pycom02g16210

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005485
Physical Location & Seq
Reverse (-)
13367543 .. 13368601
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16210.1

Sequence Viewer

Length: 1059 bp
ATGGCGGATCAACCTTCTTCATCCCCAACCTCCATTGACCCCTACAAACTCCTCAAAATCCTCCCCAACCCTGATGGCTCCCTCACCCGCTTGACCACTTTCCCCACCGTCTCCCCAACTCCCACCCCCACCGAGTCCATTTCCGCCACGGCCGCCGAGTCAGACTCCAACTCTCCCCAACTCGTCCTCTCCAAGGACATTCCCTTAAACCCAGAAACAAAAACCTTCTTGAGGGTCTTCAAACCCCACCCTCTCCCCCCAAACCCCCGCCTCCCTCTCATCATCTACTTCCACGGAGGCGGCTTCGTCCTCTTCTCCGCCGCCTCCAAACCATTCCACGACTCCTGCTCTAGCATGGCCCTCGCTCTCCAAGCCCTAATTATCTCCGTCGAGTACCGCTTGGCTCCCGAACACCCTCTTCCTTCAGCCTACGATGACGCCGTCGACACCATCGCCTGGGTCCAGCGCCAGGCCTCCGACGTCGACGGCTGCGATCCCTGGTTGAAAGACGCCGTTGACTTCTCCAGGTGCTTCCTAATGGGCAGCAGCGCCGGAGCTAACATCAGCTACCACGTGGGTTTACGCCTCTCGGGTGATGATCTCTTGCCGATGAAGATCCGAGGGCTGATACTAAACCAGCCTTATTTCGGCGGGGTCCATAGGACTGAATCCGAGACGAGGTTGATCAACGACCGGATATTGCCGCTGGTCGCCACCGACTTGCTGTGGGCAATGGCCTTGCCAAAGGGCGTTGATCGGGATCATGAGTATTGTAATCCGACGGCGGGAGGTGGGGATGGGAGGATCGAACGGTTGCCGAGGTGTTTGGTTAGAGGGTACGGTGGGGACCCGCTGATGGATAGGCAGATGGAGTTTGCGGCGTTACTTGAGTCACGTGGGGCGCACGTGGTGGCCAAGTTTGACGAGGGGGGTTATCATAGCGTGGAGCTCTTCGACCCTAGCATGGCCCAAGCTCTGTACGACATTATTAGGGATTTTATCGAGTCGTGCGATGCAGACGACGGTGATAATATTGTCGTTTCAAAATCGGCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

353

Amino Acids

38.38

Weight (kDa)

