FvH4_7g12500

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005485
Physical Location & Seq
Forward (+)
11325724 .. 11327627
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12500.t1

Sequence Viewer

Length: 1011 bp
ATGGCAGATCAACCATCCTCACCCGACCCCTACAAGCTCCTCCACATCGTTCCCAACCCAGACGGCTCCCTCACCCGCCTCCCCACAACCGAGATAGTCCCCGCCGGCGCCTCCGATCCCAACTCACCCCAACTCGTCCTCTCCAAAGACATCCCTCTCGACCCTATACACCAAACCTATCTCCGCCTCTTCAAACCCCACAACCTCCCGCCAAACCCCAACCTCCCTCTCCTCATCTACTTCCACGGTGGCGGCTTCGTCCTCTCAAGCGCCGCCTCCGCGCCTTTCCACGACTGCTGCTCCCGCCTCGCCCTCTCCATCCCCGCCATAGTCATCTCCGTCGAGTACCGCCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGCCGTCTCCGTCATCTCCTGGGTCAGAAACCAAGCCTCCGCCGCCGACGGAGAACCGTGGCTGAAACACTACGTTGACTTCTCCAAGTGTTTCTTAATGGGATGTAGCGCGGGGGGTAATATCGTCTACCACGCGGGTCTACGCGCTTCCGATATGGACCTCTCCCCGGTCAAGATCCAAGGGCTGATATTGCACCAGCCGTATTTCGGTGGGGTCCGCAGGACGGAGTCGGAGACCAGGTTCGTCAACGACCGCGTGATACCGTTAGTTGCAAACGACTTGCTGTGGGAGCTGAGTCTGCCGGAGGGGGCTGACCGTGACCATGAGTATAGTAACCCGTTAATTAACGGCGGCGACGGGAGGATCAGACGGCTGCCGAGGTGTTTGGTGTGTGGGTACGGTGGGGATCCATTGGTGGATAGGCAGAAGGAGTTGGCGGGTCTGTTGGAATCACGCGGGGTGCACGTGACGGCTCAGTTCAAGGAGGGTGGTTTTCATGGTGTGGATGTATTTGACCCGATTAAGGCCGAGGCTTTATATGAGAGTGTTAAGGAATTTATCGGCGGCTCTTGTTGTGCACAAGATGGTGATGAGGCTGTTGTTGCAAAATCGAGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

337

Amino Acids

36.55

Weight (kDa)

