwoI GCNNNNNNNGC 5 cut(s) 239, 374, 630, 801, 839
NciI CCSGG 3 cut(s) 76, 343, 706
NdeII GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
NlaIII CATG 2 cut(s) 731, 847
NlaIV GGNNCC 2 cut(s) 80, 521
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 2 cut(s) 548, 681
NsbI TGCGCA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 2 cut(s) 284, 458
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 6 cut(s) 115, 220, 367, 492, 635, 670
PleI GAGTC 2 cut(s) 534, 688
PmaCI CACGTG 1 cut(s) 697
PmlI CACGTG 1 cut(s) 697
PpsI GAGTC 2 cut(s) 534, 688
Ppu21I YACGTR 1 cut(s) 697
Psp6I CCWGG 4 cut(s) 248, 261, 426, 483
PspCI CACGTG 1 cut(s) 697
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 4 cut(s) 248, 261, 426, 483
PspN4I GGNNCC 2 cut(s) 80, 521
PspOMI GGGCCC 1 cut(s) 519
PspPI GGNCC 5 cut(s) 388, 424, 519, 520, 703
PstNI CAGNNNCTG 1 cut(s) 490
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 2 cut(s) 188, 629
RsaNI GTAC 2 cut(s) 187, 628
SaqAI TTAA 2 cut(s) 711, 780
SatI GCNGC 6 cut(s) 114, 219, 366, 491, 634, 669
Sau3AI GATC 7 cut(s) 7, 399, 405, 465, 544, 594, 663
Sau96I GGNCC 5 cut(s) 388, 424, 519, 520, 703
SchI GAGTC 2 cut(s) 535, 689
ScrFI CCNGG 7 cut(s) 76, 250, 263, 343, 428, 485, 706
SduI GDGCHC 3 cut(s) 207, 523, 696
SetI ASST 6 cut(s) 251, 393, 473, 489, 699, 741
SexAI ACCWGGT 1 cut(s) 483
SinI GGWCC 2 cut(s) 388, 424
Sse9I AATT 2 cut(s) 86, 785
SseBI AGGCCT 1 cut(s) 266
SspMI CTAG 2 cut(s) 170, 336
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 7 cut(s) 74, 248, 261, 341, 426, 483, 704
TaaI ACNGT 3 cut(s) 191, 603, 675
TaiI ACGT 1 cut(s) 699
TaqI TCGA 3 cut(s) 24, 183, 276
TasI AATT 2 cut(s) 86, 785
TauI GCSGC 4 cut(s) 221, 368, 636, 671
TfiI GAWTC 2 cut(s) 284, 458
Tru1I TTAA 2 cut(s) 711, 780
Tru9I TTAA 2 cut(s) 711, 780
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 2 cut(s) 548, 681
TseI GCWGC 2 cut(s) 113, 490
Tsp45I GTSAC 2 cut(s) 548, 681
TspDTI ATGAA 3 cut(s) 590, 716, 778
TspGWI ACGGA 5 cut(s) 169, 292, 294, 470, 558
TspRI CASTG 1 cut(s) 678
Van91I CCANNNNNTGG 1 cut(s) 249
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 2 cut(s) 388, 424
XapI RAATTY 1 cut(s) 785
XspI CTAG 2 cut(s) 170, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.