4.96

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 90 - 195 3.6e-10 BD-FAE
Abhydrolase_3 PF07859 94 - 315 1e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 483
AccB7I CCANNNNNTGG 1 cut(s) 456
AccI GTMKAC 2 cut(s) 444, 483
AclWI GGATC 5 cut(s) 15, 488, 610, 768, 812
AcoI YGGCCR 2 cut(s) 150, 912
AcuI CTGAAG 1 cut(s) 408
AcvI CACGTG 3 cut(s) 574, 896, 907
AcyI GRCGYC 3 cut(s) 438, 480, 510
AdeI CACNNNGTG 1 cut(s) 910
AfaI GTAC 3 cut(s) 395, 839, 980
AfiI CCNNNNNNNGG 9 cut(s) 193, 231, 456, 469, 647, 678, 785, 856, 964
AgsI TTSAA 3 cut(s) 241, 505, 1044
AjnI CCWGG 4 cut(s) 455, 468, 497, 524
AluBI AGCT 4 cut(s) 557, 567, 949, 974
AluI AGCT 4 cut(s) 557, 567, 949, 974
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 115, 668
AlwI GGATC 5 cut(s) 15, 488, 610, 768, 812
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 6 cut(s) 150, 357, 471, 735, 912, 966
ApeKI GCWGC 3 cut(s) 489, 543, 546
AspLEI GCGC 3 cut(s) 468, 551, 904
AspS9I GGNCC 5 cut(s) 358, 460, 655, 847, 967
AsuHPI GGTGA 3 cut(s) 76, 605, 1037
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 3 cut(s) 460, 655, 847
BalI TGGCCA 1 cut(s) 914
BanII GRGCYC 1 cut(s) 951
BbrPI CACGTG 3 cut(s) 574, 896, 907
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 3 cut(s) 476, 555, 558
BccI CCATC 5 cut(s) 68, 458, 791, 850, 862
BceAI ACGGC 5 cut(s) 165, 425, 497, 502, 798
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 4 cut(s) 457, 470, 499, 526
BclI TGATCA 1 cut(s) 684
BcoDI GTCTC 2 cut(s) 115, 668
BfaI CTAG 2 cut(s) 351, 960
BfoI RGCGCY 2 cut(s) 469, 552
BisI GCNGC 8 cut(s) 153, 301, 321, 490, 544, 547, 704, 879
BlsI GCNGC 8 cut(s) 154, 302, 322, 491, 545, 548, 705, 880
Bme1390I CCNGG 4 cut(s) 457, 470, 499, 526
Bme18I GGWCC 3 cut(s) 460, 655, 847
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 5 cut(s) 358, 460, 655, 847, 967
BmiI GGNNCC 6 cut(s) 79, 405, 461, 656, 848, 849
BmrFI CCNGG 4 cut(s) 457, 470, 499, 526
BmsI GCATC 1 cut(s) 1003
BpiI GAAGAC 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 508
BpuEI CTTGAG 2 cut(s) 250, 908
BsaAI YACGTR 3 cut(s) 574, 896, 907
BsaBI GATNNNNATC 2 cut(s) 614, 759
BsaHI GRCGYC 3 cut(s) 438, 480, 510
BsaJI CCNNGG 7 cut(s) 147, 192, 292, 456, 497, 619, 818
BsaWI WCCGGW 1 cut(s) 693
Bsc4I CCNNNNNNNGG 9 cut(s) 193, 231, 456, 469, 647, 678, 785, 856, 964
Bse3DI GCAATG 1 cut(s) 738
Bse8I GATNNNNATC 2 cut(s) 614, 759
BseBI CCWGG 4 cut(s) 457, 470, 499, 526
BseDI CCNNGG 7 cut(s) 147, 192, 292, 456, 497, 619, 818
BseGI GGATG 2 cut(s) 20, 802
BseJI GATNNNNATC 2 cut(s) 614, 759
BseLI CCNNNNNNNGG 9 cut(s) 193, 231, 456, 469, 647, 678, 785, 856, 964
BseMI GCAATG 1 cut(s) 738
BseRI GAGGAG 1 cut(s) 41
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 3 cut(s) 476, 555, 558
Bsh1285I CGRYCG 2 cut(s) 153, 694
BshFI GGCC 6 cut(s) 152, 359, 473, 737, 914, 968
BsiEI CGRYCG 2 cut(s) 153, 694
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 2 cut(s) 552, 694
BslFI GGGAC 1 cut(s) 860
BslI CCNNNNNNNGG 9 cut(s) 193, 231, 456, 469, 647, 678, 785, 856, 964
BsmAI GTCTC 2 cut(s) 115, 668
BsmBI CGTCTC 2 cut(s) 115, 668
BsmFI GGGAC 1 cut(s) 860
BsnI GGCC 6 cut(s) 152, 359, 473, 737, 914, 968
BsoBI CYCGRG 1 cut(s) 589
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
BspANI GGCC 6 cut(s) 152, 359, 473, 737, 914, 968
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 6 cut(s) 79, 405, 461, 656, 848, 849
BspPI GGATC 5 cut(s) 15, 488, 610, 768, 812
BspQI GCTCTTC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 738
BssECI CCNNGG 7 cut(s) 147, 192, 292, 456, 497, 619, 818
BssMI GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
BssNI GRCGYC 3 cut(s) 438, 480, 510