5.51

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 73 - 181 2.6e-12 BD-FAE
Abhydrolase_3 PF07859 78 - 298 9.8e-58 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 2 cut(s) 516, 529
AccII CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
AclWI GGATC 5 cut(s) 110, 559, 761, 791, 804
AcsI RAATTY 1 cut(s) 944
AcvI CACGTG 1 cut(s) 856
AcyI GRCGYC 2 cut(s) 108, 390
AfaI GTAC 2 cut(s) 347, 788
AfiI CCNNNNNNNGG 4 cut(s) 556, 596, 613, 913
AgsI TTSAA 2 cut(s) 193, 871
AjnI CCWGG 2 cut(s) 407, 626
AloI GAACNNNNNNTCC 2 cut(s) 614, 646
AluBI AGCT 2 cut(s) 37, 682
AluI AGCT 2 cut(s) 37, 682
Alw21I GWGCWC 3 cut(s) 366, 855, 970
Alw26I GTCTC 2 cut(s) 400, 617
Alw44I GTGCAC 2 cut(s) 851, 966
AlwI GGATC 5 cut(s) 110, 559, 761, 791, 804
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 1 cut(s) 915
ApaLI GTGCAC 2 cut(s) 851, 966
ApeKI GCWGC 2 cut(s) 297, 763
ApoI RAATTY 1 cut(s) 944
AspLEI GCGC 5 cut(s) 110, 272, 283, 500, 536
AspS9I GGNCC 2 cut(s) 547, 604
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 4 cut(s) 12, 64, 117, 989
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 2 cut(s) 547, 604
BaeGI GKGCMC 2 cut(s) 855, 970
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 107
BbrPI CACGTG 1 cut(s) 856
Bbv12I GWGCWC 3 cut(s) 366, 855, 970
BbvI GCAGC 2 cut(s) 284, 750
BccI CCATC 3 cut(s) 22, 326, 968
BceAI ACGGC 6 cut(s) 79, 377, 574, 754, 776, 876
BciT130I CCWGG 2 cut(s) 409, 628
BcnI CCSGG 1 cut(s) 557
BcoDI GTCTC 2 cut(s) 400, 617
BfoI RGCGCY 2 cut(s) 111, 273
BisI GCNGC 8 cut(s) 253, 273, 298, 378, 432, 742, 764, 955
BlsI GCNGC 8 cut(s) 254, 274, 299, 379, 433, 743, 765, 956
Bme1390I CCNGG 3 cut(s) 409, 557, 628
Bme18I GGWCC 2 cut(s) 547, 604
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 2 cut(s) 547, 604
BmiI GGNNCC 4 cut(s) 67, 109, 605, 798
BmrFI CCNGG 3 cut(s) 409, 557, 628
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 557
BsaAI YACGTR 1 cut(s) 856
BsaHI GRCGYC 2 cut(s) 108, 390
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 7 cut(s) 244, 408, 446, 555, 568, 767, 918
BsaXI ACNNNNNCTCC 8 cut(s) 284, 314, 380, 410, 410, 440, 614, 644
Bsc4I CCNNNNNNNGG 4 cut(s) 556, 596, 613, 913
Bse118I RCCGGY 1 cut(s) 104
BseBI CCWGG 2 cut(s) 409, 628
BseDI CCNNGG 7 cut(s) 244, 408, 446, 555, 568, 767, 918
BseGI GGATG 5 cut(s) 14, 150, 318, 497, 901
BseLI CCNNNNNNNGG 4 cut(s) 556, 596, 613, 913
BseMII CTCAG 2 cut(s) 674, 878
BseRI GAGGAG 2 cut(s) 29, 221
BseSI GKGCMC 2 cut(s) 855, 970
BseXI GCAGC 2 cut(s) 284, 750
Bsh1236I CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 1 cut(s) 917
BshNI GGYRCC 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 3 cut(s) 366, 855, 970
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 3 cut(s) 105, 557, 692
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 4 cut(s) 556, 596, 613, 913
BsmAI GTCTC 2 cut(s) 400, 617
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 1 cut(s) 917
Bso31I GGTCTC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 366, 855, 970
Bsp143I GATC 5 cut(s) 7, 115, 564, 753, 796
BspANI GGCC 1 cut(s) 917
BspCNI CTCAG 2 cut(s) 675, 877
BspFNI CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
BspLI GGNNCC 4 cut(s) 67, 109, 605, 798
BspPI GGATC 5 cut(s) 110, 559, 761, 791, 804
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 7 cut(s) 244, 408, 446, 555, 568, 767, 918