BssT1I CCWWGG 1 cut(s) 192
Bst2UI CCWGG 4 cut(s) 457, 470, 499, 526
Bst4CI ACNGT 4 cut(s) 109, 813, 842, 1025
Bst6I CTCTTC 3 cut(s) 317, 423, 956
BstACI GRCGYC 3 cut(s) 438, 480, 510
BstBAI YACGTR 3 cut(s) 574, 896, 907
BstDSI CCRYGG 2 cut(s) 147, 292
BstENI CCTNNNNNAGG 2 cut(s) 191, 229
BstF5I GGATG 2 cut(s) 20, 802
BstH2I RGCGCY 2 cut(s) 469, 552
BstHHI GCGC 3 cut(s) 468, 551, 904
BstKTI GATC 8 cut(s) 10, 496, 601, 618, 687, 757, 763, 807
BstMAI GTCTC 2 cut(s) 115, 668
BstMBI GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
BstMCI CGRYCG 2 cut(s) 153, 694
BstMWI GCNNNNNNNGC 2 cut(s) 152, 371
BstNI CCWGG 4 cut(s) 457, 470, 499, 526
BstSCI CCNGG 4 cut(s) 455, 468, 497, 524
BstV1I GCAGC 3 cut(s) 476, 555, 558
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 615
BstYI RGATCY 1 cut(s) 615
BstZI CGGCCG 1 cut(s) 150
BsuRI GGCC 6 cut(s) 152, 359, 473, 737, 914, 968
BtgI CCRYGG 2 cut(s) 147, 292
BtgZI GCGATG 2 cut(s) 436, 1026
BtsCI GGATG 2 cut(s) 20, 802
CciI TCATGA 1 cut(s) 763
CfoI GCGC 3 cut(s) 468, 551, 904
Cfr13I GGNCC 5 cut(s) 358, 460, 655, 847, 967
CseI GACGC 2 cut(s) 446, 518
Csp6I GTAC 3 cut(s) 394, 838, 979
CviAII CATG 3 cut(s) 355, 764, 964
CviQI GTAC 3 cut(s) 394, 838, 979
DpnI GATC 8 cut(s) 9, 495, 600, 617, 686, 756, 762, 806
DpnII GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
DraIII CACNNNGTG 1 cut(s) 910
EaeI YGGCCR 2 cut(s) 150, 912
EagI CGGCCG 1 cut(s) 150
Eam1104I CTCTTC 3 cut(s) 317, 423, 956
EarI CTCTTC 3 cut(s) 317, 423, 956
EciI GGCGGA 3 cut(s) 20, 133, 307
Ecl136II GAGCTC 1 cut(s) 949
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 192
Eco147I AGGCCT 1 cut(s) 473
Eco24I GRGCYC 1 cut(s) 951
Eco47I GGWCC 3 cut(s) 460, 655, 847
Eco52I CGGCCG 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 1 cut(s) 408
Eco72I CACGTG 3 cut(s) 574, 896, 907
Eco88I CYCGRG 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 949
EcoNI CCTNNNNNAGG 2 cut(s) 191, 229
EcoO109I RGGNCCY 1 cut(s) 847
EcoRII CCWGG 4 cut(s) 455, 468, 497, 524
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 2 cut(s) 115, 668
FaeI CATG 3 cut(s) 358, 767, 967
FaiI YATR 5 cut(s) 356, 660, 765, 939, 965
FaqI GGGAC 1 cut(s) 860
FatI CATG 3 cut(s) 354, 763, 963
FauI CCCGC 5 cut(s) 95, 275, 644, 778, 858
FbaI TGATCA 1 cut(s) 684
FblI GTMKAC 2 cut(s) 444, 483
Fnu4HI GCNGC 8 cut(s) 153, 301, 321, 490, 544, 547, 704, 879
FokI GGATG 2 cut(s) 7, 809
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 8 cut(s) 153, 301, 321, 490, 544, 547, 704, 879
FspBI CTAG 2 cut(s) 351, 960
GlaI GCGC 3 cut(s) 467, 550, 903
GluI GCNGC 8 cut(s) 153, 301, 321, 490, 544, 547, 704, 879
GsuI CTGGAG 1 cut(s) 508
HaeII RGCGCY 2 cut(s) 469, 552
HaeIII GGCC 6 cut(s) 152, 359, 473, 737, 914, 968
HapII CCGG 2 cut(s) 552, 694
HgaI GACGC 2 cut(s) 446, 518
HhaI GCGC 3 cut(s) 468, 551, 904
Hin1I GRCGYC 3 cut(s) 438, 480, 510
Hin1II CATG 3 cut(s) 358, 767, 967
Hin6I GCGC 3 cut(s) 466, 549, 902
HinP1I GCGC 3 cut(s) 466, 549, 902
HincII GTYRAC 3 cut(s) 445, 484, 517
HindII GTYRAC 3 cut(s) 445, 484, 517
HinfI GANTC 7 cut(s) 134, 158, 164, 341, 668, 890, 1004
HpaII CCGG 2 cut(s) 552, 694
HphI GGTGA 3 cut(s) 76, 605, 1037
Hpy166II GTNNAC 4 cut(s) 445, 484, 517, 581
Hpy188I TCNGA 5 cut(s) 163, 478, 620, 673, 780
Hpy188III TCNNGA 4 cut(s) 229, 407, 758, 764
Hpy8I GTNNAC 4 cut(s) 445, 484, 517, 581
Hpy99I CGWCG 7 cut(s) 392, 446, 482, 485, 488, 784, 1025
HpyAV CCTTC 3 cut(s) 24, 235, 432
HpyCH4III ACNGT 4 cut(s) 109, 813, 842, 1025
HpyCH4IV ACGT 4 cut(s) 480, 573, 895, 906
HpyCH4V TGCA 1 cut(s) 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 371
HpySE526I ACGT 4 cut(s) 480, 573, 895, 906
Hsp92I GRCGYC 3 cut(s) 438, 480, 510
Hsp92II CATG 3 cut(s) 358, 767, 967
HspAI GCGC 3 cut(s) 466, 549, 902
KflI GGGWCCC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
LguI GCTCTTC 1 cut(s) 956
LmnI GCTCC 4 cut(s) 83, 409, 554, 946
Lsp1109I GCAGC 3 cut(s) 476, 555, 558
LweI GCATC 1 cut(s) 1003
MaeI CTAG 2 cut(s) 351, 960
MaeII ACGT 4 cut(s) 480, 573, 895, 906
MaeIII GTNAC 2 cut(s) 882, 891
MalI GATC 8 cut(s) 9, 495, 600, 617, 686, 756, 762, 806
MboI GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
MboII GAAGA 6 cut(s) 9, 229, 304, 410, 625, 943
MflI RGATCY 1 cut(s) 615
MhlI GDGCHC 1 cut(s) 951
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 6 cut(s) 143, 158, 167, 335, 899, 1013
MmeI TCCRAC 3 cut(s) 192, 501, 803
Mox20I TGGCCA 1 cut(s) 914
MscI TGGCCA 1 cut(s) 914
MseI TTAA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 914
MspA1I CMGCKG 2 cut(s) 706, 853
MspI CCGG 2 cut(s) 552, 694
MspR9I CCNGG 4 cut(s) 457, 470, 499, 526
MvaI CCWGG 4 cut(s) 457, 470, 499, 526
MwoI GCNNNNNNNGC 2 cut(s) 152, 371
NdeII GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
NlaIII CATG 3 cut(s) 358, 767, 967
NlaIV GGNNCC 6 cut(s) 79, 405, 461, 656, 848, 849
NmeAIII GCCGAG 2 cut(s) 181, 843
NmuCI GTSAC 1 cut(s) 891
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 956
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 489, 1008
PfeI GAWTC 1 cut(s) 668
PflFI GACNNNGTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 8 cut(s) 154, 302, 322, 491, 545, 548, 705, 880
PleI GAGTC 6 cut(s) 142, 158, 166, 335, 898, 1012
PmaCI CACGTG 3 cut(s) 574, 896, 907
PmlI CACGTG 3 cut(s) 574, 896, 907
PpsI GAGTC 6 cut(s) 142, 158, 166, 335, 898, 1012
Ppu21I YACGTR 3 cut(s) 574, 896, 907
PpuMI RGGWCCY 1 cut(s) 847
Psp124BI GAGCTC 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 4 cut(s) 455, 468, 497, 524
PspCI CACGTG 3 cut(s) 574, 896, 907
PspGI CCWGG 4 cut(s) 455, 468, 497, 524
PspN4I GGNNCC 6 cut(s) 79, 405, 461, 656, 848, 849
PspPI GGNCC 5 cut(s) 358, 460, 655, 847, 967
PspPPI RGGWCCY 1 cut(s) 847
PsuI RGATCY 1 cut(s) 615
PsyI GACNNNGTC 1 cut(s) 440
RsaI GTAC 3 cut(s) 395, 839, 980
RsaNI GTAC 3 cut(s) 394, 838, 979
SacI GAGCTC 1 cut(s) 951
SalI GTCGAC 2 cut(s) 443, 482
SapI GCTCTTC 1 cut(s) 956
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 8 cut(s) 153, 301, 321, 490, 544, 547, 704, 879
Sau3AI GATC 8 cut(s) 7, 493, 598, 615, 684, 754, 760, 804
Sau96I GGNCC 5 cut(s) 358, 460, 655, 847, 967
SchI GAGTC 6 cut(s) 143, 158, 167, 335, 899, 1013
ScrFI CCNGG 4 cut(s) 457, 470, 499, 526
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 1 cut(s) 1003
SgrDI CGTCGACG 1 cut(s) 482
SinI GGWCC 3 cut(s) 460, 655, 847
SmlI CTYRAG 2 cut(s) 229, 887
SmoI CTYRAG 2 cut(s) 229, 887
Sse9I AATT 1 cut(s) 378
SseBI AGGCCT 1 cut(s) 473
SspI AATATT 1 cut(s) 1033
SspMI CTAG 2 cut(s) 351, 960
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 4 cut(s) 455, 468, 497, 524
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 4 cut(s) 109, 813, 842, 1025
TaiI ACGT 4 cut(s) 483, 576, 898, 909
TaqI TCGA 6 cut(s) 390, 444, 483, 807, 954, 1002
TasI AATT 1 cut(s) 378
TauI GCSGC 5 cut(s) 155, 303, 323, 706, 881
TfiI GAWTC 1 cut(s) 668
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TseFI GTSAC 1 cut(s) 891
TseI GCWGC 3 cut(s) 489, 543, 546
Tsp45I GTSAC 1 cut(s) 891
TspDTI ATGAA 2 cut(s) 9, 626
TspGWI ACGGA 2 cut(s) 309, 376
Tth111I GACNNNGTC 1 cut(s) 440
Van91I CCANNNNNTGG 1 cut(s) 456
VpaK11BI GGWCC 3 cut(s) 460, 655, 847
XagI CCTNNNNNAGG 2 cut(s) 191, 229
XmiI GTMKAC 2 cut(s) 444, 483
XspI CTAG 2 cut(s) 351, 960
ZraI GACGTC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.