BssMI GATC 5 cut(s) 7, 115, 564, 753, 796
BssNI GRCGYC 2 cut(s) 108, 390
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 409, 628
Bst4CI ACNGT 5 cut(s) 248, 447, 654, 707, 791
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 2 cut(s) 108, 390
BstBAI YACGTR 1 cut(s) 856
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 2 cut(s) 683, 864
BstDSI CCRYGG 2 cut(s) 244, 446
BstF5I GGATG 5 cut(s) 14, 150, 318, 497, 901
BstFNI CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
BstH2I RGCGCY 2 cut(s) 111, 273
BstHHI GCGC 5 cut(s) 110, 272, 283, 500, 536
BstKTI GATC 5 cut(s) 10, 118, 567, 756, 799
BstMAI GTCTC 2 cut(s) 400, 617
BstMBI GATC 5 cut(s) 7, 115, 564, 753, 796
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 7 cut(s) 278, 303, 431, 580, 679, 688, 992
BstNI CCWGG 2 cut(s) 409, 628
BstSCI CCNGG 3 cut(s) 407, 555, 626
BstSLI GKGCMC 2 cut(s) 855, 970
BstUI CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
BstV1I GCAGC 2 cut(s) 284, 750
BstX2I RGATCY 2 cut(s) 564, 796
BstYI RGATCY 2 cut(s) 564, 796
BsuRI GGCC 1 cut(s) 917
BtgI CCRYGG 2 cut(s) 244, 446
BtsCI GGATG 5 cut(s) 14, 150, 318, 497, 901
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 5 cut(s) 110, 272, 283, 500, 536
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 547, 604
CseI GACGC 1 cut(s) 398
CsiI ACCWGGT 1 cut(s) 626
Csp6I GTAC 2 cut(s) 346, 787
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 2 cut(s) 713, 887
CviQI GTAC 2 cut(s) 346, 787
DdeI CTNAG 2 cut(s) 683, 864
DinI GGCGCC 1 cut(s) 109
DpnI GATC 5 cut(s) 9, 117, 566, 755, 798
DpnII GATC 5 cut(s) 7, 115, 564, 753, 796
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 2 cut(s) 173, 418
Eco130I CCWWGG 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 617
Eco47I GGWCC 2 cut(s) 547, 604
Eco72I CACGTG 1 cut(s) 856
Eco88I CYCGRG 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 407, 626
EcoT14I CCWWGG 1 cut(s) 568
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 568
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 2 cut(s) 716, 890
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 83
FatI CATG 2 cut(s) 712, 886
FauNDI CATATG 1 cut(s) 1007
FblI GTMKAC 2 cut(s) 516, 529
Fnu4HI GCNGC 8 cut(s) 253, 273, 298, 378, 432, 742, 764, 955
FokI GGATG 4 cut(s) 137, 305, 504, 908
Fsp4HI GCNGC 8 cut(s) 253, 273, 298, 378, 432, 742, 764, 955
GlaI GCGC 5 cut(s) 109, 271, 282, 499, 535
GluI GCNGC 8 cut(s) 253, 273, 298, 378, 432, 742, 764, 955
HaeII RGCGCY 2 cut(s) 111, 273
HaeIII GGCC 1 cut(s) 917
HapII CCGG 3 cut(s) 105, 557, 692
HgaI GACGC 1 cut(s) 398
HhaI GCGC 5 cut(s) 110, 272, 283, 500, 536
Hin1I GRCGYC 2 cut(s) 108, 390
Hin1II CATG 2 cut(s) 716, 890
Hin6I GCGC 5 cut(s) 108, 270, 281, 498, 534
HinP1I GCGC 5 cut(s) 108, 270, 281, 498, 534
HincII GTYRAC 2 cut(s) 466, 637
HindII GTYRAC 2 cut(s) 466, 637
HinfI GANTC 3 cut(s) 617, 685, 839
HpaII CCGG 3 cut(s) 105, 557, 692
HphI GGTGA 4 cut(s) 12, 64, 117, 989
Hpy166II GTNNAC 6 cut(s) 466, 517, 530, 637, 853, 968
Hpy188I TCNGA 5 cut(s) 115, 416, 541, 622, 758
Hpy188III TCNNGA 2 cut(s) 158, 562
Hpy8I GTNNAC 6 cut(s) 466, 517, 530, 637, 853, 968
Hpy99I CGWCG 5 cut(s) 344, 389, 392, 440, 749
HpyAV CCTTC 1 cut(s) 811
HpyCH4III ACNGT 5 cut(s) 248, 447, 654, 707, 791
HpyCH4IV ACGT 2 cut(s) 462, 855
HpyCH4V TGCA 5 cut(s) 583, 662, 853, 968, 995
HpyF10VI GCNNNNNNNGC 7 cut(s) 278, 303, 431, 580, 679, 688, 992
HpyF3I CTNAG 2 cut(s) 683, 864
HpySE526I ACGT 2 cut(s) 462, 855
Hsp92I GRCGYC 2 cut(s) 108, 390
Hsp92II CATG 2 cut(s) 716, 890
HspAI GCGC 5 cut(s) 108, 270, 281, 498, 534
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 5 cut(s) 7, 115, 564, 753, 796
LmnI GCTCC 4 cut(s) 42, 71, 305, 679
Lsp1109I GCAGC 2 cut(s) 284, 750
MabI ACCWGGT 1 cut(s) 626
MaeII ACGT 2 cut(s) 462, 855
MaeIII GTNAC 3 cut(s) 707, 722, 856
MalI GATC 5 cut(s) 9, 117, 566, 755, 798
MboI GATC 5 cut(s) 7, 115, 564, 753, 796
MboII GAAGA 1 cut(s) 181
MflI RGATCY 2 cut(s) 564, 796
MhlI GDGCHC 3 cut(s) 366, 855, 970
MluCI AATT 2 cut(s) 732, 944
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 2 cut(s) 626, 694
MmeI TCCRAC 2 cut(s) 600, 816
MreI CGCCGGCG 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 5 cut(s) 485, 731, 735, 912, 939
MspI CCGG 3 cut(s) 105, 557, 692
MspR9I CCNGG 3 cut(s) 409, 557, 628
MvaI CCWGG 2 cut(s) 409, 628
MvnI CGCG 6 cut(s) 281, 500, 524, 534, 645, 846
MwoI GCNNNNNNNGC 7 cut(s) 278, 303, 431, 580, 679, 688, 992
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 1 cut(s) 557
NdeI CATATG 1 cut(s) 1007
NdeII GATC 5 cut(s) 7, 115, 564, 753, 796
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 2 cut(s) 716, 890
NlaIV GGNNCC 4 cut(s) 67, 109, 605, 798
NmeAIII GCCGAG 2 cut(s) 792, 943
NmuCI GTSAC 2 cut(s) 707, 856
PacI TTAATTAA 1 cut(s) 735
PcsI WCGNNNNNNNCGW 2 cut(s) 390, 396
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 839
PflFI GACNNNGTC 2 cut(s) 392, 616
PkrI GCNGC 8 cut(s) 254, 274, 299, 379, 433, 743, 765, 956
PleI GAGTC 2 cut(s) 625, 693
PluTI GGCGCC 1 cut(s) 111
PmaCI CACGTG 1 cut(s) 856
PmlI CACGTG 1 cut(s) 856
PpsI GAGTC 2 cut(s) 625, 693
Ppu21I YACGTR 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 407, 626
PspCI CACGTG 1 cut(s) 856
PspGI CCWGG 2 cut(s) 407, 626
PspN4I GGNNCC 4 cut(s) 67, 109, 605, 798
PspPI GGNCC 2 cut(s) 547, 604
PsuI RGATCY 2 cut(s) 564, 796
PsyI GACNNNGTC 2 cut(s) 392, 616
RsaI GTAC 2 cut(s) 347, 788
RsaNI GTAC 2 cut(s) 346, 787
SaqAI TTAA 5 cut(s) 485, 731, 735, 912, 939
SatI GCNGC 8 cut(s) 253, 273, 298, 378, 432, 742, 764, 955
Sau3AI GATC 5 cut(s) 7, 115, 564, 753, 796
Sau96I GGNCC 2 cut(s) 547, 604
SchI GAGTC 2 cut(s) 626, 694
ScrFI CCNGG 3 cut(s) 409, 557, 628
SduI GDGCHC 3 cut(s) 366, 855, 970
SexAI ACCWGGT 1 cut(s) 626
SfoI GGCGCC 1 cut(s) 109
SgrAI CRCCGGYG 1 cut(s) 104
SinI GGWCC 2 cut(s) 547, 604
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 2 cut(s) 732, 944
SspDI GGCGCC 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 407, 555, 626
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 5 cut(s) 248, 447, 654, 707, 791
TaiI ACGT 2 cut(s) 465, 858
TaqI TCGA 3 cut(s) 159, 342, 1001
TasI AATT 2 cut(s) 732, 944
TauI GCSGC 6 cut(s) 255, 275, 380, 434, 744, 957
TfiI GAWTC 1 cut(s) 839
Tru1I TTAA 5 cut(s) 485, 731, 735, 912, 939
Tru9I TTAA 5 cut(s) 485, 731, 735, 912, 939
TseFI GTSAC 2 cut(s) 707, 856
TseI GCWGC 2 cut(s) 297, 763
Tsp45I GTSAC 2 cut(s) 707, 856
TspDTI ATGAA 1 cut(s) 875
TspGWI ACGGA 4 cut(s) 328, 388, 453, 629
Tth111I GACNNNGTC 2 cut(s) 392, 616
VneI GTGCAC 2 cut(s) 851, 966
VpaK11BI GGWCC 2 cut(s) 547, 604
XapI RAATTY 1 cut(s) 944
XmiI GTMKAC 2 cut(s) 516, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.